Article
Biochemistry & Molecular Biology
Reena Ghildyal, Michael N. Teng, Kim C. Tran, John Mills, Marco G. Casarotto, Philip G. Bardin, David A. Jans
Summary: Respiratory syncytial virus (RSV) is a major cause of respiratory infections in infants and the elderly. The study identified protein kinase CK2 as a regulator of RSV matrix (M) protein's distribution between the nucleus and cytoplasm. Serine (S) 95 and threonine (T) 205 were found to be key CK2 sites. These findings provide insights for developing antiviral strategies targeting CK2.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Pharmacology & Pharmacy
Shuang Cui, Huijian Hu, An Chen, Ming Cui, Xiaojie Pan, Pengfei Zhang, Guangji Wang, Hong Wang, Haiping Hao
Summary: The acetylation of FXR regulates its nucleocytoplasmic shuttling and degradation under conditions of liver injury, which limits the clinical benefits of FXR agonists. SIRT1 activators can reduce FXR acetylation and prevent its cytosolic degradation, and combining SIRT1 activators with FXR agonists shows promise in treating liver diseases.
ACTA PHARMACEUTICA SINICA B
(2023)
Article
Cell Biology
Yongsheng Huang, Sijia Liu, Mengjie Shan, Sophie C. Hagenaars, Wilma E. Mesker, Danielle Cohen, Lin Wang, Zhi Zheng, Peter Devilee, Rob A. E. M. Tollenaar, Zhangfu Li, Yongmei Song, Long Zhang, Dan Li, Peter Ten Dijke
Summary: RNF12, overexpressed in invasive breast cancers, promotes migration and invasion of breast cancer cells through its interaction with the AKT signaling pathway. Phosphorylation of AKT induces nuclear localization of RNF12 and accelerates the degradation of SMAD7 mediated by RNF12. Moreover, RNF12 expression is positively correlated with phosphorylated AKT and phosphorylated SMAD2 levels.
CELL DEATH & DISEASE
(2022)
Article
Endocrinology & Metabolism
Fabiola Garcia Cortizo, Daniel Pfaff, Angela Wirth, Andrea Schlotterer, Rebekka Medert, Jakob Morgenstern, Tobias Weber, Hans-Peter Hammes, Thomas Fleming, Peter Paul Nawroth, Marc Freichel, Aurelio A. Teleman
Summary: The study demonstrates that post-translational modifications, specifically the phosphorylation of Glo1 at Tyrosine 136, can enhance Glo1 activity for detoxifying MG. However, high glucose levels lead to decreased Glo1 activity, resulting in elevated MG levels and potentially initiating a detrimental positive feedback loop contributing to diabetic complications.
MOLECULAR METABOLISM
(2022)
Article
Cell Biology
Eva-Maria Blumrich, Jessica C. Nicholson-Fish, Marie Pronot, Elizabeth C. Davenport, Dominic Kurian, Adam Cole, Karen J. Smillie, Michael A. Cousin
Summary: Phosphatidylinositol 4-kinase IIa (PI4KIIa) is essential for activity-dependent bulk endocytosis (ADBE) in primary neuronal cultures. The phosphorylation of Ser-47 by glycogen synthase kinase 3(3 (GSK3(3) is crucial for ADBE. Phosphomimetic PI4KIIa interacts with specific presynaptic molecules, including AGAP2 and CAMKV, which are also essential for ADBE when depleted in neurons.
Article
Immunology
Feifei Chen, Hongxia Di, Yanhui Wang, Chao Peng, Rongrong Chen, Huiwen Pan, Cai-Guang Yang, Haihua Liang, Lefu Lan
Summary: Phosphorylation plays an important role in modulating the activity of the transpeptidase enzyme SrtA in Staphylococcus aureus. The catalytic site residue cysteine-184 of SrtA is primarily phosphorylated by Stk1 kinase or small-molecule phosphodonor AcP. Deletion of the phosphatase gene Stp1 increases the phosphorylation level of SrtA and leads to phenotypic defects.
Article
Biology
Francisco Bustos, Sunil Mathur, Carmen Espejo-Serrano, Rachel Toth, C. James Hastie, Satpal Virdee, Greg M. Findlay
Summary: This study utilized photocrosslinking activity-based probes to monitor the RING E3 ubiquitin ligase activity of RNF12 and demonstrated its application in assessing the impact of RNF12 variants on activity. The findings showed that the photoABPs accurately reported the effects of RNF12 variants on E3 activity, and the technology could be rapidly deployed in human pluripotent stem cells.
LIFE SCIENCE ALLIANCE
(2022)
Article
Biochemistry & Molecular Biology
Junwon Kim, Mark D. Rose
Summary: In Saccharomyces cerevisiae, the p21-activated kinase Cla4p regulates polarized morphogenesis and cytokinesis. Cla4p kinase activity is down-regulated during mating, and this is achieved through phosphorylation of Cla4p at multiple sites, including Cla4p-S186, which reduces its kinase activity. Fus3p counteracts Cla4p kinase activity during mating by phosphorylating Fus2p at different sites to promote nuclear export and block import.
Article
Biotechnology & Applied Microbiology
Zahra Safaei, Gary L. L. Thompson
Summary: This study demonstrates the impact of microsecond pulsed electric fields on nucleocytoplasmic transport of HDAC4 and HDAC5, which is dependent on the presence of extracellular calcium ions. By inducing an increase in intracellular Ca2+ concentration, a signaling pathway is activated, leading to the nuclear export and import of HDAC4 and HDAC5. This research opens up new opportunities in the field of epigenetic modification.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Godfrey Dzhivhuho, Jordan Holsey, Ethan Honeycutt, Heather O'Farrell, David Rekosh, Marie-Louise Hammarskjold, Patrick E. H. Jackson
Summary: During HIV infection, the transport of viral mRNA from the cell nucleus to the cytoplasm is facilitated by Rev protein and the Rev Response Element (RRE). The activity of Rev protein, which varies among different primary isolates, is determined by amino acid differences within the oligomerization domain. Small sequence changes can modulate Rev-RRE activity, potentially impacting HIV pathogenesis.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Alicyia Walczyk-Mooradally, Jennifer Holborn, Karamjeet Singh, Marshall Tyler, Debasis Patnaik, Hendrik Wesseling, Nicholas J. Brandon, Judith Steen, Steffen P. Graether, Stephen J. Haggarty, Jasmin Lalonde
Summary: Arc is a protein crucial for cognitive function and memory formation, able to self-assemble into virus-like capsids for mRNA transportation. The study shows that TNIK kinase can modify Arc at Serine 67 and Threonine 278, influencing its subcellular distribution and self-assembly. Understanding the interaction between Arc and TNIK in neuronal cells could offer new insights into psychiatric disorders.
JOURNAL OF NEUROCHEMISTRY
(2021)
Article
Cardiac & Cardiovascular Systems
Galen T. Squiers, Micheal A. McLellan, Alexei Ilinykh, Jane Branca, Nadia A. Rosenthal, Alexander R. Pinto
Summary: This study reveals that the cellular composition of mouse heart is hormone-dependent, with distinct landscapes in females and males that are plastic and can be rapidly modulated by endocrine factors. These findings have implications for therapeutic strategies targeting cardiac cellular heterogeneity and highlight the importance of biological sex in studies of cardiac physiology and stress responses.
CARDIOVASCULAR RESEARCH
(2021)
Article
Multidisciplinary Sciences
Nikola Arsic, Tania Slatter, Gilles Gadea, Etienne Villain, Aurelie Fournet, Marina Kazantseva, Frederic Allemand, Nathalie Sibille, Martial Seveno, Sylvain de Rossi, Sunali Mehta, Serge Urbach, Jean-Christophe Bourdon, Pau Bernado, Andrey Kajava, Antony Braithwaite, Pierre Roux
Summary: The p53 isoform Delta 133p53 beta promotes intrinsic oncogenic functions, with its activity regulated through an aggregation-dependent mechanism. Interaction with partners like p63 family members or the CCT chaperone complex influences cancer cell features such as migration and invasion by modulating Delta 133p53 beta activity.
NATURE COMMUNICATIONS
(2021)
Article
Biology
Charlene Estrada, Liliana Mirabal-Ortega, Laurence Mery, Florent Dingli, Laetitia Besse, Cedric Messaoudi, Damarys Loew, Celio Pouponnot, Corine Bertolotto, Alain Eychene, Sabine Druillennec
Summary: The MITF transcription factor directly interacts with RAF kinases, including ARAF, BRAF and CRAF, in melanoma cells. This interaction negatively regulates MITF activity by promoting its accumulation in the cytoplasm.
COMMUNICATIONS BIOLOGY
(2022)
Article
Cell Biology
Michelle L. Swift, Kate Beishline, Samuel Flashner, Jane Azizkhan-Clifford
Summary: This passage discusses the role of Sp1 in the cell cycle and its regulation of DSB repair pathway choice, favoring NHEJ repair. Sp1 is shown to recruit the NHEJ repair factor 53BP1 in G1 phase, while being evicted through phosphorylation in S phase, inhibiting HR repair.
Article
Biotechnology & Applied Microbiology
Su Jung Oh-Hohenhorst, Derya Tilki, Ann-Kristin Ahlers, Anna Suling, Oliver Hahn, Pierre Tennstedt, Christiane Matuszcak, Hanna Maar, Vera Labitzky, Sandra Hanika, Sarah Starzonek, Simon Baumgart, Steven A. Johnsen, Martina Kluth, Hueseyin Sirma, Ronald Simon, Guido Sauter, Hartwig Huland, Udo Schumacher, Tobias Lange
Summary: The deletion of the tumor suppressor gene CHD1 has been found to increase the risk of postoperative metastasis and promote spontaneous metastasis formation in prostate cancer patients after R0 resection. Additionally, CHD1 loss affects intratumoral recurrence and cancer-specific survival time in these patients.
CANCER GENE THERAPY
(2022)
Article
Oncology
Robyn Laura Kosinsky, Dominik Saul, Christoph Ammer-Herrmenau, William A. Faubion, Albrecht Neesse, Steven A. Johnsen
Summary: The study identified a tumor-suppressive function of USP22 in colorectal cancer (CRC) and its impact on intestinal inflammation. Loss of Usp22 resulted in more severe colitis and increased inflammation-associated tumor growth. Depletion of USP22 induced upregulation of SPARC, indicating its role in regulating inflammation in colitis and CRC.
Review
Cell Biology
Ana P. Kutschat, Steven A. Johnsen, Feda H. Hamdan
Summary: PDAC has a poor prognosis and low survival rate due to late diagnosis and chemotherapy resistance. Genomic aberrations and epigenomic changes shift cellular signaling response in pancreatic tumors. Genetic amplifications can reroute calcium signaling and confer malignant attributes to tumors.
Article
Oncology
Xiyin Li, Hairui Wang, Xing Yang, Xiaoqi Wang, Lina Zhao, Li Zou, Qin Yang, Zongliu Hou, Jing Tan, Honglei Zhang, Jianyun Nie, Baowei Jiao
Summary: Through research, it was found that GABRP can sustain the stemness of TNBC by modulating EGFR expression, affecting sensitivity to chemotherapy drugs, indicating that GABRP may be a potential therapeutic target.
Article
Biochemistry & Molecular Biology
Robyn Laura Kosinsky, Maria Zerche, Ana Patricia Kutschat, Asha Nair, Zhenqing Ye, Dominik Saul, Maximilian von Heesen, Jessica J. Friton, Ana Carolina Schwarzer, Nadia Paglilla, Shehzad Z. Sheikh, Florian Wegwitz, Zhifu Sun, Michael Ghadimi, Rodney D. Newberry, R. Balfour Sartor, William A. Faubion, Steven A. Johnsen
Summary: Despite the identification of genetic factors linked to IBD susceptibility, the mechanisms behind it are still unclear. The RNF20/RNF40/H2Bub1 axis plays a critical role in promoting intestinal inflammation in IBD patients and mouse models, revealing their importance as regulators of IBD.
CELL DEATH AND DIFFERENTIATION
(2021)
Meeting Abstract
Oncology
Cody Nesvick, Liang Zhang, Alex Wixom, Feda Hamdan, Steven Johnsen, David Daniels
Article
Multidisciplinary Sciences
Mengfei Liu, Sheng Cao, Li He, Jinhang Gao, Juan P. Arab, Huarui Cui, Weixia Xuan, Yandong Gao, Tejasav S. Sehrawat, Feda H. Hamdan, Meritxell Ventura-Cots, Josepmaria Argemi, William C. K. Pomerantz, Steven A. Johnsen, Jeong-Heon Lee, Fei Gao, Tamas Ordog, Philippe Mathurin, Alexander Revzin, Ramon Bataller, Huihuang Yan, Vijay H. Shah
Summary: Alcoholic hepatitis is characterized by intense liver inflammation driven by excessive cytokines and chemokines production and immune cell infiltration. The study identified a super-enhancer that regulates the expression of multiple CXCL chemokines in alcoholic hepatitis and may be a potential therapeutic target.
NATURE COMMUNICATIONS
(2021)
Article
Cell Biology
Palak Ahuja, Chun Fai Ng, Brian Pak Shing Pang, Wing Suen Chan, Margaret Chui Ling Tse, Xinyi Bi, Hiu-Lam Rachel Kwan, Daniel Brobst, Oana Herlea-Pana, Xiuying Yang, Guanhua Du, Suchaorn Saengnipanthkul, Hye Lim Noh, Baowei Jiao, Jason K. Kim, Chi Wai Lee, Keqiang Ye, Chi Bun Chan
Summary: The study reveals that BDNF promotes mitochondrial fission and clearance in skeletal muscle, maintaining mitochondrial quality and function. Muscle-specific bdnf knockout mice fed with a high-fat diet exhibit mitochondrial defects and metabolic disorders.
Article
Oncology
Kirsten G. M. Aspros, Jodi M. Carter, Tanya L. Hoskin, Vera J. Suman, Malayannan Subramaniam, Michael J. Emch, Zhenqing Ye, Zhifu Sun, Jason P. Sinnwell, Kevin J. Thompson, Xiaojia Tang, Esther P. B. Rodman, Xiyin Wang, Adam W. Nelson, Igor Chernukhin, Feda H. Hamdan, Elizabeth S. Bruinsma, Jason S. Carroll, Martin E. Fernandez-Zapico, Steven A. Johnsen, Krishna R. Kalari, Haojie Huang, Roberto A. Leon-Ferre, Fergus J. Couch, James N. Ingle, Matthew P. Goetz, John R. Hawse
Summary: This study reported the expression profiles of ER beta in the largest cohort of TNBC to date and its association with clinicopathological features and patient outcomes. ER beta was found to be expressed in approximately 18% of TNBCs and its expression was associated with favorable clinicopathological features. Mechanistically, ER beta formed a co-repressor complex to suppress oncogenic NF kappa B/RELA (p65) activity.
Article
Oncology
Hui Wang, Haibo Xu, Wei Chen, Mei Cheng, Li Zou, Qin Yang, Chi Bun Chan, Hao Zhu, Ceshi Chen, Jianyun Nie, Baowei Jiao
Summary: The study reveals that Rab13 is highly expressed in breast CSCs and its depletion can suppress breast cancer cell stemness, tumorigenesis, and chemoresistance by controlling the membrane translocation of CXCR1/2, establishing a supportive BCSC niche. Targeting the Rab13-mediated BCSC niche with bardoxolone-methyl prevents BCSC stemness in vitro and in vivo, providing a potential therapeutic strategy for disrupting the BCSC niche.
Article
Gastroenterology & Hepatology
Palak Ahuja, Xinyi Bi, Chun Fai Ng, Margaret Chui Ling Tse, Miaojia Hang, Brian Pak Shing Pang, Elsie Chit Yu Iu, Wing Suen Chan, Xin Ci Ooi, Anqi Sun, Oana Herlea-Pana, Zhixue Liu, Xiuying Yang, Baowei Jiao, Xin Ma, Kelvin Ka Lok Wu, Leo Tsz On Lee, Kenneth King Yip Cheng, Chi Wai Lee, Chi Bun Chan
Summary: This study found that SBK1 is a regulator of hepatic lipid metabolism and systemic insulin sensitivity in response to obesity through the Nur77-FGF21 pathway.
Article
Cell Biology
Lu Guo, Hao Ke, Honglei Zhang, Li Zou, Qin Yang, Xuemei Lu, Limin Zhao, Baowei Jiao
Summary: Alternative splicing (AS) plays a crucial role in the progression of triple-negative breast cancer (TNBC), especially in maintaining the stemness of breast cancer stem cells (BCSCs). The splicing regulator TAR DNA-binding protein-43 (TDP43) and its partner serine-arginine-rich splicing factor 3 (SRSF3) are identified as important factors in regulating CD44 variant AS isoforms (CD44v) and BCSC stemness. Mechanistically, SRSF3 stabilizes TDP43 mRNA by inhibiting nonsense-mediated decay (NMD), thereby maintaining the abundance of CD44v.
CELL DEATH & DISEASE
(2022)
Meeting Abstract
Neurosciences
H. M. Wuest, A. Wegener, F. Froeb, A. C. Hartwig, F. Wegwitz, V. L. Kari, M. Schimmel, E. R. Tamm, S. A. Johnsen, M. Wegner, E. Sock
Article
Medicine, Research & Experimental
Michelle M. Gonzalez, Adebowale O. Bamidele, Phyllis A. Svingen, Mary R. Sagstetter, Thomas C. Smyrk, Joseph M. Gaballa, Feda H. Hamdan, Robyn Laura Kosinsky, Hunter R. Gibbons, Zhifu Sun, Zhenqing Ye, Asha Nair, Guilherme P. Ramos, Manuel B. Braga Neto, Alexander Q. Wixom, Angela J. Mathison, Steven A. Johnsen, Raul Urrutia, William A. Faubion
Summary: Research has shown that BMI1 regulates the inflammatory enhancer network in Treg cells, and its loss leads to systemic inflammation and phenotypic conversion of Treg cells, which is relevant to CD4(+) T cells associated with Crohn's disease.
JOURNAL OF CLINICAL INVESTIGATION
(2021)
Meeting Abstract
Gastroenterology & Hepatology
Guilherme Piovezani Ramos, Adebowale O. Bamidele, Mary R. Sagstetter, Emily Klatt, Robyn Laura Kosinsky, Feda H. Hamdan, Joseph Gaballa, Manuel B. Braga Neto, Ahmed T. Kurdi, Maria Westphal, Hunter R. Gibbons, Asha Nair, Andrew C. Osborne, Zhifu Sun, Steven A. Johnsen, Gwen Lomberk, William Faubion