Cyanidin-3-O-ß-glucoside upregulates hepatic cholesterol 7a-hydroxylase expression and reduces hypercholesterolemia in mice

Scope: Although previous studies have shown that consumption of anthocyanin extract from plant foods reduces hypercholesterolemia and the severity of atherosclerosis in different animal models, the mechanisms of these actions remained unclear. This study investigated whether pure anthocyanin inhibit atherosclerosis development and reduce hypercholesterolemia in the apolipoprotein E (ApoE)-deficient mice through enhancement of fecal bile acid excretion, a critical pathway for eliminating circulation cholesterol from the body. Methods and results: Five-week-old male ApoE-deficient mice were fed the AIN-93G diet supplemented with or without cyanidin-3-O-beta-glucoside (0.06% w/w) for 12 weeks. Results showed that cyanidin-3-O-beta-glucoside consumption inhibited the formation of aortic sinus plaque and reduced hypercholesterolemia, along with promoted fecal bile acid excretion and upregulated hepatic cholesterol 7a-hydroxylase expression (CYP7A1). In mouse primary hepatocytes, cyanidin-3-O-beta-glucoside treatment increased bile acid synthesis and CYP7A1 expression in a liver X receptor alpha (LXR alpha)-)-dependent manner. Scintillation proximity and time-resolved fluorescence resonance energy transfer assays revealed that cyanidin-3-O-beta-glucoside functions as an agonist of LXR alpha. Conclusion: Our results indicate that the hypocholesterolemic activity of cyanidin-3-O-beta-glucoside was, at least in part, mediated by activating the potential LXR alpha-CYP7A1-bile acid excretion pathway, thus contributing to the antiatherogenic effect of cyanidin-3-O-beta-glucoside. Importantly, cyanidin-3-O-beta-glucoside could activate LXR alpha in an agonist-dependent manner.