Article
Pharmacology & Pharmacy
Qiong Chen, Ting Zhou, Jun-jie Yuan, Xiao-yi Xiong, Xue-hui Liu, Zong-ming Qiu, Lin-lin Hu, Hui Lu, Qian He, Chang Liu, Qing-wu Yang
Summary: This study investigated the metabolic alterations during ischemia-reperfusion injury using metabolomic analysis. A total of 82 differentially expressed metabolites were identified, with some significantly elevated in the reperfusion group. Metabolomics pathway analysis revealed the importance of glycerophospholipid metabolism, linoleic acid metabolism, pyrimidine metabolism, and galactose metabolism in the response to cerebral ischemia-reperfusion. This study provides important insights into the metabolic changes induced by cerebral ischemia-reperfusion.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Cell Biology
Chandranayaka Siddaiah, Anil Kumar BM, Saligrama Adavigowda Deepak, Syed Salman Lateef, Saurabh Nagpal, Kanchugarakoppal S. Rangappa, Chakrabhavi D. Mohan, Shobith Rangappa, Madan S. Kumar, Minaxi Sharma, Vijai Kumar Gupta
Summary: This study focused on metabolite profiling and identification from the bark of Alangium salviifolium by using organic and aqueous solvents. Eighty-one secondary metabolites with therapeutic potential were identified, showcasing the therapeutic value of this plant.
Article
Plant Sciences
Sang Suk Kim, Hyun-Jin Kim, Kyung Jin Park, Seok Beom Kang, YoSup Park, Seong-Gab Han, Misun Kim, Yeong Hun Song, Dong-Shin Kim
Summary: This study analyzed the changes in chemical composition during the fruit development process of Citrus unshiu, revealing the trends in total phenolic content, total carotenoid content, antioxidant activity, and metabolite profiles at different stages, providing important information for understanding these changes.
Article
Biochemistry & Molecular Biology
Daniela Magalhaes Nolasco, Michele P. R. Mendes, Luiz Paulo de Aguiar Marciano, Luiz Filipe Costa, Adriana Nori De Macedo, Isarita Martins Sakakibara, Alessandra Cristina Pupin Silverio, Maria Jose N. Paiva, Leiliane C. Andre
Summary: Pesticides are chemical products used for pest control, but their increased use has led to increased risks to human health and the environment due to exposure. This study used metabolomics to analyze the metabolic profile of occupationally exposed individuals to pesticides. The analysis identified potential biomarkers and revealed alterations in lipid and amino acid metabolism. The study highlights the importance of metabolomics in understanding complex biological responses.
Article
Medical Laboratory Technology
Zheng Zhong, Yukai Huang, Qidang Huang, Shaoling Zheng, Zhixiang Huang, Weiming Deng, Tianwang Li
Summary: This study investigated the serum metabolic profiling of gout patients compared to healthy controls using UPLC-Q-TOF/MS, revealing significant differences in metabolites between the two groups. Bilirubin was identified as a potential biomarker for gout, and primary bile acid biosynthesis was suggested as a novel metabolic pathway.
CLINICA CHIMICA ACTA
(2021)
Article
Toxicology
Zheng Xiang, Jieying Qiu, Xiaoying He, Xinwei Yu
Summary: This study investigated the metabolic profile and toxicokinetics of gelsenicine in rats using UPLC and mass spectrometry. The results revealed that gelsenicine is mainly metabolized in the liver and excreted through urine and bile. This study provides important information for the risk assessment and forensic identification of gelsenicine.
ARCHIVES OF TOXICOLOGY
(2022)
Article
Food Science & Technology
Dong-Shin Kim, Sun Lee, Suk Man Park, Su Hyun Yun, Han-Seung Gab, Sang Suk Kim, Hyun-Jin Kim
Summary: The study analyzed 10 citrus varieties to evaluate their chemical profiles and antioxidant activities, finding significant variations in content of sugars, organic acids, flavonoids, limonoids, and carotenoids among different varieties. These differences led to variations in antioxidant capacity and quality parameters. The study provides valuable information for understanding the relationship between citrus quality and metabolite profiles, which can be useful for the development and improvement of new citrus varieties.
Article
Chemistry, Analytical
Jiajin Huang, Lijun Ding, Wenyue Tian, Hui Zhi, Jing Chen, Liangcai Wu, Likang Wang, Juntao Xie, Jingyan Bai, Hongxia Fan, Suqing Zhao, Kun Zhang, Junxia Zheng
Summary: In this study, the polyphenolic profile of Mesona chinensis Benth (MCB) was determined using UPLC-Q-TOF-MS/MS, identifying a total of 57 compounds including 45 polyphenols. Among them, 23 phytochemicals were identified in MCB for the first time. The antioxidant activities were found to be positively correlated with the polyphenolic levels, with MCB produced in Guangdong Raoping showing the best antioxidant activity and strongest inhibitory effect on alpha-glucosidase.
MICROCHEMICAL JOURNAL
(2021)
Article
Plant Sciences
Noga Sikron-Persi, Gila Granot, Albert Batushansky, David Toubiana, Gideon Grafi, Aaron Fait
Summary: A study on the petioles of perennial desert shrub Zygophyllum dumosum Boiss revealed a consistent seasonal pattern in petiole metabolism, involving organic acids, polyols, phenylpropanoids, sulfate conjugates, and piperazines. The metabolic changes were primarily related to the plant's development stage and its interaction with the environment, rather than specific environmental conditions.
Article
Horticulture
Dong-Shin Kim, Kee-Jai Park, Jeong Hee Choi, Jeong-Ho Lim, Hyun-Jin Kim
Summary: This study investigated the changes in quality-related metabolites of strawberries at different maturities during postharvest storage. The results showed that the surface color of strawberries changed to red, SSC/TA ratio increased, and firmness slightly increased during storage. Moreover, the metabolite profiles related to strawberry quality also changed, including sugars, acidic compounds, lipids, flavonoids, phenolic acid derivatives, and tannins. The knowledge gained from this study helps in understanding the changes in quality-related metabolites of strawberries according to maturity and postharvest storage.
SCIENTIA HORTICULTURAE
(2023)
Article
Biochemistry & Molecular Biology
Mohamed A. Farag, Tarik A. Mohamed, Enas A. El-Hawary, Amr Abdelwareth
Summary: Luwak coffee, known as civet coffee, is a precious and exotic type of coffee. This study used a comparative metabolomics approach to analyze the secondary and aroma metabolites of Luwak coffee and other coffee products. The results showed that Luwak coffee is derived from C. arabica and identified several new markers. Aroma analysis revealed higher levels of guaiacol derivatives, pyrazines, and furans in roasted Luwak coffee compared to roasted C. arabica. Quantification using NMR found lower levels of alkaloids in Luwak coffee compared to C. arabica.
Article
Biochemistry & Molecular Biology
Dong-Sik Yang, Zhentian Lei, Mohamed Bedair, Lloyd W. Sumner
Summary: The optimized SPME-GC-MS method was used to quantify and qualify volatile metabolites in alfalfa glandular trichomes, leading to the detection of tissue-specific volatile metabolites. HCA and PCA analysis demonstrated that the method effectively differentiated metabolic profiles of different tissues.
Article
Agriculture, Multidisciplinary
Mingjin Li, Yunzhi Zhang, Jiawei Yan, Fengjiao Ding, Chunmei Chen, Sitong Zhong, Minxuan Li, Yanyu Zhu, Panpan Yue, Pengchun Li, Shijun You, Shan Jin
Summary: Beauty tea was made from fresh leaves of Camellia sinensis Jinxuan cultivar, which were punctured by tea green leafhoppers to different extents. The study identified differential metabolites related to tea polyphenols, lipids, and saccharides in the fresh leaves and dry beauty tea. The quality of beauty tea was influenced by the levels of leafhopper puncturing through the presence of specific characteristic volatiles.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Chemistry, Applied
Ajay Anand, Anusha Komati, Kumar Katragunta, Hussain Shaik, Narendra Kumar Nagendla, Madhusudana Kuncha, Mohana Krishna Reddy Mudiam, Katragadda Suresh Babu, Ashok Kumar Tiwari
Summary: Boiled rhizome of Nymphaea nouchali shows significant antihyperglycemic and antioxidative stress potentials, making it a promising food material for managing hyperglycemia and oxidative stress.
Article
Integrative & Complementary Medicine
Fengge Yang, Sihao Zhang, Danmei Tian, Guirong Zhou, Xiyang Tang, Xinglong Miao, Yi He, Xinsheng Yao, Jinshan Tang
Summary: This study employed UPLC-Q/TOF-MS combined with network pharmacology to characterize the phytochemical components and metabolites of CKF and elucidate its underlying mechanism. The study identified 150 components in the CKF formula, including new substances from Cicadae Periostracum and Cuscutae Semen. Additionally, the study detected 149 xenobiotics and elucidated the metabolic pathways of resin glycosides for the first time. Network pharmacology and molecular docking analyses revealed potential targets and components responsible for the beneficial effects of CKF in treating IBS. These findings provide a comprehensive understanding of the CKF formula and its potential in treating IBS, facilitating its broader application in medicine.
CHINESE JOURNAL OF NATURAL MEDICINES
(2023)
Article
Biochemistry & Molecular Biology
Kyoungwon Cho, You-Ran Jang, Sun-Hyung Lim, Susan B. Altenbach, Yong Q. Gu, Annamaria Simon-Buss, Jong-Yeol Lee
Summary: This study utilized advanced techniques to analyze the composition of LMW-GS in wheat, addressing the challenge of determining the contribution of each LMW-GS to flour quality. It provided important information for wheat breeding and screening desirable LMW-GS alleles for quality improvement.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Sun-Hyung Lim, Da-Hye Kim, Jae-A. Jung, Jong-Yeol Lee
Summary: The study found that the alternative splicing of CmbHLH2 plays a key role in regulating the color changes of chrysanthemum ray florets. The shorter splicing variant CmbHLH2(Short) failed to activate anthocyanin biosynthetic genes and induce pigment accumulation, while the full-length form CmbHLH2(Full) promoted these processes when co-expressed with CmMYB6.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Food Science & Technology
Seung-A Baek, So Yeon Kim, Young Jin Park, Tae Jin Kim, Sun-Hyung Lim, Sang Un Park, Jae Kwang Kim
Summary: GC x GC-TOFMS and GC-TOFMS systems were compared for polar metabolite profiling of rice seeds, with GC x GC-TOFMS showing lower detection limits and better separation of overlapped peaks. Principal component analysis revealed unique metabolic profiles in Heugdaegu rice cultivar, suggesting potential applications in breeding programs for improving rice seed quality.
APPLIED BIOLOGICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Tae Jin Kim, So Yeon Kim, Young Jin Park, Sun-Hyung Lim, Sun-Hwa Ha, Sang Un Park, Bumkyu Lee, Jae Kwang Kim
Summary: The study conducted comprehensive profiling of primary and secondary metabolites to investigate the metabolic differences associated with color formation in pigmented rice, identifying high levels of flavonoids in black and red rice and high levels of terpenoids in black rice. Black rice was found to be rich in carbon-based secondary metabolites compared to other rice cultivars, with relatively low levels of primary metabolites.
Article
Biochemistry & Molecular Biology
Da-Hye Kim, Jundae Lee, JuHee Rhee, Jong-Yeol Lee, Sun-Hyung Lim
Summary: The study identified an allele of RsMYB1, named RsMYB1(Short), in white radish cultivars, resulting in a truncated protein that lost the ability to regulate anthocyanin biosynthesis. This frame shift mutation of RsMYB1 plays a key role in anthocyanin biosynthesis in the radish taproot.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Su-Bin Lee, Yu-Jeong Yang, Sun-Hyung Lim, Yong Q. Gu, Jong-Yeol Lee
Summary: The study established an optimized RP-UPLC method for the identification of wheat HMW-GS subunits, which was successfully applied to screen for favorable wheat lines with high processing quality of flour.
Article
Plant Sciences
Da-Hye Kim, JuHee Yang, Sun-Hwa Ha, Jae Kwang Kim, Jong-Yeol Lee, Sun-Hyung Lim
Summary: The study characterized the role of the R2R3 MYB gene OsKala3 in rice pericarp pigmentation, demonstrating its physical interaction with OsKala4 and the correlation between the number of repeats within the OsKala3 promoter region and anthocyanin biosynthesis in rice pericarp. These findings provide insights into the molecular basis of anthocyanin biosynthesis in rice pericarp and potential implications for breeding programs.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
JiYeon Kim, Da-Hye Kim, Jong-Yeol Lee, Sun-Hyung Lim
Summary: BrMYBL2.1-G is identified as a key negative regulator of anthocyanin biosynthesis, inhibiting the transcriptional activity of anthocyanin biosynthetic genes and blocking the activity of the MBW complex. In contrast, BrMYBL2.1-P cannot exert this inhibitory effect, resulting in increased anthocyanin accumulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Ye Sol Jeong, Heebak Choi, Jae Kwang Kim, Seung-A Baek, Min-Kyoung You, Dongho Lee, Sun-Hyung Lim, Sun-Hwa Ha
Summary: OsMYBR22, an R1type-MYB transcription factor in rice, plays a positive regulatory role in the accumulation of chlorophyll and carotenoids. Overexpression of OsMYBR22 leads to everlasting green seeds and leaves in rice. This transcription factor is crucial for the regulation of chlorophyll biosynthesis and degradation.
METABOLIC ENGINEERING
(2022)
Article
Plant Sciences
Sun-Hyung Lim, Da-Hye Kim, Myeong-Cheoul Cho, Jong-Yeol Lee
Summary: The CaAN2 gene from purple chili pepper was evaluated as a visible selection marker for transgenic plants, inducing pigment accumulation in tobacco leaves without the need for herbicides and bHLH proteins. This alternative selection system based on anthocyanin accumulation is stable and effective for transgenic plant transformation.
Article
Plant Sciences
Sun-Hyung Lim, Da-Hye Kim, Jae-A Jung, Nam-In Hyung, YeoJin Youn, Jong-Yeol Lee
Summary: Suppressing the CmDFR gene through post-transcriptional gene silencing enhances flavonoid content and antioxidant activity in ray florets of chrysanthemums.
Article
Biochemistry & Molecular Biology
Sun-Hyung Lim, Da-Hye Kim, Jong-Yeol Lee
Summary: The WD40 gene RsTTG1 from Raphanus sativus was characterized and found to play a crucial role in pigmentation and trichome development. RsTTG1 interacts with RsTT8 and moves from the cytoplasm to the nucleus in the presence of RsTT8. Co-expression of RsTTG1 with RsMYB1 and RsTT8 activates the promoters of anthocyanin biosynthesis genes. These findings highlight the importance of RsTTG1 as a functional homolog of AtTTG1.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agronomy
Sewon Kim, Jae-Ryeong Sim, Yong Q. Gu, Susan B. Altenbach, Sandra Denery-Papini, Florence Pineau, Olivier Tranquet, Yu-Jeong Yang, Eun Ji Park, Sun-Hyung Lim, Chon-Sik Kang, Changhyun Choi, Jong-Yeol Lee
Summary: In this study, 665 wheat germplasm samples were screened, and 11 wheat lines missing the 1D-encoded omega-5 gliadins were identified. Quantification and analysis revealed that these lines not only lacked the omega-5 gliadin genes, but also showed reduced expression of omega-1,2 gliadins. The identification of these wheat lines missing the omega-5 gliadins is important for breeding efforts to reduce the immunogenic potential of wheat flour for wheat allergy patients.
THEORETICAL AND APPLIED GENETICS
(2023)
Article
Plant Sciences
Da-Hye Kim, Sun-Hyung Lim, Jong-Yeol Lee
Summary: Radish plants exhibit varied root colors due to the accumulation of chlorophylls and anthocyanins compounds. The role of NADPH:protochlorophyllide oxidoreductases (PORs) in radish roots were examined in this study. The abundantly expressed RsPORB gene was found to be correlated with chlorophyll content. Deletion and insertion mutations in the RsPORB promoter were found to affect its expression level. RsPORB was identified as a key gene underlying chlorophyll biosynthesis in non-foliar tissues.
Article
Biotechnology & Applied Microbiology
Ye Sol Jeong, Hyung-Keun Ku, Young-Joo Jung, Jae Kwang Kim, Kyoung Bok Lee, Ju-Kon Kim, Sun-Hyung Lim, Dongho Lee, Sun-Hwa Ha
Summary: Coordinating the coexpression of carotenoid biosynthetic genes using synthetic sequences allows the production of multiple carotenoids in transgenic rice seeds with various colors.
METABOLIC ENGINEERING COMMUNICATIONS
(2021)