Editorial Material
Biochemical Research Methods
Leonard J. Foster
Summary: A comprehensive proteomic analysis of 41 adult mouse tissues reveals evidence for protein products from at least 80% of all mouse genes. Comparison of tissue profiles between mouse and human suggests that the fundamental biology of this important model organism is even more different from our own than previously thought.
Article
Genetics & Heredity
Bangrong Cao, Liping Zhang, Huifen Liu, Shiqi Ma, Kun Mi
Summary: The study found that ACE2 and TMPRSS2 receptors are highly expressed in neonates, indicating higher susceptibility to infection. There is a high correlation between the expression of receptors and proteases during lung development, with TMPRSS9 being highly expressed in neonates. Additionally, immune cell infiltration analysis revealed immaturity in neonates, suggesting an association with mild or moderate cases of COVID-19.
FRONTIERS IN GENETICS
(2021)
Article
Multidisciplinary Sciences
Joey A. Charbonneau, Lara Maister, Manos Tsakiris, Eliza Bliss-Moreau
Summary: The ability to perceive internal bodily signals plays a critical role in biological and psychological functions. Rhesus monkeys have a human-like capacity to integrate interoceptive signals from the heart with exteroceptive audiovisual information.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Genetics & Heredity
Min Zhu, Guangneng Liao, Xiaohong Liu, Xu Luo, Gen Chen, Lingyun Wang, Chengshi Wang, Wen Liu, Xin Zeng, Yanrong Lu, Guang Yang, Jingqiu Cheng, Younan Chen
Summary: The loss of beta cell mass and function in aged population is closely related to the prevalence of Type 2 diabetes. This study focuses on the causal relations between aging and age-related pancreatic islets degeneration using rhesus monkeys as an animal model. The findings indicate that the decreased islet size, compromised insulin secretion, and loss of beta cell identity markers are key molecular changes in islet senescence.
Article
Biochemistry & Molecular Biology
Hilal A. Rather, Shalini Mishra, Yixin Su, Ashish Kumar, Sangeeta Singh, Biswapriya B. Misra, Jingyun Lee, Cristina M. Furdui, Lindsey R. Hamilton, Robert W. Gould, Susan H. Nader, Michael A. Nader, Gagan Deep
Summary: This study investigates the effects of prenatal cocaine use on postnatal development and long-term neurobiological outcomes. The researchers found that extracellular vesicles (EVs) could potentially serve as biomarkers to assess the adverse effects due to prenatal cocaine exposure.
Article
Veterinary Sciences
Christophe Casteleyn, Nina Robin, Jaco Bakker
Summary: This manuscript provides detailed anatomical data on the pelvic limb of the rhesus monkey, including the muscular, circulatory, and peripheral nerve systems. The study aims to improve the understanding of the anatomy of this widely used model in biomedical research, as well as enhance the welfare of captive individuals.
VETERINARY SCIENCES
(2023)
Article
Veterinary Sciences
Christophe Casteleyn, Charlotte Gram, Jaco Bakker
Summary: This manuscript provides detailed anatomical data on the rhesus monkey thoracic limb, fulfilling the needs of researchers and veterinarians. The anatomical structures are described and illustrated using colored images. The study identifies both similarities and differences between the anatomy of rhesus monkeys and humans, emphasizing the importance of understanding the anatomy of this primate species.
VETERINARY SCIENCES
(2023)
Article
Multidisciplinary Sciences
Munehisa Shimamura, Koichi Kaikita, Hironori Nakagami, Tomohiro Kawano, Nan Ju, Hiroki Hayashi, Ryo Nakamaru, Shota Yoshida, Tsutomu Sasaki, Hideki Mochizuki, Kenichi Tsujita, Ryuichi Morishita
Summary: Researchers redesigned an S100A9 vaccine that successfully produced antibodies and showed anti-thrombotic effects in cynomolgus monkeys. The vaccine was able to neutralize the anti-thrombotic effects of the produced antibodies and did not prolong bleeding time in vaccinated monkeys. Further studies are needed to enhance the vaccine's effectiveness and safety, but it may offer a promising approach to improving adherence to antiplatelet drugs in clinical settings.
SCIENTIFIC REPORTS
(2021)
Article
Clinical Neurology
Danielle Beckman, Paramita Chakrabarty, Sean Ott, Amanda Dao, Eric Zhou, William G. Janssen, Kristine Donis-Cox, Scott Muller, Jeffrey H. Kordower, John H. Morrison
Summary: This study successfully simulated tau protein misfolding and propagation similar to humans in rhesus monkeys, along with observing biomarkers of neuroinflammation and neuronal loss. These findings provide hope for the development of new therapies for AD.
ALZHEIMERS & DEMENTIA
(2021)
Article
Microbiology
Yaping Yan, Junfeng Wang, Shuai Qiu, Yanchao Duan, Wei Si
Summary: This study analyzed the intestinal microbiota composition of healthy rhesus monkeys in 6 GIT regions, providing important reference data for better understanding the regional organization and functions of gut microbial communities in humans.
MICROBIOLOGY SPECTRUM
(2022)
Article
Neurosciences
Kathleen A. Grant, Natali Newman, Colton Lynn, Conor Davenport, Steven Gonzales, Verginia C. Cuzon Carlson, Christopher D. Kroenke
Summary: The predisposition to engage in autonomous habitual behaviors is associated with behavioral disorders, and this study uses resting-state fMRI to identify primate brain networks that mediate trait-like deficits in ASST performance.
JOURNAL OF NEUROSCIENCE
(2022)
Editorial Material
Cell & Tissue Engineering
Masahito Yoshihara, Magdalena Wagner, Anastasios Damdimopoulos, Cheng Zhao, Sophie Petropoulos, Shintaro Katayama, Juha Kere, Fredrik Lanner, Pauliina Damdimopoulou
Summary: Ovaries play a crucial role in the development, fertility, and reproduction of women. They age faster than other tissues and their function is lost before other organs undergo senescence. While there have been debates about the presence of germline stem cells and neo-oogenesis in adult human ovaries, our recent analysis did not find evidence of such stem cells. However, we welcome discussions on different protocols and acknowledge the importance of excellence in methods.
Article
Neurosciences
Yue Cui, Haibin Huang, Jinquan Gao, Tianzi Jiang, Chen Zhang, Shan Yu
Summary: Hematological and biochemical blood traits are linked to brain structural characteristics in macaques, forming interactive networks. Different subnetworks of the brain are correlated with specific blood indices, revealing previously unknown relationships in brain structural organization.
Article
Neurosciences
Sophie Brule, Bastien Herlin, Pierre Pouget, Marcus Missal
Summary: After ketamine injection, temporal expectation significantly decreased in both subjects and movement latency increased. These effects are independent, indicating that ketamine may alter temporal expectation and lead to cognitive deficits.
PSYCHOPHARMACOLOGY
(2021)
Article
Neurosciences
Wei Qin, Qi Gan, Lei Yang, Yongchao Wang, Weizhi Qi, Bowen Ke, Lei Xi
Summary: The study utilized photoacoustic microscopy to investigate the structural and functional changes of cerebral vasculature in rhesus monkeys, revealing both vasodilatation and vasoconstriction under hypoxic conditions. The results suggest that photoacoustic microscopy is a promising method for studying neurovascular coupling and cerebral vascular diseases.
Article
Cell Biology
Meijie Qi, Haifeng Sun, Yueshuai Guo, Yu Zhou, Xueying Gu, Jiachuan Jin, Xiaoxu Chen, Fangzhu Wang, Honghui Ma, Xuejiang Guo, Hao Chen, Bin Shen
Summary: The study demonstrated the critical role of YTHDF2 in spermatogenesis by showing that YTHDF2 knockout led to male infertility and affected sperm morphology, motility, and fertilization ability. YTHDF2 facilitates timely turnover of stage-specific transcripts to ensure proper progression of spermatogenesis.
CELL PROLIFERATION
(2022)
Article
Biochemistry & Molecular Biology
Hui Zhou, Cong Shen, Yueshuai Guo, Xiaoyan Huang, Bo Zheng, Yibo Wu
Summary: This study reveals the interaction between plasminogen receptor and B lymphoma Mo-MLV insertion region 1, and its mechanism in maintaining spermatogonial stem cells by modulating oxidative stress and p16/p19 signaling.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Jianlong Ma, Yayun Gu, Juanjuan Liu, Jingmei Song, Tao Zhou, Min Jiang, Yang Wen, Xuejiang Guo, Zuomin Zhou, Jiahao Sha, Jianbo He, Zhibin Hu, Lingfei Luo, Mingxi Liu
Summary: Our previous study identified 53 SNPs associated with CHD in the Han Chinese population. In this study, we conducted functional screening of 27 orthologous genes in zebrafish and identified 5 genes essential for heart development. These novel CHD-related genes have presumptive roles in heart chamber and atrioventricular canal formation. Furthermore, we demonstrated that maml3 is required for Notch signaling in vivo, leading to defective cardiac trabeculation and heart failure in zebrafish embryos.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Biochemical Research Methods
Hongzheng Sun, Guangyi Sun, Haotian Zhang, Huiqing An, Yueshuai Guo, Juan Ge, Longsen Han, Shuai Zhu, Shoubin Tang, Congyang Li, Chen Xu, Xuejiang Guo, Qiang Wang
Summary: In this study, the temporal proteomic profiles of mouse oocytes during in vivo maturation were obtained, revealing novel proteomic features and key factors controlling histone acetylation state and meiotic cell cycle in oocytes. A broad resource on the dynamics occurring in oocyte proteome was provided, contributing to a better understanding of the molecular mechanisms during germ cell development.
MOLECULAR & CELLULAR PROTEOMICS
(2023)
Article
Multidisciplinary Sciences
Huiling Li, Yangao Huo, Xi He, Liping Yao, Hao Zhang, Yiqiang Cui, Huijuan Xiao, Wenxiu Xie, Dejiu Zhang, Yue Wang, Shu Zhang, Haixia Tu, Yiwei Cheng, Yueshuai Guo, Xintao Cao, Yunfei Zhu, Tao Jiang, Xuejiang Guo, Yan Qin, Jiahao Sha
Summary: A sperm-specific ribosome with a specialized nascent polypeptide exit tunnel has been identified in mice, which plays a crucial role in regulating protein folding during sperm formation.
Article
Biochemistry & Molecular Biology
Cheng Wang, Yayun Gu, Jun Zhou, Jie Zang, Xiufeng Ling, Hong Li, Lingmin Hu, Bei Xu, Bo Zhang, Na Qin, Hong Lv, Weiwei Duan, Yue Jiang, Yuanlin He, Tao Jiang, Congcong Chen, Xiumei Han, Kun Zhou, Bo Xu, Xiaoyu Liu, Shiyao Tao, Yangqian Jiang, Jiangbo Du, Juncheng Dai, Feiyang Diao, Chuncheng Lu, Xuejiang Guo, Ran Huo, Jiayin Liu, Yuan Lin, Yankai Xia, Guangfu Jin, Hongxia Ma, Hongbing Shen, Zhibin Hu
Summary: This study investigates the association between assisted reproductive technology (ART) and initial leukocyte telomere length (LTL) using whole-genome sequencing (WGS) data. The study finds that one-year-old children conceived by ART have shorter LTLs, especially in those conceived through blastocyst-stage embryo transfer. Mouse experiments also confirm that blastocyst-stage embryo transfer results in shorter telomere lengths.
Article
Biochemical Research Methods
Ping Zhang, Min Wang, Tao Zhou, Daozhen Chen
Summary: SeqWiz is a state-of-the-art toolkit for constructing next-generation sequence databases and performing proteomic-centric sequence analyses. It introduces two derived data formats, SQPD and SET, which outperform traditional FASTA or PEFF formats in terms of time and resource consumption. SeqWiz also provides compatible functions for handling traditional text formats and can promote the implementation of complementary proteomics for precision proteomics.
BMC BIOINFORMATICS
(2023)
Article
Biology
Yu Chen, Xiangzheng Zhang, Jiayin Jiang, Mengjiao Luo, Haixia Tu, Chen Xu, Huanhuan Tan, Xin Zhou, Hong Chen, Xudong Han, Qiuling Yue, Yueshuai Guo, Ke Zheng, Yaling Qi, Chenghao Situ, Yiqiang Cui, Xuejiang Guo
Summary: A study identifies a protein called Tssa that interacts with Miwi and is essential for male fertility, as it stabilizes mRNAs related to sperm formation.
Article
Biology
Shu Zhang, Chang Wang, Yue Wang, Hao Zhang, Chen Xu, Yiwei Cheng, Yan Yuan, Jiahao Sha, Xuejiang Guo, Yiqiang Cui
Summary: In this study, an endogenous circular RNA (circRsrc1) was discovered, which encodes a novel protein (Rsrc1-161aa). Deletion of Rsrc1-161aa in mice resulted in impaired male fertility, characterized by decreased sperm count and motility due to mitochondrial energy metabolism dysfunctions. In vitro experiments showed that circRsrc1 regulates mitochondrial functions through its encoded protein Rsrc1-161aa. Mechanistically, Rsrc1-161aa interacts with mitochondrial protein C1qbp to enhance its binding activity to mitochondrial mRNAs, thereby regulating mitochondrial ribosome assembly and translation.
Article
Biochemistry & Molecular Biology
Li Liu, Haojie Li, Mengjie Wang, Xiangzheng Zhang, Jie Ren, Yan Yuan, Jiahao Sha, Xuejiang Guo
Summary: Epigenetic regulation, specifically histone post-translational modifications (PTMs), is involved in the differentiation of spermatogonial stem cells (SSCs). However, due to the low number of SSCs in vivo, there is a lack of systematic studies on the regulation of histone PTMs during SSCs differentiation. In this study, we used targeted quantitative proteomics with mass spectrometry and RNA-seq data to quantify the dynamic changes of 46 different PTMs on histone H3.1 during SSCs differentiation in vitro. We identified seven differentially regulated histone H3.1 modifications and further identified specific binding proteins for H3K9me2 and H3S10ph, including transcription factors GTF2E2 and SUPT5H, which appear to play a crucial role in the epigenetic regulation of SSC differentiation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemical Research Methods
Bing Wang, Xiangzheng Zhang, Chen Xu, Xudong Han, Yue Wang, Chenghao Situ, Yan Li, Xuejiang Guo
Summary: The study introduces a new deep learning framework, DeepSP, for protein subcellular localization (PSL) prediction using mass spectrometry-based spatial proteomics data. By capturing detailed changes in protein occupancy profiles across subcellular fractions, DeepSP constructs a new feature map and utilizes a convolutional block attention module to enhance PSL prediction performance. DeepSP demonstrates significant improvement in accuracy and robustness compared to current machine learning predictors, making it a valuable tool for spatial proteomics studies and the understanding of protein function.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Biology
Tianyu Zhu, Yuxin Zhang, Xunlun Sheng, Xiangzheng Zhang, Yu Chen, Hongjing Zhu, Yueshuai Guo, Yaling Qi, Yichen Zhao, Qi Zhou, Xue Chen, Xuejiang Guo, Chen Zhao
Summary: We report CEP78 as a causative gene of a particular syndrome including cone-rod dystrophy (CRD) and male infertility with multiple morphological abnormalities of sperm flagella (MMAF) in human and mouse. Cep78 knockout mice exhibited impaired photoreceptor function and morphology, as well as male infertility and MMAF. CEP78 regulates the interaction, stability, and centriolar localization of its interacting protein, and insufficiency of CEP78 or its interacting protein results in abnormal centriole elongation and cilia shortening. Our study supports the important roles of CEP78 defects in centriole and ciliary dysfunctions and molecular pathogenesis of such multi-system syndrome.
Article
Cell Biology
Bo Yang, Chao Liu, Xiaohui Ju, Bingbing Wu, Zhuangfei Wang, Fucheng Dong, Yanying Yu, Xiaohui Hou, Min Fang, Fei Gao, Xuejiang Guo, Yaoting Gui, Qiang Ding, Wei Li
Summary: This study generated a mouse model expressing human ACE2 and SARS-CoV-2 N gene, allowing for the study of SARS-CoV-2 pathogenesis in specific tissues or organs. The model supports infection by different SARS-CoV-2 variants and can be used to evaluate the effectiveness of antiviral drugs.
Article
Biology
Xiaoxu Chen, Mingyue Chen, Yuqing Zhu, Haifeng Sun, Yue Wang, Yuan Xie, Lianfu Ji, Cheng Wang, Zhibin Hu, Xuejiang Guo, Zhengfeng Xu, Jun Zhang, Shiwei Yang, Dong Liang, Bin Shen
Summary: A proof-of-concept study demonstrates the potential of using a screened DdCBE pair to correct homoplasmic mitochondrial mutations and restore mitochondrial function in induced pluripotent stem cells.
COMMUNICATIONS BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Xudong Han, Bing Wang, Chenghao Situ, Yaling Qi, Hui Zhu, Yan Li, Xuejiang Guo
Summary: This study presents scapGNN, a single-cell data analysis toolkit that utilizes a graph neural network (GNN) to transform sparse single-cell profile data into a stable gene-cell association network. It accurately infers single-cell pathway activity scores and identifies cell phenotype-associated gene modules from single-cell multi-omics data. The systematic benchmarking results demonstrate superior performance of scapGNN compared to state-of-the-art methods in various downstream single-cell analyses.
