Article
Cell Biology
Bin Yu, Qiaoyu Lin, Chao Huang, Boyan Zhang, Ying Wang, Qing Jiang, Chuanmao Zhang, Jing Yi
Summary: Research has shown that conjugation of SUMO2 with AurA at K258 in early mitosis promotes the kinase activity of AurA, while inhibition of deSUMOylation leads to increased kinase activity and abnormalities in spindle assembly and chromosome segregation.
JOURNAL OF CELL SCIENCE
(2021)
Article
Cell Biology
Eric M. C. Britigan, Jun Wan, Daniel K. Sam, Sarah E. Copeland, Amber L. Lasek, Laura C. F. Hrycyniak, Lei Wang, Anjon Audhya, Mark E. Burkard, Avtar Roopra, Beth A. Weaver
Summary: The increased expression of Aurora B protein reduces its kinase activity and causes defects in mitosis. The complexes of Aurora B and its binding partner INCENP achieve Aurora B activation through autophosphorylation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Aamir Ali, Chithran Vineethakumari, Cristina Lacasa, Jens Luders
Summary: In human cells, the microtubule polymerase ch-TOG promotes nucleation of microtubules at the interphase centrosome and the Golgi by transiently interacting with the microtubule nucleator gamma TuRC. This mechanism allows for the regulation of microtubule arrays organization.
NATURE COMMUNICATIONS
(2023)
Article
Oncology
Ginia Ghosh, Sandip Misra, Rachayeeta Ray, Sougata Ghosh Chowdhury, Parimal Karmakar
Summary: The phosphorylation-dependent interaction of PTEN with PLK1 and Aurora Kinase A is crucial for preventing aberrant chromosome segregation in metaphase. Wild-type PTEN significantly reduces cytological damage and maintains chromosome numbers, while phospho-mutant and phosphatase-dead PTEN show increased cytological damage markers and aberrant gamma-tubulin pole formation.
Article
Cell Biology
Kenta Tsuchiya, Gohta Goshima
Summary: In human colon cancer cells, four microtubule-associated proteins have been identified that are involved in gamma-tubulin-independent microtubule generation. These proteins can promote microtubule nucleation in the absence of gamma-tubulin, forming noncentriolar microtubule organizing centers. Depletion of certain proteins delays the formation of these centers, indicating their role in microtubule nucleation without gamma-tubulin.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Nelly Gareil, Alison Gervais, Nicolas Macaisne, Guillaume Chevreux, Julie C. Canman, Jessica Andreani, Julien Dumont
Summary: Cytoplasmic linker-associated proteins (CLASPs) maintain microtubule growth by promoting rescue and suppressing catastrophe. They play critical roles in cell migration, Golgi organization, kinetochores, and cell division. The conserved C-terminal domain (CTD) of CLASPs serves as a structural motif and facilitates protein interactions.
Article
Cell Biology
Sadia Sarwar, Viacheslav M. Morozov, Hamsa Purayil, Yehia Daaka, Alexander M. Ishov
Summary: Androgen ablation therapy is the standard treatment for newly diagnosed prostate cancer patients. However, the relapse of castration-resistant prostate cancer (CRPC) often leads to metastasis and disease lethality. Current therapies for metastatic CRPC are limited due to resistance to Taxanes. In this study, we found that inhibition of the mitotic checkpoint kinase Mps1 enhances the efficacy of Taxanes treatment, providing a potential new therapeutic target for managing therapy-resistant metastatic CRPC.
CELL DEATH & DISEASE
(2022)
Article
Cell Biology
Wonkyung Oh, Ting Ting Wu, Seo-Yeon Jeong, Ho Jin You, Jung-Hee Lee
Summary: CtIP plays a critical role in regulating mitosis by interacting with TPX2 to control spindle dynamics and regulate the concentration of Aurora A and microtubule intensity at the spindle poles. Depletion of CtIP may lead to improper execution of mitosis and result in chromosomal instability.
Article
Biochemistry & Molecular Biology
Agata Gluszek-Kustusz, Benjamin Craske, Thibault Legal, Toni McHugh, Julie P. Welburn
Summary: During mitosis, the microtubule crosslinker protein PRC1 recruits motor proteins CENP-E and Kif4A to the central spindle to coordinate chromosome segregation and cytokinesis. This recruitment is mediated by a conserved hydrophobic motif and is spatially and temporally regulated by CENP-E. The interaction between PRC1 and microtubule motor proteins is essential for controlling chromosome partitioning, maintaining central spindle integrity, and ensuring cytokinesis during anaphase.
Article
Biochemistry & Molecular Biology
Ivor Joseph, Juan Flores, Victoria Farrell, Justin Davis, Jared Bianchi-Smak, Qiang Feng, Sayantani Goswami, Xiang Lin, Zhi Wei, Kevin Tong, Zhaohui Feng, Michael P. Verzi, Edward M. Bonder, James R. Goldenring, Nan Gao
Summary: RAB11 small GTPases and associated recycling endosome are involved in regulating mitosis. However, the physiological significance of this regulation in mammalian tissues has not been observed. Using mouse models, researchers found that the absence of RAB11 family members (Rab11a and Rab11b) in the intestinal epithelium leads to defective cell cycle entry, mitotic arrest, and apoptosis, resulting in lethality within 3 days. Further experiments showed that Rab11 depletion affects mitotic spindle formation and cell death. Proteomic analysis revealed that Rab11a and Rab11b interact with mitotic spindle microtubule regulators. Disrupting Rab11 impairs bipolar spindle formation and cell division by affecting the function of the kinesin motor KIF11. These findings suggest that RAB11A and RAB11B redundantly control mitotic spindle function and intestinal progenitor cell division, which may be important for the homeostasis and renewal of other mammalian tissues.
Article
Biochemistry & Molecular Biology
Federica Polverino, Francesco D. Naso, Italia A. Asteriti, Valentina Palmerini, Divya Singh, Davide Valente, Alexander W. Bird, Alessandro Rosa, Marina Mapelli, Giulia Guarguaglini
Summary: Mitotic spindle orientation is a crucial process in cell division, with Aurora-A and TPX2 playing key roles in regulating NuMA localization and spindle alignment. The interaction between TPX2 and Aurora-A is necessary for proper spindle orientation, and both Aurora-A and TPX2 levels can impact the stability of microtubules and spindle orientation.
Article
Biology
Alex F. Thompson, Patrick R. Blackburn, Noah S. Arons, Sarah N. Stevens, Dusica Babovic-Vuksanovic, Jane B. Lian, Eric W. Klee, Jason Stumpff
Summary: This study reveals that mutations in KIF22 disrupt chromosome segregation in anaphase, leading to reduced proliferation and abnormal cell morphology. It demonstrates the significance of regulating KIF22 activity and maintaining force balance in anaphase.
Review
Cell Biology
Aamir Ali, P. Todd Stukenberg
Summary: Cell division events are regulated to occur in a distinct order. The traditional view is that events are ordered based on changes in Cyclin Dependent Kinase (CDK) activities. However, recent studies suggest that events are actually ordered based on the position of chromatids during anaphase. This positioning is regulated by a gradient of Aurora B kinase activity and Aurora A kinase activity.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Vilte Stonyte, Maria Mastrangelopoulou, Romy Timmer, Lilian Lindbergsengen, Marina Vietri, Coen Campsteijn, Beata Grallert
Summary: GCN2/eIF2 alpha K4 serves as an eIF2 alpha kinase in stress response and unexpectedly acts as a regulator of mitosis in unstressed cells. Its function in mitosis is not related to translation reprogramming, but through the regulation of PP1 alpha and gamma, which were previously unidentified substrates. Inhibition of GCN2 or Aurora A leads to mitotic errors and cell death, suggesting the potential therapeutic utility of GCN2 inhibitors. These findings provide insights into the timely regulation of phosphorylation events during early mitosis.
Article
Cell Biology
Inmaculada Ayala, Antonino Colanzi
Summary: The Golgi complex plays a central role in secretory traffic and can form a structure called the Golgi ribbon. The balanced formation and cleavage of membrane tubules connecting the stacks are crucial for cell division and spindle formation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Annalisa Verrico, Paola Rovella, Laura Di Francesco, Michela Damizia, David Sasah Staid, Loredana Le Pera, M. Eugenia Schinina, Patrizia Lavia
Article
Neurosciences
Alfonso Grimaldi, Natalia Pediconi, Francesca Oieni, Rocco Pizzarelli, Maria Rosito, Maria Giubettini, Tiziana Santini, Cristina Limatola, Giancarlo Ruocco, Davide Ragozzino, Silvia Di Angelantonio
FRONTIERS IN NEUROSCIENCE
(2019)
Article
Oncology
Cristina Bellisai, Ilaria Sciamanna, Paola Rovella, Daniela Giovannini, Mirko Baranzini, Giusj Monia Pugliese, Mohammad Salik Zeya Ansari, Ciro Milite, Paola Sinibaldi-Vallebona, Roberto Cirilli, Gianluca Sbardella, Pietro Pichierri, Daniela Trisciuoglio, Patrizia Lavia, Annalucia Serafino, Corrado Spadafora
Article
Multidisciplinary Sciences
Marialaura Petroni, Maria Sahun Roncero, Valentina Ramponi, Francesca Fabretti, Vittoria Nicolis Di Robilant, Marta Moretti, Vincenzo Alfano, Alessandro Corsi, Simone De Panfilis, Maria Giubettini, Stefano Di Giulio, Carlo Capalbo, Francesca Belardinilli, Anna Coppa, Francesca Sardina, Valeria Colicchia, Flaminia Pedretti, Paola Infante, Beatrice Cardinali, Alessandra Tessitore, Gianluca Canettieri, Enrico De Smaele, Giuseppe Giannini
SCIENTIFIC REPORTS
(2019)
Review
Cell Biology
Francesca Degrassi, Michela Damizia, Patrizia Lavia
Article
Cell Biology
Francesco D. Naso, Valentina Sterbini, Elena Crecca, Italia A. Asteriti, Alessandra D. Russo, Maria Giubettini, Enrico Cundari, Catherine Lindon, Alessandro Rosa, Giulia Guarguaglini
Article
Biochemistry & Molecular Biology
Federica Polverino, Francesco D. Naso, Italia A. Asteriti, Valentina Palmerini, Divya Singh, Davide Valente, Alexander W. Bird, Alessandro Rosa, Marina Mapelli, Giulia Guarguaglini
Summary: Mitotic spindle orientation is a crucial process in cell division, with Aurora-A and TPX2 playing key roles in regulating NuMA localization and spindle alignment. The interaction between TPX2 and Aurora-A is necessary for proper spindle orientation, and both Aurora-A and TPX2 levels can impact the stability of microtubules and spindle orientation.
Article
Genetics & Heredity
Chiara Merigliano, Romina Burla, Mattia La Torre, Simona Del Giudice, Hsiangling Teo, Chong Wai Liew, Alexandre Chojnowski, Wah Ing Goh, Yolanda Olmos, Klizia Maccaroni, Maria Giubettini, Irene Chiolo, Jeremy G. Carlton, Domenico Raimondo, Fiammetta Verni, Colin L. Stewart, Daniela Rhodes, Graham D. Wright, Brian E. Burke, Isabella Saggio
Summary: The study reveals that AKTIP is a component of the ESCRT machinery, similar to TSG101, and plays an important role in cell division. AKTIP physically interacts with other members of the ESCRT machinery, forming a characteristic circular structure, and reducing AKTIP levels leads to defects in ESCRT machinery assembly and cell division.
Article
Cell Biology
Maria Di Bari, Vanessa Tombolillo, Francesco Alessandrini, Claudia Guerriero, Mario Fiore, Italia Anna Asteriti, Emilia Castigli, Miriam Sciaccaluga, Giulia Guarguaglini, Francesca Degrassi, Ada Maria Tata
Summary: The study reveals that the M2 agonist increases aberrant mitosis in glioblastoma cell lines, suggesting it may be a promising novel therapeutic target for glioblastoma treatment.
Article
Neurosciences
Bernadette Basilico, Laura Ferrucci, Patrizia Ratano, Maria T. Golia, Alfonso Grimaldi, Maria Rosito, Valentina Ferretti, Ingrid Reverte, Caterina Sanchini, Maria C. Marrone, Maria Giubettini, Valeria De Turris, Debora Salerno, Stefano Garofalo, Marie-Kim St-Pierre, Micael Carrier, Massimiliano Renzi, Francesca Pagani, Brijesh Modi, Marcello Raspa, Ferdinando Scavizzi, Cornelius T. Gross, Silvia Marinelli, Marie-Eve Tremblay, Daniele Caprioli, Laura Maggi, Cristina Limatola, Silvia Di Angelantonio, Davide Ragozzino
Summary: The study reveals that microglia play an important role in regulating synaptic functioning in the adult brain, and their removal leads to reversible changes in the organization and activity of glutamatergic synapses.
Article
Biochemistry & Molecular Biology
Francesca Rossi, Manuel Beltran, Michela Damizia, Chiara Grelloni, Alessio Colantoni, Adriano Setti, Gaia Di Timoteo, Dario Dattilo, Alvaro Centron-Broco, Carmine Nicoletti, Maurizio Fanciulli, Patrizia Lavia, Irene Bozzoni
Summary: The study reveals that circular RNA circZNF609 can directly interact with multiple mRNAs, increasing their stability and/or translation by recruiting the RNA-binding protein ELAVL1. The interaction with CKAP5 mRNA in particular regulates microtubule function and cell-cycle progression in cancer cells. Additionally, the downregulation of circZNF609 increases the sensitivity of cancer cell lines to different microtubule-targeting chemotherapeutic drugs.
Article
Cell Biology
Federica Cordella, Caterina Sanchini, Maria Rosito, Laura Ferrucci, Natalia Pediconi, Barbara Cortese, Francesca Guerrieri, Giuseppe Rubens Pascucci, Fabrizio Antonangeli, Giovanna Peruzzi, Maria Giubettini, Bernadette Basilico, Francesca Pagani, Alfonso Grimaldi, Giuseppina D'Alessandro, Cristina Limatola, Davide Ragozzino, Silvia Di Angelantonio
Summary: Antibiotic-induced dysbiosis in the adult gut microbiota can lead to altered microglial density and neuronal function in the hippocampus, impairing synaptic efficacy without affecting dendritic spine density. This effect is mediated through the CX3CL1/CX3CR1 signaling pathway, highlighting the importance of microglia-neuron crosstalk in the gut-brain axis.
Article
Biology
Ahmed Abdelbaki, Camilla Ascanelli, Cynthia N. Okoye, H. Begum Akman, Giacomo Janson, Mingwei Min, Chiara Marcozzi, Anja Hagting, Rhys Grant, Maria De Luca, Italia Anna Asteriti, Giulia Guarguaglini, Alessandro Paiardini, Catherine Lindon
Summary: This research investigates the degradation mechanism of the mitotic kinase AURKA. The study finds that the C-terminal D-box of AURKA does not act as a degron but instead mediates essential structural features of the protein. It also shows that the N-terminal intrinsically disordered region of AURKA containing the A-box is sufficient for FZR1-dependent mitotic degradation. Additionally, the study suggests that the QRVL short linear interacting motif in the A-box may be a phospho-regulated D-box.
LIFE SCIENCE ALLIANCE
(2022)
Article
Biology
Italia Anna Asteriti, Federica Polverino, Venturina Stagni, Valentina Sterbini, Camilla Ascanelli, Francesco Davide Naso, Anna Mastrangelo, Alessandro Rosa, Alessandro Paiardini, Catherine Lindon, Giulia Guarguaglini
Summary: AurkA kinase is frequently overexpressed in tumors and its nuclear localization in interphase is correlated with its oncogenic potential. The mechanisms leading to AurkA nuclear accumulation are poorly explored, but it is influenced by the cell cycle phase and nuclear export, and not by its kinase activity. Co-overexpression of AURKA and TPX2 or impairment of proteasome activity can induce AurkA nuclear accumulation.
LIFE SCIENCE ALLIANCE
(2023)