Article
Multidisciplinary Sciences
Wei-Qi Lin, Zhen-Kai Ngian, Tong-Wey Koh, Chin-Tong Ong
Summary: This study found that reduced levels of nuclear lamin-B protein in Drosophila coincided with a decline in locomotor activity and stress resistance. Tissue-specific expression of lamin-B may regulate different aspects of animal physiology during aging.
Article
Chemistry, Multidisciplinary
Yizhuo Che, Xiaofei Yang, Peng Jia, Tingjie Wang, Dan Xu, Tianxue Guo, Kai Ye
Summary: This study introduces two global physical properties, DNA density and distance to nuclear periphery, and constructs a 2D matrix called D-2 plot for mapping genetic and epigenetic markers. Functional markers on the D-2 plot show distinct patterns, indicating its ability to compartmentalize functional genome regions. Furthermore, this study successfully identifies constantly active and newly activated genes during olfactory sensory neuron maturation based on the trajectories on the D-2 plot. The research reveals that the D-2 plot effectively categorizes functional regions and provides a universal and transcription-related measurement for the 3D genome.
Review
Biochemistry & Molecular Biology
Maria Moriel-Carretero
Summary: This article highlights the lack of research on the role of lipids in genome homeostasis and how it discourages new initiatives. It proposes exploring the impact of lipids on genome homeostasis and suggests a comprehensive set of techniques for nucleus-centered researchers.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biology
Pau Pascual-Garcia, Shawn C. Little, Maya Capelson
Summary: Cells are able to develop transcriptional memory after repeated exposure to external cues, and this memory can be maintained epigenetically through cell divisions with the help of a nuclear pore component called Nup98. This study used single-molecule RNA FISH to examine the dynamics of transcription in Drosophila cells upon repeated exposure to the steroid hormone ecdysone. The results showed that cells rapidly activate a low-level transcriptional response upon hormone exposure, but also slowly transition into a specialized memory state characterized by a high rate of expression. It was found that this transition between non-memory and memory states is independent of the initial activation of transcription.
Article
Biochemistry & Molecular Biology
Moonjeong Choi, Hayeong Kwon, Kyuho Jeong, Yunbae Pak
Summary: This study reveals that Caveolin-2 plays a role as an epigenetic regulator in adipogenesis by controlling the activation of Cebpb.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2022)
Article
Plant Sciences
Gaurav Singh, Morgane Batzenschlager, Denisa Tomkova, Etienne Herzog, Elise Hoffmann, Guy Houlne, Anne-Catherine Schmit, Alexandre Berr, Marie-Edith Chaboute
Summary: This study investigates the role of inner nuclear membrane proteins and nuclear pore-associated components in maintaining genetic stability in plants. The gamma-tubulin complex component 3-interacting protein (gip1gip2) double mutants with defective nuclear shaping were used as a model. The results show that GIP1 and GIP2 play redundant roles in maintaining genome stability, with defects in these proteins leading to constitutive DNA damage and activation of the DNA damage response. Furthermore, the expression of GIP1-GFP in the mutants rescues the cellular response to DNA damage and promotes the colocalization of RAD51 and gamma-H2AX foci, suggesting the involvement of GIPs in the spatio-temporal recruitment of RAD51 at the nuclear periphery.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Multidisciplinary Sciences
Yuexia Wang, Khurts Shilagardi, Trunee Hsu, Kamsi O. Odinammadu, Takamitsu Maruyama, Wei Wu, Chyuan-Sheng Lin, Christopher B. Damoci, Eric D. Spear, Ji-Yeon Shin, Wei Hsu, Susan Michaelis, Howard J. Worman
Summary: Prelamin A, a farnesylated precursor of lamin A, can cause progeria syndrome when accumulated as progerin. Mutations in ZMPSTE24, the processing enzyme of prelamin A, can lead to progeroid disorders. This study generated a mouse model with permanently farnesylated prelamin A and found that these mice have extended lifespan and exhibit skeletal and nuclear defects during physiological aging.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Cell Biology
Jinmei Cheng, Edward S. Allgeyer, Jennifer H. Richens, Edo Dzafic, Amandine Palandri, Bohdan Lewkow, George Sirinakis, Daniel St Johnston
Summary: The line scanning microscope provides optical sectioning for single-molecule localization microscopy (SMLM) in tissues, achieving high-resolution imaging of nuclear pores and F-actin. The study reveals nonrandom distribution of nuclear pores in most Drosophila tissues, in contrast to what is observed in cultured cells. Lamin Dm(0) shows complementary localization to the nuclear pores, while ectopic expression of tissue-specific Lamin C leads to more random distribution of nuclear pores.
JOURNAL OF CELL SCIENCE
(2021)
Article
Biochemical Research Methods
Emily R. Cliff, Robin L. Kirkpatrick, Daniel Cunningham-Bryant, Brianna Fernandez, Joseph L. Harman, Jesse G. Zalatan
Summary: Research on the relationship between genome structure and function using a programmable CRISPR-Cas system in yeast showed that tethering the HMR locus did not significantly silence gene expression. A comparison with the Gal4 system revealed that the silencing effect is unexpectedly dependent on the properties of the protein tether. Additionally, the CRISPR-Cas system was unable to recruit the GAL2 locus to the nuclear periphery, suggesting potential challenges in synthetic genome structure perturbations.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Lisa Diez, Larisa E. Kapinos, Janine Hochmair, Sabrina Huebschmann, Alvaro Dominguez-Baquero, Amelie Vogt, Marija Rankovic, Markus Zweckstetter, Roderick Y. H. Lim, Susanne Wegmann
Summary: This study investigates the interactions between Tau and FG Nup, and how Tau phosphorylation and oligomerization affect these interactions. The results suggest that phosphorylation facilitates the accumulation of Tau with FG Nup, while oligomerization inhibits their interactions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Veer I. P. Keizer, Simon Grosse-Holz, Maxime Woringer, Laura Zambon, Koceila Aize, Maud Bongaerts, Fanny Delille, Lorena Kolar-Znika, Vittore F. Scolari, Sebastian Hoffmann, Edward J. Banigan, Leonid A. Mirny, Maxime Dahan, Daniele Fachinetti, Antoine Coulon
Summary: This study introduces a method to manipulate a genomic locus using controlled magnetic forces inside the nucleus of a living human cell. The results show that chromatin exhibits viscoelastic displacements in response to near-piconewton forces, challenging the view that interphase chromatin is a gel-like material.
Article
Multidisciplinary Sciences
Eszter E. Najbauer, Sheung Chun Ng, Christian Griesinger, Dirk Goerlich, Loren B. Andreas
Summary: The cohesive FG domains in nuclear pore complexes form a selective permeability barrier, with nanoscopic insight into their interactions hindered by sequence heterogeneity. By using an engineered repetitive sequence and NMR spectroscopy, researchers were able to overcome this challenge and map the dynamics of cohesive interactions. The study found that the FG repeats remained disordered in both phase-separated and soluble states, with slow translation observed in phase-separated domains.
NATURE COMMUNICATIONS
(2022)
Article
Cell Biology
Annael Brunet, Nicolas Destainville, Philippe Collas
Summary: The study utilized polymer simulations to investigate how physical parameters of chromatin affect its interactions with the nuclear lamina. It found that polymer stiffness has a greater impact on configurations at the lamina compared to stretching, leading to extended interactions and desorbed regions. The presence of an attraction potential results in persistent interaction and adsorption-desorption regimes, modulated by polymer stiffness and stretching.
Review
Cell Biology
Hyeong Jae Kim, Peter C. W. Lee, Jeong Hee Hong
Summary: With the discovery of the role of the nuclear envelope protein lamin in human genetic diseases, further diverse roles of lamins have been elucidated. The roles of lamins have been addressed in cellular homeostasis including gene regulation, cell cycle, cellular senescence, adipogenesis, bone remodeling as well as modulation of cancer biology. The modulatory roles of lamin-A/C in stem cell differentiation, skin, cardiac regulation, and oncology have also been elucidated.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Cell Biology
Eftekhar Eftekharpour
Summary: Aging leads to age-associated diseases, particularly affecting the nervous system. Alzheimer's disease is a well-known example, characterized by the accumulation of neurofibrillary tangles and amyloid plaques. Therapeutic attempts targeting these aggregates have been unsuccessful, highlighting the need to understand the underlying mechanisms. Recent research has shown the importance of neuronal nucleus damage, specifically nuclear lamina damage, in the pathophysiology of the disease. This research perspective emphasizes the significance of targeting the neuronal nucleus for potential therapeutic interventions.
Article
Developmental Biology
Catherine D. McCusker, Antony Athippozhy, Carlos Diaz-Castillo, Charless Fowlkes, David M. Gardiner, Randal Voss
BMC DEVELOPMENTAL BIOLOGY
(2015)
Article
Multidisciplinary Sciences
Carlos Diaz-Castillo
Article
Developmental Biology
Catherine D. McCusker, Carlos Diaz-Castillo, Julian Sosnik, Anne Q. Phan, David M. Gardiner
DEVELOPMENTAL BIOLOGY
(2016)
Article
Evolutionary Biology
Carlos Diaz-Castillo
EVOLUTIONARY BIOLOGY
(2017)
Article
Multidisciplinary Sciences
Raquel Chamorro-Garcia, Carlos Diaz-Castillo, Bassem M. Shoucri, Heidi Kach, Ron Leavitt, Toshi Shioda, Bruce Blumberg
NATURE COMMUNICATIONS
(2017)
Article
Multidisciplinary Sciences
Carlos Diaz-Castillo
Article
Evolutionary Biology
Carlos Diaz-Castillo
EVOLUTIONARY BIOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Carlos Diaz-Castillo, Jose M. Ranz
Article
Biochemistry & Molecular Biology
Carlos Diaz-Castillo, Jose M. Ranz
MOLECULAR BIOLOGY AND EVOLUTION
(2012)
Article
Multidisciplinary Sciences
Carlos Diaz-Castillo
Article
Genetics & Heredity
Carlos Diaz-Castillo, Xiao-Qin Xia, Jose M. Ranz
Article
Multidisciplinary Sciences
Carlos Diaz-Castillo, Raquel Chamorro-Garcia, Toshi Shioda, Bruce Blumberg
SCIENTIFIC REPORTS
(2019)
Review
Toxicology
Daniel D. Davis, Carlos Diaz-Castillo, Raquel Chamorro-Garcia
Summary: The role of environment in the development of diseases has long been known, but it is still unclear how different environmental factors interact to cause diseases. Metabolic disorders such as obesity and type 2 diabetes are associated with exposure to environmental chemicals during critical windows of susceptibility, such as the periconception, prenatal, and early life periods, and this effect can persist through development and across generations.
FRONTIERS IN TOXICOLOGY
(2022)
Article
Multidisciplinary Sciences
Catherine D. McCusker, Carlos Diaz-Castillo, Julian Sosnik, Anne Phan, David M. Gardiner