Article
Microbiology
Miao Zeng, Wei Zhang, Bowen Jiang, Tong Lu, Tao Hu, Mingshu Wang, Renyong Jia, Dekang Zhu, Mafeng Liu, Xinxin Zhao, Qiao Yang, Ying Wu, Shaqiu Zhang, Juan Huang, Xumin Ou, Sai Mao, Qun Gao, Di Sun, Ling Zhang, Anchun Cheng, Andres Merits, Shun Chen
Summary: By studying the role of Flavivirus nonstructural protein 5 (NS5) in TMUV replication and proliferation, it was found that the MTase-RdRp interface and the GTR linker play important roles in the affinity of NS5 protein. Further mutation analysis also provided insights into the replication ability and properties of TMUV.
VETERINARY MICROBIOLOGY
(2022)
Review
Microbiology
Emina Pustijanac, Moira Bursic, Jasminka Talapko, Ivana Skrlec, Tomislav Mestrovic, Dubravka Lisnjic
Summary: Tick-borne encephalitis virus (TBEV) is a flavivirus that can cause serious infection of the central nervous system in humans through tick bites. As there is no specific antiviral therapy available, treatment primarily focuses on symptomatic relief and support. However, active immunization is highly effective, particularly in endemic areas. The incidence of TBEV infections is increasing, posing a growing health concern.
Article
Chemistry, Medicinal
Weiyi Qian, Guang-Feng Zhou, Xintong Ge, Jian-Xia Xue, Chang-Bo Zheng, Liu-Meng Yang, Xue-Rong Gu, Chunyan Li, Si-Dong Xiong, Yong-Tang Zheng, Guo-Chun Zhou
Summary: In this study, potent anti-ZIKV activity was discovered in some dehydroandrographolide derivatives, with low cytotoxicity. Compound 5a and its analogues were found to inhibit ZIKV at the post-entry stage of the viral life cycle. Active compounds 3a, 5a, 5b, and 5c were shown to possess inhibitory activities against ZIKV NS5 MTase enzymatic activity. The study also revealed the importance of bulky groups at the C19 position for anti-ZIKV activity, and the superior effectiveness of the dehydroandrographolide backbone compared to andrographolide.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Education, Scientific Disciplines
Afaf S. Alwabli, Ishtiaq Qadri
Summary: This in-silico study identified potential lead molecules with drug-like properties to inhibit the activity of NS5 protein in Dengue virus infections. Natural compounds such as galactomannan and carrageenan were found to be top inhibitors of NS5 protein. Experimental validation of these lead molecules could lead to the development of therapeutics for viral infections like Dengue.
INDIAN JOURNAL OF PHARMACEUTICAL EDUCATION AND RESEARCH
(2021)
Review
Immunology
Jia Yi, Jiameng Miao, Qingwei Zuo, Felix Owusu, Qiutong Dong, Peizhe Lin, Qilong Wang, Rui Gao, Xianbin Kong, Long Yang
Summary: This paper reviews the research progress on the pathogenesis of the novel coronavirus (SARS-CoV-2), focusing on its pathogenic characteristics, loci of action, pathogenic mechanisms leading to immune response malfunction, as well as summarizing the pathological damage and manifestations it causes.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Virology
Chowdhury Nusaiba Binte Sayed Prapty, Raad Rahmat, Yusha Araf, Samiha Kamal Shounak, Noor-A-Afrin, Tanjim Ishraq Rahaman, Mohammad Jakir Hosen, Chunfu Zheng, Md. Golzar Hossain
Summary: The co-infection of SARS-CoV-2 and dengue virus is increasing in dengue-endemic regions, posing a threat of a co-epidemic. The comorbidity risk in co-infection cases is greater than that of a single viral infection. Although the pathophysiologies of the two infections differ, they have similar effects in the body, resulting in identical clinical symptoms in the case of co-infection. Proper differentiation and diagnosis of the infections are important, and specific biomarkers can be used for precise results. However, the treatment of co-infection cases is more complicated. Therefore, confirmation diagnosis of both COVID-19 and dengue should be mandatory to prevent health deterioration in individuals treated for a single infection. The literature on the epidemiology, pathophysiology, diagnosis, therapy, and management of SARS-CoV-2 and dengue virus co-infection is scarce. This review provides a summary of the epidemiology, pathogenesis, diagnosis, treatment, and management of co-infection cases, shedding light on appropriate approaches for patients.
REVIEWS IN MEDICAL VIROLOGY
(2023)
Article
Immunology
Panagiota Maravelia, Lars Frelin, Yi Ni, Noelia Caro Perez, Gustaf Ahlen, Neetu Jagya, Georg Verch, Lieven Verhoye, Lena Pater, Magnus Johansson, Anna Pasetto, Philip Meuleman, Stephan Urban, Matti Sallberg
Summary: This study introduces a novel immunotherapy targeting viral entry for chronic HBV/HDV infections, which aims to activate PreS1-specific T cells and antibodies to block HBV entry, showing promising results in preventing and modulating HBV infection.
JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Parasitology
Amelie Wallenhammar, Richard Lindqvist, Naveed Asghar, Sezin Gunaltay, Hans Fredlund, Ake Davidsson, Soren Andersson, Anna K. Overby, Magnus Johansson
PARASITES & VECTORS
(2020)
Article
Immunology
Richard Lindqvist, Ebba Rosendal, Elvira Weber, Naveed Asghar, Sarah Schreier, Annasara Lenman, Magnus Johansson, Gerhard Dobler, Malena Bestehorn, Andrea Kroeger, Anna K. Overby
JOURNAL OF NEUROINFLAMMATION
(2020)
Article
Microbiology
Pham-Tue-Hung Tran, Naveed Asghar, Urban Hoglund, Olivia Larsson, Lars Haag, Ali Mirazimi, Magnus Johansson, Wessam Melik
Article
Virology
Pham-Tue-Hung Tran, Naveed Asghar, Magnus Johansson, Wessam Melik
Summary: The study characterized the functions of LNP during flavivirus infection, showing that LNP plays a crucial role in vesicle generation and interacts with the NS4B protein. Depletion of LNP resulted in reduced virus replication, shedding new light on the interactions between flaviviruses and host factors.
Article
Microbiology
Pham-Tue-Hung Tran, Urban Hoglund, Olivia Larsson, Sofia Appelberg, Ali Mirazimi, Magnus Johansson, Wessam Melik
Summary: In this study, the use of RVPs based on the Kunjin strain of WNV (WNVKUN) as a potential WNV vaccine candidate was characterized. It was found that the inclusion of CCHFV Gn and Gc proteins in WNVKUN RVPs enhanced the immunogenicity, providing a possibility for the development of a future WNV vaccine.
Article
Gastroenterology & Hepatology
Rani Burm, Panagiota Maravelia, Gustaf Ahlen, Sandra Ciesek, Noelia Caro Perez, Anna Pasetto, Stephan Urban, Freya Van Houtte, Lieven Verhoye, Heiner Wedemeyer, Magnus Johansson, Lars Frelin, Matti Sallberg, Philip Meuleman
Summary: This study demonstrates the immunogenicity and effectiveness of preS1-HDAg immunotherapy in preventing HBV and HDV infections both in vitro and in vivo. The vaccine can complement current and future therapies for the control of chronic HBV and HDV infection.
Article
Immunology
Naveed Asghar, Wessam Melik, Katrine M. Paulsen, Bendikte N. Pedersen, Erik G. Bo-Granquist, Rose Vikse, Snorre Stuen, Soren Andersson, Ake Strid, Ashild K. Andreassen, Magnus Johansson
Summary: Flaviviruses pose a threat to public health, with tick-borne encephalitis being one of the major diseases caused by this virus. There is currently no antiviral treatment available, making vaccination the best preventive measure. A study aimed to develop an edible vaccine for tick-borne encephalitis, which would trigger a stronger immune response through oral delivery of viral antigens. However, the edible vaccine candidate did not provide immunity in mice during the challenge with the virus.
Article
Multidisciplinary Sciences
Abubakr A. M. Omer, Jorma Hinkula, Pham-Tue-Hung Tran, Wessam Melik, Elisa Zattarin, Daniel Aili, Robert Selegard, Torbjorn Bengtsson, Hazem Khalaf
Summary: Potent broad-spectrum antiviral agents are urgently needed to combat viral infections. Antimicrobial peptides, such as PLNC8 alpha beta, show high affinity for viral envelopes and can potentially be developed as effective and safe antiviral agents. PLNC8 alpha beta exhibits antiviral effects against various viruses and can efficiently eliminate infectious virus particles within a specific concentration range.
Article
Immunology
Anna Omazic, Amelie Wallenhammar, Elina Lahti, Naveed Asghar, Alexander Hanberger, Marika Hjertqvist, Magnus Johansson, Ann Albihn
Summary: Tick-borne encephalitis (TBE) is a severe tick-borne disease in Europe, mainly transmitted by tick bites. In this study, milk samples from Swedish dairy farms were tested and TBEV antibodies were found in some farms, indicating potential risks of alimentary TBEV infection due to consumption of unpasteurized milk, limited tick prophylaxis, and low TBE vaccination coverage.
COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES
(2023)
Article
Multidisciplinary Sciences
Lene Jung Kjaer, Magnus Johansson, Per-Eric Lindgren, Naveed Asghar, Peter Wilhelmsson, Hans Fredlund, Madeleine Christensson, Amelie Wallenhammar, Rene Bodker, Gunlog Rasmussen, Petter Kjellander
Summary: The incidence of tick-borne encephalitis (TBE) has been increasing in orebro County, south-central Sweden, but the reasons behind this trend are not well understood. This study analyzed TBE human case data, tick abundance, and environmental factors in different postal codes within the county to identify spatial patterns and potential drivers of TBE. The results showed a significant increase in TBE incidence in orebro County, with hotspots in the southern and northern parts and a cold spot in the central part. However, the models used had limited explanatory power for the observed pattern.
SCIENTIFIC REPORTS
(2023)
Article
Virology
Pham-Tue-Hung Tran, Abhilash Chiramel, Magnus Johansson, Wessam Melik
Summary: During flavivirus infection, ALIX and CHMP4A, members of the ESCRT protein machinery, are required for viral replication and assembly respectively. The recruitment of ESCRT proteins by NS3 through a specific L domain motif and the antiviral mechanism of ISG15 and HERC5 have been revealed.
JOURNAL OF VIROLOGY
(2022)
