期刊
NUTRITION
卷 28, 期 6, 页码 678-685出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.nut.2011.09.011
关键词
Cryptosporidium parvum; Diarrhea; Malnutrition; Arginine; Growing mice
资金
- National Institutes of Health (NIH) [U54 AI57168]
- National Institute of Allergy and Infectious Diseases
- Fogarty International Center Global Infectious Diseases Research Training program at the NIH [D43 TW006578]
- NIH [5R01HD053131]
- Eunice Kennedy Shriver National Institute of Child Health and Human Development
- NIH Office of Dietary Supplements
Objective: This study investigated the role of L-arginine supplementation to undernourished and Cryptosporidium parvum-infected suckling mice. Methods: The following regimens were initiated on the fourth day of life and injected subcutaneously daily. The C. parvum-infected controls received L-arginine (200 mmol/L) or phosphate buffered saline. The L-arginine-treated mice were grouped to receive NG-nitro-arginine methyl ester CL-NAME) (20 mmol/L) or phosphate buffered saline. The infected mice received orally 106 excysted C. parvum oocysts on day 6 and were euthanized on day 14 at the infection peak. Results: L-arginine improved weight gain compared with the untreated infected controls. L-NAME profoundly impaired body weight gain compared with all other groups. Cryptosporidiosis was associated with ileal crypt hyperplasia, villus blunting, and inflammation. L-arginine improved mucosal histology after the infection. L-NAME abrogated these arginine-induced improvements. The infected control mice showed an intense arginase expression, which was even greater with L-NAME. L-arginine decreased the parasite burden, an effect that was reversed by L-NAME. Cryptosporidium parvum infection increased urine NO3-/NO2- concentrations compared with the uninfected controls, which was increased by L-arginine supplementation, an effect that was also reversed by L-NAME. Conclusion: These findings show a protective role of L-arginine during C parvum infection in undernourished mice, with involvement of arginase I and nitric oxide synthase enzymatic actions. (C) 2012 Elsevier Inc. All rights reserved.
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