Article
Multidisciplinary Sciences
Claire Fielding, Andres Garcia-Garcia, Claudia Korn, Stephen Gadomski, Zijian Fang, Juan L. Reguera, Jose A. Perez-Simon, Berthold Gottgens, Simon Mendez-Ferrer
Summary: Cholinergic signals in the bone marrow preserve haematopoietic stem cell quiescence and self-renewal under proliferative stress.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Shuxian Dong, Qian Wang, Yun-Ruei Kao, Antonio Diaz, Inmaculada Tasset, Susmita Kaushik, Victor Thiruthuvanathan, Aliona Zintiridou, Edward Nieves, Monika Dzieciatkowska, Julie A. Reisz, Evripidis Gavathiotis, Angelo D'Alessandro, Britta Will, Maria Cuervo
Summary: Activation of hematopoietic stem cells involves molecular adaptations, with Chaperone-Mediated Autophagy (CMA) playing a role in sustaining HSC function and declining with age. Restoring old HSC functionality through CMA activation suggests it may be a promising therapeutic target for conditions like aging or stem-cell transplantation.
Article
Cell Biology
Idil Orhon, Cecilia Rocchi, Beatriz Villarejo-Zori, Paola Serrano Martinez, Mirjam Baanstra, Uilke Brouwer, Patricia Boya, Rob Coppes, Fulvio Reggiori
Summary: In dormant salivary gland stem cells, autophagy has a slower flux compared to self-renewing cells; however, enhancement of autophagy upon activation promotes self-renewal and tissue regeneration. Autophagy is a key pathway in activating self-renewal in low proliferative adult tissues, with potential for promoting tissue regeneration through pharmacological manipulation.
Review
Cell Biology
Yavor K. Bozhilov, Ian Hsu, Elizabeth J. Brown, Adam C. Wilkinson
Summary: The haematopoietic system is crucial for our health and survival, consisting of various mature blood and immune cell types that support homeostasis. In vitro culture systems have been developed to expand and differentiate haematopoietic stem and progenitor cells, providing models for studying human haematopoiesis. These systems also have potential applications in transplantation and transfusion medicine.
Article
Cell Biology
Dehao Huang, Qianhao Zhao, Mengyun Zhang, Qitong Weng, Qi Zhang, Kaitao Wang, Fang Dong, Hui Cheng, Fangxiao Hu, Jinyong Wang
Summary: In this study, the researchers used a mouse model with conditional expression of Hoxb5 to investigate its effect on multipotent blood progenitor cells. They found that induced expression of Hoxb5 in these cells led to the generation of a new cell type that can repopulate long-term multilineage haematopoiesis. RNA-seq analysis showed that these cells exhibited features of DNA replication and cell division.
CELL PROLIFERATION
(2022)
Article
Multidisciplinary Sciences
Jette Lengefeld, Chia-Wei Cheng, Pema Maretich, Marguerite Blair, Hannah Hagen, Melanie R. McReynolds, Emily Sullivan, Kyra Majors, Christina Roberts, Joon Ho Kang, Joachim D. Steiner, Teemu P. Miettinen, Scott R. Manalis, Adam Antebi, Sean J. Morrison, Jacqueline A. Lees, Laurie A. Boyer, Omer H. Yilmaz, Angelika Amon
Summary: The size of stem cells plays a crucial role in their function, with small size being important for maintaining stem cell potential. Enlargement of hematopoietic stem cells under conditions of decreased function is associated with reduced proliferation and altered metabolism. Preventing stem cell enlargement can help prevent the loss of stem cell potential in exhausting conditions.
Article
Cell Biology
Joey J. Ghersi, Gabriel Baldissera, Jared Hintzen, Stephanie A. Luff, Siyuan Cheng, Ivan Fan Xia, Christopher M. Sturgeon, Stefania Nicoli
Summary: Ghersi et al. have found that the diversity of haematopoietic stem and progenitor cells is established at the haemogenic endothelium level and is regulated by microRNA-128-mediated modulation of Wnt and Notch signalling. Haematopoietic stem and progenitor cells (HSPCs) generate different lineages and are produced in the embryo through the transformation of haemogenic endothelial cells in the aorta-gonad-mesonephros (AGM). The researchers discovered that loss of microRNA-128 leads to an expansion of HSPCs in the AGM with different cell cycle states and a bias towards erythroid and lymphoid progenitors.
NATURE CELL BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Xin Gao, Dachuan Zhang, Chunliang Xu, Huihui Li, Kathleen M. Caron, Paul S. Frenette
Summary: Nociceptive nerves play a role in enforcing HSC mobilization through CGRP secretion, collaborating with sympathetic nerves to maintain HSCs in the bone marrow. CGRP directly activates HSCs via the G alpha(s)/adenylyl cyclase/cAMP pathway, and consumption of capsaicin-containing food enhances HSC mobilization in mice.
Article
Multidisciplinary Sciences
Joachim Hanna, Flavio Beke, Louise M. O'Brien, Chrysa Kapeni, Hung-Chang Chen, Valentina Carbonaro, Alexander B. Kim, Kamal Kishore, Timon E. Adolph, Mikkel-Ole Skjoedt, Karsten Skjoedt, Marc de la Roche, Maike de la Roche
Summary: The study implicates Hedgehog signaling in Th17 polarization and intestinal immunopathology, suggesting the potential therapeutic use of Hedgehog inhibitors in the treatment of inflammatory bowel disease.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Nathaniel Vincent Mon Pere, Tom Lenaerts, Jorge Manuel dos Santos Pacheco, David Dingli
Summary: Human hematopoiesis shows resilience to disruptions, but certain disorders can push the system beyond its natural adaptability, resulting in abnormal blood circulation. Studies have found that a stable response to perturbations requires simultaneous adaptation of cell differentiation and self-renewal rates, and under conditions of continuous disruption, compartment cell numbers evolve to new stable states.
Article
Multidisciplinary Sciences
Qing Guo, Shuai Shen, Gefei Guan, Chen Zhu, Cunyi Zou, Jingyuan Cao, Wen Cheng, Xiaoyan Xu, Juanhan Yu, Zhiguo Lin, Guoli Wang, Ling Chen, Peng Cheng, Anhua Wu
Summary: Glioblastoma (GBM) shares common signaling pathways involving TIM-3, an immune checkpoint, between tumor and non-tumor cells. The study reveals that TIM-3 in glioma cells not only regulates the malignant behaviors of glioma cells but also induces macrophage migration and transition to an anti-inflammatory/protumorigenic phenotype through the TIM-3/IL6 signal. Blocking this feedback loop may provide a novel therapeutic strategy for GBM.
Article
Cell Biology
Manuel Tavares, Garima Khandelwal, Joanne Muter, Keijo Viiri, Manuel Beltran, Jan J. Brosens, Richard G. Jenner
Summary: The fusion protein JAZF1-SUZ12 dysregulates PRC2 composition, gene expression, and cell differentiation, and may play an important role in oncogenesis.
Article
Multidisciplinary Sciences
Abha S. Bais, Debora M. Cerqueira, Andrew Clugston, Andrew J. Bodnar, Jacqueline Ho, Dennis Kostka
Summary: The kidney is a complex organ with distinct cell types during development, and cell cycle activity is closely related to the differentiation of nephron progenitors and kidney morphogenesis.
SCIENTIFIC REPORTS
(2021)
Article
Cell Biology
Fan Yang, Eoin C. Whelan, Xuebing Guan, Bingquan Deng, Shu Wang, Jiachen Sun, Mary R. Avarbock, Xin Wu, Ralph L. Brinster
Summary: Overexpression of Fgf9 in vivo results in arrested spermatogenesis and accumulation of undifferentiated spermatogonia; exposure of germ cell cultures to FGF9 leads to increased numbers of SSCs over time; inhibition of p38 MAPK phosphorylation negates the growth advantage provided by FGF9.
CELL PROLIFERATION
(2021)
Article
Medicine, Research & Experimental
Yihong Guan, Metis Hasipek, Dongxu Jiang, Anand D. Tiwari, Dale R. Grabowski, Simona Pagliuca, Sunisa Kongkiatkamon, Bhumika Patel, Salendra Singh, Yvonne Parker, Thomas LaFramboise, Daniel Lindner, Mikkael A. Sekeres, Omar Y. Mian, Yogen Saunthararajah, Jaroslaw P. Maciejewski, Babal K. Jha
Summary: Eltrombopag binds to the TET2 catalytic domain and inhibits its dioxygenase activity, leading to the expansion of normal hematopoietic stem cells and the prevention of neoplastic evolution in TET2 mutant malignant myeloid cells. This finding provides a scientific rationale for the treatment of myeloid malignancies associated with TET2 mutations or TET deficiency.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
Article
Biochemical Research Methods
Tonmoy Chakraborty, Meghan K. Driscoll, Elise Jeffery, Malea M. Murphy, Philippe Roudot, Bo-Jui Chang, Saumya Vora, Wen Mai Wong, Cara D. Nielson, Hua Zhang, Vladimir Zhemkov, Chitkale Hiremath, Estanislao Daniel De la Cruz, Yating Yi, Ilya Bezprozvanny, Hu Zhao, Raju Tomer, Rainer Heintzmann, Julian P. Meeks, Denise K. Marciano, Sean J. Morrison, Gaudenz Danuser, Kevin M. Dean, Reto Fiolka
Article
Multidisciplinary Sciences
Yang Liu, Phong T. Nguyen, Xun Wang, Yuting Zhao, Corbin E. Meacham, Zhongju Zou, Bogdan Bordieanu, Manuel Johanns, Didier Vertommen, Tobias Wijshake, Herman May, Guanghua Xiao, Sanae Shoji-Kawata, Mark H. Rider, Sean J. Morrison, Prashant Mishra, Beth Levine
Article
Multidisciplinary Sciences
Alpaslan Tasdogan, Brandon Faubert, Vijayashree Ramesh, Jessalyn M. Ubellacker, Bo Shen, Ashley Solmonson, Malea M. Murphy, Zhimin Gu, Wen Gu, Misty Martin, Stacy Y. Kasitinon, Travis Vandergriff, Thomas P. Mathews, Zhiyu Zhao, Dirk Schadendorf, Ralph J. DeBerardinis, Sean J. Morrison
Article
Biology
Andrew W. DeVilbiss, Zhiyu Zhao, Misty S. Martin-Sandoval, Jessalyn M. Ubellacker, Alpaslan Tasdogan, Michalis Agathocleous, Thomas P. Mathews, Sean J. Morrison
Summary: Little is known about the metabolic regulation of rare cell populations, but a new method involving hydrophilic liquid interaction chromatography and high-sensitivity orbitrap mass spectrometry detected 160 metabolites in 10,000 hematopoietic stem cells, revealing more glycolytic and lipid intermediates. Improved chromatographic separation, increased mass resolution, minimized ion suppression, and eliminated sample drying allowed for routine metabolomic analysis of rare cells from tissues.
Editorial Material
Parasitology
George R. Wendt, Michael L. Reese, James J. Collins
Summary: SchistoCyte Atlas is a web-based platform for exploring gene expression in adult Schistosoma mansoni, which can help non-specialists to better understand the biology of these devastating parasites.
TRENDS IN PARASITOLOGY
(2021)
Letter
Cell & Tissue Engineering
Eric Anthony, Robin Lovell-Badge, Sean J. Morrison
Review
Biochemical Research Methods
Brandon Faubert, Alpaslan Tasdogan, Sean J. Morrison, Thomas P. Mathews, Ralph J. DeBerardinis
Summary: This protocol assesses metabolism in intact tumors by using stable isotope-labeled nutrients delivered through bolus injection and continuous infusion in mice and humans. The study focuses on metabolic adaptations of cancer cells to meet energetic demands imposed by dysregulated growth. The methods involve introducing labeled nutrients into the circulation prior to surgical resection and analyzing isotope labeling patterns in metabolic intermediates.
Article
Multidisciplinary Sciences
Jingzhu Zhang, Adi Cohen, Bo Shen, Liming Du, Alpaslan Tasdogan, Zhiyu Zhao, Elizabeth J. Shane, Sean J. Morrison
Summary: The study revealed that PTH promotes osteolectin expression and its interaction mediates part of the bone formation response to PTH, while SOSTi does not affect osteolectin levels or require it for bone formation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Xiangguo Shi, Yajian Jiang, Ayumi Kitano, Tianyuan Hu, Rebecca L. Murdaugh, Yuan Li, Kevin A. Hoegenauer, Rui Chen, Koichi Takahashi, Daisuke Nakada
Summary: Metabolic dysregulation is the basis for malignant phenotypes of cancer stem cells, and NAD(+) metabolism enables AML to evade apoptosis. NMNAT1 is identified as a therapeutic target for AML progression and chemoresistance by maintaining NAD(+).
Article
Biology
Yuewei Wang, Tong Huo, Yu-Jung Tseng, Lan Dang, Zhili Yu, Wenjuan Yu, Zachary Foulks, Rebecca L. Murdaugh, Steven J. Ludtke, Daisuke Nakada, Zhao Wang
Summary: In this study, the researchers characterized platelets in a mouse model of AML using Cryo-ET and found that platelets in pre-leukemic cells exhibited abnormal mitochondria structures, suggesting that platelet alterations may be used as a biomarker for early diagnosis of AML.
COMMUNICATIONS BIOLOGY
(2022)
Article
Cell Biology
Corbin E. Meacham, Elise C. Jeffery, Rebecca J. Burgess, Charukesi D. Sivakumar, Madison A. Arora, Anne Marie Stanley, Emily M. Colby, Genevieve M. Crane, Zhiyu Zhao, Sean J. Morrison
Summary: The adiponectin receptors play a crucial role in protecting hematopoietic stem cells from inflammation by suppressing chronic inflammatory signaling from immune effector cells. They promote stem cell quiescence and self-renewal by suppressing the expression of inflammatory cytokines in myeloid cells and T cells. Without these receptors, hematopoietic stem cells are chronically activated, leading to reduced self-renewal potential and depletion during aging.
NATURE CELL BIOLOGY
(2022)
Review
Cell Biology
Corbin E. Meacham, Andrew W. DeVilbiss, Sean J. Morrison
Summary: The metabolism of somatic stem cells plays a crucial role in their long-term maintenance, activation, proliferation, and differentiation. Understanding the metabolic regulation in stem cells can lead to new opportunities in manipulating their functions, which can have applications in tissue regeneration and cancer prevention. Recent evidence suggests that stem cells have distinct metabolic characteristics compared to restricted progenitors and that metabolic changes influence tissue homeostasis and regeneration. Stem cell metabolism is influenced by the microenvironment and can interact with other cells in their niches.
NATURE REVIEWS MOLECULAR CELL BIOLOGY
(2022)
Article
Cell & Tissue Engineering
Elise C. Jeffery, Terry L. A. Mann, Jade A. Pool, Zhiyu Zhao, Sean J. Morrison
Summary: The contribution of skeletal stem/progenitor cells (SSCs) from bone marrow and periosteum to bone repair has been investigated. During steady-state conditions, new bone primarily arises from bone marrow SSCs. However, after bone injuries, different populations of SSCs play distinct roles in bone repair, with periosteal cells primarily involved in non-stabilized fracture repair.
Article
Multidisciplinary Sciences
Jingzhu Zhang, Liming Du, Bethany Davis, Zhimin Gu, Junhua Lyu, Zhiyu Zhao, Jian Xu, Sean J. Morrison, Francesca V. Mariani
Summary: Osteolectin is a growth factor that activates the Wnt pathway and promotes bone elongation by binding to Integrin a11. Its deficiency leads to shorter bones in mice, and reduced growth plate chondrocyte proliferation and bone elongation. Furthermore, a single-nucleotide variant associated with reduced height and plasma Osteolectin levels was found downstream of Osteolectin. This study establishes Osteolectin/Integrin a11 as regulators of bone elongation and body length in both mice and humans.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Multidisciplinary Sciences
George Wendt, Lu Zhao, Rui Chen, Chenxi Liu, Anthony J. O'Donoghue, Conor R. Caffrey, Michael L. Reese, James J. Collins