☆ 4.8 Article

Exploiting single-cell variation for new antibiotics

NATURE CHEMICAL BIOLOGY (2010)

期刊

NATURE CHEMICAL BIOLOGY

卷 6, 期 12, 页码 873-875

出版社

NATURE PUBLISHING GROUP

DOI: 10.1038/nchembio.483

关键词

-

类别

Biochemistry & Molecular Biology

向作者/读者索取更多资源

Protocol

Reagent

摘要

Variations between single members of a bacterial population can lead to antibiotic resistance that is not gene based. The future of effective infectious disease management might depend on a better understanding of this phenomenon and the potential to manipulate both it and microbial population dynamics in general.

作者

我是这篇论文的作者

点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.8

评分不足

次要评分

新颖性

-

重要性

-

科学严谨性

-

评价这篇论文

推荐

Review Immunology

Exploiting Single-Cell Tools in Gene and Cell Therapy

Daniel Bode, Alyssa H. Cull, Juan A. Rubio-Lara, David G. Kent

Summary: The rapid development of single-cell molecular tools has enabled in-depth exploration of individual cell genome, transcriptome, epigenome, and proteome, unraveling the molecular networks of complex biological systems. This technology plays a crucial role in fields with cellular heterogeneity such as stem cell biology, immunology, and tumor cell biology. Moreover, single-cell omics technologies have contributed to the identification of novel disease biomarkers, cellular subsets, therapeutic targets, and diagnostics.

FRONTIERS IN IMMUNOLOGY (2021)

添加到收藏夹

Article Multidisciplinary Sciences

Single-cell genomic variation induced by mutational processes in cancer

Tyler Funnell, Ciara H. O'Flanagan, Marc J. Williams, Andrew McPherson, Steven McKinney, Farhia Kabeer, Hakwoo Lee, Sohrab Salehi, Ignacio Vazquez-Garcia, Hongyu Shi, Emily Leventhal, Tehmina Masud, Peter Eirew, Damian Yap, Allen W. Zhang, Jamie L. P. Lim, Beixi Wang, Jazmine Brimhall, Justina Biele, Jerome Ting, Vinci Au, Michael Van Vliet, Yi Fei Liu, Sean Beatty, Daniel Lai, Jenifer Pham, Diljot Grewal, Douglas Abrams, Eliyahu Havasov, Samantha Leung, Viktoria Bojilova, Richard A. Moore, Nicole Rusk, Florian Uhlitz, Nicholas Ceglia, Adam C. Weiner, Elena Zaikova, J. Maxwell Douglas, Dmitriy Zamarin, Britta Weigelt, Sarah H. Kim, Arnaud Da Cruz Paula, Jorge S. Reis-Filho, Spencer D. Martin, Yangguang Li, Hong Xu, Teresa Ruiz de Algara, So Ra Lee, Viviana Cerda Llanos, David G. Huntsman, Jessica N. McAlpine, Sohrab P. Shah, Samuel Aparicio

Summary: This study reveals the contribution of cell-to-cell structural variation to the phenotypic and evolutionary diversity in human breast cancer and ovarian cancer, and provides insights into the genomic and mutational states of individual cancer cells.

NATURE (2022)

添加到收藏夹

Article Multidisciplinary Sciences

Detection of antimicrobial impact on gram-negative bacterial cell envelope based on single-cell imaging by scanning electron microscopy

Akiko Hisada, Erino Matsumoto, Ryo Hirano, Mami Konomi, Jacques Yaacoub Bou Khalil, Didier Raoult, Yusuke Ominami

Summary: Rapid determination of drug efficacy against bacterial pathogens is crucial for detecting potentially resistant bacteria and optimizing the use of antimicrobials. This study proposes a simplified method for assessing antimicrobial efficacy by detecting immediate damage to bacterial cells using tabletop SEM. The method involves staining unaffected bacterial cell envelopes and whole affected cells, with brighter images indicating lethal damage. By quantifying single-cell images based on brightness, the efficacy of antimicrobials in causing bacterial-cell-envelope damage can be determined.

SCIENTIFIC REPORTS (2023)

添加到收藏夹

Article Chemistry, Multidisciplinary

Enhancement of photocatalytic efficacy by exploiting copper doping in nano-hydroxyapatite for degradation of Congo red dye

Md. Sahadat Hossain, Supanna Malek Tuntun, Newaz Mohammed Bahadur, Samina Ahmed

Summary: This research examines the photocatalytic activity of hydroxyapatite and the improvement of efficiency by doping copper. The study found that copper doping significantly enhanced the photocatalytic efficacy of hydroxyapatite in degrading Congo red dye. Various characterization techniques were used to assess the catalysts, and a relationship was established between the crystallographic parameters and photocatalytic performance.

RSC ADVANCES (2022)

添加到收藏夹

Article Multidisciplinary Sciences

Sequence edition of single domains modulates the final immune and antimicrobial potential of a new generation of multidomain recombinant proteins

Ramon Roca-Pinilla, Ravi Holani, Adria Lopez-Cano, Cristina Saubi, Ricardo Balta-Foix, Eduardo R. Cobo, Elena Garcia-Fruitos, Anna Aris

Summary: Combining multiple innate immune peptides into a single recombinant antimicrobial and immunomodulatory polypeptide has shown potential advantages, with the ability to improve its immunomodulatory and antimicrobial functions by adjusting the sequences of different domains.

SCIENTIFIC REPORTS (2021)

添加到收藏夹

Article Chemistry, Multidisciplinary

Atomic force microscopy and surface plasmon resonance for real-time single-cell monitoring of bacteriophage-mediated lysis of bacteria

Radka Oborilova, Hana Simeckova, Matej Pastucha, Simon Klimovic, Ivana Visova, Jan Pribyl, Hana Vaisocherova-Lisalova, Roman Pantucek, Petr Skladal, Ivana Maslanova, Zdenek Farka

Summary: The growing incidence of multidrug-resistant bacterial strains, such as Staphylococcus aureus, poses a significant challenge in modern medicine. By utilizing atomic force microscopy (AFM) along with surface plasmon resonance (SPR) experiments, the study provides a foundation for understanding the lysis of individual bacteria, particularly S. aureus, which may advance the development of phage therapy.

NANOSCALE (2021)

添加到收藏夹

Article Chemistry, Multidisciplinary

Structural Basis for a Dual Function ATP Grasp Ligase That Installs Single and Bicyclic ω-Ester Macrocycles in a New Multicore RiPP Natural Product

Gengxiang Zhao, Dalibor Kosek, Hong-Bing Liu, Shannon Ohlemacher, Brittney Blackburne, Anastasia Nikolskaya, Kira S. Makarova, Jiadong Sun, Clifton E. Barry, Eugene Koonin, Fred Dyda, Carole A. Bewley

Summary: Chryseoviridin, a newly discovered multicore RiPP, contains four consecutive five- or six-residue macrocycles ending with a microviridin-like core. The crystal structure reveals a significant conformational change in the B-domain of the ATP-grasp ligase CdnC upon substrate binding, providing insights into omega-ester bond formation in RiPP biosynthesis. Experimental validation confirms a model where macrocyclization occurs in the N- to C-terminal direction for core peptides containing multiple Thr/Ser-to-Asp macrocycles.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2021)

添加到收藏夹

Review Medicine, Research & Experimental

Single-cell sequencing: New insights for intervertebral disc degeneration

Xinli Hu, Zheng Wang, Haojie Zhang, Peng Cui, Yongjin Li, Xiaolong Chen, Chao Kong, Wei Wang, Shibao Lu

Summary: Over the past decade, single-cell RNA sequencing (scRNA-seq) has revolutionized the study of disease mechanisms. It has been used to identify unique cell types, characterize cellular heterogeneity, and detect transcriptional alterations or perturbed pathways under pathological conditions. In the context of intervertebral disc degeneration (IVDD), scRNA-seq has provided insights into the populations of specific cell types and their interactions, leading to progress in IVDD treatment and the discovery of novel therapeutic targets.

BIOMEDICINE & PHARMACOTHERAPY (2023)

添加到收藏夹

Article Multidisciplinary Sciences

Dissecting human population variation in single-cell responses to SARS-CoV-2

Yann Aquino, Aurelie Bisiaux, Zhi Li, Mary O'Neill, Javier Mendoza-Revilla, Sarah Helene Merkling, Gaspard Kerner, Milena Hasan, Valentina Libri, Vincent Bondet, Nikaia Smith, Camille de Cevins, Mickael Menager, Francesca Luca, Roger Pique-Regi, Giovanna Barba-Spaeth, Stefano Pietropaoli, Olivier Schwartz, Geert Leroux-Roels, Cheuk-Kwong Lee, Kathy Leung, Joseph T. Wu, Malik Peiris, Roberto Bruzzone, Laurent Abel, Jean-Laurent Casanova, Sophie A. Valkenburg, Darragh Duffy, Etienne Patin, Maxime Rotival, Lluis Quintana-Murci

Summary: Humans exhibit significant individual clinical variability after SARS-CoV-2 infection, and the genetic and immunological basis for this variability is being studied. In this study, researchers analyzed single-cell RNA sequencing data from 222 healthy donors with diverse ancestries, who were exposed to SARS-CoV-2 or influenza A virus. They found that SARS-CoV-2 induces weaker but more heterogeneous immune responses compared to influenza A virus, and identified population differences in transcriptional responses to viruses driven by changes in cell composition. The study also highlights the impact of genetic variants on immune responses and the role of natural selection in population differences. Furthermore, they uncover the cellular and molecular mechanisms by which Neanderthal introgression has influenced immune functions. Additionally, the researchers reveal an overlap between the genetic basis of immune responses to SARS-CoV-2 and COVID-19 severity, shedding light on the factors contributing to disparities in COVID-19 risk.

NATURE (2023)

添加到收藏夹

Review Biochemistry & Molecular Biology

Alternative Splicing Variation: Accessing and Exploiting in Crop Improvement Programs

Sangam L. Dwivedi, Luis Felipe Quiroz, Anireddy S. N. Reddy, Charles Spillane, Rodomiro Ortiz

Summary: Alternative splicing (AS) is a gene regulatory mechanism that modulates gene expression in plants. AS generates multiple mRNAs to regulate transcriptome and proteome diversity. Advances in sequencing and computational methods enable the quantification and visualization of AS-based variation in plant growth, development, and stress tolerance. Understanding and utilizing this variation can enhance climate resilience, productivity, and nutritional quality of staple food crops. AS also contributes to heterosis, plant symbiosis, and the connection between core clock genes and environmental cues.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2023)

添加到收藏夹

Article Microbiology

Understanding Beta-Lactam-Induced Lysis at the Single-Cell Level

Felix Wong, Sean Wilson, Ralf Helbig, Smitha Hegde, Olha Aftenieva, Hai Zheng, Chenli Liu, Teuta Pilizota, Ethan C. Garner, Ariel Amir, Lars D. Renner

Summary: By experimentally analyzing the dynamics of lysis in hundreds of single Escherichia coli cells, it was found that turgor pressure is the only factor that robustly modulates lysis, while mechanosensitive channels do not modulate lysis due to insufficiently fast solute outflow, and cell shape changes result in more severe cellular lesions but do not influence the dynamics of lysis. These results provide insights into a single-cell view of bacterial cell lysis and highlight approaches of combatting antibiotic tolerance to beta-lactams by targeting cellular turgor.

FRONTIERS IN MICROBIOLOGY (2021)

添加到收藏夹

Article Chemistry, Multidisciplinary

Inherently Emissive Puromycin Analogues for Live Cell Labelling

Kaivin Hadidi, Kfir B. Steinbuch, Lara E. Dozier, Gentry N. Patrick, Yitzhak Tor

Summary: Puromycin derivatives modified with azetidine and 3,3-difluoroazetidine exhibit translation inhibition and bactericidal activity similar to natural antibiotic. These analogues can puromycylate nascent peptides, generating emissive products without follow-up chemistry. The 3,3-difluoroazetidine-containing analogue can fluorescently label newly translated peptides and be visualized in live and fixed cells.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2023)

添加到收藏夹

Article Chemistry, Analytical

Characterization of Single-Cell Osmotic Swelling Dynamics for New Physical Biomarkers

Apresio K. Fajrial, Kun Liu, Yu Gao, Junhao Gu, Richard Lakerveld, Xiaoyun Ding

Summary: Characterization of physical biomarkers of cells through osmotic swelling can evaluate unique swelling dynamics of different cell types with high accuracy. This method can also detect changes in cellular mechanical properties due to cytoskeleton disruption, offering potential applications in both biological studies and clinical practice. Its simplicity makes swelling dynamics an invaluable label-free physical biomarker for cells.

ANALYTICAL CHEMISTRY (2021)

添加到收藏夹

Article Microbiology

Visualizing Pyrazinamide Action by Live Single-Cell Imaging of Phagosome Acidification and Mycobacterium tuberculosis pH Homeostasis

Pierre Santucci, Beren Aylan, Laure Botella, Elliott M. Bernard, Claudio Bussi, Enrica Pellegrino, Natalia Athanasiadi, Maximiliano G. Gutierrez

Summary: This study investigates the effect of intracellular environments on the efficacy of antibiotics against Mycobacterium tuberculosis. By using a dual live imaging approach, the researchers found that intracellular acidification is a prerequisite for the antibacterial efficacy of pyrazinamide, a first-line anti-TB drug.

MBIO (2022)

添加到收藏夹

Article Engineering, Environmental

New use for biochar derived from bovine manure for tetracycline removal

Jiawei Zhao, Feng Gao, Yue Sun, Wanyi Fang, Xiaohan Li, Yingjie Dai

Summary: The study investigated the physicochemical properties of bovine manure biochar obtained at different temperatures and their adsorption of tetracycline. The results showed that both BC-500 and BC-700 had high TC removal rates, with different maximum adsorption capacities, and were well described by pseudo-second order kinetics. Even after multiple cycles, the biochar still maintained satisfactory removal efficiencies, mainly through hydrophobic interactions and pi-pi electron donor acceptor interactions.

JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING (2021)

添加到收藏夹

Article Microbiology

Mutations in the pantothenate kinase of Plasmodium falciparum confer diverse sensitivity profiles to antiplasmodial pantothenate analogues

Erick T. Tjhin, Christina Spry, Alan L. Sewell, Annabelle Hoegl, Leanne Barnard, Anna E. Sexton, Ghizal Siddiqui, Vanessa M. Howieson, Alexander G. Maier, Darren J. Creek, Erick Strauss, Rodolfo Marquez, Karine Auclair, Kevin J. Saliba

PLOS PATHOGENS (2018)

添加到收藏夹

Article Chemistry, Medicinal

Developing Pantetheinase-Resistant Pantothenamide Antibacterials: Structural Modification Impacts on PanK Interaction and Mode of Action

Leanne Barnard, Konrad J. Mostert, Willem A. L. van Otterlo, Erick Strauss

ACS INFECTIOUS DISEASES (2018)

添加到收藏夹

Article Chemistry, Multidisciplinary

Modular Enzymatic Cascade Synthesis of Vitamin B5 and Its Derivatives

Mohammad Z. Abidin, Thangavelu Saravanan, Jielin Zhang, Pieter G. Tepper, Erick Strauss, Gerrit J. Poelarends

CHEMISTRY-A EUROPEAN JOURNAL (2018)

添加到收藏夹

Article Biochemistry & Molecular Biology

Probing the ligand preferences of the three types of bacterial pantothenate kinase

Jinming Guan, Leanne Barnard, Jeanne Cresson, Annabelle Hoegl, Justin H. Chang, Erick Strauss, Karine Auclair

BIOORGANIC & MEDICINAL CHEMISTRY (2018)

添加到收藏夹

Article Chemistry, Medicinal

Toward a Stable and Potent Coenzyme A-Targeting Antiplasmodial Agent: Structure-Activity Relationship Studies of N-Phenethyl-α-methyl-pantothenamide

Christina Spry, Leanne Barnard, Michelle Kok, Andrew K. Powell, Darvina Mahesh, Erick T. Tjhin, Kevin J. Saliba, Erick Strauss, Marianne de Villiers

ACS INFECTIOUS DISEASES (2020)

添加到收藏夹

Article Multidisciplinary Sciences

Role of allosteric switches and adaptor domains in long-distance cross-talk and transient tunnel formation

Nandini Sharma, Navjeet Ahalawat, Padmani Sandhu, Erick Strauss, Jagannath Mondal, Ruchi Anand

SCIENCE ADVANCES (2020)

添加到收藏夹

Review Immunology

Vitamin in the Crosshairs: Targeting Pantothenate and Coenzyme A Biosynthesis for New Antituberculosis Agents

Hailey S. Butman, Timothy J. Kotze, Cynthia S. Dowd, Erick Strauss

FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY (2020)

添加到收藏夹

Article Biochemistry & Molecular Biology

The Coenzyme A Level Modulator Hopantenate (HoPan) Inhibits Phosphopantotenoylcysteine Synthetase Activity

Konrad J. Mostert, Nandini Sharma, Marianne van der Zwaag, Roxine Staats, Lizbe Koekemoer, Ruchi Anand, Ody C. M. Sibon, Erick Strauss

Summary: This study reveals that the mechanism of action of the pantothenate analogue hopantenate (HoPan) is more complex than previously thought, showing that it relies on pantothenate kinase for metabolic activation rather than inhibiting it. The findings also provide insights into evaluating factors contributing to the potency of CoA-directed inhibitors, which will be valuable for the rational development of potential therapies for human genetic and infectious diseases in the future.

ACS CHEMICAL BIOLOGY (2021)

添加到收藏夹

Article Microbiology

Evaluating the Genetic Capacity of Mycoplasmas for Coenzyme A Biosynthesis in a Search for New Anti-mycoplasma Targets

Tertius Alwyn Ras, Erick Strauss, Annelise Botes

Summary: Mycoplasmas have the genetic capacity to synthesize CoA, with variations in the prevalence of relevant genes across species. Despite no correlation between the existence of certain genes and phylogenetic position, the enzyme DPCK was found to be the most common in the biosynthesis pathway, suggesting it may be a key target for new anti-mycoplasma agents.

FRONTIERS IN MICROBIOLOGY (2021)

添加到收藏夹

Article Biochemistry & Molecular Biology

Coenzyme A precursors flow from mother to zygote and from microbiome to host

Yi Yu, Marianne van der Zwaag, Jouke Jan Wedman, Hjalmar Permentier, Niels Plomp, Xiu Jia, Bart Kanon, Ellie Eggens-Meijer, Girbe Buist, Hermie Harmsen, Jan Kok, Joana Falcao Salles, Bregje Wertheim, Susan J. Hayflick, Erick Strauss, Nicola A. Grzeschik, Hein Schepers, Ody C. M. Sibon

Summary: Coenzyme A (CoA) is essential for metabolism and protein acetylation. Current knowledge suggests that each cell obtains CoA through biosynthesis, but recent studies have shown the presence of additional CoA-generating mechanisms, indicating a more complex system for CoA homeostasis. In this study, researchers discovered pathways for CoA generation through inter-organismal flows of CoA precursors. Fruit flies with a genetic block in CoA biosynthesis were used to demonstrate that progeny can obtain CoA precursors from maternal sources for survival during embryonal and early larval development. Later in life, the microbiome can provide necessary CoA building blocks to the host, enabling normal development to continue. This study reveals the presence of stable, long-lasting CoA precursor flows between living organisms, indicating complex strategies for maintaining CoA homeostasis.

MOLECULAR CELL (2022)

添加到收藏夹

Article Chemistry, Medicinal

Multifaceted Target Specificity Analysis as a Tool in Antimicrobial Drug Development: Type III Pantothenate Kinases as a Case Study

Siobhan Ernan Brigg, Lizbe Koekemoer, Leisl A. A. Brand, Erick Strauss

Summary: The research proposes the use of Multifaceted Target Specificity Analysis (MTSA) to assess the activity of a new antimicrobial drug against closely related organisms. By studying the case of type III pantothenate kinase (PanK(III)) as a target, it aims to determine if targeting a specific organism's PanK(III) would result in a narrow- or broad-spectrum agent. MTSA is suggested as a valuable tool for guiding target-based antimicrobial drug development initiatives.

CHEMMEDCHEM (2023)

添加到收藏夹

Article Chemistry, Organic

The broad amine scope of pantothenate synthetase enables the synthesis of pharmaceutically relevant amides

Mohammad Z. Abidin, Thangavelu Saravanan, Erick Strauss, Gerrit J. Poelarends

Summary: Pantothenate synthetase from Escherichia coli can efficiently synthesize pharmaceutically-relevant vitamin B-5 antimetabolites by accepting a wide range of structurally diverse amines in addition to its natural amine substrate, beta-alanine. This biocatalytic amide synthesis strategy shows promise in the search for new antimicrobials targeting coenzyme A biosynthesis and utilization.

ORGANIC & BIOMOLECULAR CHEMISTRY (2021)

添加到收藏夹

Article Biochemistry & Molecular Biology

Voices of chemical biology

Benjamin Davis, Keri Backus, Georg Winter, Roberto Chica, Dan Li, Sang Yup Lee, Chuan He, Amy Weeks, Christopher Overall, Shinya Hagihara, Ben Thuronyi, Siddhesh Kamat, Ling-Ling Chen, Ramon Hurtado Guerrero, Shao Yao, Lara K. Mahal, Christopher Voigt, Christina Woo, Erick Strauss, Kazuya Kikuchi, Timothy Dore, Sheena Radford, Xiang David Li, Won Do Heo, Giulio Superti-Furga, Tara Deans, Vsevolod Belousov, Megan Matthews, Colin Jackson, Shiva Malek, Herbert Waldmann, Anna Rising, Michael Jewett, Dimitrios Stamou, Emily Parker, Mario Murakami, Karen Polizzi, Itaru Hamachi, Tobias Erb, Chirlmin Joo, Motonari Uesugi, Rab Prinjha, Gidi Rechavi, Roberto Solano, Brenda Schulman, Yael David, Rob Oslund

Summary: In the last five years, the most exciting research achievements or technological innovations in chemical biology have garnered attention from many chemical biologists.

NATURE CHEMICAL BIOLOGY (2021)

添加到收藏夹

Article Biochemistry & Molecular Biology

Overcoming synthetic challenges in targeting coenzyme A biosynthesis with the antimicrobial natural product CJ-15,801

Riyad Domingo, Renier van der Westhuyzen, Anton R. Hamann, Konrad J. Mostert, Leanne Barnard, Tanya Paquet, Erick T. Tjhin, Kevin J. Saliba, Willem A. L. van Otterlo, Erick Strauss

MEDCHEMCOMM (2019)

添加到收藏夹

Article Biology

Vnn1 pantetheinase limits the Warburg effect and sarcoma growth by rescuing mitochondrial activity

Caroline Giessner, Virginie Millet, Konrad J. Mostert, Thomas Gensollen, Thien-Phong Vu Manh, Marc Garibal, Binta Dieme, Noudjoud Attaf-Bouabdallah, Lionel Chasson, Nicolas Brouilly, Caroline Laprie, Tom Lesluyes, Jean Yves Blay, Laetitia Shintu, Jean Charles Martin, Erick Strauss, Franck Galland, Philippe Naquet

LIFE SCIENCE ALLIANCE (2018)

添加到收藏夹

暂无数据

© Peeref 2019-2024. All rights reserved.