期刊
MOLECULES
卷 15, 期 6, 页码 4227-4241出版社
MDPI
DOI: 10.3390/molecules15064227
关键词
Cordyceps; carbohydrates; polysaccharides; pressurized liquid extraction (PLE); GC-MS
资金
- Science and Technology Development Found of Macau [028/2006/A2]
- University of Macau [UL015A/09-Y2]
Free and polymeric carbohydrates in Cordyceps, a valued edible mushroom and well-known traditional Chinese medicine, were determined using stepwise pressurized liquid extraction (PLE) extraction and GC-MS. Based on the optimized PLE conditions, acid hydrolysis and derivatization, ten monosaccharides, namely rhamnose, ribose, arabinose, xylose, mannose, glucose, galactose, mannitol, fructose and sorbose in 13 samples of natural and cultured Cordyceps were qualitatively and quantitatively analyzed and compared with myo-inositol hexaacetate as internal standard. The results showed that natural C. sinensis contained more than 7.99% free mannitol and a small amount of glucose, while its polysaccharides were usually composed of mannose, glucose and galactose with a molar ratio of 1.00:16.61 similar to 3.82:1.60 similar to 1.28. However, mannitol in cultured C. sinensis and cultured C. militaris were less than 5.83%, and free glucose was only detected in a few samples, while their polysaccharides were mainly composed of mannose, glucose and galactose with molar ratios of 1.00:3.01 similar to 1.09:3.30 similar to 1.05 and 1.00:2.86 similar to 1.28:1.07 similar to 0.78, respectively. Natural and cultured Cordyceps could be discriminated by hierarchical clustering analysis based on its free carbohydrate contents.
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