Article
Forestry
Katarzyna Nawrot-Chorabik, Malgorzata Sulkowska, Malgorzata Osmenda, Vasyl Mohytych, Ewa Surowka, Dariusz Latowski
Summary: Fraxinus excelsior L. is threatened by various environmental factors, including fungi, heavy metal accumulation, and salinity. This study investigated the impact of these factors on European ash using in vitro techniques, revealing the strong pathogenic potential of fungi and the high toxicity of heavy metals.
Article
Forestry
Tadeusz Kowalski, Piotr Bilanski, Bartlomiej Grad
Summary: This study investigates the occurrence and pathogenicity of A. hystrix on Acer pseudoplatanus and Fraxinus excelsior at forest sites in Poland. The results indicate that A. hystrix is pathogenic to A. pseudoplatanus but not to F. excelsior.
Article
Forestry
Tadeusz Kowalski, Piotr Bilanski
Summary: In 2017, a study on fungal communities in previous year's leaf petioles of Fraxinus excelsior found in litter in five ash stands in southern Poland was conducted. Fungi were identified through isolation and in situ inventory, revealing the diversity of harmful fungi affecting Fraxinus. The study discussed the possible contribution of saprotrophic fungi in limiting the expansion of Hymenoscyphus fraxineus in ash leaf petioles, which could lead to a reduction in the inoculum of ash dieback causal agent.
Article
Plant Sciences
Anna Hebda, Aleksandra Liszka, Piotr Zglobicki, Katarzyna Nawrot-Chorabik, Jan J. Lyczakowski
Summary: This study presents the first successful genetic transformation of European ash callus tissue, providing a selection process for stable transgenic callus lines to study gene function and design breeding strategies for resistant trees. The use of Agrobacterium tumefaciens to transform callus tissue from F. excelsior embryos was key in demonstrating the stable expression of transgenes. The transformation techniques described in this paper are expected to facilitate rapid progress in uncovering the molecular basis of ash dieback and validating gene targets for resistance to Hymenoscyphus fraxineus pathogenicity.
Article
Forestry
Corrie Lynne Madsen, Chatchai Kosawang, Iben Margrete Thomsen, Lars Norgaard Hansen, Lene R. Nielsen, Erik Dahl Kjaer
Summary: Ash dieback, caused by the fungus Hymenoscyphus fraxineus, has been spreading in Europe for over two decades, leading to decline in the health of ash trees. The disease progressed rapidly, with young stands experiencing higher levels of symptoms, while even old stands eventually showed decreased health and growth. Despite natural infection pressure, a small percentage of trees showed resistance to the disease and honey fungus.
FOREST ECOLOGY AND MANAGEMENT
(2021)
Article
Forestry
Remigijus Bakys, Alfas Pliura, Gintare Bajerkeviciene, Adas Marciulynas, Diana Marciulyniene, Jurate Lynikiene, Audrius Menkis
Summary: Ash dieback caused by the alien fungus Hymenoscyphus fraxineus has led to the decline of European ash in Lithuania. However, recent inventories have found naturally regenerated ash stands. A study on these stands revealed that the fungal communities associated with ash were mainly composed of endophytic fungi, some of which may limit the infection rate or spread of H. fraxineus. The diversity of fungal taxa was found to be higher in natural ash habitats compared to planted trials, highlighting the importance of natural habitats in ash restoration programs.
Article
Microbiology
Tadeusz Kowalski, Piotr Bilanski
Summary: This study aimed to find fungi with potential biocontrol capabilities against the ash dieback pathogen. Eighteen fungal taxa were identified and observed to have inhibitory effects on the pathogenic fungus H. fraxineus. The findings suggest that these fungi may contribute to reducing the spread of H. fraxineus.
Article
Forestry
Krzysztof Turczanski, Marta Belka, Rafal Kukawka, Maciej Spychalski, Marcin Smiglak
Summary: The study aimed to investigate the effect of using BTHWA on ash saplings. Results showed that the treatment reduced the size of necrotic phloem lesions caused by H. fraxineus. The findings suggest that triggering plant resistance by BTHWA can slow down or even completely stop the development of H. fraxineus in ash saplings.
Article
Forestry
Kateryna Davydenko, Yuriy Skrylnyk, Oleksandr Borysenko, Audrius Menkis, Natalia Vysotska, Valentyna Meshkova, Ake Olson, Malin Elfstrand, Rimvys Vasaitis
Summary: The study found that the expansion range of Emerald Ash Borer (EAB) in Ukraine is increasing, and European ash trees have stronger resistance to EAB but are more susceptible to dieback disease. The research suggests that it is necessary to inventory and map surviving European ash trees affected by both diseases, in order to carry out long-term restoration work and prevent the invasion of EAB into the EU.
Article
Biotechnology & Applied Microbiology
Malin Elfstrand, Jun Chen, Michelle Cleary, Sandra Halecker, Katarina Ihrmark, Magnus Karlsson, Kateryna Davydenko, Jan Stenlid, Marc Stadler, Mikael Brandstrom Durling
Summary: The genomes of Hymenoscyphus fraxineus and H. albidus exhibit a high level of synteny and identity, with the former showing more dispersed repeat content, particularly in transposable elements. While relatively few genes show signs of positive selection between the species, those associated with secondary metabolite gene families in H. fraxineus may play a role in its rapid establishment and displacement of H. albidus. The genomic signatures suggest that H. albidus may be less well equipped to adapt and thrive in a changing environment.
Article
Forestry
Anna-Katharina Eisen, Barbara Fussi, Branko Sikoparija, Susanne Jochner-Oette
Summary: This study investigates the factors affecting ash pollen transport through field experiments and finds that meteorology, phenological development, and plantation topography are associated with pollen dispersion. Trees outside the plantation contribute more pollen in low flowering years, while trees within the plantation contribute more in high pollen years. Pollen can be transported for distances greater than 400 meters, but the amount of pollen decreases significantly with increasing distance.
Article
Plant Sciences
Regina Becker, Kristina Ulrich, Undine Behrendt, Volker Schneck, Andreas Ulrich
Summary: Research has found that a bacterium called C2P003 can help resist the invasive pathogen Hymenoscyphus fraxineus, which causes dieback in European ash trees. This bacterium suppresses the infection by colonizing the ash leaves and affecting the bacterial microbiome, leading to improved plant health.
Article
Microbiology
Remigijus Bakys, Gintare Bajerkeviciene, Alfas Pliura, Adas Marciulynas, Diana Marciulyniene, Jurate Lynikiene, Valeriia Mishcherikova, Audrius Menkis
Summary: Fifty-nine fungal taxa isolated from re-emerging Fraxinus excelsior sites in Lithuania were tested in vitro against three strains of Hymenoscyphus fraxineus, with some fungi showing strong growth inhibition of the pathogen.
Article
Forestry
Lisa Buchner, Anna-Katharina Eisen, Branko Sikoparija, Susanne Jochner-Oette
Summary: This study investigates the impact of long-distance transport on pollen viability in fragmented populations of ash trees affected by ash dieback. The viability of ash pollen is crucial for the survival of the species. The experiments conducted in a climate chamber and with a volumetric pollen trap show that prevailing temperatures have a significant influence on pollen viability, with warmer conditions causing faster decline. Optimal storage temperature and germination medium were determined, and properly stored pollen can be used for breeding programs to support the survival of Fraxinus excelsior.
Article
Forestry
Trevor Fenning, Margaret O'Donnell, Katharine Preedy, Aurelia Bezanger, David Kenyon, Gustavo Lopez
Summary: This study established a set of standard tissue culture methods for propagation of ash trees and successfully produced a large number of rooted clonal plants. The findings are important for overcoming ash die-back disease.
ANNALS OF FOREST SCIENCE
(2022)
Article
Cell Biology
Christine Faulkner, Ji Zhou, Alexandre Evrard, Gildas Bourdais, Dan MacLean, Heidrun Haweker, Peter Eckes, Silke Robatzek
Article
Biology
Ram Krishna Shrestha, Pingtao Ding, Jonathan D. G. Jones, Dan MacLean
Article
Ecology
Mark McMullan, Maryam Rafiqi, Gemy Kaithakottil, Bernardo J. Clavijo, Lorelei Bilham, Elizabeth Orton, Lawrence Percival-Alwyn, Ben J. Ward, Anne Edwards, Diane G. O. Saunders, Gonzalo Garcia Accinelli, Jonathan Wright, Walter Verweij, Georgios Koutsovoulos, Kentaro Yoshida, Tsuyoshi Hosoya, Louisa Williamson, Philip Jennings, Renaud Ioos, Claude Husson, Ari M. Hietala, Adam Vivian-Smith, Halvor Solheim, Dan MaClean, Christine Fosker, Neil Hall, James K. M. Brown, David Swarbreck, Mark Blaxter, J. Allan Downie, Matthew D. Clark
NATURE ECOLOGY & EVOLUTION
(2018)
Article
Biochemical Research Methods
Ghanasyam Rallapalli, Pilar Corredor-Moreno, Edward Chalstrey, Martin Page, Daniel MacLean
BMC BIOINFORMATICS
(2019)
Article
Cell Biology
Gildas Bourdais, Deirdre H. McLachlan, Lydia M. Rickett, Ji Zhou, Agnieszka Siwoszek, Heidrun Haweker, Matthew Hartley, Hannah Kuhn, Richard J. Morris, Dan MacLean, Silke Robatzek
Article
Horticulture
Juan C. Diaz-Perez, Dan MacLean, Smiljana Goreta, Sarah Workman, Erick Smith, Harwinder Singh Sidhu, Gunawati Gunawan, Anthony Bateman, Jesus Bautista, William Lovett, Maja Jukic Spika, Gvozden Dumicic, Mira Radunic
Article
Biology
Juan Carlos De la Concepcion, Marina Franceschetti, Dan MacLean, Ryohei Terauchi, Sophien Kamoun, Mark J. Banfield
Article
Biotechnology & Applied Microbiology
Pingtao Ding, Bruno Pok Man Ngou, Oliver J. Furzer, Toshiyuki Sakai, Ram Krishna Shrestha, Dan MacLean, Jonathan D. G. Jones
PLANT BIOTECHNOLOGY JOURNAL
(2020)
Article
Biochemical Research Methods
Ruth Kristianingsih, Dan MacLean
Summary: Effector proteins of plant pathogens cause significant crop losses annually. Deep learning models based on Convolutional Neural Networks were developed to accurately identify these effectors, outperforming traditional detection software. The study found distinct N-terminal regions in various taxonomic groups that may serve as indicators for effector proteins, providing biological insights for classification.
BMC BIOINFORMATICS
(2021)
Article
Plant Sciences
Pingtao Ding, Toshiyuki Sakai, Ram Krishna Shrestha, Nicolas Manosalva Perez, Wenbin Guo, Bruno Pok Man Ngou, Shengbo He, Chang Liu, Xiaoqi Feng, Runxuan Zhang, Klaas Vandepoele, Dan MacLean, Jonathan D. G. Jones
Summary: This study combines RNA-seq and ATAC-seq to investigate the effects of cell-surface and intracellular receptor-mediated immunity activation on gene expression and chromatin accessibility. The results reveal overlapping and conserved transcriptional regulatory mechanisms between the two immune systems.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Biochemistry & Molecular Biology
Oliver J. Furzer, Volkan Cevik, Sebastian Fairhead, Kate Bailey, Amey Redkar, Christian Schudoma, Dan MacLean, Eric B. Holub, Jonathan D. G. Jones
Summary: In this study, we conducted a comprehensive analysis of the genome of Albugo candida. Our results showed a significant expansion of the CHxC effector class in the new assembly, which is mainly located in gene sparse regions. The findings of this study contribute to the understanding of the virulence and avirulence of A. candida on different hosts.
MOLECULAR PLANT-MICROBE INTERACTIONS
(2022)
Article
Biochemistry & Molecular Biology
Lida Derevnina, Mauricio P. Contreras, Hiroaki Adachi, Jessica Upson, Angel Vergara Cruces, Rongrong Xie, Jan Sklenar, Frank L. H. Menke, Sam T. Mugford, Dan MacLean, Wenbo Ma, Saskia Hogenhout, Aska Goverse, Abbas Maqbool, Chih-Hang Wu, Sophien Kamoun
Summary: Research has shown that plant NLR proteins can form receptor networks to provide cell death and immunity. Pathogens have evolved proteins to counteract NRC activities and suppress plant defense responses, leading to the continuous evolution of NRC networks.
Article
Biochemistry & Molecular Biology
Xia Yan, Bozeng Tang, Lauren S. Ryder, Dan MacLean, Vincent M. Were, Alice Bisola Eseola, Neftaly Cruz-Mireles, Weibin Ma, Andrew J. Foster, Miriam Oses-Ruiz, Nicholas J. Talbot
Summary: The rice blast fungus is a major threat to global rice production. This study provides a detailed understanding of the biology behind the invasion of plant tissues during the disease. Through transcriptional profiling, the study identifies specific gene expression changes and a repertoire of effectors critical for successful infection.
Meeting Abstract
Sport Sciences
Rafael F. Escamilla, Kyle Yamashiro, Robert J. Asuncion, Dan MacLean, Michael McKeough
MEDICINE & SCIENCE IN SPORTS & EXERCISE
(2022)
Meeting Abstract
Biochemistry & Molecular Biology
Z. Ma, R. K. Shrestha, T. Song, T. Kroj, E. Thynne, A. Hinchliffe, H. J. Schoonbeek, C. J. Ridout, S. Fairhead, P. F. Sarris, M. J. Moscou, K. V. Krasileva, D. MacLean, W. Harwood, J. D. Jones
MOLECULAR PLANT-MICROBE INTERACTIONS
(2019)
