Article
Biochemistry & Molecular Biology
Sarah Barakat, Melike Berksoz, Pegah Zahedimaram, Sofia Piepoli, Batu Erman
Summary: Camelidae derived single-domain antibodies, known as nanobodies, are small antibody fragments with full antigen-binding capacity. They have gained popularity due to their small size, high specificity, strong affinity, excellent stability, and modularity. Nanobodies are now widely used as molecular probes in the field of molecular imaging, allowing the detection and tracking of various targets. This review provides an overview of their applications and discusses current techniques for their generation and delivery.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Review
Biochemistry & Molecular Biology
Hai Shin Pung, Gee Jun Tye, Chiuan Herng Leow, Woei Kean Ng, Ngit Shin Lai
Summary: Cancer is a major cause of death globally, with nearly 10 million deaths in 2020. The lack of effective screening methods and early detection contributes to the high mortality rate, preventing early intervention. Non-invasive and deep-tissue imaging is beneficial for cancer diagnosis, and targeting ligands can enhance sensitivity and specificity. Phage display technology identifies ligands with binding specificity for target receptors. Tumor-targeting peptides show promise in molecular imaging, and nanotechnology enables the design of more potent imaging probes for cancer diagnosis and targeting therapy.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Nanoscience & Nanotechnology
Ji Hong Kim, Hye-Seon Jeong, Jaehyeon Hwang, Dae-Hyuk Kweon, Chang-Hyung Choi, Jong Pil Park
Summary: A sensor based on affinity peptide-immobilized hydrogel microspheres has been developed for the selective detection of influenza A virus. The sensor shows rapid and accurate detection capability, which is crucial for the effective control of pandemic flu outbreaks.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Biochemistry & Molecular Biology
Hawk Leong Yuen, Szn Yi Chan, Yi En Ding, Suxing Lim, Gim Cheong Tan, Chiew Ling Kho
Summary: This study aimed to develop a potent antibacterial peptide (ABP) with minimal toxicity through phage display selection and computer-assisted modification. A peptide called PAM-5 with enhanced antibacterial features was obtained by modifying the peptide selected for its high affinity to Pseudomonas aeruginosa cell surface using an online peptide database. PAM-5 showed activity against various Gram-negative and selected Gram-positive bacteria, and was stable in human plasma without toxic effects on human cells and erythrocytes.
Review
Microbiology
Weronika Jaroszewicz, Joanna Morcinek-Orlowska, Karolina Pierzynowska, Lidia Gaffke, Grzegorz Wegrzyn
Summary: Phage display technology is a widely applicable method in biotechnology, recognized by the Nobel Prize. This article provides an overview of the technology, including comparisons of different display systems, their advantages and disadvantages, and examples of applications in various fields. It also discusses other peptide display technologies and highlights the ongoing development and improvements in these methods, offering new solutions in the future.
FEMS MICROBIOLOGY REVIEWS
(2022)
Article
Chemistry, Multidisciplinary
Xiang Ji, Dan Liu, Feng Wu, Yu Cen, Lan Ma
Summary: This study aimed to prepare foam cell-specific polypeptides using a phage display platform for rapid detection of early atherosclerosis lesions and research on foam cell formation mechanisms and the development of blocking drugs.
APPLIED SCIENCES-BASEL
(2022)
Article
Microbiology
Chenglong Ma, Chunyang Jiang, Dongping Zhao, Shuhao Li, Ronggui Li, Lei Li
Summary: Directed evolution is a widely-used engineering strategy for improving protein stability or biochemical functions through repeated rounds of mutation and selection. Using ELISA to check the displayed protein against the target and enhancing the signal through various approaches can improve the sensitivity of ELISA experiments when dealing with low-affinity ligand-protein interactions.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Junxia Wang, Liang Li, Yingkun Zhang, Kaifeng Zhao, Xiaofeng Chen, Haicong Shen, Yuanqiang Chen, Jia Song, Yuqiang Ma, Chaoyong Yang, Hongming Ding, Zhi Zhu
Summary: Efficient molecular selection is crucial for various biomedical applications. By utilizing deterministic lateral-displacement microfluidics, the equilibrium of dissociation can be greatly shifted, leading to improved selection efficiency and better discrimination of ligand affinity.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Ruiqi Huang, Gabrielle Warner Jenkins, Yunjeong Kim, Robyn L. Stanfield, Amrinder Singh, Maria Martinez-Yamout, Gerard J. Kroon, Jonathan L. Torres, Abigail M. Jackson, Abigail Kelley, Namir Shaabani, Baisen Zeng, Michael Bacica, Wen Chen, Christopher Warner, Jasmina Radoicic, Joongho Joh, Krishani Dinali Perera, Huldah Sang, Tae Kim, Jianxiu Yao, Fangzhu Zhao, Devin Sokd, Dennis R. Burton, Jeff Allen, William Harriman, Waithaka Mwangi, Donghoon Chung, John R. Teijaro, Andrew B. Ward, H. Jane Dyson, Peter E. Wright, Ian A. Wilson, Kyeong-Ok Chang, Duncan Mcgregor, Vaughn V. Smider
Summary: Antibodies derived from cows have the potential to be a new class of therapeutics, capable of neutralizing various SARS-CoV-2 variants and providing protection against disease.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Chemistry, Multidisciplinary
Laura M. De Plano, Domenico Franco, Martina Bonsignore, Enza Fazio, Sebastiano Trusso, Alessandro Allegra, Caterina Musolino, Riccardo Cavaliere, Guido Ferlazzo, Fortunato Neri, Salvatore P. P. Guglielmino
Summary: The study evaluated the use of phage clones as probes for identifying multiple plasma cell immunophenotypes in MM patients. Selected phage clones were able to discriminate different MM immunophenotypes related to CD45, CD38, CD56, and CD138, suggesting the potential of using phage-fluorescence probes for rapid detection and characterization of patient disease status. A multi-phage imaging panel design could represent a highly sensitive approach for examining the presence/absence of CDs associated with disease.
APPLIED SCIENCES-BASEL
(2021)
Review
Chemistry, Analytical
Ping Xu, Subhadeep Ghosh, Anam Rana Gul, Jigna R. Bhamore, Jong Pil Park, Tae Jung Park
Summary: Biosensors have seen rapid growth in the field of biological detection and biomedical diagnosis, with displayed peptides from phage display libraries being recognized as a promising alternative to antibodies. The advantages of displayed peptides include high affinity, low cost, ease of design and modification, mass production, and increased resistance to complex environments.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Engineering, Environmental
Tianyang You, Yuan Ding, He Chen, Guangyue Song, Lianrun Huang, Minghua Wang, Xiude Hua
Summary: Competitive and noncompetitive immunoassays for clothianidin based on phage-displayed peptides were developed to improve sensitivity and specificity. The noncompetitive method showed the best specificity and sensitivity for clothianidin, with no cross-reactivity with analogs. The results demonstrated good recoveries and consistency with high-performance liquid chromatography in the analysis of blind samples.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Multidisciplinary Sciences
Makoto Nakakido, Naoki Tanaka, Ayako Shimojo, Nobuhiro Miyamae, Kouhei Tsumoto
Summary: Respiratory infectious diseases pose a serious global threat, and novel antiviral materials are in high demand. In this study, a new platform was constructed to evaluate antiviral activity of surface coating materials using the M13 bacteriophage and phagemid system. The experimental system developed in this study provides rapid and effective screening, which can be applied to the development of novel antiviral materials.
Article
Biotechnology & Applied Microbiology
Claire Milsted, Bo Dai, Nelson Garcia, Lu Yin, Yan He, Shahryar Kianian, Wojciech Pawlowski, Changbin Chen
Summary: This study used phage display to investigate the interactions of maize RAD51A1 with other proteins. The results revealed several peptide sequences that bind to ZmRAD51A1, supporting a potential role for ZmRAD51A1 in transcriptional regulation and plant defense.
Article
Biochemistry & Molecular Biology
K. M. Koss, T. Son, C. Li, Y. Hao, J. Cao, M. A. Churchward, Z. J. Zhang, J. A. Wertheim, R. Derda, K. G. Todd
Summary: Microglia play critical roles in the development and healthy function of the brain and spinal cord, but they are also involved in the pathology of various neuropsychiatric disorders. In this study, researchers explored the functional phenotypes of microglia and mixed microglia/astrocytes using in vitro models. They hypothesized that the biomolecular profile of these cells undergoes phenotypical changes, and they searched for novel peptide binders using a custom phage library. By studying the secretion of cytokines and nitric oxide and analyzing the expression of inducible nitric oxide synthase, they identified 58 unique peptides with potential functions related to glial support, inflammation activation, and regulation in neurodegenerative and neuropsychiatric disorders.
JOURNAL OF NEUROCHEMISTRY
(2023)
Article
Radiology, Nuclear Medicine & Medical Imaging
Yannick Rossez, Carmen Burtea, Sophie Laurent, Pierre Gosset, Renaud Leonard, Walter Gonzalez, Sebastien Ballet, Isabelle Raynal, Olivier Rousseaux, Timothee Dugue, Luce Vander Elst, Jean-Claude Michalski, Robert N. Muller, Catherine Robbe-Masselot
CONTRAST MEDIA & MOLECULAR IMAGING
(2016)
Article
Radiology, Nuclear Medicine & Medical Imaging
Mario Dentamaro, Francois Lux, Luce Vander Elst, Nicolas Dauguet, Sylvie Montante, Albert Moussaron, Carmen Burtea, Robert N. Muller, Olivier Tillement, Sophie Laurent
CONTRAST MEDIA & MOLECULAR IMAGING
(2016)
Article
Neurosciences
Severine Andre, Emilie Ansciaux, Elamine Saidi, Lionel Larbanoix, Dimitri Stanicki, Denis Nonclercq, Luce Vander Elst, Sophie Laurent, Robert N. Muller, Carmen Burtea
JOURNAL OF ALZHEIMERS DISEASE
(2017)
Article
Oncology
Deborah Fanfone, Nadege Despretz, Dimitri Stanicki, Jenifer Rubio-Magnieto, Mathieu Fossepre, Mathieu Surin, Sandrine Rorive, Isabelle Salmon, Luce Vander Elst, Sophie Laurent, Robert N. Muller, Sven Saussez, Carmen Burtea
Article
Materials Science, Multidisciplinary
Mihaela Balas, Carmen Steluta Ciobanu, Carmen Burtea, Miruna Silvia Stan, Eugenia Bezirtzoglou, Daniela Predoi, Anca Dinischiotu
Article
Biochemistry & Molecular Biology
Stephane Demine, Alexander Balhuizen, Vinciane Debaille, Lieke Joosten, Maite Fereau, Satya Narayana Murthy Chilla, Isabelle Millard, Raphael Scharfmann, Dominique Egrise, Serge Goldman, Piero Marchetti, Martin Gotthardt, Sophie Laurent, Carmen Burtea, Decio L. Eizirik
Article
Pharmacology & Pharmacy
Deborah Crombez, Sebastien Delcambre, Denis Nonclercq, Luce Vander Elst, Sophie Laurent, Miriam Cnop, Robert N. Muller, Carmen Burtea
JOURNAL OF DRUG TARGETING
(2020)
Article
Neurosciences
Jerome Canei, Carmen Burtea, Denis Nonclercq
Article
Chemistry, Physical
Ioana Mihaela Popescu Din, Mihaela Balas, Anca Hermenean, Luce Vander Elst, Sophie Laurent, Carmen Burtea, Ludmila Otilia Cinteza, Anca Dinischiotu
Article
Biology
Severine Andre, Lionel Larbanoix, Sebastien Verteneuil, Dimitri Stanicki, Denis Nonclercq, Luce Vander Elst, Sophie Laurent, Robert N. Muller, Carmen Burtea
Editorial Material
Radiology, Nuclear Medicine & Medical Imaging
Sebastien Boutry, Sophie Laurent, Carmen Burtea
JOURNAL OF MAGNETIC RESONANCE IMAGING
(2022)
Review
Pharmacology & Pharmacy
Segbede E. R. Tcheoubi, Casimir D. Akpovi, Frederique Coppee, Anne-Emilie Decleves, Sophie Laurent, Clement Agbangla, Carmen Burtea
Summary: This review summarises the current knowledge on the link between PCSK9 and T2DM. Significant correlations were found between PCSK9 and insulin, insulin resistance (HOMA), and glycated haemoglobin. Loss-of-function variants in PCSK9 were found to increase the risk of T2DM by altering insulin secretion. Low PCSK9 levels in the pancreas were found to regulate LDL receptor expression, leading to intracellular cholesterol accumulation and impaired insulin secretion. However, the association between PCSK9 loss-of-function variants and T2DM is inconsistent. These associations may vary depending on the genetic background of the studied populations and the specific effects of the variants on PCSK9 protein. These factors should be considered when assessing the long-term impact of PCSK9i on glucose homeostasis.
JOURNAL OF DRUG TARGETING
(2022)
Article
Biochemistry & Molecular Biology
Mihaela Balas, Daniela Predoi, Carmen Burtea, Anca Dinischiotu
Summary: Iron oxide nanoparticles coated with dextran were characterized and their effects on pancreatic cancer cells were investigated. The nanoparticles showed significant negative contrast in MRI and dose-dependent cellular iron uptake and toxicity levels. At low doses, these nanoparticles could serve as safe platforms for drug delivery, as well as antitumoral and imaging agents in pancreatic cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biology
Deborah Fanfone, Dimitri Stanicki, Denis Nonclercq, Marc Port, Luce Vander Elst, Sophie Laurent, Robert N. Muller, Sven Saussez, Carmen Burtea
Article
Rheumatology
Carmen Burtea, Sophie Laurent, Tuba Sanli, Deborah Fanfone, Aude Devalckeneer, Sebastien Sauvage, Marie-Claire Beckers, Sandrine Rorive, Isabelle Salmon, Luce Vander Elst, Bernard R. Lauwerys, Robert N. Muller
ARTHRITIS RESEARCH & THERAPY
(2016)