Article
Biochemistry & Molecular Biology
Kunpeng Liu, Dongbo Qiu, Xue Liang, Yingqi Huang, Jingyuan Zhao, Xiusheng Qiu, Qi Zhang, Zhen-Dong Xiao, Yunfei Qin
Summary: Type I interferons are crucial for host defense against viral infections and posttranslational modifications, including ubiquitination and deubiquitination, regulate their signaling. While many ubiquitin ligase enzymes have been implicated in antiviral responses, the role of deubiquitinating enzymes in this process remains poorly understood. This study investigated the expression and function of DUB genes in regulating antiviral signaling in THP-1 cells, suggesting their involvement in host antiviral activities.
MOLECULAR IMMUNOLOGY
(2021)
Article
Fisheries
Fei Han, Yuan Zhang, Na Song, Tianxiang Gao
Summary: This study focused on the burbot fish and characterized 19 Toll-like receptor (TLR) genes in it, examining their expression patterns after pathogen exposure. The researchers found that TLR genes, essential in the fish's innate immune system, were tandemly repeated, indicating an evolution in the fish's immune system. Different TLR subfamilies showed tissue-specific expressions, and the response of TLR genes to pathogen exposure was also investigated. The results revealed time and tissue-specific expression patterns and highlighted the crucial role of TLR genes in the burbot's immune response.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Dandan Zhao, Anh Thi Tram Tu, Miwako Shobo, Nguyen Bui Thao Le, Chiaki Yoshikawa, Kazuhisa Sugai, Yoji Hakamata, Tomohiko Yamazaki
Summary: In this study, the ε-PLL/G4-CpG ODN complex was successfully synthesized as a carrier, providing better stability against nucleases and significantly improved cellular uptake. Modulation of the N/P ratio successfully tuned the levels of immune cytokine production.
Article
Environmental Sciences
Lei Liu, Di Wang, Yang Fu, Zhongyi Duan, Adeyinka Abiola Adetula, Huagui Liu, Ying Yu, Qin Chu
Summary: Poly(I:C) maternal stimulation affects gene expression patterns in laying hens and their offspring, indicating an intergenerational transmission effect. Functional enrichment analysis shows that Poly(I:C) may affect the immunity of the treated hens and the subsequent unchallenged generations. Pathways associated with growth, development, biosynthesis, and metabolism of F2 chicks are also affected by Poly(I:C) maternal stimulation.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Neurosciences
Daniel Bormann, Dragan Copic, Katharina Klas, Martin Direder, Christian J. Riedl, Giulia Testa, Hannes Kuehtreiber, Emilia Poreba, Simon Hametner, Bahar Golabi, Melanie Salek, Carmen Haider, Verena Endmayr, Lisa E. Shaw, Romana Hoeftberger, Hendrik J. Ankersmit, Michael Mildner
Summary: This study investigated the acute responses of the mammalian brain to bacterial and viral PAMP challenges. It found shared and distinct responses in brain vascular and ependymal cells, limited neuronal response to PAMPs, and a pronounced dependency of oligodendrocytes on IFN stimulation during viral PAMP challenges.
NEUROBIOLOGY OF DISEASE
(2023)
Review
Cell Biology
Kieran Maheden, Vivian Weixuan Zhang, Nika Shakiba
Summary: Stem cells face selective pressures that lead to the dominance of certain clones. Cell competition is a conserved mechanism to counteract these pressures. It has been observed in various mammalian tissue systems, but there are challenges in terms of terminology and capturing information flow. The use of next generation technologies can help bridge these gaps and further our understanding of cell competition.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Qiu-Ning Liu, Ying-Yu Tang, Meng-Jiao Zhou, Sha Luo, Yue-Tian Li, Gang Wang, Dai-Zhen Zhang, Hui Yang, Bo-Ping Tang, Wen-Fei He
Summary: This study analyzed the transcriptomic profiles of spleens from Tachysurus fulvidraco treated with poly (I:C), revealing significant dysregulation of multiple immune- and cancer-related genes. The findings suggest that poly (I:C) may represent a promising adjuvant for fish vaccines.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Virology
Haiyun Liu, Zhidong Teng, Hu Dong, Jiajun Li, Sahibzada Waheed Abdullah, Yun Zhang, Jinen Wu, Huichen Guo, Shiqi Sun
Summary: This study used CpG and Poly (I:C) as scaffolds to facilitate the assembly of FMDV VLPs in vitro, successfully encapsulating CpG and Poly (I:C) inside the VLPs and improving the assembly efficiency and immune response of the VLPs.
Article
Immunology
Xin Zhao, Ruqayah Mohammed, Hieu Tran, Mary Erickson, Amanda C. Kentner
Summary: The study investigated the effects of maternal immune activation (MIA) on social behavior and HPA axis functioning in mice, and found that enrichment housing could mitigate these effects by improving maternal care. The data suggest that environmental augmentation supports parental care and offspring safety/security, offsetting the effects of early health adversity by buffering HPA axis dysregulation.
BRAIN BEHAVIOR AND IMMUNITY
(2021)
Article
Chemistry, Multidisciplinary
Silvia Galvan, Oliver Madderson, Shuai Xue, Ana P. Teixeira, Martin Fussenegger
Summary: Next-generation gene and engineered-cell therapies benefit from incorporating synthetic gene networks that can precisely regulate therapeutic output. A mammalian gene switch, responsive to xylose, has been designed and successfully tested in mice. The switch consists of a fusion protein that activates transgene expression in a dose-dependent manner in the presence of xylose. By implanting SWEET-engineered cells, increased levels of cargo protein were observed in mice when consuming xylose-sweetened water or coffee, indicating the potential of this therapy to integrate into patients' lifestyles and dietary habits. In a proof-of-concept study, diabetic mice with insulin-expressing SWEET showed improved glycemia and increased insulin levels when administered this diabetic-compliant sweetener.
Review
Nutrition & Dietetics
Cristian Sandoval, Karen Nahuelqueo, Luciana Mella, Blanca Recabarren, Vanessa Souza-Mello, Jorge Farias
Summary: This comprehensive review aims to elucidate the involvement of long-chain polyunsaturated fatty acids, specifically eicosapentaenoic acid and docosahexaenoic acid, in the modulation of gene expression during the progression of obesity. The findings suggest that the incorporation of eicosapentaenoic and docosahexaenoic acid may have potential advantages and efficacy in addressing noncommunicable diseases, including obesity, due to their anti-inflammatory properties and their ability to regulate genes associated with obesity.
FRONTIERS IN NUTRITION
(2023)
Article
Biochemistry & Molecular Biology
Teresa Improda, Valentina Morgera, Maria Vitale, Lorenzo Chiariotti, Fabiana Passaro, Antonia Feola, Antonio Porcellini, Mariella Cuomo, Antonio Pezone
Summary: Cell identity is determined by chromatin structure and gene expression profiles, which rely on chromatin accessibility and DNA methylation. These epigenetic modifications are crucial for mammalian development and cellular identity. Dynamic DNA methylation and demethylation occur during cell fate commitment and terminal differentiation. By analyzing the methylation configurations of gene promoters during murine brain differentiation, we found significant methyl-CpG profiles associated with gene silencing or activation. Interestingly, these methylation patterns mark different brain areas and cell types during differentiation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Hiroki Makanai, Tatsuya Nishihara, Makiya Nishikawa, Kazuhito Tanabe
Summary: In this study, modified ODNs with Hoechst molecules were successfully designed and characterized for accumulation and transport into the cell nucleus.
Article
Genetics & Heredity
Jianlu Zhang, Jiqin Huang, Haitao Zhao
Summary: In this study, the expression and tissue distribution of TLR2 and TLR4 in Schizothorax prenanti were investigated. It was found that both TLR2 and TLR4 were widely expressed in various tissues, especially immune-related tissues. Injection of poly (I:C) significantly upregulated the expression of these proteins, indicating their potential roles in antiviral immunity and pathological processes in S. prenanti.
Article
Dentistry, Oral Surgery & Medicine
B. He, V. Kram, T. Furusawa, O. Duverger, E. Y. Chu, R. Nanduri, M. Ishikawa, P. Zhang, B. A. Amendt, J. S. Lee, M. Bustin
Summary: The study suggests that HMGN proteins play a role in regulating ameloblast differentiation and enamel mineralization by modulating lineage-specific chromatin accessibility and transcription factor binding to ameloblast regulatory sites. The absence of HMGN proteins in mice leads to faster ameloblast differentiation and increased enamel deposition rate in molars and incisors.
JOURNAL OF DENTAL RESEARCH
(2023)
Article
Cell Biology
Tobias Hilbert, Paul Markowski, Stilla Frede, Olaf Boehm, Pascal Knuefermann, Georg Baumgarten, Andreas Hoeft, Sven Klaschik
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2018)
Article
Chemistry, Multidisciplinary
Stefan Lorscheidt, Maryam A. Shetab Boushehri, Sven Klaschik, Alf Lamprecht
JOURNAL OF CONTROLLED RELEASE
(2019)
Article
Cell Biology
Sven Klaschik, Jennifer Gehlen, Claudia Neumann, Mignon-Denise Keyver-Paik, Martin Soehle, Stilla Frede, Markus Velten, Andreas Hoeft, Tobias Hilbert
MEDIATORS OF INFLAMMATION
(2019)
Article
Medicine, General & Internal
Josefin Grabert, Sven Klaschik, Agi Gueresir, Patrick Jakobs, Martin Soehle, Hartmut Vatter, Tobias Hilbert, Erdem Gueresir, Markus Velten
Article
Medicine, Research & Experimental
Jennifer Gehlen, Sven Klaschik, Claudia Neumann, Mignon-Denise Keyver-Paik, Alexander Mustea, Martin Soehle, Stilla Frede, Markus Velten, Andreas Hoeft, Tobias Hilbert
JOURNAL OF TRANSLATIONAL MEDICINE
(2020)
Article
Anesthesiology
B. Bette, M. Martini, S. Klaschik, T. Hilbert
Article
Medicine, Research & Experimental
Marcel Scheck, Markus Velten, Sven Klaschik, Martin Soehle, Stilla Frede, Jennifer Gehlen, Jochen Hoch, Alexander Mustea, Andreas Hoeft, Tobias Hilbert
Article
Medicine, General & Internal
Claudia Neumann, Markus Velten, Cornelia Heik-Guth, Brigitte Strizek, Maria Wittmann, Tobias Hilbert, Sven Klaschik
Article
Medicine, Research & Experimental
Alexander Kinnen, Sven Klaschik, Claudia Neumann, Eva-Katharina Egger, Alexander Mustea, Martin Soehle, Stilla Frede, Markus Velten, Mark Coburn, Tobias Hilbert
Summary: The study found that ovarian cancer patients received more fluid intraoperatively, with increased expression of genes like IL-8 and MCP-1/CCL2 in their serum. Significant alterations in vascular leakage-related genes were detected in tumor tissue of ovarian cancer patients, and the ANG-2/1 expression ratio was significantly higher in tumor-free peritoneal tissue. Changes in gene expression in peripheral tissues of ovarian cancer patients were associated with postoperative fluid balance.
Article
Anesthesiology
Konrad Peukert, Sven Klaschik, Tobias Hilbert
Article
Biochemistry & Molecular Biology
Jan Menzenbach, Stilla Frede, Janine Petras, Vera Guttenthaler, Andrea Kirfel, Claudia Neumann, Andreas Mayr, Maria Wittmann, Mark Coburn, Sven Klaschik, Tobias Hilbert
Summary: Preoperative serum biomarker profiling with specificity for vascular dysfunction and systemic inflammation was not indicative of subsequent POD development. Surgery-induced systemic inflammation, particularly following cardiac surgery, was associated with POD.
Article
Anesthesiology
Magdalena Sitter, Tobias Schlesinger, Ann-Kristin Reinhold, Axel Scholler, Christian von Heymann, Sabine Welfle, Catharina Bartmann, Achim Woeckel, Stefan Kleinschmidt, Sven Schneider, Andre Gottschalk, Susanne Greve, Julius Z. Wermelt, Roland Wiener, Frank Schulz, Daniel Chappell, Maya Brunner, Claudia Neumann, Patrick Meybohm, Peter Kranke
Summary: This study provides an overview of SARS-CoV-2 positive pregnant women hospitalized for childbirth in Germany and Switzerland during the pandemic. The findings show that the number of pregnant women infected with SARS-CoV-2 at the time of delivery was very low, with most cases presenting as asymptomatic or mild, but also demonstrating the potential for severe progression even in young healthy patients.
Article
Anesthesiology
Claudia Neumann, Tamara Babasiz, Nadine Strassberger-Nerschbach, Ehrenfried Schindler, Christian Reuter, Leonie Weinhold, Maria Wittmann, Tobias Hilbert, Sven Klaschik
Summary: This study aimed to evaluate the validity of the Newborn Infant Parasympathetic Evaluation (NIPE) index in assessing nociception during anesthesia in children below 2 years. The results showed that the NIPE index was more reliable than heart rate in indicating nociception. However, its impact on postoperative outcome remains unclear.
PEDIATRIC ANESTHESIA
(2022)
Article
Orthopedics
Alina Yurutkina, Sven Klaschik, Pascal Kowark, Annette Gass, Carolina Link, Thomas Martin Randau, Jorge Jimenez-Cruz, Mark Coburn, Tobias Hilbert
Summary: This study retrospectively analyzed the comparison of analgesic methods in patients undergoing hip and knee arthroplasty. The results showed that after hip arthroplasty, WPD PCA provided better pain relief and patient comfort, while after knee arthroplasty, Pir PCA provided superior analgesia and a lower complication rate.
JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH
(2022)
Article
Medicine, Research & Experimental
Eva Katharina Egger, Natascha Kohls, Matthias B. Stope, Mateja Condic, Mignon-Denise Keyver-Paik, Dominique Koensgen, Tobias Hilbert, Sven Klaschik, Daniel Exner, Tim Vilz, Alexander Mustea
