Article
Biochemical Research Methods
Florent Velay, Melanie Soula, Marwa Mehrez, Clement Belbachir, Stefano D'Alessandro, Christophe Laloi, Patrice Crete, Ben Field
Summary: This study describes the development of a flexible modular cloning-based toolkit for bimolecular fluorescence complementation (BiFC) and proximity labelling in the chloroplast and other cellular compartments. The toolkit simplifies the construction of chloroplast fusion proteins, enables robust ratiometric quantification, and provides model positive and negative controls. The study highlights potential pitfalls in designing BiFC experiments, such as the choice of FP split, negative controls, cell type, and reference FP.
Article
Biochemistry & Molecular Biology
Ozge Ugurlu, Serap Evran
Summary: YopM, an essential effector protein of Yersinia, shows immunosuppressive effect through protein-protein interactions in host cells. This study investigated the protein-protein interactions of YopM with T3SS components LcrV and LcrG, and found that YopM interacts with LcrG. BiFC assay is proposed as a simple method to screen novel interaction partners of YopM.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Review
Geriatrics & Gerontology
Jiang Chen, Jun-Sheng Chen, Song Li, Fengning Zhang, Jie Deng, Ling-Hui Zeng, Jun Tan
Summary: Decades of research have shown that amyloid-beta (Aβ) plays an undeniable role in the development of Alzheimer's disease (AD). However, the focus on the pathological effects of Aβ may overshadow the significance of its metabolic precursor, amyloid precursor protein (APP), in the occurrence and progression of AD. This review explores the various roles of APP in AD, including its structure, functions, enzymatic processing, and potential therapeutic approaches to targeting APP to ameliorate AD pathologies and halt disease progression.
Review
Biochemistry & Molecular Biology
Kseniia S. Orobets, Andrey L. Karamyshev
Summary: Alzheimer's disease is a common neurodegenerative disorder associated with age or inherited mutations. It is characterized by severe dementia that affects memory, cognitive functions, and daily life. The disease is linked to the accumulation of cytotoxic amyloid beta and hyperphosphorylated tau protein, as well as other pathological features. Various treatment options, such as antibody-based therapy and stem cell transplantation, are being investigated.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Analytical
Ui Jin Lee, Yunkwang Oh, Oh Seok Kwon, Yong-Beom Shin, Moonil Kim
Summary: We developed a highly sensitive and specific bimolecular fluorescence complementation (BiFC)-based influenza A virus (IAV)-sensing system. The system utilizes a galactose/glucose-binding protein (GGBP) with a N-terminal large domain (YN1-172) and a C-terminal small domain (YC173-239) made up of enhanced yellow fluorescence protein (eYFP). The system demonstrated ultra-high sensitivity and selective responses for IAV detection, making it a simple and effective platform for IAV detection without the need for virus culture or RNA extraction processes.
Article
Plant Sciences
Timothee Laloux, Irwin Matyjaszczyk, Simon Beaudelot, Charles Hachez, Francois Chaumont
Summary: It was found that the interaction between SYP121 and PIP2;7 depends on specific structural regions, rather than individual Habc or Qa-SNARE domains. Deletion of the N- and/or C-terminus of the PIP2;7 protein leads to a decrease in interaction with SYP121. This suggests that the interaction motifs in both SYP121 and PIP2;7 depend on the protein conformation.
FRONTIERS IN PLANT SCIENCE
(2021)
Review
Neurosciences
Rebecca M. C. Gabriele, Emily Abel, Nick C. Fox, Selina Wray, Charles Arber
Summary: Amyloid precursor protein (APP) and its cleavage fragment Amyloid-beta (A beta) play crucial roles in Alzheimer's disease (AD). Genetic alterations that increase the overall dosage of APP or favor the generation of more aggregation-prone A beta species directly contribute to the disease. Lowering APP expression is an attractive approach for AD treatment and prevention. New technologies that reduce APP expression may offer disease modification and slow clinical progression.
FRONTIERS IN NEUROSCIENCE
(2022)
Article
Geriatrics & Gerontology
Jinsu Park, Meenu Madan, Srinivasulu Chigurupati, Seung Hyun Baek, Yoonsuk Cho, Mohamed R. Mughal, Amin Yu, Sic L. Chan, Jogi Pattisapu, Mark P. Mattson, Dong-Gyu Jo
Summary: AQP1 levels are elevated in the cerebral cortex during the early stages of AD, particularly in vulnerable neurons, and increase as aging progresses in AD mouse models. AQP1 appears to reduce A beta production by inhibiting the binding between BACE1 and APP.
JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES
(2021)
Article
Biology
Soyeon Lee, Dongkeun Park, Chunghun Lim, Jae-Ick Kim, Kyung-Tai Min
Summary: Mitochondria play a crucial role in the distribution and localization of energy in neurites, with recent studies highlighting the importance of local mRNA translation in axons. The research demonstrates that brain-derived neurotrophic factor (BDNF) induces local translation of mitochondrial initiation factor 3 (mtIF3) mRNA in axonal growth cones, leading to promotion of mitochondrial translation and maintenance of axonal growth. These findings provide new insights into the adaptive control of mitochondrial physiology and axonal development through local mtIF3 translation.
Review
Biotechnology & Applied Microbiology
Chih Hung Lo
Summary: Tau protein plays a crucial role in physiological functions but is also implicated in Alzheimer's disease and tauopathies. Recent studies have highlighted soluble tau oligomers as the primary toxic species. The use of cellular biosensor technology has advanced our understanding of tau oligomerization and aggregation, providing insights into tau conformational ensembles and propagation of pathology.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Xiaoling Liu, Yan Liu, Shangrong Ji
Summary: Alzheimer's disease is a common neurodegenerative disease that increases in prevalence with age. Studies suggest that abnormalities in the metabolism of APP are a major pathological feature of AD, with secretases playing a key role in APP processing. Research on secretases in the processing of APP could potentially lead to new directions for AD therapy.
Review
Pharmacology & Pharmacy
Yoonsuk Cho, Han-Gyu Bae, Eitan Okun, Thiruma V. Arumugam, Dong-Gyu Jo
Summary: APP is an evolutionarily conserved transmembrane protein that serves as a precursor to amyloid-beta peptides, which are implicated in Alzheimer's disease. Studies have shown diverse pathological and physiological functions of APP and its cleavage products, but their roles are not fully understood. Current research focuses on APP processing and potential therapeutic approaches for Alzheimer's disease.
PHARMACOLOGY & THERAPEUTICS
(2022)
Review
Biochemistry & Molecular Biology
Nicolas Papadopoulos, Nuria Suelves, Florian Perrin, Devkee M. Vadukul, Celine Vrancx, Stefan N. Constantinescu, Pascal Kienlen-Campard
Summary: Most neurodegenerative diseases are characterized by protein folding disorders, such as Alzheimer's disease. These diseases lead to the appearance of protein aggregates in vulnerable regions of the nervous system, which progressively spread through the neuronal network. Alzheimer's disease is characterized by neurofibrillary tangles composed of tau proteins and senile plaques composed of amyloid peptides. Understanding the structural determinants of the precursor protein APP and the formation of different A beta aggregates is crucial in deciphering the pathological conformational changes and mechanisms underlying amyloid fibril formation.
Article
Plant Sciences
Yuanyuan Jiang, Jiangrong Peng, Yunpeng Cao, Zhiqiang Han, Ling Zhang, Wenbing Su, Shunquan Lin, Yuan Yuan, Bin Wang, Xianghui Yang, Zhike Zhang
Summary: The study introduced a new method for observing the cell structure of Nicotiana benthamiana, significantly improving imaging by fluorescence microscopy and allowing clear visualization of cellular structures and protein interactions under high-power lens. This method provides insights and references for the study of cell structures and functions in other plants, demonstrating its applicability in live cell visualization through confocal scanning laser microscopy.
PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS
(2021)
Article
Geriatrics & Gerontology
Kenji Yamamoto, Ryo Yamamoto, Nobuo Kato
Summary: In this study, it was found that specific antibodies can recover the activity of BK channels suppressed by Aβ oligomers in neurons of Alzheimer's disease model mice, suggesting an important role of APP in this process.
FRONTIERS IN AGING NEUROSCIENCE
(2021)
Article
Multidisciplinary Sciences
Astrid B. Van Belle, Perrine M. Cochez, Magali de Heusch, Lisa Pointner, Remi Opsomer, Peggy Raynaud, Younes Achouri, Emilie Hendrickx, Pamela Cheou, Guy Warnier, Jean-Christophe Renauld, Marie Baeck, Laure Dumoutier
SCIENTIFIC REPORTS
(2019)
Article
Immunology
Magali de Heusch, Valerie Steenwinckel, Perrine M. Cochez, Jamila Louahed, Guy Warnier, Muriel M. Lemaire, Jean-Christophe Renauld, Laure Dumoutier
EUROPEAN JOURNAL OF IMMUNOLOGY
(2020)
Article
Oncology
Delia Hoffmann, Tereza Dvorakova, Vincent Stroobant, Caroline Bouzin, Aurelie Daumerie, Marie Solvay, Simon Klaessens, Marie-Claire Letellier, Jean-Christophe Renauld, Nicolas van Baren, Julie Lelotte, Etienne Marbaix, Benoit J. Van den Eynde
CANCER IMMUNOLOGY RESEARCH
(2020)
Article
Biotechnology & Applied Microbiology
Bram Laukens, Pieter P. Jacobs, Katelijne Geysens, Jose Martins, Charlot De Wachter, Paul Ameloot, Willy Morelle, Jurgen Haustraete, Jean-Christophe Renauld, Bart Samyn, Roland Contreras, Simon Devos, Nico Callewaert
BIOTECHNOLOGY AND BIOENGINEERING
(2020)
Article
Medicine, Research & Experimental
Ylia Salazar, Xiang Zheng, David Brunn, Hartmann Raifer, Felix Picard, Yajuan Zhang, Hauke Winter, Stefan Guenther, Andreas Weigert, Benno Weigmann, Laure Dumoutier, Jean-Christophe Renauld, Ari Waisman, Anja Schmall, Amanda Tufman, Ludger Fink, Bernhard Bruene, Tobias Bopp, Friedrich Grimminger, Werner Seeger, Soni Savai Pullamsetti, Magdalena Huber, Rajkumar Savai
JOURNAL OF CLINICAL INVESTIGATION
(2020)
Review
Biochemistry & Molecular Biology
Serena Stanga, Marina Boido, Pascal Kienlen-Campard
Summary: The neuromuscular junction (NMJ) is a crucial interface between the nervous system and muscles, where the Glial cell line-Derived Neurotrophic Factor (GDNF) plays a key role in synaptic formation, stabilization, and function, as well as in protecting the NMJ during neurodegeneration. GDNF also has unique properties that make it essential in neurodegenerative disorders. Research highlights the importance of GDNF in the NMJ-muscle interaction, and its therapeutic potential in diseases like Alzheimer's, ALS, and SMA. Additionally, 3D neuromuscular co-culture systems are being developed as models to study NMJ formation and involvement in neuromuscular disorders.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Neurosciences
Sabrina Contino, Nuria Suelves, Celine Vrancx, Devkee M. Vadukul, Valery L. Payen, Serena Stanga, Luc Bertrand, Pascal Kienlen-Campard
Summary: The study found that the mitochondrial phenotype associated with PS1 and PS2 was not observed in primary cells, suggesting that the results obtained from immortalized cell lines may not be applicable to central nervous system cells. Further analysis of PS2KO primary fibroblasts revealed no mitochondrial dysfunction, consistent with observations in primary neurons and astrocytes. The PS-dependent mitochondrial phenotype reported so far may be a consequence of the cell immortalization process and should be critically reconsidered in relation to Alzheimer's disease.
FRONTIERS IN NEUROSCIENCE
(2021)
Article
Pharmacology & Pharmacy
Cristina Loira-Pastoriza, Kevin Vanvarenberg, Bernard Ucakar, Maria Machado Franco, Aurelie Staub, Muriel Lemaire, Jean-Christophe Renauld, Rita Vanbever
Summary: Immunotherapy using immunostimulatory agents delivered intratumorally has shown promising efficacy in the treatment of lung cancer. Encapsulation of unmethylated oligodeoxynucleotides containing CpG motifs (CpG) and polyinosinic-polycytidylic acid (poly I:C) double-stranded RNA in liposomes can enhance their antitumor activity by increasing uptake by lung phagocytes and activating toll-like receptors. CpG encapsulation in DOTAP/DPPC liposomes significantly enhances its antitumor effects in lung cancer by inducing immune responses and delaying tumor growth.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Neurosciences
Celine Vrancx, Devkee M. Vadukul, Nuria Suelves, Sabrina Contino, Ludovic D'Auria, Florian Perrin, Vincent van Pesch, Bernard Hanseeuw, Loic Quinton, Pascal Kienlen-Campard
Summary: This study reveals the presence of hexameric-like Aβ assemblies in various cellular models, including neuronal-like cell lines. It also investigates the role of presenilin-1 and presenilin-2 in the formation of hexameric Aβ. Furthermore, the study suggests hexameric Aβ as a potential early biomarker for AD and demonstrates its ability to seed other Aβ forms, contributing to amyloid deposition in vivo and neuronal toxicity in vitro.
MOLECULAR NEUROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Mariko Kamioka, Yoshiyuki Goto, Kiminori Nakamura, Yuki Yokoi, Rina Sugimoto, Shuya Ohira, Yosuke Kurashima, Shingo Umemoto, Shintaro Sato, Jun Kunisawa, Yu Takahashi, Steven E. Domino, Jean-Christophe Renauld, Susumu Nakae, Yoichiro Iwakura, Peter B. Ernst, Tokiyoshi Ayabe, Hiroshi Kiyono
Summary: Paneth cells in mice can be differentiated into two subtypes based on their production and utilization of fucosyltransferase 2 (Fut2). Fut2(+) Paneth cells are involved in host defense by creating niches for commensal bacteria and preventing invasion of pathogens.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Immunology
Yongyao Fu, Jocelyn Wang, Baohua Zhou, Abigail Pajulas, Hongyu Gao, Baskar Ramdas, Byunghee Koh, Benjamin J. Ulrich, Shuangshuang Yang, Reuben Kapur, Jean-Christophe Renauld, Sophie Paczesny, Yunlong Liu, Robert M. Tighe, Paula Licona-Limon, Richard A. Flavell, Shogo Takatsuka, Daisuke Kitamura, Robert S. Tepper, Jie Sun, Mark H. Kaplan
Summary: This study reveals that IL-9 mediates proallergic activities in the lungs by targeting lung macrophages. Interstitial macrophages are required for IL-9-dependent allergic responses. Mechanistically, IL-9 affects the function of lung macrophages by inducing Arg1 activity.
SCIENCE IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Yongyao Fu, Abigail Pajulas, Jocelyn Wang, Baohua Zhou, Anthony Cannon, Cherry Cheuk Lam Cheung, Jilu Zhang, Huaxin Zhou, Amanda Jo Fisher, David T. Omstead, Sabrina Khan, Lei Han, Jean-Christophe Renauld, Sophie Paczesny, Hongyu Gao, Yunlong Liu, Lei Yang, Robert M. Tighe, Paula Licona-Limon, Richard A. Flavell, Shogo Takatsuka, Daisuke Kitamura, Jie Sun, Basar Bilgicer, Catherine R. Sears, Kai Yang, Mark H. Kaplan
Summary: The role of IL-9 in the tumor microenvironment and its effects on macrophages have been investigated. The study shows that IL-9 promotes the expansion of pulmonary macrophages and targeting the IL-9R/arginase 1 axis restricts tumor growth, suggesting that this cytokine pathway could be a potential therapeutic target for lung cancer.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Nathalie Kyalu Ngoie Zola, Clemence Balty, Sebastien Pyr dit Ruys, Axelle A. T. Vanparys, Nicolas D. G. Huyghe, Gaetan Herinckx, Manuel Johanns, Emilien Boyer, Pascal Kienlen-Campard, Mark H. Rider, Didier Vertommen, Bernard J. Hanseeuw
Summary: Tau isoforms in insoluble aggregates differ between tauopathies, but not in soluble brain extracts. This study characterizes the post-translational modifications of aggregated and soluble tau protein in various tauopathies, revealing specific soluble signatures for each tauopathy.
NATURE COMMUNICATIONS
(2023)
Correction
Oncology
I Theate, N. van Baren, L. Pilotte, P. Moulin, P. Larrieu, J-C Renauld
CANCER IMMUNOLOGY RESEARCH
(2020)
Article
Physiology
Ann-Christin Gnirck, Malte Wunderlich, Martina Becker, Tingting Xiong, Ella Weinert, Catherine Meyer-Schwesinger, Laure Dumoutier, Jean-Christophe Renauld, Samuel Huber, Ulf Panzer, Jan-Eric Turner
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2019)