Minimum set of mutations needed to optimize cyan fluorescent proteins for live cell imaging

Structure of a fluorescent protein fromAequorea victoriabearing the obligate-monomer mutation A206K

(2012) David von Stetten et al.   Acta Crystallographica Section F-Structural Biology and Crystallization Communications

The Single T65S Mutation Generates Brighter Cyan Fluorescent Proteins with Increased Photostability and pH Insensitivity

(2012) Asma Fredj et al.   PLoS One

Structure-guided evolution of cyan fluorescent proteins towards a quantum yield of 93%

(2012) Joachim Goedhart et al.   Nature Communications

Spatiotemporal Regulation of Small GTPases as Revealed by Probes Based on the Principle of Förster Resonance Energy Transfer (FRET): Implications for Signaling and Pharmacology

(2011) Etsuko Kiyokawa et al.   Annual Review of Pharmacology and Toxicology

Precise measurement of protein interacting fractions with fluorescence lifetime imaging microscopy

(2011) Kirstin A. Walther et al.   Molecular BioSystems

pH-sensitivity of YFP provides an intracellular indicator of programmed cell death

(2011) Bennett Young et al.   Plant Methods

An Improved Cerulean Fluorescent Protein with Enhanced Brightness and Reduced Reversible Photoswitching

(2011) Michele L. Markwardt et al.   PLoS One

Cyan Fluorescent Protein Carries a Constitutive Mutation That Prevents Its Dimerization

(2010) Agathe Espagne et al.   BIOCHEMISTRY

Type 4 Phosphodiesterase Plays Different Integrating Roles in Different Cellular Domains in Pyramidal Cortical Neurons

(2010) L. R. V. Castro et al.   JOURNAL OF NEUROSCIENCE

Bright cyan fluorescent protein variants identified by fluorescence lifetime screening

(2010) Joachim Goedhart et al.   NATURE METHODS

Fluorescent Proteins and Their Applications in Imaging Living Cells and Tissues

(2010) Dmitriy M. Chudakov et al.   PHYSIOLOGICAL REVIEWS

ATP Changes the Fluorescence Lifetime of Cyan Fluorescent Protein via an Interaction with His148

(2010) Jan Willem Borst et al.   PLoS One

Intrinsic Dynamics in ECFP and Cerulean Control Fluorescence Quantum Yield

(2009) Mickaël Lelimousin et al.   BIOCHEMISTRY

Probing Nuclear Localization Signal-Importin α Binding Equilibria in Living Cells

(2009) Francesco Cardarelli et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Are the Fluorescent Properties of the Cyan Fluorescent Protein Sensitive to Conditions of Oxidative Stress?

(2009) Luis Alvarez et al.   PHOTOCHEMISTRY AND PHOTOBIOLOGY

Relation between pH, structure, and absorption spectrum of Cerulean: A study by molecular dynamics and TD DFT calculations

(2009) Germain Vallverdu et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

Complex Fluorescence of the Cyan Fluorescent Protein: Comparisons with the H148D Variant and Consequences for Quantitative Cell Imaging†

(2008) Aude Villoing et al.   BIOCHEMISTRY

pHlameleons: A Family of FRET-Based Protein Sensors for Quantitative pH Imaging†

(2008) Alessandro Esposito et al.   BIOCHEMISTRY

Improving the photostability of bright monomeric orange and red fluorescent proteins

(2008) Nathan C Shaner et al.   NATURE METHODS