Article
Immunology
Chloe M. Doyle, Erica E. Vine, Kirstie M. Bertram, Heeva Baharlou, Jake W. Rhodes, Suat Dervish, Martijn P. Gosselink, Angelina Di Re, Geoffrey P. Collins, Faizur Reza, James W. T. Toh, Nimalan Pathma-Nathan, Golo Ahlenstiel, Grahame Ctercteko, Anthony L. Cunningham, Andrew N. Harman, Scott N. Byrne
Summary: This study discusses the importance of isolating and analyzing mononuclear phagocytes from fresh human tissue to understand their role in the immune system and diseases. The challenges of freeing these cells from tissue, including susceptibility to enzymatic cleavage and cell death, as well as immunological activation/maturation during the extraction process, are highlighted. The methods for anatomical separation of mucosa and submucosa, enzyme digestion optimization, and antibody clone selection for flow cytometry analysis are described, along with the ability to sort functional, immature intestinal dendritic cells for further functional assays.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Immunology
Yiwen Liu, Yan Gong, Gaosi Xu
Summary: Although the multiple hits theory is widely accepted in IgA nephropathy, increasing evidence suggests that the mononuclear/macrophage system plays important roles in disease progression, but the exact mechanism is unclear.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Gastroenterology & Hepatology
Duan-Rui Qiao, Guan-Yue Shan, Shuai Wang, Jun-Ya Cheng, Wei-Qun Yan, Hai-Jun Li
Summary: This article outlines the impact of hepatocellular carcinoma (HCC) on the mononuclear phagocyte system (MPS). It elaborates on how HCC reshapes MPS cell phenotype and the production of associated cytokines, as well as characterizes the function and impairment of the MPS in HCC.
WORLD JOURNAL OF GASTROENTEROLOGY
(2022)
Article
Chemistry, Multidisciplinary
Chang Liu, Yanfeng Zhou, Daoxia Guo, Yan Huang, Xiaoyuan Ji, Qian Li, Nan Chen, Chunhai Fan, Haiyun Song
Summary: Immunometabolic nanoparticles are developed to reshape intratumoral mononuclear phagocytes and enhance immune therapy against cancer.
Review
Biochemistry & Molecular Biology
Xu Shi, Hu Zhao, Yafei Kang, Xinyi Dong, Caiqian Yu, Qinying Xie, Yonggang Duan, Aihua Liao, Donghui Huang
Summary: This article outlines the role of mononuclear macrophages in the immune balance of the testes and epididymis and aims to understand the inner immune regulation mechanism. The review concludes that MPS exhibits a precise balance in the male reproductive system, maintaining immune tolerance and defense against pathogens.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Clinical Neurology
Ziqing Zhang, Yunpeng Liu, Yiqi Liu, Wang Yang
Summary: Ischemic stroke (IS) leads to an inflammatory response involving the mononuclear phagocyte system (MPS), including activated microglia and monocytes/macrophages, which play diverse roles in various phases of IS, such as recruitment, polarization, phagocytosis, angiogenesis, and interaction with other types of cells. Understanding the functions of the MPS could provide insights for predicting prognosis and developing novel therapeutic strategies for IS.
Article
Biochemistry & Molecular Biology
Annika Droste, Gustavo Chaves, Stefan Stein, Annette Trzmiel, Matthias Schweizer, Hubert Karl, Boris Musset
Summary: The respiratory burst of phagocytes relies on the production of reactive oxygen species (ROS) by NADPH oxidase (NOX2) and the voltage-gated proton channel (HV1). The study shows that human PMN and PLB 985 generate ROS through a common mechanism involving NOX2 and HV1. Results suggest an essential interconnection between HV1 and NOX2 during the respiratory burst.
Review
Cell Biology
Martyna Cieslik, Krzysztof Bryniarski, Katarzyna Nazimek
Summary: Currently, extracellular vesicles (EVs) are considered promising for cell-free therapies, but their effectiveness is limited by rapid clearance by the mononuclear phagocyte system (MPS). Strategies to avoid EV elimination are necessary for their clinical application. On the other hand, dysfunctional MPS is associated with immune-related pathologies, which could potentially be reversed by EVs through modulating macrophage phenotype or regulating antigen presentation by dendritic cells. Targeting EVs to MPS macrophages for replication and repackaging of their molecules into new vesicle subtype can enable specific targeting to desired cells. Further research on MPS-EV interactions is needed for the therapeutic use of EVs.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Medicine, Research & Experimental
Pu Reun Roh, Sung Min Kim, Byung-Yoon Kang, Kyoung Do Mun, Jong Geun Park, Min Woo Kang, Wonhee Hur, Ji Won Han, Heechul Nam, Seung Kew Yoon, Pil Soo Sung
Summary: TAF exhibits anti-inflammatory effects in NASH livers by attenuating AKT phosphorylation in intrahepatic activated MPs, suggesting it as a potential therapeutic option for NASH.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Immunology
Hyun Yang, Ji Won Han, Jae Jun Lee, Ahlim Lee, Sung Woo Cho, Pu Reun Rho, Min-Woo Kang, Jeong Won Jang, Eun Sun Jung, Jong Young Choi, Pil Soo Sung, Si Hyun Bae
Summary: This study investigated the phenotypes and clinical implications of liver-infiltrating immune cells in idiosyncratic drug-induced liver injury (DILI). The results showed that activated CD8+ T cells and mononuclear phagocytes are associated with drug-induced liver injury. Therefore, steroids are suggested as a potential treatment option for idiosyncratic DILI.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Oncology
Jingqi Liu, Chengyu Fang, Xinyan Jin, Guo Tian, Zhongxia Sun, Lijie Hong, Jinhua Pan, Xinhua Chen, Jun Zhao, Hongcui Cao, Tianan Jiang
Summary: Preclinical studies have shown that nanosecond pulsed electric field (nsPEF) ablation can be a safe and effective treatment for patients with unresectable liver cancer. The activation of antitumor immunity by nsPEF can potentially prevent tumor recurrence. A prospective clinical trial was conducted to evaluate the safety and efficacy of nsPEF ablation in patients with unresectable liver cancer.
MOLECULAR ONCOLOGY
(2023)
Article
Immunology
Kipyo Kim, Yun-Gyeong Kim, Su Woong Jung, Yang Gyun Kim, Sang-Ho Lee, Seung-Hae Kwon, Ju-Young Moon
Summary: This study utilized optical tissue clearing combined with multiphoton microscopy and light sheet fluorescence microscopy to evaluate and visualize the morphological and functional alterations of renal mononuclear phagocytes (MNPs) in acute kidney injury (AKI). The findings showed that CD11c+ cells increased in number and underwent morphological changes in AKI, accompanied by increased expression of MHC class II and CD86 on CD11c-YFP+ cells. The NLRP3 inhibitor MCC950 attenuated the activation of CD11c+ cells and improved renal function in AKI.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Qingxiao Song, Xiaoning Wang, Xiwei Wu, Tae Hyuk Kang, Hanjun Qin, Dongchang Zhao, Robert R. Jenq, Marcel R. M. van den Brink, Arthur D. Riggs, Paul J. Martin, Yuan-Zhong Chen, Defu Zeng
Summary: The pathogenesis of SR-Gut-aGVHD involves the expansion of Th/Tc22 cells causing dysbiosis and reduction of Th/Tc1 cells leading to depletion of CX3CR1(hi) mononuclear phagocytes. Potential therapeutic strategies for SR-Gut-aGVHD include IL-22 antagonists and IFN-γ agonists.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Sarah E. Garnish, Katherine R. Martin, Maria Kauppi, Victoria E. Jackson, Rebecca Ambrose, Vik Ven Eng, Shene Chiou, Yanxiang Meng, Daniel Frank, Emma C. Tovey Crutchfield, Komal M. Patel, Annette V. Jacobsen, Georgia K. Atkin-Smith, Ladina Di Rago, Marcel Doerflinger, Christopher R. Horne, Cathrine Hall, Samuel N. Young, Matthew Cook, Vicki Athanasopoulos, Carola G. Vinuesa, Kate E. Lawlor, Ian P. Wicks, Gregor Ebert, Ashley P. Ng, Charlotte A. Slade, Jaclyn S. Pearson, Andre L. Samson, John Silke, James M. Murphy, Joanne M. Hildebrand
Summary: The MLKL gene mutation in human cells and the Mlkl mutation in mouse cells can affect the cell death pathway. The Mlkl mutation enhances necroptotic function, and the S131P substitution impairs the return to homeostasis after systemic challenge.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Sarah E. Garnish, Katherine R. Martin, Maria Kauppi, Victoria E. Jackson, Rebecca Ambrose, Vik Ven Eng, Shene Chiou, Yanxiang Meng, Daniel Frank, Emma C. Tovey Crutchfield, Komal M. Patel, Annette V. Jacobsen, Georgia K. Atkin-Smith, Ladina Di Rago, Marcel Doerflinger, Christopher R. Horne, Cathrine Hall, Samuel N. Young, Matthew Cook, Vicki Athanasopoulos, Carola G. Vinuesa, Kate E. Lawlor, Ian P. Wicks, Gregor Ebert, Ashley P. Ng, Charlotte A. Slade, Jaclyn S. Pearson, Andre L. Samson, John Silke, James M. Murphy, Joanne M. Hildebrand
Summary: The S131P mutation in the MLKL gene affects the process of necroptosis, enhances necroptotic function, and has an impact on the recovery of the body after systemic challenge. In mouse cells, the Mlkl(S131P) mutation leads to a dosage-dependent reduction in TNF-induced necroptosis, but increased sensitivity to IFN-beta induced cell death.
NATURE COMMUNICATIONS
(2023)