Microscopy approaches to investigate protein dynamics and lipid organization

The organisation of the cell membrane: do proteins rule lipids?

(2014) Jérémie Rossy et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Laurdan Fluorescence Lifetime Discriminates Cholesterol Content from Changes in Fluidity in Living Cell Membranes

(2013) Ottavia Golfetto et al.   BIOPHYSICAL JOURNAL

Characterization of a New Series of Fluorescent Probes for Imaging Membrane Order

(2013) Joanna M. Kwiatek et al.   PLoS One

Partitioning, diffusion, and ligand binding of raft lipid analogs in model and cellular plasma membranes

(2012) Erdinc Sezgin et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Active Remodeling of Cortical Actin Regulates Spatiotemporal Organization of Cell Surface Molecules

(2012) Kripa Gowrishankar et al.   CELL

Advanced Fluorescence Microscopy Techniques—FRAP, FLIP, FLAP, FRET and FLIM

(2012) Hellen C. Ishikawa-Ankerhold et al.   MOLECULES

Transient GPI-anchored protein homodimers are units for raft organization and function

(2012) Kenichi G N Suzuki et al.   Nature Chemical Biology

Conformational states of the kinase Lck regulate clustering in early T cell signaling

(2012) Jérémie Rossy et al.   NATURE IMMUNOLOGY

Spectral phasor analysis allows rapid and reliable unmixing of fluorescence microscopy spectral images

(2012) Farzad Fereidouni et al.   OPTICS EXPRESS

Millisecond spatiotemporal dynamics of FRET biosensors by the pair correlation function and the phasor approach to FLIM

(2012) E. Hinde et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Sub-resolution lipid domains exist in the plasma membrane and regulate protein diffusion and distribution

(2012) Dylan M. Owen et al.   Nature Communications

Detecting Nanodomains in Living Cell Membrane by Fluorescence Correlation Spectroscopy

(2011) Hai-Tao He et al.   Annual Review of Physical Chemistry

STED Nanoscopy Reveals Molecular Details of Cholesterol- and Cytoskeleton-Modulated Lipid Interactions in Living Cells

(2011) V. Mueller et al.   BIOPHYSICAL JOURNAL

Near-Critical Fluctuations and Cytoskeleton-Assisted Phase Separation Lead to Subdiffusion in Cell Membranes

(2011) Jens Ehrig et al.   BIOPHYSICAL JOURNAL

Visualization and quantification of cardiac mitochondrial protein clusters with STED microscopy

(2011) Harpreet Singh et al.   MITOCHONDRION

Pre-existing clusters of the adaptor Lat do not participate in early T cell signaling events

(2011) David J Williamson et al.   NATURE IMMUNOLOGY

Probing protein heterogeneity in the plasma membrane using PALM and pair correlation analysis

(2011) Prabuddha Sengupta et al.   NATURE METHODS

Quantitative imaging of membrane lipid order in cells and organisms

(2011) Dylan M Owen et al.   Nature Protocols

Simultaneous multi-lifetime multi-color STED imaging for colocalization analyses

(2011) Johanna Bückers et al.   OPTICS EXPRESS

Nanoscale distribution of mitochondrial import receptor Tom20 is adjusted to cellular conditions and exhibits an inner-cellular gradient

(2011) C. A. Wurm et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Hierarchical mesoscale domain organization of the plasma membrane

(2011) Akihiro Kusumi et al.   TRENDS IN BIOCHEMICAL SCIENCES

Imaging Membrane Lipid Order in Whole, Living Vertebrate Organisms

(2010) Dylan M. Owen et al.   BIOPHYSICAL JOURNAL

Single-Molecule STED Microscopy with Photostable Organic Fluorophores

(2010) Robert Kasper et al.   Small

Detecting Protein Complexes in Living Cells from Laser Scanning Confocal Image Sequences by the Cross Correlation Raster Image Spectroscopy Method

(2009) Michelle A. Digman et al.   BIOPHYSICAL JOURNAL

Dynamic regulation of T-cell costimulation through TCR-CD28 microclusters

(2009) Tadashi Yokosuka et al.   IMMUNOLOGICAL REVIEWS

Actin-induced perturbation of PS lipid–cholesterol interaction: A possible mechanism of cytoskeleton-based regulation of membrane organization

(2009) S. Garg et al.   JOURNAL OF STRUCTURAL BIOLOGY

Structure and Dynamics of a Two-Helix SNARE Complex in Live Cells

(2009) Nagaraj D. Halemani et al.   TRAFFIC

Nanoclusters of GPI-Anchored Proteins Are Formed by Cortical Actin-Driven Activity

(2008) Debanjan Goswami et al.   CELL

Membrane heterogeneities in the formation of B cell receptor–Lyn kinase microclusters and the immune synapse

(2008) Hae Won Sohn et al.   JOURNAL OF CELL BIOLOGY

Direct observation of the nanoscale dynamics of membrane lipids in a living cell

(2008) Christian Eggeling et al.   NATURE

The Phasor Approach to Fluorescence Lifetime Imaging Analysis

(2007) Michelle A. Digman et al.   BIOPHYSICAL JOURNAL