Article
Biochemistry & Molecular Biology
Krzysztof Gomulka, Ewa Wojcik, Jacek Cezary Szepietowski
Summary: Atopic dermatitis is a chronic, highly pruritic, relapsing-remitting inflammatory skin disease. This study found that the serum levels of EDN and VEGF were significantly increased in patients with AD, while the level of PAF showed no significant difference. In AD patients, there was a positive correlation between the EDN level and the severity of the disease.
Letter
Medicine, General & Internal
Benjamin Sommer Thinggaard, Frederik Pedersen, Jakob Grauslund, Lonny Stokholm
Summary: This cohort study examines the use and forecast of intravitreal injections of vascular endothelial growth factor inhibitor in Denmark from 2007 through 2022.
Review
Biochemistry & Molecular Biology
Genevieve A. Secker, Natasha L. Harvey
Summary: The importance of lymphatic vessels in human diseases, the initiation of lymphatic vascular development during embryogenesis, and the role of VEGF/VEGFR signaling in driving the construction of lymphatic vessels were discussed in this review.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Clinical Neurology
Carlos Escudero, Jesenia Acurio, Eduardo Lopez, Andres Rodriguez, Antonia Benavente, Evelyn Lara, Steven J. Korzeniewski
Summary: Circulating VEGF levels were found to be elevated 24-48 hours after acute ischemic stroke and were associated with prognosis of moderate to severe disability at 6 months. Combining VEGF with interleukin 6 and C-reactive protein significantly improved predictive performance compared to using VEGF alone.
EUROPEAN JOURNAL OF NEUROLOGY
(2021)
Article
Biochemistry & Molecular Biology
Krzysztof Gomulka, Wojciech Medrala
Summary: This study aimed to investigate the serum levels of VEGF, PAF, and EDN in relation to disease activity and pruritus intensity in adult patients with CSU. The results showed higher levels of VEGF, PAF, and EDN in CSU patients compared with healthy subjects, but these cytokines were not significantly correlated with disease activity or pruritus intensity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Fuensanta Gomez-Bernal, Yolanda Fernandez-Cladera, Juan Carlos Quevedo-Abeledo, Maria Garcia-Gonzalez, Agustin F. Gonzalez-Rivero, Antonia De Vera-Gonzalez, Candelaria Martin-Gonzalez, Miguel A. Gonzalez-Gay, Ivan Ferraz-Amaro
Summary: This study analyzed the relationship between VEGF and sVEGFR with SLE disease-related features and found that serum levels of VEGF and sVEGFR in SLE patients were associated with disease damage, with musculoskeletal manifestations being the most commonly associated feature.
Article
Pharmacology & Pharmacy
Jun-Hei Chang, Chen-Chuan Cheng, Yen-Yu Lu, Cheng-Chih Chung, Yung-Hsin Yeh, Yao-Chang Chen, Satoshi Higa, Shih-Ann Chen, Yi-Jen Chen
Summary: VEGF reduces PV arrhythmogenesis by modulating sodium/calcium homeostasis through VEGFR-1/NOS signaling pathway.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2021)
Article
Nanoscience & Nanotechnology
Xiao-yu Dai, Li-jun Ren, Lang Yan, Ji-qian-zhu Zhang, Yi-fan Dong, Tao-lin Qing, Wen-jing Shi, Jin-feng Li, Fang-yuan Gao, Xiao-fang Zhang, Yi-jun Tian, Yu-ping Zhu, Jiang-bo Zhu, Ji-kuai Chen
Summary: This study demonstrated that MWCNTs can affect endothelial cell activity, disrupt tube formation and cell migration ability, subsequently affecting angiogenesis. Furthermore, the VEGF-Akt-eNOS axis plays a crucial role in MWCNT-induced endothelial cell injury.
Article
Hematology
Kathryn N. Phoenix, Zhichao Yue, Lixia Yue, Chunxia G. Cronin, Bruce T. Liang, Luke H. Hoeppner, Kevin P. Claffey
Summary: This study identifies PLC beta 2 as a key regulator of vascular permeability induced by VEGF, functioning through regulation of calcium flux and phosphatidylinositol 4,5-bisphosphate levels. Targeting PLC beta 2 could provide a novel approach to regulate vascular permeability in patients.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Prashant Jain, Chethan D. Shanthamurthy, Shani Leviatan Ben-Arye, Sharon Yehuda, Sharvani S. Nandikol, Hirekodathakallu Thulasiram, Vered Padler-Karavani, Raghavendra Kikkeri
Summary: A potential heparan sulfate (HS) ligand was discovered to target several growth factors using specific HS tetrasaccharide ligands. One tetrasaccharide ligand showed strong binding with the VEGF(165) growth factor, and in vitro experiments demonstrated the therapeutic potential of these synthetic HS ligands by inhibiting vascular endothelial cell proliferation, migration, and angiogenesis in the presence of VEGF(165) and the specific tetrasaccharides.
CHEMICAL COMMUNICATIONS
(2021)
Article
Clinical Neurology
Il-doo Kim, John W. Cave, Sunghee Cho
Summary: Obese mice with acute stroke showed increased mortality, infarct volume, swelling, and blood-brain barrier disruption, along with elevated VEGF-A and VEGFR2 expression. Aflibercept could reduce brain edema and vascular permeability, improving stroke outcomes in obese subjects.
Article
Chemistry, Multidisciplinary
Yang Liu, Linyu Long, Fanjun Zhang, Xuefeng Hu, Jieyu Zhang, Cheng Hu, Yunbing Wang, Jianguo Xu
Summary: The study introduced a GelMA microneedle platform for sustained and controlled local delivery of AAV-VEGF, which achieved homogeneous distribution and high transfection efficiency in ischemic brains. Results showed that MNs loaded with AAV-VEGF increased VEGF expression in the brain cortex and enhanced functional angiogenesis and neurogenesis.
JOURNAL OF CONTROLLED RELEASE
(2021)
Article
Multidisciplinary Sciences
Zhiyang Li, Heng Zhang, Yujie Sun, Zhuangzhuang Feng, Bijia Cui, Jingxia Han, Yinan Li, Huijuan Liu, Tao Sun
Summary: Angiogenesis plays a crucial role in embryologic development, growth, and tissue repair, but dysregulation of this process is a hallmark of various pathologies, including cancer. Existing methods for evaluating cell vascular formation are often limited to static analysis, leading to bias. Researchers have developed code scripts to study the dynamic angiogenesis process, allowing for the screening of drugs that can affect various parameters of vascular formation. Animal experiments have confirmed the efficacy of these drugs in inhibiting blood vessel formation, providing a new perspective for angiogenesis research and drug development.
Review
Biochemistry & Molecular Biology
Jean-Luc Wautier, Marie-Paule Wautier
Summary: The study of vascular permeability is important for understanding the mechanism that maintains the exchange between blood, tissues, and organs. Recent research has provided a better understanding of vascular cell functions and interactions between blood vessels. The identification of endothelial cell receptors and mediators and their role in homeostasis and disease has shed light on the maintenance of vascular permeability. Additionally, drugs and specific antibodies have been found to reduce the severity and mortality of diseases with enhanced vascular permeability.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Biomedical
Deepti Rana, Prasanna Padmanaban, Malin Becker, Fabian Stein, Jeroen Leijten, Bart Koopman, Jeroen Rouwkema
Summary: Given the need for control over evolving biochemical cues within synthetic matrices, this study focused on the incorporation of VEGF165 specific aptamers into cell-instructive polymer networks to regulate VEGF bioactivity. Bicomponent micropatterns were fabricated via photopatterning and demonstrated the retention of aptamers, molecular recognition, and VEGF sequestration. The study also revealed the ability of aptamer-tethered VEGF to spatiotemporally regulate vascular morphogenesis.
MATERIALS TODAY BIO
(2023)
Article
Biochemistry & Molecular Biology
Dong-Jae Jun, Marc M. Schumacher, Seonghwan Hwang, Lisa N. Kinch, Nick V. Grishin, Russell A. DeBose-Boyd
JOURNAL OF LIPID RESEARCH
(2020)
Review
Biochemistry & Molecular Biology
Archana S. Bhat, Richard Dustin Schaeffer, Lisa Kinch, Kirill E. Medvedev, Nick V. Grishin
CURRENT OPINION IN STRUCTURAL BIOLOGY
(2020)
Editorial Material
Multidisciplinary Sciences
Samuel Pena-Llopis
Summary: During the early days of the pandemic, I played a game called Keep Talking and Nobody Explodes with my research group through teleconferencing. In the game, I acted as the defuser with the bomb, while my team members played the role of experts, working together to defuse the virtual bomb through communication and collaboration.
Article
Multidisciplinary Sciences
Jennifer Niersch, Silvia Vega-Rubin-de-Celis, Anna Bazarna, Svenja Mergener, Verena Jendrossek, Jens T. Siveke, Samuel Pena-Llopis
Summary: Synonymous mutations are often disregarded in genomic analyses, but they can cause changes in protein function in certain genes, affecting the prognosis of disease patients. Therefore, careful scrutiny of synonymous mutations near cancer genes is crucial for accurate precision medicine.
Article
Biochemistry & Molecular Biology
Svenja Mergener, Jens T. Siveke, Samuel Pena-Llopis
Summary: The study found that monosomy of chromosome 3 (M3) and mutations in BAP1 are associated with higher resistance to MEK inhibitors in uveal melanoma. Reconstitution of BAP1 was unable to restore sensitivity to MEK inhibition. Comparison of UM tumors with mutations in BAP1 and wild-type BAP1 from TCGA showed clear differentiation in overall and progression-free survival.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Lisa N. Kinch, R. Dustin Schaeffer, Andriy Kryshtafovych, Nick V. Grishin
Summary: An evolutionary-based definition and classification of target evaluation units (EUs) were presented for CASP14, with targets split into categories based on existing templates and server performance. Analysis of sequence and structure similarity, as well as server performance, highlighted trends in target difficulty for the 84 experimental models submitted. The study assigned EUs to tertiary structure assessment categories based on their evolutionary relationship to existing ECOD fold space, showing overlapping clusters and the complexity of server performance in predicting target structures.
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS
(2021)
Article
Biochemistry & Molecular Biology
Lisa N. Kinch, Jimin Pei, Andriy Kryshtafovych, R. Dustin Schaeffer, Nick Grishin
Summary: This report summarizes the tertiary structure prediction assessment of difficult modeling targets in CASP14, with the top-performing AlphaFold2 group providing high quality models. Despite significant progress in protein structure prediction, challenges remain with flexible regions and obligate oligomeric assemblies. Performance-based PCA and heatmap clusters offer insight into target difficulties and successful structure prediction methodologies.
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS
(2021)
Article
Biochemistry & Molecular Biology
R. Dustin Schaeffer, Lisa Kinch, Andriy Kryshtafovych, Nick Grishin
Summary: This study evaluated the accuracy of CASP14 models in structure predictions, focusing on the difficulty of structure assembly in multidomain targets, with AlphaFold2 performing exceptionally well.
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS
(2021)
Article
Multidisciplinary Sciences
Minkyung Baek, Frank DiMaio, Ivan Anishchenko, Justas Dauparas, Sergey Ovchinnikov, Gyu Rie Lee, Jue Wang, Qian Cong, Lisa N. Kinch, R. Dustin Schaeffer, Claudia Millan, Hahnbeom Park, Carson Adams, Caleb R. Glassman, Andy DeGiovanni, Jose H. Pereira, Andria V. Rodrigues, Alberdina A. van Dijk, Ana C. Ebrecht, Diederik J. Opperman, Theo Sagmeister, Christoph Buhlheller, Tea Pavkov-Keller, Manoj K. Rathinaswamy, Udit Dalwadi, Calvin K. Yip, John E. Burke, K. Christopher Garcia, Nick V. Grishin, Paul D. Adams, Randy J. Read, David Baker
Summary: Through the three-track network, we achieved accuracies approaching those of DeepMind in CASP14, enabling rapid solution of challenging x-ray crystallography and cryo-electron microscopy structure modeling problems, and providing insights into the functions of proteins with currently unknown structure.
Article
Cell Biology
Luca Hegedues, Kata D. Szuecs, Matthias Kudla, Julian Heidenreich, Verena Jendrossek, Samuel Pena-Llopis, Tamas Garay, Andras Czirok, Clemens Aigner, Till Ploenes, Silvia Vega-Rubin-de-Celis, Balazs Hegedues
Summary: This study investigated the effect of Nintedanib on SRC kinase, one of its targets, in MPM cells. The results showed that Nintedanib did not inhibit SRC activation and even increased SRC phosphorylation in some MPM cell lines. However, combination treatment with the SRC inhibitor Dasatinib reversed this effect. The study also revealed that combination treatment promoted autophagy in MPM cells, which was independent of the AKT/mTOR and ERK pathways.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Oncology
Chen Yao, Tian Zhang, Tuoqi Wu, James Brugarolas
Summary: Immunotherapy has had a significant impact on renal cell carcinoma (RCC). Combining anti-angiogenic agents with immune checkpoint inhibitors has transformed patient outcomes. The challenges now include patient selection, resistance mechanisms, and optimizing treatment sequencing. Further research may pave the way for the next generation of therapies.
CLINICAL CANCER RESEARCH
(2022)
Article
Oncology
Kangsan Kim, Huocong Huang, Pravat Kumar Parida, Lan He, Mauricio Marquez-Palencia, Tanner C. Reese, Payal Kapur, James Brugarolas, Rolf A. Brekken, Srinivas Malladi
Summary: Cell competition leads to the displacement of latent metastatic cells from the primary tumor, with disruption of this process resulting in reduced metastatic incidence. Kidney cancer has enriched tumor subclones with latent metastatic traits, which are associated with rapid onset of metastases and reduced disease-free survival. The findings highlight the importance of cell competition in shaping metastatic latency and relapse.
Article
Biochemistry & Molecular Biology
Ranadip Mandal, Monika Raab, Franz Roedel, Andrea Kraemer, Izabela Kostova, Samuel Pena-Llopis, Bjorn Haeupl, Thomas Oellerich, Khayal Gasimli, Mourad Sanhaji, Sven Becker, Klaus Strebhardt
Summary: This study identifies a new mechanism where nuclear Caspase-8 inhibits the activity of CDK9, leading to poor prognosis and chemoresistance in cervical cancer. Caspase-8-deficient cervical cancer cells show increased resistance to CDK9 inhibitors, but combination with Cisplatin can synergistically overcome this resistance.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Correction
Biochemistry & Molecular Biology
Ranadip Mandal, Monika Raab, Franz Rodel, Andrea Kramer, Izabela Kostova, Samuel Pena-Llopis, Gioele Medici, Bjoern Haupl, Thomas Oellerich, Khayal Gasimli, Mourad Sanhaji, Sven Becker, Klaus Strebhardt
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Cell Biology
Ben Zhao, Laura Dierichs, Jiang-Ning Gu, Marija Trajkovic-Arsic, Ralf Axel Hilger, Konstantinos Savvatakis, Silvia Vega-Rubin-de-Celis, Sven-Thorsten Liffers, Samuel Pena-Llopis, Diana Behrens, Stephan Hahn, Jens T. Siveke, Smiths S. Lueong
CELL DEATH DISCOVERY
(2020)