Engineering complex biological systems in bacteria through recombinase-assisted genome engineering

Rapid one-step inactivation of single or multiple genes inEscherichia coli

(2013) Chan Woo Song et al.   Biotechnology Journal

Precise and reliable gene expression via standard transcription and translation initiation elements

(2013) Vivek K Mutalik et al.   NATURE METHODS

Implementation of stable and complex biological systems through recombinase-assisted genome engineering

(2013) Christine Nicole S. Santos et al.   Nature Communications

Genome-scale genetic manipulation methods for exploring bacterial molecular biology

(2012) Alla Gagarinova et al.   Molecular BioSystems

Microbial engineering for the production of advanced biofuels

(2012) Pamela P. Peralta-Yahya et al.   NATURE

Full-length RecE enhances linear-linear homologous recombination and facilitates direct cloning for bioprospecting

(2012) Jun Fu et al.   NATURE BIOTECHNOLOGY

Engineering synthetic recursive pathways to generate non-natural small molecules

(2012) Elizabeth A Felnagle et al.   Nature Chemical Biology

Systems metabolic engineering of microorganisms for natural and non-natural chemicals

(2012) Jeong Wook Lee et al.   Nature Chemical Biology

An Engineered Microbial Platform for Direct Biofuel Production from Brown Macroalgae

(2012) A. J. Wargacki et al.   SCIENCE

Synthetic Biology: Integrated Gene Circuits

(2011) N. Nandagopal et al.   SCIENCE

A Synthetic Genetic Edge Detection Program

(2009) Jeffrey J. Tabor et al.   CELL

Functional metagenomics for enzyme discovery: challenges to efficient screening

(2009) Taku Uchiyama et al.   CURRENT OPINION IN BIOTECHNOLOGY

Programming cells by multiplex genome engineering and accelerated evolution

(2009) Harris H. Wang et al.   NATURE

Stabilized gene duplication enables long-term selection-free heterologous pathway expression

(2009) Keith E J Tyo et al.   NATURE BIOTECHNOLOGY

Automated design of synthetic ribosome binding sites to control protein expression

(2009) Howard M Salis et al.   NATURE BIOTECHNOLOGY

Enzymatic assembly of DNA molecules up to several hundred kilobases

(2009) Daniel G Gibson et al.   NATURE METHODS

Recombineering: a homologous recombination-based method of genetic engineering

(2009) Shyam K Sharan et al.   Nature Protocols

Circular Polymerase Extension Cloning of Complex Gene Libraries and Pathways

(2009) Jiayuan Quan et al.   PLoS One

Cre/lox System and PCR-Based Genome Engineering in Bacillus subtilis

(2008) X. Yan et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Combinatorial engineering of microbes for optimizing cellular phenotype

(2008) Christine Nicole S Santos et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Complete Chemical Synthesis, Assembly, and Cloning of a Mycoplasma genitalium Genome

(2008) D. G. Gibson et al.   SCIENCE