Review
Biochemistry & Molecular Biology
Giusi Alberti, Eleonora Russo, Simona Corrao, Rita Anzalone, Peter Kruzliak, Vitale Miceli, Pier Giulio Conaldi, Francesca Di Gaudio, Giampiero La Rocca
Summary: Extracellular vesicles (EVs) are one of the main mechanisms of communication between cells and the surrounding tissue or at distance. Recent studies have revealed the molecular composition and clinical indications of EVs derived from adult mesenchymal stromal cells (MSCs). These EVs maintain some of the properties of their parent cells and have important implications for cell-free therapies.
Review
Cell Biology
Yuan Liu, Christina Holmes
Summary: This review explores the tissue regeneration potential of EVs isolated from bone marrow and adipose tissue, the two most common clinical sources of adult MSCs, focusing on their angiogenic, osteogenic, and immunomodulatory potentials. Other important issues in the development of MSC-derived EV based therapies are also discussed.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell & Tissue Engineering
Xiaoru Xu, Shuang Song, Xiangdong Liu, Yuchao Zhou, Shaojie Shi, Guoqiang Zhao, Xingxing Wang, Xutao Chen, Wenshuang Zhao, Sijia Zhang, Wei Ma, Yingliang Song
Summary: Cell sheet technology utilizing adipose-derived stromal cells (ASCs) shows promise in promoting angiogenesis and may have valuable clinical applications.
STEM CELLS INTERNATIONAL
(2022)
Article
Multidisciplinary Sciences
Hyun-Ji Park, Jessica R. Hoffman, Milton E. Brown, Sruti Bheri, Olga Brazhkina, Young Hoon Son, Michael E. Davis
Summary: Small extracellular vesicles (sEVs) play a critical role in cardiac cell therapy by delivering molecular cargo and mediating cellular signaling. Knocking down miR-192-5p and miR-432-5p in cardiac c-kit+ cell-derived sEVs enhances their therapeutic capabilities and improves cardiac function, reducing fibrosis and necrotic inflammatory responses. This study suggests that targeting deleterious miRNAs in sEVs could be a promising therapeutic strategy for chronic myocardial infarction.
Article
Cell & Tissue Engineering
Shuang Zhang, Jeroen van de Peppel, Marijke Koedam, Johannes P. T. M. van Leeuwen, Bram C. J. van der Eerden
Summary: The study identifies HSPB7 as a regulator of osteogenic and adipogenic differentiation in bone marrow-derived mesenchymal stromal cells (BMSCs), with a positive role in osteoblastic differentiation and maintaining the balance between osteogenesis and adipogenesis. HSPB7 shows potential as a therapeutic target for bone metabolic diseases.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Biology
Zheting Liao, Yu Jin, Yuhao Chu, Hansen Wu, Xiaoyu Li, Zhonghao Deng, Shuhao Feng, Nachun Chen, Ziheng Luo, Xiaoyong Zheng, Liangxiao Bao, Yongqing Xu, Hongbo Tan, Liang Zhao
Summary: A study using single-cell RNA sequencing explored the transcriptomic landscape of alcohol-induced osteonecrosis of the femoral head (ONFH). The study revealed the relevance of stromal and endothelial cells to the pathogenesis of the disease and provided resources for developing cell type-specific therapeutic strategies.
COMMUNICATIONS BIOLOGY
(2022)
Article
Oncology
Rafaela Rossetti, Felipe Augusto Ros, Lucas Eduardo Botelho de Souza, Juliana de Matos Maconetto, Pericles Natan Mendes da Costa, Fernanda Ursoli Ferreira, Josiane Serrano Borges, Julianne Vargas de Carvalho, Nayara Patricia Morotti, Simone Kashima, Dimas Tadeu Covas
Summary: The bone marrow microenvironment may contain two distinct populations of mesenchymal stromal cells (MSC), one located perivascularly and the other in the endosteum. Recent research has focused on better understanding these MSC subsets in order to improve their clinical applications. Studies indicate that compact bone is a rich source of MSC and offers lower risk of contamination by hematopoietic cells. Compact bone-derived MSC also show higher osteogenic potential compared to bone marrow-derived MSC. These findings suggest the presence of functionally different MSC populations in bone marrow and compact bone.
EXPERIMENTAL CELL RESEARCH
(2021)
Review
Oncology
Bushu Harna, Pulkit Kalra, Shivali Arya, Naveen Jeyaraman, Arulkumar Nallakumarasamy, Madhan Jeyaraman, Ramya Lakshmi Rajendran, Eun Jung Oh, Manish Khanna, Uma Maheswari Rajendran, Ho Yun Chung, Byeong-Cheol Ahn, Prakash Gangadaran
Summary: The management of relapses and refractory rheumatoid arthritis (RA) patients is complex and difficult. There are serious side effects and drug interactions with disease-modifying anti-rheumatic drugs (DMARDs), and some subsets of RA patients do not respond to DMARDs. Mesenchymal stem cells (MSCs) provide a promising alternative for RA treatment, with the potential to manage refractory cases not responding to conventional treatment.
EXPERIMENTAL CELL RESEARCH
(2023)
Review
Biochemistry & Molecular Biology
Yujie Song, Christian Jorgensen
Summary: This review highlights the potential of mesenchymal stromal cells (MSCs) in treating osteoarthritis (OA), focusing on their chondrogenic and immunomodulatory potential. The review provides an overview of the effects of MSCs in preclinical and clinical studies of OA treatment, comparing different MSC sources. Furthermore, the review summarizes the current evidence for the assessment of cartilage in OA using magnetic resonance imaging (MRI) and second-look arthroscopy after MSC therapy.
Article
Biotechnology & Applied Microbiology
Pascal Fichtel, Malte von Bonin, Robert Kuhnert, Kristin Moebus, Martin Bornhaeuser, Manja Wobus
Summary: Aging of the hematopoietic system is characterized by an expansion of hematopoietic stem and progenitor cells (HSPCs) with reduced capacity for engraftment, self-renewal, and lymphoid differentiation, resulting in myeloid-biased hematopoiesis.
The communication between HSPCs and MSCs is mediated by direct cell-cell contacts or extracellular vesicles (EVs) that carry bioactive substances. Aging MSCs secrete fewer miRNAs, but aged MSC-EVs contain higher levels of specific miRNAs such as miR-29a and miR-34a. Young EVs enhance the cell number and viability of HSPCs, while aged MSC-EVs promote the differentiation of HSPCs into erythroid and erythroid-megakaryocytic progenitor cells. The therapeutic relevance of MSC-derived EVs in modulating HSPC characteristics needs further investigation.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Cell Biology
Wu Duan, Huajie Zou, Nan Zang, Dongxia Ma, Bo Yang, Lin Zhu
Summary: Bone marrow adipose tissue (MAT) can have both local and systemic effects on metabolic homeostasis. The effects of metformin, a first-line drug for type 2 diabetes, on MAT and bone marrow mesenchymal stem cell (BM-MSC) differentiation are conflicting. In vivo and in vitro experiments showed that metformin increased MAT in mice tibia and promoted both osteogenic and lipogenic gene expression in mouse bone marrow. However, metformin inhibited lipogenesis and promoted osteogenesis of MSC in vitro. The increased MAT may be attributed to the filling of adipose tissue after metformin-induced apoptosis of bone marrow stromal cells.
Article
Oncology
Amanpreet Kaur Bains, Lena Behrens Wu, Jennifer Riviere, Sandra Rother, Valentina Magno, Jens Friedrichs, Carsten Werner, Martin Bornhaeuser, Katharina S. Goetze, Michael Cross, Uwe Platzbecker, Manja Wobus
Summary: Myelodysplastic syndromes (MDS) are a heterogeneous group of blood malignancies with specific features and a high risk of progression to acute myeloid leukemia. This study found that MSC-derived extracellular matrix (ECM) plays an important role in MDS, with structural alterations observed in MDS ECM and a potential involvement of glycosaminoglycans (GAGs) in this process.
FRONTIERS IN ONCOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Yi-Xuan Wang, Zhu-Li Peng, Zhi-Wen Sun, Yan-Jun Pan, Hong Ai, Zhi-Hui Mai
Summary: This study investigates the role of miR-20 in promoting osteogenic differentiation in BMSCs and accelerating bone formation in a rabbit maxillary sinus bone defect model. Overexpression of miR-20a increased the levels of osteogenic markers and enhanced calcified nodule deposition in BMSCs, while anti-miR-20a treatment had the opposite effect. In the rabbit model, miR-20a delivery significantly improved bone density, osteoblast numbers, and collagen type 1 secretion compared to Bio-Oss bone powder.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Cell & Tissue Engineering
Stefano Negri, Yiyun Wang, Takashi Sono, Qizhi Qin, Ginny Ching-Yun Hsu, Masnsen Cherief, Jiajia Xu, Seungyong Lee, Robert J. Tower, Victoria Yu, Abhi Piplani, Carolyn A. Meyers, Kristen Broderick, Min Lee, Aaron W. James
Summary: Research has shown that anti-DKK1 neutralizing antibodies can enhance the osteogenic differentiation of human adipose-derived stem cells, aiding in bone repair, and demonstrating potential beneficial effects in a mouse model.
STEM CELLS TRANSLATIONAL MEDICINE
(2021)
Article
Cell & Tissue Engineering
Behnoosh Tayebi, Mahnaz Babaahmadi, Mohammad Pakzad, Mostafa Hajinasrollah, Farhad Mostafaei, Shahrbanoo Jahangiri, Amir Kamali, Hossein Baharvand, Mohamadreza Baghaban Eslaminejad, Seyedeh-Nafiseh Hassani, Ensiyeh Hajizadeh-Saffar
Summary: Mesenchymal stromal cells (MSCs) have shown potential in treating inflammatory and non-inflammatory diseases, but their clinical applications require strict evaluations and monitoring to ensure compliance with safety standards. This study aimed to assess the potential toxicity effects of cryopreserved human bone marrow-derived clonal MSCs in animal models during different time periods.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Cell & Tissue Engineering
Jacques Galipeau, Mauro Krampera, Katarina Leblanc, Jan A. Nolta, Donald G. Phinney, Yufang Shi, Karin Tarte, Sowmya Viswanathan, Ivan Martin
Summary: The authors highlight the significant impact of in vivo persistence on the potency of MSCs, which may not be predicted by in vitro cellular potency assays and its translation to in vivo outcomes. Additionally, it is suggested that MSC viability and metabolic fitness play a dominant role in potency quality attributes, particularly in recipients identified by emerging predictive biomarkers of response.
Article
Hematology
Erwan Dumontet, Celine Pangault, David Roulois, Matthis Desoteux, Simon Leonard, Tony Marchand, Maelle Latour, Patricia Legoix, Damarys Loew, Florent Dingli, Joelle Dulong, Erwan Flecher, Cedric Coulouarn, Guillaume Cartron, Thierry Fest, Karin Tarte
Summary: Follicular lymphoma-derived extracellular vesicles (EVs) can enhance the support of FL B-cell growth by BM-MSCs, alter gene expression, activate different signaling pathways, and potentially contribute to the formation of a permissive stromal niche in the bone marrow for FL.
Article
Cell Biology
Mathieu Lesouhaitier, Murielle Gregoire, Arnaud Gacouin, Valentin Coirier, Aurelien Frerou, Caroline Piau, Vincent Cattoir, Erwan Dumontet, Matthieu Revest, Pierre Tattevin, Antoine Roisne, Jean-Philippe Verhoye, Erwan Flecher, Yves Le Tulzo, Karin Tarte, Jean-Marc Tadie
Summary: This study aimed to investigate the impact of cardiopulmonary bypass (CPB) on neutrophil functions. The results showed that CPB induced a systemic inflammation with an increase in circulating mature neutrophils after surgery. Neutrophil lifespan was significantly increased and functions such as NET release and ROS production were enhanced after CPB, while bacteria killing and phagocytosis were not impacted. These data suggest that CPB recruits mature neutrophils via a demargination process and neutrophils remain efficient post-CPB without contributing to immunosuppression.
JOURNAL OF LEUKOCYTE BIOLOGY
(2022)
Article
Hematology
Marion Haas, Gersende Caron, Fabrice Chatonnet, Stephane Manenti, Elina Alaterre, Julie Devin, Celine Delaloy, Giulia Bertolin, Roselyne Viel, Amandine Pignarre, Francisco Llamas-Gutierrez, Anne Marchalot, Olivier Decaux, Karin Tarte, Laurent Delpy, Jerome Moreaux, Thierry Fest
Summary: The kinase PIM2 plays a crucial role in the differentiation of B cells and in the survival of mature normal and malignant plasma cells. PIM2 regulates the cell cycle and apoptotic processes, affecting the secretion ability and adaptability of plasma cells.
Article
Biochemistry & Molecular Biology
Pierre-Jean Ferron, Brendan Le Dare, Julie Bronsard, Clara Steichen, Elodie Babina, Romain Pelletier, Thierry Hauet, Isabelle Morel, Karin Tarte, Florian Reizine, Bruno Clement, Bernard Fromenty, Thomas Gicquel
Summary: The study investigated the effects of using hydroxychloroquine (HCQ) to treat COVID-19 patients, especially obese patients, and its impact on drug metabolism and hepatotoxicity. In vitro experiments on HepaRG and RPTEC cells showed that HCQ metabolism was altered in conditions of hepatic steatosis in HepaRG cells, leading to the detection of a new metabolite (carboxychloroquine). Furthermore, HepaRG cells exposed to exogenous fatty acids exhibited increased cytotoxicity of HCQ and enhanced neutral lipid accumulation. These in vitro findings were compared with clinical data from COVID-19 patients treated with HCQ, suggesting that steatosis may increase the risk of altered drug metabolism and potential toxicity of HCQ.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Nanoscience & Nanotechnology
Clement Goubault, Ulrich Jarry, Megane Bostoen, Pierre-Antoine Eliat, Myrtil L. Kahn, Remy Pedeux, Thierry Guillaudeux, Fabienne Gauffre, Soizic Chevance
Summary: This study evaluated the potentiating effect of hollow shells made of gold and iron oxide nanoparticles on radiotherapy for glioblastoma. The results showed a significant effect of gold nanoparticles in radiotherapy, while iron oxide had no beneficial effects. The study also found that injected hybridosomes(R) remained near the brain tumor for several days in vivo. These findings provide a proof-of-concept for the effectiveness of hybridosomes(R) as tools for the development of combined therapies.
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
(2022)
Article
Multidisciplinary Sciences
Florian Reizine, Murielle Gregoire, Mathieu Lesouhaitier, Valentin Coirier, Juliette Gauthier, Celine Delaloy, Elise Dessauge, Florent Creusat, Fabrice Uhel, Arnaud Gacouin, Frederic Dessauge, Cecile Le Naoures, Caroline Moreau, Claude Bendavid, Yoann Daniel, Kilian Petitjean, Valerie Bordeau, Claire Lamaison, Caroline Piau, Vincent Cattoir, Mikael Roussel, Bernard Fromenty, Christian Michelet, Yves Le Tulzo, Jaroslaw Zmijewski, Ronan Thibault, Michel Cogne, Karin Tarte, Jean-Marc Tadie
Summary: Severe sepsis can lead to sustained immune dysfunction, resulting in organ failure, secondary infections, and late mortality. Supplementation with arginine or citrulline can restore T cell function and reduce immunosuppression induced by sepsis.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Immunology
Kathleen Santamaria, Fabienne Desmots, Simon Leonard, Gersende Caron, Marion Haas, Celine Delaloy, Fabrice Chatonnet, Delphine Rossille, Amandine Pignarre, Celine Monvoisin, Marine Seffals, Claire Lamaison, Michel Cogne, Karin Tarte, Thierry Fest
Summary: The expression of CD23 marker plays a crucial role in the differentiation of human LZ B-GC cells, as CD23 expression is restricted to the GC LZ. CD23-negative LZ B-GC cells can differentiate into plasmablasts, while cells expressing CD23 are unable to complete this differentiation process. Analysis indicates that the process of plasma cell differentiation involves a series of gene expression changes, including a transient MYC gene expression supporting B cell proliferation.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biology
Alexandra Papaioannou, Federica Centonze, Alice Metais, Marion Maurel, Luc Negroni, Matias Gonzalez-Quiroz, Sayyed Jalil Mahdizadeh, Gabriella Svensson, Ensieh Zare, Alice Blondel, Albert C. Koong, Claudio Hetz, Remy Pedeux, Michel L. Tremblay, Leif A. Eriksson, Eric Chevet
Summary: The IRE1 alpha RNase regulatory network is dynamically regulated by tyrosine kinases and phosphatases. The extent of RtcB tyrosine phosphorylation determines cell adaptive or death outputs.
LIFE SCIENCE ALLIANCE
(2022)
Article
Rheumatology
Dominique Farge, Severine Loisel, Matthieu Resche-Rigon, Pauline Lansiaux, Ines Colmegna, David Langlais, Catney Charles, Gregory Pugnet, Alexandre Thibault Jacques Maria, Emmanuel Chatelus, Thierry Martin, Eric Hachulla, Vissal David Kheav, Nathalie C. Lambert, Han Chen Wang, David Michonneau, Christophe Martinaud, Luc Sensebe, Audrey Crast, Karin Tarte
Summary: This study aimed to evaluate the safety and feasibility of mesenchymal stromal cell therapy derived from bone marrow to treat severe diffuse systemic sclerosis. The results showed that a single intravenous injection of allogeneic bone marrow-derived mesenchymal stromal cells was safe and well-tolerated in patients with severe diffuse systemic sclerosis.
LANCET RHEUMATOLOGY
(2022)
Article
Cell & Tissue Engineering
Joelle Dulong, Severine Loisel, Delphine Rossille, Simon Leonard, Nadege Bescher, Isabelle Bezier, Maelle Latour, Celine Monvoisin, Delphine Monnier, Nicolas Bertheuil, David Roulois, Karin Tarte
Summary: This study identified the upregulation of CD40L in adipose tissue and the overexpression of CD40 in ASCs in obese patients. The CD40-CD40L axis plays a crucial role in modulating the interaction between ASCs and neutrophils, highlighting its therapeutic potential.
Article
Oncology
Charly Gutierrez, Xavier Pinson, Kathleen Jarnouen, Marine Charpentier, Raphael Pineau, Laetitia Lallement, Remy Pedeux
Summary: Urinary cytology is a non-invasive and easy-to-collect method for detecting bladder cancer. It is effective for high-grade bladder cancer, but less effective for low-grade cancer. Recent research has found that the fluorescent properties of urothelial tumor cells in urinary cytology, known as peri-membrane fluorescence, can improve early detection of bladder cancer.
Article
Immunology
Lamine Alaoui, Javiera Villar, Renaud Leclere, Simon Le Gallou, Francis Relouzat, Henri-Alexandre Michaud, Karin Tarte, Natacha Teissier, Benoit Favier, Mikael Roussel, Elodie Segura
Summary: This study characterizes the phenotype, transcriptome, life cycle, and function of human tonsil macrophages, and identifies three subtypes with distinct features. CD36(hi) macrophages are related to monocytes, CD36(lo) macrophages have features of embryonic origin, and CD36(int) macrophages display mixed characteristics. Functionally, CD36(hi) macrophages are specialized in stimulating T follicular helper cells by producing Activin A. Stromal cell-derived TNF-alpha is identified as the inducer of Activin A secretion.
JOURNAL OF EXPERIMENTAL MEDICINE
(2023)
Article
Cell & Tissue Engineering
Severine Loisel, Pauline Lansiaux, Delphine Rossille, Cedric Menard, Joelle Dulong, Celine Monvoisin, Nadege Bescher, Isabelle Bezier, Maelle Latour, Audrey Cras, Dominique Farge, Karin Tarte
Summary: Initial overexpression of profibrotic factors by B cells is associated with a lack of clinical response to MSCs in severe SSc patients. Increase of regulatory B cells is a marker of clinical response to MSCs in SSc patients. MSCs directly upregulate IL-10 production by activated B cells in vitro.
STEM CELLS TRANSLATIONAL MEDICINE
(2023)
Article
Hematology
Martin Dreyling, Marc Andre, Nicola Gokbuget, Herve Tilly, Mats Jerkeman, John Gribben, Andres Ferreri, Pierre Morel, Stephan Stilgenbauer, Christopher Fox, Jose Maria Ribera, Sonja Zweegman, Igor Aurer, Csaba Bodor, Birgit Burkhardt, Christian Buske, Maria Dollores Caballero, Elias Campo, Bjoern Chapuy, Andrew Davies, Laurence de Leval, Jeanette Doorduijn, Massimo Federico, Philippe Gaulard, Francesca Gay, Paolo Ghia, Kirsten Gronbaek, Hartmut Goldschmidt, Marie-Jose Kersten, Barbara Kiesewetter, Judith Landman-Parker, Steven Le Gouill, Georg Lenz, Sirpa Leppa, Armando Lopez-Guillermo, Elizabeth Macintyre, Maria Victoria Mateos Mantega, Philippe Moreau, Carol Moreno, Bertrand Nadel, Jessica Okosun, Roger Owen, Sarka Pospisilova, Christiane Pott, Tadeusz Robak, Michelle Spina, Kostas Stamatopoulos, Jan Stary, Karin Tarte, Allessandra Tedeschi, Catherine Thieblemont, Ralf Ulrich Trappe, Lorenz H. Trumper, Gilles Salles