Article
Urology & Nephrology
Isabelle Ayoub, Bethany J. Wolf, Linyu Geng, Huijuan Song, Aastha Khatiwada, Betty P. Tsao, Jim C. Oates, Brad H. Rovin
Summary: A machine learning approach combining traditional clinical data and novel urine biomarkers was used to develop prediction models for one-year treatment response in lupus nephritis. The models showed good predictive value, with the best performance seen in predicting eGFR response. The inclusion of both traditional clinical data and novel urine biomarkers, including cytokines, chemokines, and markers of kidney damage, was found to improve the prediction accuracy.
KIDNEY INTERNATIONAL
(2022)
Review
Immunology
Gongjun Tan, Binila Baby, Yuqiu Zhou, Tianfu Wu
Summary: Systemic lupus erythematosus (SLE) is a complex autoimmune disease with multifactorial etiology. The current diagnostic criteria for SLE have limited sensitivity and specificity, calling for the development of more accurate tools. Emerging omics technologies have potential in discovering biomarkers for SLE, but further validation is required.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Yves Renaudineau, Wesley Brooks, Julie Belliere
Summary: Lupus nephritis is the most severe organ manifestation of systemic lupus erythematosus. Efforts have been made to characterize the different steps of the disease and develop biomarkers for early diagnosis, disease monitoring, and preventing complications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Immunology
Jiaxuan Chen, Shuzhen Liao, Huimin Zhou, Lawei Yang, Fengbiao Guo, Shuxian Chen, Aifen Li, Quanren Pan, Chen Yang, Hua-feng Liu, Qingjun Pan
Summary: Animal models play a crucial role in understanding human diseases, but they cannot fully simulate the occurrence and progression of diseases due to genetic and disease-specific differences. Humanized immune system mice, developed from immunodeficient mice, provide a partial reconstruction of the human immune system and mimic the in vivo microenvironment. However, there are challenges in developing humanized mice models for systemic lupus erythematosus (SLE), including improving immune response reconstruction efficiency, extending observation period, and improving model development and utilization.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Pharmacology & Pharmacy
Desmond Yat Hin Yap, Chi Chiu Mok
Summary: This article summarizes new therapeutic approaches targeting B cells, T cells, crucial cytokines, and their signaling pathways in LN.
EXPERT REVIEW OF CLINICAL PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jordi Guiteras, Elena Crespo, Pere Fontova, Nuria Bolanos, Montse Goma, Esther Castano, Oriol Bestard, Josep M. Grinyo, Joan Torras
Summary: The study discovered a human fusion protein called Hybri, which effectively improves lupus nephritis by blocking the costimulatory pathway and exacerbating the coinhibitory pathway. This treatment method achieved good results in both lupus-prone models and severe lupus nephritis models, and also affected inflammatory-related cytokines and kidney gene expression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Wei Qijiao, Zhihan Chen, Panashe Makota, Yan Qing, Gao Fei, Zhihong Wang, Lin He
Summary: This study aimed to explore the molecular characteristics and mechanisms of lupus nephritis (LN) using bioinformatics analysis. The research identified several differentially expressed genes associated with inflammatory response and immune response, with upregulated expression of S100A8 in LN glomeruli. Additionally, the study found variations in glomerular S100A8 expression among different pathological types, indicating the need for further research to confirm its role in LN.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Marta Monticolo, Krzysztof Mucha, Bartosz Foroncewicz
Summary: Lupus nephritis is a common and serious complication of systemic lupus erythematosus, with genetic and environmental factors, including dysbiosis, playing a role in its development. The relationship between the human microbiome, genetic determinants, individual variability, and clinical consequences needs further investigation. Bacterial metabolites that mimic autoantigens and stimulate autoimmune responses may be a potential target for future interventions in Lupus nephritis.
Article
Immunology
Andrea R. Daamen, Hongyang Wang, Prathyusha Bachali, Nan Shen, Kathryn M. Kingsmore, Robert D. Robl, Amrie C. Grammer, Shu Man Fu, Peter E. Lipsky
Summary: This study characterized the molecular profiles of lupus nephritis (LN) development using gene expression analysis in lupus-prone NZM2328 mice. Different stages of LN exhibited distinct molecular characteristics, and it was found that NZM2328.R27 mice had resistance to chronic nephritis. The gene expression profiles in human LN were similar to those in NZM2328 mice, suggesting comparable disease stages.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Urology & Nephrology
Chris R. Toy, Huijuan Song, Haikady N. Nagaraja, Julia Scott, Jessica Greco, Xiaolan Zhang, Chack-Yung Yu, James A. Tumlin, Brad H. Rovin, Lee A. Hebert, Daniel J. Birmingham
Summary: The study reveals that the C5 gene's 2404-G allele/GG genotype is associated with lupus nephritis in black lupus patients and can lead to sharp increases in urine C5a and MAC levels during certain LN flares.
KIDNEY INTERNATIONAL REPORTS
(2021)
Review
Immunology
Mark Juha, Adel Molnar, Zoltan Jakus, Nora Ledo
Summary: Neutrophil extracellular traps (NETs) are web-like structures that prevent microbial dissemination but can also cause proinflammatory adverse effects in certain renal diseases. Understanding the pathogenesis and regulation of NETosis is crucial for developing targeted therapeutic options. This article provides a comprehensive review of NETosis in renal disorders and discusses current treatment strategies and molecular targets.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Jingquan He, Chiyu Ma, Donge Tang, Shaoyun Zhong, Xiaofang Yuan, Fengping Zheng, Zhipeng Zeng, Yumei Chen, Dongzhou Liu, Xiaoping Hong, Weier Dai, Lianghong Yin, Yong Dai
Summary: This study investigated the alteration of oxylipins in the serum of systemic lupus erythematosus (SLE) patients and identified potential biomarkers for SLE classification. The results showed that certain oxylipins were correlated with lupus nephritis (LN) and SLE disease activity. Additionally, a combination of 5 oxylipins was found to have high accuracy in classifying SLE patients. The findings provide insights into the characteristics of SLE and the development of new tools for classification.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Chen Yu, Ping Li, Xin Dang, Xuan Zhang, Yonghui Mao, Xiangmei Chen
Summary: This review focuses on lupus nephritis (LN), a common manifestation of systemic lupus erythematosus (SLE), and discusses the challenges in its diagnosis and treatment as well as the potential targets for future management.
JOURNAL OF AUTOIMMUNITY
(2022)
Article
Engineering, Biomedical
Qian Wu, Youmei Li, Lei Wang, Dong Wang, Ben Zhong Tang
Summary: This review discusses the recent advances in aggregation-induced emission (AIE) for brain imaging and therapy. AIE fluorophores, through rational design and functionalized strategies, demonstrate superior properties such as deep tissue penetration, high contrast to autofluorescence, and efficient blood-brain barrier crossing.
Article
Medicine, General & Internal
Kazuhiko Fukushima, Haruhito A. Uchida, Yasuko Fuchimoto, Tomoyo Mifune, Mayu Watanabe, Kenji Tsuji, Katsuyuki Tanabe, Masaru Kinomura, Shinji Kitamura, Yosuke Miyamoto, Sae Wada, Taisaku Koyanagi, Hitoshi Sugiyama, Takumi Kishimoto, Jun Wada
Summary: Close attention is needed, particularly for respiratory system events and infectious diseases, when treating patients with silica-associated SLE.
Article
Multidisciplinary Sciences
Daan C. t Hart, Johan van der Vlag, Tom Nijenhuis
Summary: Shear stress induced by laminar flow significantly impacts the morphology and functional phenotype of glomerular endothelial cells, leading to cytoskeletal rearrangement, increased nitric oxide production, enhanced expression of Heparan Sulfate, and a quiescent phenotype characterized by decreased expression of pro-inflammatory genes and increased expression of anti-oxidant enzymes. Additionally, glomerular endothelial cells cultured under laminar flow show an enhanced inflammatory response when exposed to the stimulus TNF alpha, suggesting a different response to shear stress compared to macrovascular endothelium.
Article
Biochemistry & Molecular Biology
Alexander H. van Asbeck, Jurgen Dieker, Rik Oude Egberink, Lennard van den Berg, Johan van der Vlag, Roland Brock
Summary: The study found a linear correlation between dose, uptake, and expression under different delivery vehicles, cell lines, and incubation times, and differences in expression levels can be explained by the number of mRNAs packaged per delivery vehicle. Using mRNA provides excellent quantitative control over protein expression over extended periods of time.
Review
Biochemistry & Molecular Biology
Maximilian Ackermann, Hans-Joachim Anders, Rostyslav Bilyy, Gary L. Bowlin, Christoph Daniel, Rebecca De Lorenzo, Mikala Egeblad, Timo Henneck, Andres Hidalgo, Markus Hoffmann, Bettina Hohberger, Yogendra Kanthi, Mariana J. Kaplan, Jason S. Knight, Jasmin Knopf, Elzbieta Kolaczkowska, Paul Kubes, Moritz Leppkes, Aparna Mahajan, Angelo A. Manfredi, Christian Maueroeder, Norma Maugeri, Ioannis Mitroulis, Luis E. Munoz, Teluguakula Narasaraju, Elisabeth Naschberger, Indira Neeli, Lai Guan Ng, Marko Z. Radic, Konstantinos Ritis, Patrizia Rovere-Querini, Mirco Schapher, Christine Schauer, Hans-Uwe Simon, Jeeshan Singh, Panagiotis Skendros, Konstantin Stark, Michael Stuerzl, Johan van der Vlag, Peter Vandenabeele, Ljubomir Vitkov, Maren von Koeckritz-Blickwede, Cansu Yanginlar, Shida Yousefi, Alexander Zarbock, Georg Schett, Martin Herrmann
Summary: SARS-CoV-2 infection poses a major threat to multiple organs, with severe cases showing dysregulated immune responses. The imbalance between neutrophil extracellular trap (NET) formation and degradation plays a central role in the pathophysiology of severe COVID-19 cases. Therapeutic strategies targeting dysregulated NET formation or degradation may benefit severe COVID-19 patients.
CELL DEATH AND DIFFERENTIATION
(2021)
Article
Oncology
Laura Miesen, Roy Wetzels, Jennifer Eymael, Fieke Mooren, Valerie Villacorta Monge, Bartholomeus T. van den Berge, Martijn van den Broek, Thea J. A. M. van der Velden, Lambertus P. W. J. van den Heuvel, Jack F. M. Wetzels, Michiel F. Schreuder, Johan van der Vlag, Jitske Jansen, Bart Smeets
Summary: PECs are epithelial cells in the kidney that play a crucial role in the formation of sclerotic lesions in FSGS. The development of a novel ciPEC cell line provides a valuable tool for future studies on the role of PECs in FSGS.
EXPERIMENTAL CELL RESEARCH
(2021)
Article
Endocrinology & Metabolism
Anouk I. M. van der Velden, Bernard M. van den Berg, Renee de Mutsert, Johan van der Vlag, J. Wouter Jukema, Frits R. Rosendaal, Ton J. Rabelink, Hans Vink
Summary: This study aimed to investigate microvascular differences in individuals with obesity at risk for developing cardiovascular disease. It found that the high-risk group had significant reductions in the number of smallest capillaries and higher red blood cell velocity in feed vessels compared to the low-risk group. The study concluded that sidestream dark field imaging, combined with red blood cell velocity, can detect microcirculatory differences in individuals with obesity at risk for cardiovascular disease.
Article
Multidisciplinary Sciences
Rachel P. L. van Swelm, Sanne Beurskens, Henry Dijkman, Erwin T. G. Wiegerinck, Rian Roelofs, Frank Thevenod, Johan van der Vlag, Jack F. M. Wetzels, Dorine W. Swinkels, Bart Smeets
Summary: This study investigated the molecular mechanisms of kidney iron loading in an experimental model of focal segmental glomerulosclerosis (FSGS) and found that kidney iron deposition coincides with the progression of tubulointerstitial injury. Reduction of iron loading can halt the disease progression.
SCIENTIFIC REPORTS
(2022)
Article
Developmental Biology
Jitske Jansen, Bartholomeus T. van den Berge, Martijn van den Broek, Rutger J. Maas, Deniz Daviran, Brigith Willemsen, Rona Roverts, Marit van der Kruit, Christoph Kuppe, Katharina C. Reimer, Gianluca Di Giovanni, Fieke Mooren, Quincy Nlandu, Helmer Mudde, Roy Wetzels, Dirk den Braanker, Naomi Parr, James S. Nagai, Vedran Drenic, Ivan G. Costa, Eric Steenbergen, Tom Nijenhuis, Henry Dijkman, Nicole Endlich, Nicole C. A. J. van de Kar, Rebekka K. Schneider, Jack F. M. Wetzels, Anat Akiva, Johan van der Vlag, Rafael Kramann, Michiel F. Schreuder, Bart Smeets
Summary: Using organoid podocytes as a model, this study investigated idiopathic nephrotic syndrome and found that repaired organoids exhibited improved functionality, providing a valuable tool for disease modeling and therapy development.
Review
Immunology
Nils Rother, Cansu Yanginlar, Elmar Pieterse, Luuk Hilbrands, Johan van der Vlag
Summary: This article introduces the main techniques for isolating and characterizing microparticles, explains the characteristics of microparticles generated during cell death, illustrates the different mechanisms of intercellular communication through microparticles, and summarizes the role of microparticles in pathological mechanisms, with a particular focus on autoimmune diseases.
FRONTIERS IN IMMUNOLOGY
(2022)
Editorial Material
Urology & Nephrology
Rutger J. Maas, Tom Nijenhuis, Johan van der Vlag
KIDNEY INTERNATIONAL REPORTS
(2022)
Article
Immunology
Carolin Christina Drost, Alexandros Rovas, Irina Osiaevi, Matthias Rauen, Johan van der Vlag, Baranca Buijsers, Rustem Salmenov, Alexander Lukasz, Hermann Pavenstaedt, Wolfgang A. Linke, Philipp Kuempers
Summary: COVID-19 is associated with eGC damage, and excess activity of HPSE in COVID-19 patients is correlated with eGC damage. In translational experiments, changes in eGC thickness of cells exposed to COVID-19 serum are closely correlated with HPSE activity in corresponding plasma samples. Inhibition of HPSE by a nonanticoagulant heparin fragment prevents eGC injury, suggesting a potential protective effect of heparin in COVID-19 due to its effect on eGC.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Dirk den Braanker, Rutger Maas, Naomi Parr, Jeroen Deegens, Bart Smeets, Jack Wetzels, Johan van der Vlag, Tom Nijenhuis
Summary: In this study, a Thy-1.1 transgenic mouse model prone to FSGS was developed to accelerate proteinuria after injection of samples from FSGS patients. It was found that different genetic backgrounds in mice resulted in varying onset and severity of proteinuria. The experiment demonstrated that presumably CPF-containing plasmapheresis effluent induced proteinuria, but not serum. This model can be used to study CPFs, but lacks sensitivity for predicting FSGS recurrence in a clinical setting.
Article
Biochemistry & Molecular Biology
Dirk J. W. den Braanker, Rutger J. H. Maas, Guido van Mierlo, Naomi M. J. Parr, Marinka Bakker-van Bebber, Jeroen K. J. Deegens, Pascal W. T. C. Jansen, Jolein Gloerich, Brigith Willemsen, Henry B. B. Dijkman, Alain J. J. van Gool, Jack F. M. Wetzels, Markus M. M. Rinschen, Michiel Vermeulen, Tom Nijenhuis, Johan van der Vlag
Summary: This study aimed to investigate the possible factors and mechanisms leading to recurrence of focal segmental glomerulosclerosis (FSGS) after kidney transplantation. It was found that a protein called perilipin-2 was significantly increased in infiltrating cells of patients' glomeruli and was associated with lipid droplet accumulation, suggesting it as a potential biomarker. Further research should focus on understanding how these factors alter lipid metabolism in podocytes and provide new treatment strategies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Jennifer Eymael, Martijn van den Broek, Laura Miesen, Valerie Villacorta Monge, Bartholomeus T. van den Berge, Fieke Mooren, Vicky Luna Velez, Jelmer Dijkstra, Meyke Hermsen, Peter Bandi, Michiel Vermeulen, Saskia de Wildt, Brigith Willemsen, Sandrine Florquin, Roy Wetzels, Eric Steenbergen, Rafael Kramann, Marcus Moeller, Michiel F. Schreuder, Jack Fm Wetzels, Johan van der Vlag, Jitske Jansen, Bart Smeets
Summary: Scattered tubular cells (STCs) in the proximal tubule increase in number after acute kidney injury. STCs are preferentially located within the inner bends of the tubule and their number increases with age. STCs represent a transient state of dedifferentiated proximal tubule epithelial cells (PTECs) showing metabolic shift towards glycolysis, facilitating cellular survival after kidney injury.
JOURNAL OF PATHOLOGY
(2023)
Meeting Abstract
Medicine, General & Internal
C. Drost, A. Rovas, I Osiaevi, M. Rauen, J. Van der Vlag, B. Buijsers, P. Kuempers
MEDIZINISCHE KLINIK-INTENSIVMEDIZIN UND NOTFALLMEDIZIN
(2022)
Article
Cell Biology
Jennifer Eymael, Brigith Willemsen, Joyce Xu, Fieke Mooren, Eric Steenbergen, Jack F. Wetzels, Henry Dijkman, Jitske Jansen, Johan van der Vlag, Bart Smeets
Summary: Multiple motile cilia with a central microtubular pair were found in kidney biopsies of patients with kidney diseases, and these cells were associated with tubular injury and interstitial fibrosis. This suggests the ability of proximal tubular cells to transdifferentiate into multiciliated cells.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)