期刊
NATURE METHODS
卷 11, 期 11, 页码 1127-1130出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.3099
关键词
-
资金
- Elite Network of Bavaria (IDK-NBT)
- Society in Science-The Branco Weiss Fellowship
- DFG [Sonderforschungsbereich 1032]
Single-molecule force spectroscopy enables mechanical testing of individual proteins, but low experimental throughput limits the ability to screen constructs in parallel. We describe a microfluidic platform for on-chip expression, covalent surface attachment and measurement of single-molecule protein mechanical properties. A dockerin tag on each protein molecule allowed us to perform thousands of pulling cycles using a single cohesin-modified cantilever. The ability to synthesize and mechanically probe protein libraries enables high-throughput mechanical phenotyping.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据