Article
Microbiology
Farha Deeba, Kukkala Kiran Kumar, Girish H. Rajacharya, Naseem A. Gaur
Summary: The study investigated the metabolomic adaptations of oleaginous yeast Rhodosporidium toruloides in response to varying glucose and nitrogen concentrations. Under specific glucose and nitrogen conditions, the biomass and lipid production were found to be maximum in the yeast.
Article
Biochemical Research Methods
Jorge Oliveira, Miguel Antunes, Claudia P. Godinho, Miguel C. Teixeira, Isabel Sa-Correia, Pedro T. Monteiro
Summary: The article introduces a new method to download and organize genome information from NCBI in GenBank format with a set of simple commands. This method allows comparison of new genomes with reference genomes to find homologous genes and predict transcription associations. The tool is demonstrated to be useful in analyzing the genome of the oleaginous red yeast species Rhodotorula toruloides, highlighting its potential impact on optimizing yeast robustness for industrial bioconversion processes.
BMC BIOINFORMATICS
(2021)
Article
Agricultural Engineering
Zhijia Liu, Mohammad Radi, Elsayed T. T. Mohamed, Adam M. Feist, Giuliano Dragone, Solange I. Mussatto
Summary: The use of adaptive laboratory evolution in hydrolysate-based medium improved the tolerance of Rhodosporidium toruloides to inhibitors in lignocellulosic biomass hydrolysate, resulting in enhanced lipid accumulation and carotenoid production compared to the wild-type strain. Whole genome sequencing revealed that the wild-type strain naturally contained tolerance-related genes that facilitated evolution in biomass-derived inhibitors.
BIORESOURCE TECHNOLOGY
(2021)
Article
Agricultural Engineering
Yuwei Chen, Feng Long, Qitian Huang, Kui Wang, Jianchun Jiang, Jie Chen, Junming Xu, Xiaoan Nie
Summary: Hydrothermal liquefaction (HTL) with acidic ionic liquid [Bmim][HSO4] as catalyst enables direct and efficient conversion of oleaginous yeast Rhodosporidium toruloides to biodiesel, yielding high biodiesel content with high heating value.
BIORESOURCE TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Sujit Sadashiv Jagtap, Anshu Deewan, Jing-Jing Liu, Hanna E. Walukiewicz, Eun Ju Yun, Yong-Su Jin, Christopher V. Rao
Summary: This study investigated the central carbon metabolism in Rhodosporidium toruloides during growth on different substrates using transcriptomics and metabolomics. Significant changes in gene expression and metabolite concentrations were observed, with a notable finding in xylose metabolism. These results enhance understanding of R. toruloides metabolism and may guide metabolic engineering and model development for this organism.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Agricultural Engineering
Xuehai Zheng, Rongfei Hu, Duo Chen, Jiannan Chen, Wenjin He, Luqiang Huang, Chentao Lin, Huibin Chen, Youqiang Chen, Jinmao Zhu, Feng Qi, Ting Xue
Summary: The addition of cane molasses at concentrations of 35 g/L and 70 g/L significantly increased lipid and carotenoid production in the microalgae R. toruloides M18, demonstrating a cost-effective and environmentally friendly strategy for enhancing production of these valuable compounds.
BIORESOURCE TECHNOLOGY
(2021)
Article
Environmental Sciences
Sangeeta Singh, Tanmay Bharadwaj, Devendra Verma, Kasturi Dutta
Summary: The study found that the oleaginous yeast Rhodosporidium toruloides 9564(T) can degrade phenol and produce lipids. Increasing the inoculum size can overcome the toxicity of phenol. The results indicate that using industrial wastewater for bioenergy production can promote the circular economy.
Article
Biochemistry & Molecular Biology
Khanh Dung Pham, Yuuki Hakozaki, Takeru Takamizawa, Atsushi Yamazaki, Harutake Yamazaki, Kazuki Mori, Sachiyo Aburatani, Kosuke Tashiro, Satoru Kuhara, Hiroaki Takaku, Yosuke Shida, Wataru Ogasawara
Summary: Light stimulates carotenoid production in an oleaginous yeast by promoting carotenoid biosynthesis genes through a two-step transcriptional activation process. CRY1 is suggested to play a role in this mechanism. Mutants with varying carotenoid production were generated to further understand the light response, leading to the proposal of a two-step gene activation control. Data analysis also indicates the likelihood of R. toruloides NBRC 10032 being a homo-diploid strain.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Stefano Bertacchi, Chiara Cantu, Danilo Porro, Paola Branduardi
Summary: This study focuses on extracting carotenoids from Camelina sativa meal using the yeast Rhodosporidium toruloides. By optimizing enzymatic and biomass loadings, carotenoids were obtained in similar quantities through separate or simultaneous fermentation and hydrolysis processes in stirred-tank bioreactors. The data obtained pave the way for potential future industrialization of this bioprocess, indicating the robustness and scalability of the process.
FERMENTATION-BASEL
(2021)
Article
Biotechnology & Applied Microbiology
Liting Lyu, Yadong Chu, Sufang Zhang, Yue Zhang, Qitian Huang, Shuang Wang, Zongbao K. Zhao
Summary: Researchers have developed engineered strains of Rhodosporidium toruloides by overexpressing genes responsible for phenolic compound degradation, leading to improved resistance to typical phenolic inhibitors and increased lipid production and cell growth in concentrated mode biomass hydrolysates.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Tuyet Nhung Tran, Ngoc-Tri Tran, Thu-Anh Tran, Dinh-Chuong Pham, Chia-Hung Su, Hoang Chinh Nguyen, Colin J. Barrow, Dai-Nghiep Ngo
Summary: This study aimed to produce astaxanthin from molasses using a newly mutated strain of Rhodosporidium toruloides G17 and evaluate its biological activities. By optimizing the culture conditions, a maximum astaxanthin yield of 1262.08 +/- 14.58 µg/L was achieved. The purified astaxanthin exhibited high antioxidant and anticancer activities, with 123-fold higher antioxidant activity than alpha-tocopherol and potent inhibitory effects against HeLa, A549, and MCF7 cancer cell lines.
FERMENTATION-BASEL
(2023)
Article
Biotechnology & Applied Microbiology
Di Zhao, Qidou Gao, Xiaochun Zheng, Sasa Liu, Qingsheng Qi, Xue Wang, Xiaobing Yang
Summary: Potential factors influencing the production of limonene in engineered strains were investigated through single-factor and orthogonal experiments. The optimized conditions included using MM as the medium, a working volume of 50 mL, inoculum OD600 = 0.6, 22°C incubation temperature, pH 6, and a dodecane coverage of 20%. The final limonene titer was improved from 52.5 to 358.1 mg/L, showing a 586% increase in titer and a 34.8-fold improvement in production efficiency (mg/OD600) in 250-mL flasks. These results suggest that R. toruloides could be a promising producer of limonene and facilitate the biosynthesis study of other terpenes.
FERMENTATION-BASEL
(2023)
Article
Biotechnology & Applied Microbiology
Shiyu Liang, Yue Zhang, Liting Lyu, Shuang Wang, Zongbao K. Zhao
Summary: A recombinant beta-1,3-glucomannanase, MAN5C, was introduced into the genome of the red yeast Rhodosporidium toruloides, enabling the secretion of active MAN5C and simplifying the lipid extraction process, resulting in a higher lipid recovery rate.
BIORESOURCES AND BIOPROCESSING
(2023)
Article
Biotechnology & Applied Microbiology
Carlos S. Osorio-Gonzalez, Rahul Saini, Krishnamoorthy Hegde, Satinder Kaur Brar, Alain Lefebvre, Antonio Avalos Ramirez
Summary: The effect of high sugar concentrations and ammonium sulfate on the growth and lipid accumulation of Rhodosporidium toruloides-1588 using undetoxified wood hydrolysates was studied. Batch fermentations showed that growth of R. toruloides-1588 was inhibited at sugar concentrations of 120 g/L, while the maximum lipid accumulation was 8.2 g/L with palmitic, stearic, oleic, linoleic, and lignoceric acids as the predominant fatty acids. Additionally, R. toruloides-1588 was able to utilize up to 80% of the total xylose content, which is the first report on the effect of using high xylose concentrations on the growth, sugar utilization, and lipid accumulation by R. toruloides-1588.
FERMENTATION-BASEL
(2023)
Article
Agricultural Engineering
Carlos S. Osorio-Gonzalez, Rahul Saini, Krishnamoorthy Hegde, Satinder K. Brar, Antonio Avalos Ramirez
Summary: This study observed the ability of Rhodosporidium toruloides-1588 to grow and accumulate lipids using furfural as a carbon source in media without glucose. The strain degraded furfural and produced other compounds while accumulating lipids. This finding provides a potential method for utilizing furfural as a renewable carbon source.
BIORESOURCE TECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ruibing Chen, Jiaoqi Gao, Wei Yu, Xianghui Chen, Xiaoxin Zhai, Yu Chen, Lei Zhang, Yongjin J. Zhou
Summary: Advances in synthetic biology enable efficient and safe synthesis of valuable natural products by microbial hosts. However, the role of cofactors in microbial production of natural products has been largely ignored. In this study, the supply and recycling of cofactors in yeast cells were engineered to achieve high-level production of phenolic acids.
NATURE CHEMICAL BIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Chunxiao Yan, Wei Yu, Xiaoxin Zhai, Lun Yao, Xiaoyu Guo, Jiaoqi Gao, Yongjin J. Zhou
Summary: This study systematically characterized native promoters in Ogataea polymorpha and obtained a group of constitutive, inducible, and hybrid promoters, which enriched the promoter toolbox for metabolic engineering of O. polymorpha and provided an important resource for its bio-manufacturing.
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY
(2022)
Correction
Biochemistry & Molecular Biology
Ruibing Chen, Jiaoqi Gao, Wei Yu, Xianghui Chen, Xiaoxin Zhai, Yu Chen, Lei Zhang, Yongjin J. Zhou
NATURE CHEMICAL BIOLOGY
(2022)
Article
Agricultural Engineering
Wei Yu, Xuan Cao, Jiaoqi Gao, Yongjin J. Zhou
Summary: In this study, the production efficiency of 3-HP was improved by optimizing gene expression and providing sufficient precursor and cofactor supply. The highest 3-HP production from glucose was achieved.
BIORESOURCE TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Kun Zhang, Xingpeng Duan, Peng Cai, Linhui Gao, Xiaoyan Wu, Lun Yao, Yongjin J. Zhou
Summary: This study demonstrates that fusion expression of the endogenous exonuclease Mre11 with Cas9 enhances homologous recombination efficiency in P. pastoris. This strategy facilitates the metabolic engineering of P. pastoris for high-level production of value-added compounds.
MICROBIAL CELL FACTORIES
(2022)
Article
Biotechnology & Applied Microbiology
Rui Hou, Linhui Gao, Jianhui Liu, Zhen Liang, Yongjin J. Zhou, Lihua Zhang, Yukui Zhang
Summary: This study conducted a label-free quantitative proteomic analysis of the methanol metabolism in methylotrophic yeast and identified the highly up-regulated expression of the transaldolase isoenzyme, which plays a crucial role in improving methanol biotransformation efficiency in Pichia pastoris.
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Ding-Wei Ji, Yan-Cheng Hu, Xiang-Ting Min, Heng Liu, Wei-Song Zhang, Ying Li, Yongjin J. Zhou, Qing-An Chen
Summary: This study demonstrates a unique skeleton-reorganizing coupling reaction of cycloheptatriene and cycloalkenones with amines. The reaction selectively couples cycloheptatriene with anilines to deliver fused 1,2-dihydroquinoline products in the presence of Rh/acid catalysis. Mechanistic studies reveal that the ring reorganization occurs through retro-Mannich type ring-opening and subsequent intramolecular Povarov reaction. The synthetic utilization of this reaction is showcased with gram-scale reaction, synthetic derivatizations, and late-stage modification of commercial drugs.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Biochemical Research Methods
Chunxiao Yan, Ning Gao, Xuan Cao, Lun Yao, Yongjin J. Zhou, Jiaoqi Gao
Summary: This study found that the deficiency of amino acids and nucleotides has effects on cell growth and product synthesis in yeast. These effects can be restored by adding appropriate supplementation of amino acids or nucleotides.
BIOTECHNOLOGY JOURNAL
(2023)
Article
Biotechnology & Applied Microbiology
Xuan Cao, Wei Yu, Yu Chen, Shan Yang, Zongbao K. Zhao, Jens Nielsen, Hongwei Luan, Yongjin J. Zhou
Summary: This study used a modular approach to globally rewire the cellular metabolism and achieved a sclareol production of 11.4 g/L, the highest reported titer in microbes. Metabolic flux analysis showed that modular balanced metabolism directed the metabolic flux towards the biosynthesis of targeted molecules, and transcriptomic analysis revealed that the expression of central metabolism genes was shaped for a new balanced metabolism, which laid a foundation for extensive metabolic engineering of other microbial species for sustainable bio-production.
METABOLIC ENGINEERING
(2023)
Article
Biotechnology & Applied Microbiology
Min Ye, Jiaoqi Gao, Yongjin J. Zhou
Summary: Bioproduction of natural products using microbial cell factories is a promising alternative to traditional plant extraction. In this study, the cellular metabolism of Ogataea polymorpha was rewired to achieve high-level production of the sesquiterpenoid beta-elemene. By optimizing the mevalonate pathway, enhancing the supply of NADPH and acetylCoA, and downregulating competitive pathways, the engineered strain produced significant amounts of beta-elemene. This research highlights the potential industrial application of O. polymorpha as a microbial platform for producing sesquiterpenoids.
METABOLIC ENGINEERING
(2023)
Article
Chemistry, Multidisciplinary
Xiaoyan Wu, Peng Cai, Linhui Gao, Yunxia Li, Lun Yao, Yongjin J. Zhou
Summary: Genetically modified industrial yeast Pichia pastoris was engineered to efficiently produce 3-hydroxypropionic acid (3-HP) from methanol. Through optimization of gene expression, substrate supply, and NADPH availability, 48.2 g/L of 3-HP was obtained in large-scale bioreactor cultivation. This study highlights the potential of P. pastoris for methanol biotransformation and provides strategies for the production of other acetyl-CoA derivatives from methanol.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2023)
Article
Agriculture, Multidisciplinary
Chunyang Cao, Haiyan Zhang, Xuan Cao, Sijia Kong, Beiwei Zhu, Xinping Lin, Yongjin J. Zhou
Summary: Engineering microorganisms to synthesize isoprenoids is an efficient approach to increase their concentration. In this study, three types of isoprenoid pathways were constructed and optimized in yeast peroxisomes to produce the sesquiterpene (+)-valencene. The Haloarchaea-type MVA pathway was found to be more effective, and a production of 869 mg/L (+)-valencene was achieved. This work expands isoprenoid synthesis in eukaryotes and provides a more efficient pathway for isoprenoid production.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Jiaoqi Gao, Wei Yu, Yunxia Li, Mingjie Jin, Lun Yao, Yongjin J. Zhou
Summary: Engineered yeast Ogataea polymorpha has been used to co-utilize glucose and xylose for the production of free fatty acids (FFAs) and 3-hydroxypropionic acid (3-HP) from lignocellulose.
NATURE CHEMICAL BIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Shujing Qiao, Fan Bai, Peng Cai, Yongjin J. Zhou, Lun Yao
Summary: An improved CRISPRi system in Pichia pastoris has been developed by fusing dCas9 with endogenous transcriptional repressor domains. This system shows strong repression of eGFP gene, with the highest efficiency of 85%. The gRNA expression and processing play a critical role in CRISPRi efficiency, and Csy4 endoribonuclease shows higher repression efficiency.
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY
(2023)
Article
Chemistry, Applied
Wei Yu, Jiaoqi Gao, Lun Yao, Yongjin J. Zhou
Summary: Metabolic engineering of industrial methylotrophic yeast Ogataea polymorpha was conducted to overproduce 3-hydroxypropionate (3-HP), an important platform chemical that can be used for the production of various special chemicals.
CHINESE JOURNAL OF CATALYSIS
(2023)
