4.5 Article

Design, simulation and characterisation of integrated optics for a microfabricated flow cytometer

期刊

OPTICS COMMUNICATIONS
卷 283, 期 9, 页码 1987-1992

出版社

ELSEVIER SCIENCE BV
DOI: 10.1016/j.optcom.2009.12.046

关键词

Integrated optics; Flow cytometry; Optical losses; Collimation of light

类别

资金

  1. RMST [EP/E016774/1]
  2. EPSRC [EP/E016774/1] Funding Source: UKRI
  3. NERC [noc010003, noc010013] Funding Source: UKRI
  4. Engineering and Physical Sciences Research Council [EP/E016774/1] Funding Source: researchfish
  5. Natural Environment Research Council [noc010013, noc010003] Funding Source: researchfish

向作者/读者索取更多资源

Flow cytometry is widely used for analyzing micro-particles such as cells and bacteria. Microfabricated flow cytometers promise reduced instrument size and cost with increased robustness and have application in medicine, life sciences and environmental metrology. Further miniaturisation and robustness can be achieved if integrated optics are used instead of traditional free space optics. We present designs simulation and experimental characterisation of integrated optics for a microfabricated cytometer made from SU-8 resin on a glass substrate. The optics constructed from combinations of optical fibres (positioned with microgrooves), waveguides, and microlenses enable analysis of scattered light and fluorescence from particles positioned near the centre of a microchannel using one dimensional sheath flow. Four different methods for directing the incident light onto the particles are examined and the optimum design discussed. (C) 2009 Elsevier B.V. All rights reserved.

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