Article
Chemistry, Applied
Tessa B. Moyer, Wyatt J. Schug, Leslie M. Hicks
Summary: This study utilizes in silico predictions, proteomics, and simulated gastrointestinal digestions to identify digestion resistant cysteine-rich peptides (CRPs) within Amaranthus hypochondriacus seeds. Thirteen in silico predicted CRPs were detected in a seed extract, providing evidence for the translation of five CRP families. Mature forms of six CRPs were characterized via top-down proteomics, revealing multiple post translational modifications. All six peptides demonstrated resistance to simulated gastrointestinal digestion, suggesting that A. hypochondriacus CRPs may exhibit bioactivity after consumption and should be prioritized for further characterization.
Article
Cardiac & Cardiovascular Systems
Tlili Barhoumi, Fatmah A. Mansour, Maroua Jalouli, Hassan S. Alamri, Rizwan Ali, Abdel Halim Harrath, Maha Aljumaa, Mohamed Boudjelal
Summary: Angiotensin II (Ang II) is a key component of the renin-angiotensin-aldosterone system and is associated with cardiopathology. High levels of Ang II have been linked to inflammatory conditions such as coronary heart disease, obesity, and type 2 diabetes. This study evaluated the cellular effects of Ang II on THP-1-derived macrophages, showing that it stimulates differentiation markers and proinflammatory markers while decreasing an M2 marker. Ang II also induces calcium overload, increases reactive oxygen species, and arrests cells in the G1 phase, primarily through the angiotensin II type 1 receptor (AT1R).
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Kiran Shabir, Seley Gharanei, Sophie Orton, Vanlata Patel, Parbata Chauhan, Emmanouil Karteris, Harpal S. Randeva, James E. Brown, Ioannis Kyrou
Summary: Adipose tissue functions as an endocrine organ by secreting various adipokines that regulate metabolism and other physiological processes. The altered secretion of adipokines has been associated with obesity and cardio-metabolic diseases. Asprosin, a recently discovered adipokine, is elevated in cardio-metabolic diseases and has pro-inflammatory effects, at least in part through the TLR4 signaling pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Pornwipa Phuangbubpha, Sanya Thara, Patsawee Sriboonaied, Puretat Saetan, Wanwiwa Tumnoi, Adisri Charoenpanich
Summary: This study presents a long-term (21-day) triple co-culture model of THP-1 macrophages, Caco-2 intestinal epithelial cells, and HT-29-methotrexate goblet cells. The macrophages were stably differentiated and could be cultured for up to 21 days, confirming their potential for long-term immune response modeling. The model successfully demonstrated cytokine secretions during inflammation and maintained intestinal membrane integrity.
Article
Immunology
Monica Dominguez, Verena Truemper, Ana Carolina Mota, Bernhard Bruene, Dmitry Namgaladze
Summary: ATP-citrate lyase (ACLY) is a key enzyme involved in metabolic and epigenetic gene regulation. This study investigated the impact of ACLY phosphorylation on its function in human myeloid cells and found that phosphorylation at serine 455 does not affect the enzymatic activity of ACLY. Additionally, loss of ACLY activity may trigger long-term adaptive mechanisms that increase inflammatory responses.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Cell Biology
Jun Zhou, Yuhui Que, Lihua Pan, Xu Li, Chao Zhu, Lai Jin, Shengnan Li
Summary: SVIL, a protein belonging to the villin/gelsolin family, is involved in regulating macrophage movement and LPS-induced inflammation. The study demonstrated that LPS induced SVIL expression by activating TLR4/NF-kappa B and ERK1/2 MAPK pathways, and SVIL participated in the inflammatory response induced by LPS.
Article
Biochemistry & Molecular Biology
Diletta Ami, Ana Rita Franco, Valentina Artusa, Paolo Mereghetti, Francesco Peri, Antonino Natalello
Summary: The study utilizing FTIR microspectroscopy to analyze the effects of LPS on macrophage-like cells demonstrated the potential for this method to identify spectroscopic markers of inflammation activation. It could become a tool to assess global biochemical changes related to inflammatory and anti-inflammatory stimuli.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Applied
Fabiola Sanchez-Lopez, Victor J. Robles-Olvera, Madeleine Hidalgo-Morales, Apollinaire Tsopmo
Summary: This study aimed to investigate the in vitro antihypertensive activities of lactobacillus-prepared amaranth protein hydrolysates, determine the contribution of zinc, and identify peptides. The findings showed that lactic acid bacteria proteases released peptides from amaranth proteins with ACE-inhibitory properties, some of which contained known ACE-inhibitor motifs. Size separation of the hydrolysates enhanced ACE inhibition, supporting a mixed mechanism of inhibition.
Article
Cell Biology
Gina M. Gallucci, Bader Alsuwayt, Adam M. Auclair, James L. Boyer, David N. Assis, Nisanne S. Ghonem
Summary: Fenofibrate has anti-inflammatory effects in chronic liver diseases by inhibiting NF-kappa B signaling and reducing the secretion of pro-inflammatory cytokines.
Article
Environmental Sciences
Ziyi Wei, Weiguo Wang, Wen Fu, Ping Zhang, Hao Feng, Wenping Xu, Liming Tao, Zhong Li, Yang Zhang, Xusheng Shao
Summary: In this study, the immune toxic effects of emamectin benzoate (EMB) on THP-1 macrophages were evaluated. EMB was found to inhibit phagocytic activity and respiratory burst capacity of macrophages without inducing cellular toxicity. Moreover, EMB disrupted the cytokines balance and could activate the NF-kappa B pathway in THP-1 macrophages.
ENVIRONMENTAL TOXICOLOGY
(2023)
Article
Oncology
Katharina M. Scheurlen, Dylan L. Snook, Andrew B. Littlefield, Joan B. George, Mary A. Parks, Robert J. Beal, Anne MacLeod, Daniel W. Riggs, Jeremy T. Gaskins, Julia Chariker, Eric C. Rouchka, Susan Galandiuk
Summary: Macrophages play a crucial role in cancer development, particularly in tumor progression and poor patient survival. However, there is currently a lack of standardized and validated models for tumor-associated macrophages (TAMs). This study characterized and validated a TAM-like M2 subtype macrophage model derived from the human THP-1 cell line, providing a standardized model for investigating anti-inflammatory mechanisms in various settings, with a focus on colon cancer development.
Article
Immunology
Tlili Barhoumi, Bandar Alghanem, Hayat Shaibah, Fatmah A. Mansour, Hassan S. Alamri, Maaged A. Akiel, Fayhan Alroqi, Mohammad Boudjelal
Summary: The study found that the S protein of SARS-CoV-2 can induce apoptosis, oxidative stress, and inflammatory responses in macrophages and endothelial cells, which can be mitigated by the ACE inhibitor perindopril. Additionally, the S protein can affect the mitogenic responses and proteomic regulation of PBMCs. These findings suggest that the S protein may activate blood and vascular components circulating in the body, with potential involvement of the local renin-angiotensin system in this process.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Ana Marina Barroso de Figueiredo, Dayane Moraes, Alexandre Melo Bailao, Olivia Basso Rocha, Lana Ohara Souza Silva, Fatima Ribeiro-Dias, Celia Maria de Almeida Soares
Summary: Proteomic analysis revealed the impact of P. brasiliensis yeast cells on the macrophage proteome, indicating metabolic changes, altered energy production pathways, epigenetic modifications, gene transcription, and immune system activity. These findings contribute to understanding the host response to P. brasiliensis and identifying potential therapeutic targets for PCM.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Chemistry, Applied
Alexis Ayala-Nino, Araceli Castaneda-Ovando, Judith Jaimez-Ordaz, Gabriela Mariana Rodriguez-Serrano, Jose Antonio Sanchez-Franco, Luis Guillermo Gonzalez-Olivares
Summary: This study demonstrated that in vitro enzymatic digestion allows the release of multifunctional bioactive peptide fractions from amaranth proteins.
NATURAL PRODUCT RESEARCH
(2022)
Article
Pharmacology & Pharmacy
Shramana Koner, Ida Florance, Amitava Mukherjee, Natarajan Chandrasekaran
Summary: This study investigates the toxic effects of polystyrene nanoparticles (PSNPs) on human macrophages. The results show that exposure to PSNPs leads to cytotoxicity, oxidative stress, decreased cell proliferation, decreased mitochondrial membrane potential, and DNA damage in human macrophages.
Article
Immunology
Xiaoqing Xu, Yu Qiao, Qing Peng, Bo Shi, Vermont P. Dia
Summary: The EPS from Lactobacillus casei NA-2 demonstrated antioxidant activity and potential immunomodulatory effects in vitro, indicating its potential as a natural substance to combat oxidative damage.
IMMUNOLOGICAL INVESTIGATIONS
(2022)
Article
Food Science & Technology
Xiaoqing Xu, Yu Qiao, Bo Shi, Vermont P. Dia
Summary: In this study, protein isolates from pigeon pea, lentil, and chickpea were hydrolyzed by Alcalase and bromelain. The effects of the two enzymes on the hydrolysis of the proteins were compared, showing differences in scavenging capacity of NO radicals and antioxidant activities.
FOOD STRUCTURE-NETHERLANDS
(2021)
Article
Food Science & Technology
Shan Hong, Vermont P. Dia, Seung Joon Baek, Qixin Zhong
Summary: This study successfully encapsulated apigenin with whey protein isolate through nanoencapsulation, maintaining its anticancer activity and enhancing cellular uptake, proapoptotic effects, and bioavailability, which may be significant for incorporating lipophilic phytochemicals in functional beverages for disease prevention.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Agriculture, Multidisciplinary
F. M. A. Leyva-Gutierrez, M. Fomich, C. Metzcar, J. Saad, V. P. Dia, J. A. Cammack, J. K. Tomberlin, T. Wang
Summary: The lipid, protein, chitin, and mineral composition of black soldier fly larvae and adults reared on spent brewer's grain and Gainesville diet were analyzed. The study found that the lipid profile of larvae was dominated by triacylglycerol-bound fatty acids, while adults had a similar fatty acid profile with free fatty acids as the dominant species. The protein composition of both larvae and adults was dominated by glutelins. This information will contribute to the utilization of black soldier fly larvae and adults in food, food ingredients, and feed.
JOURNAL OF INSECTS AS FOOD AND FEED
(2022)
Article
Food Science & Technology
Andrea Nieto-Veloza, Zhihong Wang, Qixin Zhong, Doris D'Souza, Hari B. Krishnan, Vermont P. Dia
Summary: This study assessed the in vivo anti-inflammatory properties of Lunasin protease inhibitor concentrate (LPIC). In vitro tests showed that LPIC induced the production of nitric oxide and maintained cytokine levels. In vivo experiments demonstrated that LPIC significantly attenuated tissue damage in ulcerative colitis.
FOOD SCIENCE AND HUMAN WELLNESS
(2022)
Article
Agriculture, Multidisciplinary
Anyi Wang, Surangna Jain, Vermont Dia, Scott C. Lenaghan, Qixin Zhong
Summary: Nanoencapsulating curcumin in shellac micelles with a diameter smaller than 50 nm showed advantages in cancer prevention. The nanocapsules exhibited high loading capacity and encapsulation efficiency, maintained their structure during storage, and showed enhanced bioavailability and antiproliferation activity against colon cancer cells.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Irene Tome-Sanchez, Cristina Martinez-Villaluenga, Ana Belen Martin-Diana, Daniel Rico, Ivan Jimenez-Pulido, Juana Frias, Vermont P. Dia
Summary: This study evaluated the immunostimulatory effect of a wheat bran hydrolysate and a mousse enriched with the hydrolysate on macrophages, and analyzed the antiproliferative activity of the macrophage supernatant on colorectal cancer cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Bruna P. Tischer, Philipus Pangloli, Andrea Nieto-Veloza, Matthew Reeder, Vermont Dia
Summary: The purpose of this study was to extract and identify the phenolic compounds in two native fruits from southern region of Brazil and evaluate their antioxidant and anti-inflammatory properties. Tannins were the most prevalent phenolic compounds in the fruits, with higher concentrations observed in the methanol extracts. Gallic acid, catechin, vanillic acid, and ellagic acid were identified as the most abundant phenolic compounds. The extracts exhibited significant antioxidant and anti-inflammatory activity, inhibiting the scavenging of DPPH and NO radicals, as well as reducing the release of pro-inflammatory molecules NO and IL-6.
Article
Food Science & Technology
Joshua S. Saad, Vermont P. Dia
Summary: In this study, hempseed protein isolate (HPI-12) was produced from industrial hempseed hearts using a pH 12 method. Bromelain was used to hydrolyze HPI-12 (BHPI-12) and assess its functionality and bioactivity. HPI-12 showed antioxidant and anti-inflammatory properties by reducing DPPH radicals and pro-inflammatory cytokines. Hydrolysis improved protein solubility and enhanced antioxidant and anti-inflammatory activity. This study reports the potential of HPI-12 and BHPI-12 as functional food ingredients with antioxidant and anti-inflammatory benefits.
ACS FOOD SCIENCE & TECHNOLOGY
(2023)
Article
Chemistry, Applied
Yuan Yuan, Madison Fomich, Vermont P. Dia, Tong Wang
Summary: The aim of this research was to enhance the ice recrystallization inhibition (IRI) activity of zein and gelatin hydrolysates (ZH and GH) by succinylation modification. Modified hydrolysates (SA modified ZH and OSA modified GH) decreased the average Feret's diameter of ice crystal from 50.2 μm to 28.8 μm and 29.5 μm, respectively. Succinylated samples also exhibited altered surface hydrophobicity, potentially contributing to their enhanced IRI activity. This study demonstrates that succinylation of food-derived protein hydrolysates can improve their IRI activity.
Article
Agriculture, Multidisciplinary
Madison Fomich, Vermont P. Dia, Uvinduni I. Premadasa, Benjamin Doughty, Hari B. Krishnan, Tong Wang
Summary: The study investigated the ice recrystallization inhibition (IRI) activity of soy protein isolate (SPI) hydrolysates and determined that the F1 fraction (4-14 kDa) had the highest IRI activity. Vibrationalsum frequency generation (VSFG) analysis indicated that the molecular interactions at the water/air interface were linked to IRI activity.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Andrea Nieto-Veloza, Shan Hong, Matthew Reeder, Mee-Ja Sula, Doris H. D'Souza, Qixin Zhong, Vermont P. Dia
Summary: This study investigated the protective effect of the soy peptide lunasin in an in vivo model of susceptibility to inflammatory bowel disease (IBD) and identified its potential mechanism of action in vitro. The results showed that lunasin could reduce susceptibility to inflammation and levels of proinflammatory cytokines. Lunasin exhibited anti-inflammatory properties and decreased susceptibility to IBD in genetically susceptible mice.
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Xiaoqing Xu, Yu Qiao, Qing Peng, Vermont Punongba Dia, Bo Shi
Summary: This study evaluated the probiotic properties of a ropy Latilactobacillus isolated from Chinese northeast sauerkraut and compared the antioxidant and immunoregulatory activities of its heat-killed cells and live strains. The results showed that heat-killed L. plantarum NA exhibited antioxidant and immune-stimulating activities similar to live cells, possibly due to the undamaged overall structure after heating.
Article
Chemistry, Applied
Shan Hong, Yang Lin, Vermont P. Dia
Summary: There is growing interest in using industrial hempseed as a potential food ingredient due to its health-promoting properties. This study aimed to produce hempseed protein hydrolysates with antioxidant and anti-inflammatory properties. The results showed that the hydrolysates had the potential to inhibit inflammation and act as natural antioxidants.
FOOD HYDROCOLLOIDS FOR HEALTH
(2022)
Article
Food Science & Technology
Svjetlana Matic, Vermont P. Dia
Summary: Exosomes affect macrophages under hypoxia by increasing cell viability, reducing ROS production, and influencing the cell cycle.
CURRENT RESEARCH IN FOOD SCIENCE
(2022)