Molecular mechanisms of CRISPR–Cas spacer acquisition

Cas4-Dependent Prespacer Processing Ensures High-Fidelity Programming of CRISPR Arrays

(2018) Hayun Lee et al.   MOLECULAR CELL

Cas4 Nucleases Define the PAM, Length, and Orientation of DNA Fragments Integrated at CRISPR Loci

(2018) Masami Shiimori et al.   MOLECULAR CELL

Cas4 Facilitates PAM-Compatible Spacer Selection during CRISPR Adaptation

(2018) Sebastian N. Kieper et al.   Cell Reports

Mutations in Cas9 Enhance the Rate of Acquisition of Viral Spacer Sequences during the CRISPR-Cas Immune Response

(2017) Robert Heler et al.   MOLECULAR CELL

CRISPR–Cas systems exploit viral DNA injection to establish and maintain adaptive immunity

(2017) Joshua W. Modell et al.   NATURE

CRISPR–Cas encoding of a digital movie into the genomes of a population of living bacteria

(2017) Seth L. Shipman et al.   NATURE

How type II CRISPR–Cas establish immunity through Cas1–Cas2-mediated spacer integration

(2017) Yibei Xiao et al.   NATURE

Spacer-length DNA intermediates are associated with Cas1 in cells undergoing primed CRISPR adaptation

(2017) Olga Musharova et al.   NUCLEIC ACIDS RESEARCH

Prespacer processing and specific integration in a Type I-A CRISPR system

(2017) Clare Rollie et al.   NUCLEIC ACIDS RESEARCH

Impact of backbone fluorination on nanoscale morphology and excitonic coupling in polythiophenes

(2017) Zhongjian Hu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structures of the CRISPR genome integration complex

(2017) Addison V. Wright et al.   SCIENCE

Kinetics of dCas9 target search inEscherichia coli

(2017) Daniel Lawson Jones et al.   SCIENCE

Multiplex recording of cellular events over time on CRISPR biological tape

(2017) Ravi U. Sheth et al.   SCIENCE

CRISPR-Cas: Adapting to change

(2017) Simon A. Jackson et al.   SCIENCE

The CRISPR Spacer Space Is Dominated by Sequences from Species-Specific Mobilomes

(2017) Sergey A. Shmakov et al.   mBio

On the Origin of Reverse Transcriptase-Using CRISPR-Cas Systems and Their Hyperdiverse, Enigmatic Spacer Repertoires

(2017) Sukrit Silas et al.   mBio

Bipartite recognition of target RNAs activates DNA cleavage by the Type III-B CRISPR–Cas system

(2016) Joshua R. Elmore et al.   GENES & DEVELOPMENT

Conformational Control of Cascade Interference and Priming Activities in CRISPR Immunity

(2016) Chaoyou Xue et al.   MOLECULAR CELL

Quorum Sensing Controls Adaptive Immunity through the Regulation of Multiple CRISPR-Cas Systems

(2016) Adrian G. Patterson et al.   MOLECULAR CELL

CRISPR Immunological Memory Requires a Host Factor for Specificity

(2016) James K. Nuñez et al.   MOLECULAR CELL

CRISPR-Cas Systems Optimize Their Immune Response by Specifying the Site of Spacer Integration

(2016) Jon McGinn et al.   MOLECULAR CELL

Adaptation in CRISPR-Cas Systems

(2016) Samuel H. Sternberg et al.   MOLECULAR CELL

Cas3-Derived Target DNA Degradation Fragments Fuel Primed CRISPR Adaptation

(2016) Tim Künne et al.   MOLECULAR CELL

CRISPR–Cas adaptation: insights into the mechanism of action

(2016) Gil Amitai et al.   NATURE REVIEWS MICROBIOLOGY

Protecting genome integrity during CRISPR immune adaptation

(2016) Addison V Wright et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Highly efficient primed spacer acquisition from targets destroyed by theEscherichia colitype I-E CRISPR-Cas interfering complex

(2016) Ekaterina Semenova et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Quorum sensing controls thePseudomonas aeruginosaCRISPR-Cas adaptive immune system

(2016) Nina M. Høyland-Kroghsbo et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Molecular recordings by directed CRISPR spacer acquisition

(2016) Seth L. Shipman et al.   SCIENCE

Direct CRISPR spacer acquisition from RNA by a natural reverse transcriptase-Cas1 fusion protein

(2016) S. Silas et al.   SCIENCE

Diverse evolutionary roots and mechanistic variations of the CRISPR-Cas systems

(2016) Prarthana Mohanraju et al.   SCIENCE

Interference-driven spacer acquisition is dominant over naive and primed adaptation in a native CRISPR–Cas system

(2016) Raymond H. J. Staals et al.   Nature Communications

Protecting genome integrity during CRISPR immune adaptation

(2016) Addison V Wright et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Surveillance and Processing of Foreign DNA by the Escherichia coli CRISPR-Cas System

(2015) Sy Redding et al.   CELL

Structural and Mechanistic Basis of PAM-Dependent Spacer Acquisition in CRISPR-Cas Systems

(2015) Jiuyu Wang et al.   CELL

Co-transcriptional DNA and RNA Cleavage during Type III CRISPR-Cas Immunity

(2015) Poulami Samai et al.   CELL

The Contribution of Genetic Recombination to CRISPR Array Evolution

(2015) Anne Kupczok et al.   Genome Biology and Evolution

Two Distinct DNA Binding Modes Guide Dual Roles of a CRISPR-Cas Protein Complex

(2015) Timothy R. Blosser et al.   MOLECULAR CELL

Integrase-mediated spacer acquisition during CRISPR–Cas adaptive immunity

(2015) James K. Nuñez et al.   NATURE

Foreign DNA capture during CRISPR–Cas adaptive immunity

(2015) James K. Nuñez et al.   NATURE

CRISPR adaptation biases explain preference for acquisition of foreign DNA

(2015) Asaf Levy et al.   NATURE

Cas9 specifies functional viral targets during CRISPR–Cas adaptation

(2015) Robert Heler et al.   NATURE

CRISPR interference and priming varies with individual spacer sequences

(2015) Chaoyou Xue et al.   NUCLEIC ACIDS RESEARCH

Sequences spanning the leader-repeat junction mediate CRISPR adaptation to phage in Streptococcus thermophilus

(2015) Yunzhou Wei et al.   NUCLEIC ACIDS RESEARCH

Different genome stability proteins underpin primed and naïve adaptation inE. coliCRISPR-Cas immunity

(2015) Ivana Ivančić-Baće et al.   NUCLEIC ACIDS RESEARCH

Intrinsic sequence specificity of the Cas1 integrase directs new spacer acquisition

(2015) Clare Rollie et al.   eLife

Conditional tolerance of temperate phages via transcription-dependent CRISPR-Cas targeting

(2014) Gregory W. Goldberg et al.   NATURE

DNA interrogation by the CRISPR RNA-guided endonuclease Cas9

(2014) Samuel H. Sternberg et al.   NATURE

Cas1–Cas2 complex formation mediates spacer acquisition during CRISPR–Cas adaptive immunity

(2014) James K Nuñez et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

An archaeal CRISPR type III-B system exhibiting distinctive RNA targeting features and mediating dual RNA and DNA interference

(2014) Wenfang Peng et al.   NUCLEIC ACIDS RESEARCH

Detection and characterization of spacer integration intermediates in type I-E CRISPR–Cas system

(2014) Zihni Arslan et al.   NUCLEIC ACIDS RESEARCH

Priming in the Type I-F CRISPR-Cas system triggers strand-independent spacer acquisition, bi-directionally from the primed protospacer

(2014) Corinna Richter et al.   NUCLEIC ACIDS RESEARCH

Classification and evolution of type II CRISPR-Cas systems

(2014) Krzysztof Chylinski et al.   NUCLEIC ACIDS RESEARCH

Degenerate target sites mediate rapid primed CRISPR adaptation

(2014) P. C. Fineran et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Adaptation in bacterial CRISPR-Cas immunity can be driven by defective phages

(2014) Alexander P. Hynes et al.   Nature Communications

A novel interference mechanism by a type IIIB CRISPR-Cmr module inSulfolobus

(2013) Ling Deng et al.   MOLECULAR MICROBIOLOGY

RNA-guided editing of bacterial genomes using CRISPR-Cas systems

(2013) Wenyan Jiang et al.   NATURE BIOTECHNOLOGY

Programmable plasmid interference by the CRISPR-Cas system inThermococcus kodakarensis

(2013) Joshua R. Elmore et al.   RNA Biology

Two CRISPR-Cas systems inMethanosarcina mazeistrain Gö1 display common processing features despite belonging to different types I and III

(2013) Lisa Nickel et al.   RNA Biology

Strong bias in the bacterial CRISPR elements that confer immunity to phage

(2013) David Paez-Espino et al.   Nature Communications

CRISPR Interference Can Prevent Natural Transformation and Virulence Acquisition during In Vivo Bacterial Infection

(2012) David Bikard et al.   Cell Host & Microbe

CRISPR Immunity Relies on the Consecutive Binding and Degradation of Negatively Supercoiled Invader DNA by Cascade and Cas3

(2012) Edze R. Westra et al.   MOLECULAR CELL

Characterization of CRISPR RNA processing in Clostridium thermocellum and Methanococcus maripaludis

(2012) Hagen Richter et al.   NUCLEIC ACIDS RESEARCH

Proteins and DNA elements essential for the CRISPR adaptation process in Escherichia coli

(2012) Ido Yosef et al.   NUCLEIC ACIDS RESEARCH

CRISPR Interference Directs Strand Specific Spacer Acquisition

(2012) Daan C. Swarts et al.   PLoS One

Molecular memory of prior infections activates the CRISPR/Cas adaptive bacterial immunity system

(2012) Kirill A. Datsenko et al.   Nature Communications

Persisting Viral Sequences Shape Microbial CRISPR-based Immunity

(2012) Ariel D. Weinberger et al.   PLoS Computational Biology

RNA processing in the minimal organism Nanoarchaeum equitans

(2012) Lennart Randau   GENOME BIOLOGY

Recombination Hotspots and Single-Stranded DNA Binding Proteins Couple DNA Translocation to DNA Unwinding by the AddAB Helicase-Nuclease

(2011) Joseph T.P. Yeeles et al.   MOLECULAR CELL

CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III

(2011) Elitza Deltcheva et al.   NATURE

The Escherichia coli CRISPR System Protects from   Lysogenization, Lysogens, and Prophage Induction

(2010) R. Edgar et al.   JOURNAL OF BACTERIOLOGY

A dual function of the CRISPR-Cas system in bacterial antivirus immunity and DNA repair

(2010) Mohan Babu et al.   MOLECULAR MICROBIOLOGY

Self versus non-self discrimination during CRISPR RNA-directed immunity

(2010) Luciano A. Marraffini et al.   NATURE

Short motif sequences determine the targets of the prokaryotic CRISPR defence system

(2009)   MICROBIOLOGY-SGM

RecBCD Enzyme and the Repair of Double-Stranded DNA Breaks

(2008) M. S. Dillingham et al.   MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS

CRISPR Interference Limits Horizontal Gene Transfer in Staphylococci by Targeting DNA

(2008) Luciano A. Marraffini et al.   SCIENCE