Article
Biotechnology & Applied Microbiology
Marianne Grognot, Anisha Mittal, Mattia Mah'moud, Katja M. Taute
Summary: Cholera disease caused by Vibrio cholerae results in severe diarrhea and vomiting, with approximately 100,000 deaths per year worldwide. The bacterium's ability to swim aids in infection by potentially crossing the protective mucus barrier in the host environment. The bacterium exhibits asymmetric run-reverse-flick motility, with shorter backward runs and fluctuating swimming speeds, in all environments studied.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Microbiology
Dianshu Zhao, Afsar Ali, Cameron Zuck, Laurice Uy, J. Glenn Morris Jr, Adam Chun-Nin Wong
Summary: Vibrio cholerae, the causative agent of cholera, forms symbiotic associations with aquatic arthropods to facilitate its environmental persistence and dissemination. The dynamics of interactions between V. cholerae and aquatic arthropods remain largely unknown. This study developed a new experimental host system using chironomid larvae to investigate the effects of bacterial cell density and strain on V. cholerae-chironomid interactions. The findings highlight the importance of cell density in determining V. cholerae invasion success in chironomid larvae and provide insights into V. cholerae-arthropod interactions using a novel approach.
MICROBIOLOGY SPECTRUM
(2023)
Article
Multidisciplinary Sciences
Jiandong Chen, Hyuntae Byun, Rui Liu, I-Ji Jung, Qinqin Pu, Clara Y. Zhu, Ethan Tanchoco, Salma Alavi, Patrick H. Degnan, Amy T. Ma, Manuela Roggiani, Joris Beld, Mark Goulian, Ansel Hsiao, Jun Zhu
Summary: The genotoxin colibactin produced by commensal Escherichia coli can not only induce DNA damage in host intestinal cells, but also target a variety of enteric pathogens and commensals via a contact-dependent mechanism. Furthermore, colibactin can shape the microbiome by species-specific targeting of Bacteroides fragilis, a common gut-associated microbe.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Food Science & Technology
Shruti Chatterjee, Ishan Raval, Krishna Raval, Rima Tapader, Gopal Bhojani, Amit Pal, Sandhya Mishra
Summary: The study demonstrated that an extract from edible microalgae can inhibit the production of virulence factors in pathogenic Vibrio cholerae strains, reducing cholera toxin production and cell-cytotoxicity. In vivo study on Artemia salina also showed slightly diminished colonization of V. cholerae in the presence of the extract. GC-MS analysis identified various fatty acids in the extract, suggesting its potential as a natural source for functional food and pharmaceutical products against cholera.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Agriculture, Multidisciplinary
Dingkang Wang, Hong Chen, Huan Yang, Shangjie Yao, Chongde Wu
Summary: Exogenous fatty acids have significant effects on the salt tolerance of food yeast, with four specific fatty acids enhancing cell salt tolerance and promoting cell biofilm formation and chemical composition regulation.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Benjamin X. Wang, Julie Takagi, Abigail McShane, Jin Hwan Park, Kazuhiro Aoki, Catherine Griffin, Jennifer Teschler, Giordan Kitts, Giulietta Minzer, Michael Tiemeyer, Rachel Hevey, Fitnat Yildiz, Katharina Ribbeck
Summary: We found that mucin polymers are potent regulators that block toxigenic conversion and suppress the expression of virulence factors in V. cholerae, mediated by CTX phi. This discovery highlights the novel role of mucins and their associated O-glycan structures in modulating the evolution and pathogenicity of V. cholerae through interfering with the TcpP/ToxR/ToxT virulence pathway.
Article
Environmental Sciences
Sadok Khouadja, Ana Roque, Mar Gonzalez, Dolors Furones
Summary: In this study, we investigated the presence of Vibrio cholerae virulence genes in Vibrio alginolyticus isolates and found that some of the V. alginolyticus strains might have derived their virulence genes from V. cholerae, making them a possible reservoir of V. cholerae virulence genes.
JOURNAL OF WATER AND HEALTH
(2022)
Article
Biology
Kelsey Barrasso, Denise Chac, Meti D. Debela, Catherine Geigel, Anjali Steenhaut, Abigail Rivera Seda, Chelsea N. Dunmire, Jason B. Harris, Regina C. Larocque, Firas S. Midani, Firdausi Qadri, Jing Yan, Ana A. Weil, Wai-Leung Ng, Melanie Blokesch
Summary: Recent studies suggest that the presence of Paracoccus aminovorans, a commensal bacterium, in the human intestine may enhance the colonization of Vibrio cholerae. The interaction between the two species was shown to form a dual-species biofilm structure with novel features, and this interaction was found to be dependent on the Vibrio exopolysaccharide and other major components of mature V. cholerae biofilm. These findings demonstrate that multispecies biofilm formation could be a mechanism used by gut microbes to increase the virulence of pathogens and potentially impact the outcomes of enteric infections.
Article
Immunology
Dustin A. Farr, Dhrubajyoti Nag, Jeffrey H. Withey
Summary: The study reveals that zebrafish can serve as a natural host model for Vibrio cholerae, exhibiting similar diarrheal symptoms as humans. By assessing various immunological markers, it was found that infection with V. cholerae in zebrafish leads to increased gene expression of T-bet and GATA3, as well as elevated levels of mucosal antibodies.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Review
Microbiology
Roberto C. Molina-Quiroz, Cecilia A. Silva-Valenzuela
Summary: Bacteriophages are viruses that infect bacteria and have been used as a model system in microbial genetics and molecular biology. Understanding the arms race between bacteria and phages is important for the design of phage-based therapies for bacterial infections. Vibrio species and their phages provide a suitable model to study these interactions in aquatic environments.
CURRENT OPINION IN MICROBIOLOGY
(2023)
Article
Microbiology
Mei Luo, Guozhong Chen, Chunrong Yi, Baoshuai Xue, Xiaoman Yang, Yao Ma, Zixin Qin, Jin Yan, Xiaoyun Liu, Zhi Liu
Summary: During long-term colonization in adult mice, Vibrio cholerae (V. cholerae) increased its fitness by producing nonmotile mutants through deletion of the cyclin gene dps. Methionine metabolism pathway may be involved in the increased colonization in the adult mice.
Article
Microbiology
Aaron Nicholas Gillman, Anel Mahmutovic, Pia Abel Zur Wiesch, Soren Abel
Summary: The study reveals that during infection, the rates of bacterial replication, death, and migration have significant impacts on disease progression. Furthermore, there is substantial spatiotemporal heterogeneity in these dynamics in different host locations. Additionally, an increase in inoculum size leads to a larger niche available to V. cholerae in the host, suggesting cooperative effects during infection.
Article
Immunology
Jennifer Y. Cho, Rui Liu, Ansel Hsiao
Summary: The commensal microbes of the gut microbiota play an important role in defending against gastrointestinal pathogens. Different commensal communities contribute to individual differences in susceptibility to infections. This study reveals the different response mechanisms of susceptible and infection-resistant microbial communities to Vibrio cholerae infection, including differential gene expression related to oxidative stress and biofilm formation.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Prasanga Madhushani Kumarage, Liyana Arachchilage Dinithi Sandunika De Silva, Gang-Joon Heo
Summary: This article summarizes the antimicrobial resistance properties of Vibrio species isolated from aquatic environments to raise awareness about potential health risks related to Vibrio infections in aquaculture and public health.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Review
Environmental Sciences
Greta Kalvaitiene, Diana Vaiciute, Martynas Bucas, Greta Gyraite, Marija Katarzyte
Summary: Waterborne pathogenic bacteria, such as faecal indicator bacteria and potentially pathogenic Vibrio, pose a global concern for waterborne diseases. A systematic review found that brown algae and seagrasses had the highest quantities of faecal indicator bacteria, while red algae had the highest quantities of Vibrio bacteria. The research on macrophytes focused mainly on brown algae, with green algae being the least studied. Further research is needed to understand the role of potentially pathogenic Vibrio and faecal indicator bacteria with macrophytes and their microbiome in the coastal marine environment.
MARINE POLLUTION BULLETIN
(2023)
Article
Microbiology
Adrianna E. Eder, Saba A. Munir, Chelsea R. Hobby, Derek M. Anderson, Joshua L. Herndon, Andrew W. Siv, Steven J. K. Symes, David K. Giles
Article
Microbiology
Chelsea R. Hobby, Joshua L. Herndon, Colton A. Morrow, Rachel E. Peters, Steven J. K. Symes, David K. Giles
Article
Rehabilitation
Henry G. Spratt, David Levine, Julie Bage, David K. Giles, A. Grace Collier
PHYSIOTHERAPY THEORY AND PRACTICE
(2019)
Article
Microbiology
Lyssa Y. Baker, Chelsea R. Hobby, Andrew W. Siv, William C. Bible, Michael S. Glennon, Derek M. Anderson, Steven J. Symes, David K. Giles
Article
Immunology
Thomas W. Cullen, John P. O'Brien, David R. Hendrixson, David K. Giles, Rhonda I. Hobb, Stuart A. Thompson, Jennifer S. Brodbelt, M. Stephen Trent
INFECTION AND IMMUNITY
(2013)
Article
Immunology
Alexander C. Maue, Krystle L. Mohawk, David K. Giles, Frederic Poly, Cheryl P. Ewing, Yuening Jiao, Ginyoung Lee, Zuchao Ma, Mario A. Monteiro, Christina L. Hill, Jason S. Ferderber, Chad K. Porter, M. Stephen Trent, Patricia Guerry
INFECTION AND IMMUNITY
(2013)
Article
Biochemical Research Methods
James A. Madsen, Hua Xu, Michelle R. Robinson, Andrew P. Horton, Jared B. Shaw, David K. Giles, Tamer S. Kaoud, Kevin N. Dalby, M. Stephen Trent, Jennifer S. Brodbelt
MOLECULAR & CELLULAR PROTEOMICS
(2013)
Article
Multidisciplinary Sciences
Jessica V. Hankins, James A. Madsen, David K. Giles, Jennifer S. Brodbelt, M. Stephen Trent
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2012)
Article
Multidisciplinary Sciences
Brittany D. Needham, Sean M. Carroll, David K. Giles, George Georgiou, Marvin Whiteley, M. Stephen Trent
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2013)
Article
Microbiology
Aaron C. Pride, Carmen M. Herrera, Ziqiang Guan, David K. Giles, M. Stephen Trent
Article
Microbiology
Joshua L. Herndon, Rachel E. Peters, Rachel N. Hofer, Timothy B. Simmons, Steven J. Symes, David K. Giles
Article
Microbiology
David S. Smith, Carina Houck, Allycia Lee, Timothy B. Simmons, Olivia N. Chester, Ayanna Esdaile, Steven J. K. Symes, David K. Giles
Summary: The study found that Vibrio alginolyticus and Vibrio fischeri can respond to exogenous polyunsaturated fatty acids by modifying their membrane phospholipids and behaviors related to virulence. Different fatty acids showed varied effects on growth, membrane permeability, and swimming motility of the bacteria.
Article
Fisheries
Rachel N. Hofer, Allen Lin, Benjamin C. House, Christopher N. Purvis, Bradley J. Harris, Steven J. K. Symes, David K. Giles
Summary: This study explored the ability of Aeromonas salmonicida strains with and without the A-layer to incorporate polyunsaturated fatty acids (PUFAs) into their lipid profile and the phenotypic effects thereof. The results showed that, regardless of the presence of the A-layer, A. salmonicida strains were capable of incorporating all seven PUFAs studied. PUFA exposure led to changes in biofilm formation and membrane permeability, and some PUFAs offered protection or vulnerability against certain cyclic peptides. The strain-specific responses to exogenous PUFAs may indicate evolved adaptive strategies for survival, persistence, and virulence in bacteria that inhabit both environmental and fish host niches.
JOURNAL OF FISH DISEASES
(2023)
Article
Biotechnology & Applied Microbiology
Anna R. Moravec, Andrew W. Siv, Chelsea R. Hobby, Emily N. Lindsay, Layla V. Norbash, Daniel J. Shults, Steven J. K. Symes, David K. Giles
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2017)