Article
Chemistry, Analytical
Nadia Farsaeivahid, Christian Grenier, Sheyda Nazarian, Ming L. L. Wang
Summary: A label-free rapid electrochemical biosensor for detecting SARS-CoV-2 in human saliva is reported. The sensor utilizes a new chemical construct of gold nanoparticles and thionine immobilized on functionalized carbon nanotubes for high-performance biosensing. The sensor shows promising results with a short response time and a low limit of detection, making it a potential device for the label-free electrochemical detection of SARS-CoV-2 and other viruses.
Article
Engineering, Biomedical
Congran Jin, Ziqian Wu, John H. Molinski, Junhu Zhou, Yundong Ren, John X. J. Zhang
Summary: Advancement in material science, particularly in the development of nanomaterials, has led to the creation of plasmonic biosensors that enable real-time diagnosis of various diseases through biomarker screening. These versatile and portable devices can be used in point-of-care settings, allowing for convenient and efficient testing of biomarkers in homes or elsewhere. This article reviews the recent progress in the design of these biosensors, including the development of nanomaterials and the applications in point-of-care diagnosis. Additionally, challenges and opportunities, such as incorporating machine learning algorithms and the testing of Covid-19, are discussed.
MATERIALS TODAY BIO
(2022)
Article
Biochemical Research Methods
Hanyuan Zhang, Alanna M. Klose, Benjamin L. Miller
Summary: The study utilized glycan-based receptor analogue microarrays on the AIR platform to achieve label-free, multiplex detection and discrimination of human and avian influenza viruses. The method can be expanded to systematically evaluate influenza virus adaptation in humans, facilitating global influenza surveillance and serving as a new tool enabling quick reactions in the face of a pandemic outbreak for health organizations, governments, research institutes, and laboratories.
BIOCONJUGATE CHEMISTRY
(2021)
Article
Chemistry, Analytical
Rong Zhu, Han Jiang, Chaoyou Li, Yan Li, Min Peng, Junfeng Wang, Qian Wu, Chao Yan, Qingli Bo, Jie Wang, Chenlin Shen, Panzhu Qin
Summary: This study presents a point-of-care lateral flow biosensor based on CRISPR/Cas9 recognition for the detection of Mycoplasma pneumoniae. The biosensor utilizes specific primer set and CRISPR/Cas9 for secondary recognition, resulting in high amplification efficiency and a low detection limit of 3 copies at a preamplification temperature of 25 degrees C. The practical application of the biosensor demonstrates 100% accuracy in testing 43 M. pneumoniae-infected specimens and 80 uninfected specimens. The entire detection process can be completed in 30 minutes.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Cynthia Martinez-Liu, Carlos Machain-Williams, Natalia Martinez-Acuna, Sonia Lozano-Sepulveda, Kame Galan-Huerta, Daniel Arellanos-Soto, Mayra Melendez-Villanueva, Diana Avalos-Nolazco, Katya Perez-Ibarra, Sergio Galindo-Rodriguez, Aurora de Jesus Garza-Juarez, Ana Maria Rivas-Estilla
Summary: In this study, a biosensor based on gold nanoparticles and antibody was designed for the detection of dengue virus in vitro, showing significant differences in size and characteristics between different conjugates and naked AuNPs. The biosensor was further used in the development of a lateral flow assay for detecting flavivirus envelope protein in isolated DENV samples, with promising results for future dengue detection.
Article
Chemistry, Analytical
Emily Kerr, Ryan Farr, Egan H. Doeven, Yi Heng Nai, Richard Alexander, Rosanne M. Guijt, Beatriz Prieto-Simon, Paul S. Francis, Megan Dearnley, David J. Hayne, Luke C. Henderson, Nicolas H. Voelcker
Summary: The sensor demonstrated in this study combines an ECL molecular beacon with magnetic bead based extraction of the miRNA target sequence to provide a sensitive and portable solution for miRNA-21 detection. The sensor does not require intercalating labels, reporter probes, added enzymes or hairpin primers for target amplification, offering a simple and robust strategy for miRNA detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Wenjuan Xu, Jiayao Liu, Dan Song, Chunsheng Li, Anna Zhu, Feng Long
Summary: The newly developed all-fiber Fresnel reflection microfluidic biosensor integrates an all-fiber optical system, microfluidic chip, and multimode fiber bio-probe, enabling rapid, sensitive, and label-free detection of SARS-CoV-2 antibodies. This system simplifies the structure and improves light transmission efficiency, making it suitable for on-site testing of nanoliter samples.
Article
Nanoscience & Nanotechnology
Shahrzad Molavi, Ali Bozorg, Yaser Abdi
Summary: This article introduces a label-free and antibody-free gated device capable of sensitive and selective detection of bacteria. By utilizing the unique electrical characteristics induced by bacteria on the device, selective detection is achieved, providing a platform for single-cell detection.
ACS APPLIED NANO MATERIALS
(2021)
Article
Chemistry, Physical
Moonbong Jang, Myoungro Lee, Hiesang Sohn, Chulhwan Park, Taek Lee
Summary: We developed a rapid electrical biosensor for detecting Zika virus infection using a truncated DNA aptamer and alternating current electrothermal flow technique. The biosensor showed a linear increase in waveform as the concentration of Zika envelope in the serum increased, with a detection limit of 90.1 pM.
Review
Chemistry, Analytical
Pradakshina Sharma, Shariq Suleman, Asim Farooqui, Wajid Ali, Jagriti Narang, Shweta J. Malode, Nagaraj P. Shetti
Summary: This article introduces the diagnosis strategies for Ebola virus and the use of advanced technologies such as artificial intelligence and the internet of medical things for disease diagnosis. Timely disease identification and treatment optimization are crucial for controlling infectious disease outbreaks.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Analytical
Liyuan Zou, Xiaofeng Liu, Yuan Zhou, Wenjing Mei, Qing Wang, Xiaohai Yang, Kemin Wang
Summary: A colorimetric and photothermal point-of-care testing (POCT) biosensor based on rolling circle amplification (RCA) was developed for sensitive detection of cancerous exosomes. The biosensor showed colorimetric and photothermal responses, which were recorded using an optical fiber amplifier and a forehead thermometer, respectively, enabling hand-held detection. The biosensor exhibited excellent specificity and resistance to environmental interference.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Biophysics
Haotian Liang, Xuegang Wang, Fajun Li, Yinong Xie, Jiaqing Shen, Xueqin Wang, Yuqian Huang, Shaowei Lin, Junjie Chen, Lijian Zhang, Bingliang Jiang, Jinchun Xing, Jinfeng Zhu
Summary: Researchers have developed a label-free biosensor based on wafer-scale plasmonic meta-surfaces and a flexible spectral methodology for the rapid and precise diagnosis of early prostate cancer. This method has a diagnostic sensitivity of 92.3%, significantly higher than conventional PSA tests, and can be used for the detection of exosomes in serum.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Nanoscience & Nanotechnology
Zhe Zhang, Heng Jiang, Shen Jiang, Tuo Dong, Xiaotong Wang, Yunpeng Wang, Yang Li
Summary: Conventional detection methods are inadequate for early and rapid detection of monkeypox virus (MPXV) infection due to their complex pretreatment, time consumption, and complex operation. This study proposes a method based on surface-enhanced Raman spectroscopy (SERS) to capture the characteristic fingerprints of MPXV genome and antigenic proteins without the need for specific probes. The detection limit of the method is 100 copies/mL, with good reproducibility and signal-to-noise ratio. Principal component analysis (PCA) can identify SERS spectra of four different MPXV proteins in serum. Therefore, this rapid detection method has significant application prospects for controlling the current monkeypox outbreak and responding to future outbreaks.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Review
Chemistry, Analytical
Ghasem Ebrahimi, Parvin Samadi Pakchin, Amir Shamloo, Ali Mota, Miguel de la Guardia, Hossein Omidian, Yadollah Omidi
Summary: The integration of microfluidics with electrochemical analysis has led to the development of single miniaturized detection systems with precise control of sample volume and multi-analyte detection capability. Microfluidic electrochemical sensing devices (MESDs) offer several advantages, including automated sample preparation and detection, low sample requirement, and the ability to detect multiple analytes. Label-free MESDs allow for affordable real-time detection and can be used for point-of-care diagnosis and environmental analysis.
Article
Chemistry, Multidisciplinary
David S. Kinnamon, Jacob T. Heggestad, Jason Liu, Thu Nguyen, Vo Ly, Angus M. Hucknall, Cassio M. Fontes, Rhett J. Britton, Jian-Piao Cai, Jasper Fuk-Woo Chan, Kwok-Yung Yuen, Thuy Le, Ashutosh Chilkoti
Summary: We developed a self-contained immunoassay platform that can be used in field settings with varied temperature, humidity, and blood samples. The platform has high sensitivity and accuracy in detecting analyte concentration, and a portable detection device. We also improved the microfluidic cassette for better usability and faster results. By using this platform, we successfully conducted a rapid diagnostic test for talaromycosis infection in patients, showing comparable sensitivity and specificity to laboratory tests.
Article
Chemistry, Analytical
Philip R. Miller, Ryan D. Boehm, Shelby A. Skoog, Thayne L. Edwards, Mark Rodriguez, Susan Brozik, Igal Brener, Thomas Byrd, Justin T. Baca, Carlee Ashley, Roger J. Narayan, Ronen Polsky
Editorial Material
Emergency Medicine
J. K. Femling, J. T. Baca
ANNALS OF EMERGENCY MEDICINE
(2016)
Letter
Radiology, Nuclear Medicine & Medical Imaging
Alex Neuwelt, Jens Langsjoen, Thomas Byrd, Stephen Eberhardt, Gary Mlady, Justin Baca, Therese Bocklage, Cathleen Martinez, Jonathan Orner, Marisa Rivera, Laurel O. Sillerud
JOURNAL OF MAGNETIC RESONANCE IMAGING
(2017)
Article
Biochemical Research Methods
Bao Quoc Tran, Philip R. Miller, Robert M. Taylor, Gabrielle Boyd, Phillip M. Mach, C. Nicole Rosenzweig, Justin T. Baca, Ronen Polsky, Trevor Glaros
JOURNAL OF PROTEOME RESEARCH
(2018)
Article
Veterinary Sciences
Robert M. Taylor, Philip R. Miller, Parwana Ebrahimi, Ronen Polsky, Justin T. Baca
LABORATORY ANIMALS
(2018)
Article
Chemistry, Analytical
Justin T. Baca, David N. Finegold, Sanford A. Asher
Article
Emergency Medicine
Leana S. Wen, Justin T. Baca, Patricia O'Malley, Kriti Bhatia, David Peak, J. Kimo Takayesu
CANADIAN JOURNAL OF EMERGENCY MEDICINE
(2013)
Article
Chemistry, Analytical
Shane O'Sullivan, Zulfiqur Ali, Xiaoyi Jiang, Reza Abdolvand, M. Selim Unlu, Hugo Placido da Silva, Justin T. Baca, Brian Kim, Simon Scott, Mohammed Imran Sajid, Sina Moradian, Hakhamanesh Mansoorzare, Andreas Holzinger
Article
Engineering, Electrical & Electronic
Robert M. Taylor, Dilendra Maharjan, Fernando Moreu, Justin T. Baca
MICROSYSTEM TECHNOLOGIES-MICRO-AND NANOSYSTEMS-INFORMATION STORAGE AND PROCESSING SYSTEMS
(2020)
Letter
Respiratory System
Justin T. Baca, Nicholas F. Parchim, Jillian Kotulski, Jon Femling, Robert M. Taylor, Silas Bussmann, Lionel Candelaria, Tatsuya Norii, Michele Moyer, Rena Laliberte, Shivan Patel, Samantha Odeesho, Richard Nowak
JOURNAL OF AEROSOL MEDICINE AND PULMONARY DRUG DELIVERY
(2022)
Article
Biochemistry & Molecular Biology
Robert M. Taylor, Justin T. Baca
Summary: The study suggests that dermal interstitial fluid (ISF) may serve as a surrogate for blood in clinical settings for detecting ketone levels. A microneedle-based technology has been developed to extract ISF, enabling the development of wearable sensors for rapid ketosis detection.
Article
Biochemical Research Methods
Robert M. Taylor, Abdul-Mehdi S. Ali, Yiliang Zhu, Alicia M. Bolt, Justin T. Baca
Summary: This article introduces a minimally invasive technique for the extraction of interstitial fluid, which can be used for multiple extractions within weeks in animals, reducing the number of animals needed. The study also demonstrates the application of this technique for simultaneous quantification of different elements.
METHODS AND PROTOCOLS
(2022)
Article
Materials Science, Multidisciplinary
Philip R. Miller, Matthew Moorman, Ryan D. Boehm, Steven Wolfley, Victor Chavez, Justin T. Baca, Carlee Ashley, Igal Brener, Roger J. Narayan, Ronen Polsku
Article
Biology
Philip R. Miller, Robert M. Taylor, Bao Quoc Tran, Gabrielle Boyd, Trevor Glaros, Victor H. Chavez, Raga Krishnakumar, Anupama Sinha, Kunal Poorey, Kelly P. Williams, Steven S. Branda, Justin T. Baca, Ronen Polsky
COMMUNICATIONS BIOLOGY
(2018)