Article
Multidisciplinary Sciences
Gema Alama-Bermejo, Jesus S. Hernandez-Orts, Martin Garcia-Varela, Alejandro Oceguera-Figueroa, Hana Peckova, Ivan Fiala
Summary: This study was the first to explore the diversity of myxozoan parasites in fishes from the Neotropical region of Mexico, identifying over 40 putative new species. The findings highlight the potential economic and conservation implications of these parasites.
SCIENTIFIC REPORTS
(2023)
Review
Plant Sciences
Mariana Cansian Sattler, Stefanie Cristina de Oliveira, Maria Andreia Correa Mendonca, Wellington Ronildo Clarindo
Summary: This article reviews the cytogenetics of Coffea, including karyotype characterization, DNA content, and genomic integration. The research highlights the diploidy of most Coffea species, the tetraploidy of C. arabica, and the polyploidy of other hybrids. The cytogenomic data provide insights into the phylogenetic relations and genomic origin of Coffea, as well as the role of polyploidy in C. arabica. The article also discusses the potential of the 'Hibrido de Timor' as a source of resistance genes and a model for polyploidy research.
Article
Zoology
David Sadilek, Jitka Vilimova, Tomas Urfus
Summary: The study analyzed genome size and the position of 18S ribosomal DNA in different species from the family Nabidae, revealing distinct chromosomal characteristics and significant differences in nuclear DNA content among the three genera studied. Notably, the results strongly support the theory of an autosomal polyploidy event in the evolution of the genus Himacerus.
ZOOLOGICAL JOURNAL OF THE LINNEAN SOCIETY
(2021)
Article
Biology
Mariannah Pravatti Barcellos de Oliveira, Rafael Kretschmer, Geize Aparecida Deon, Gustavo Akira Toma, Tariq Ezaz, Caio Augusto Gomes Goes, Fabio Porto-Foresti, Thomas Liehr, Ricardo Utsunomia, Marcelo de Bello Cioffi
Summary: In this study, the dynamics of satellite DNAs (SatDNAs) accumulation and elimination during W chromosome differentiation in Triportheus fish were investigated. The majority of repetitive sequences were eliminated as speciation occurred in five additional Triportheus species. While the W chromosomes continued to degenerate, the Z chromosomes in some species began to accumulate TauSatDNAs. Species-specific SatDNAs were likely amplified in the heterochromatic regions of both Z and W chromosomes in each species. Thus, the W chromosomes of various Triportheus species underwent significant evolutionary changes within a short period of time (15-25 Myr) after divergence.
Review
Cell Biology
Thomas Liehr
Summary: Chromosomal heteromorphisms (CHs) are part of genetic variation in humans. Recent research has raised questions about the potential role of CHs in cancer susceptibility. However, the current routine cytogenetic analysis does not give CHs sufficient consideration, which highlights the need for more attention in future research, particularly in the study of solid tumors.
Article
Entomology
Mara Garcia Tavares, Riudo de Paiva Ferreira, Natalia Martins Travenzoli, Denilce Meneses Lopes
Summary: The study compared the karyotype, rDNA cluster number and localization, and repetitive DNA sequence distribution in different populations of T. spinipes from nine locations, showing diverse karyotypic formulas and heterochromatin preference for centromeric regions. Microsatellite probe signals were mainly located in euchromatic regions of all chromosomes, while 18S rDNA clusters varied in number among different chromosomal pairs. Cytogenetic variations were observed in T. spinipes along geographic distribution, particularly in scattered colonies.
Article
Plant Sciences
Adriano Silva Dos Santos, Karina de Cassia Faria
Summary: This study characterized the chromosome composition of Rhogeessa hussoni, revealing a chromosome constitution of 2n=52 and NF=54. The research also identified regions of heterochromatin and sites of ribosomal DNA in this species.
Article
Developmental Biology
Carlos Alexandre Fernandes, Leonardo Marcel Paiz, Diovani Piscor, Mariane Gavazzoni, Luciana Andreia Borin de Carvalho, Ana Luiza de Brito Portela-Castro, Vladimir Pavan Margarido
Summary: This study investigated the cytogenetic and molecular data of two Farlowella hahni populations, revealing chromosomal rearrangements and differences in rDNA sites. Fluorescence in situ hybridization showed variation in 18S rDNA and 5S rDNA sites between populations, while mitochondrial sequence analyses were unable to differentiate the two populations.
Article
Biotechnology & Applied Microbiology
Abigail Hui En Chan, Naowarat Saralamba, Sompob Saralamba, Jiraporn Ruangsittichai, Urusa Thaenkham
Summary: This study presented novel and broadly applicable primers using the mitochondrial 12S and 16S rRNA genes for molecular identification of parasitic trematodes. The results indicated that these primers have sufficient resolution to discriminate closely related species and achieve accurate species identification through phylogenetic placements. The convenience and applicability of the newly designed primers and the adequate genetic variation of the mitochondrial rRNA genes make them useful as complementary markers for trematode molecular-based studies.
Article
Ecology
Yan Torres, Vicente V. Faria, Patricia Charvet
Summary: Potamotrygoninae is a group of stingrays that are well suited to South American freshwater ecosystems. This review discusses the recent advances in genetic studies of this group and highlights the potential future directions for research. Despite the increasing number of studies, there are still unanswered questions, particularly regarding the high genetic similarity observed among Potamotrygon species. The application of genomics techniques may provide insights into the complex issues surrounding freshwater stingrays.
ENVIRONMENTAL BIOLOGY OF FISHES
(2022)
Article
Genetics & Heredity
Maria Laura Gonzalez, Jorge Oscar Chiapella, Juan Domingo Urdampilleta
Summary: Various families of satellite DNA were found in the heterochromatin regions of Deschampsia, contributing to lineage differentiation. Differentiation between types of satellite DNA is linked to sequence identity, array size, and chromosomal location. Despite interspecific variations in satellite DNA loci, the general distribution pattern of each family remains consistent, suggesting non-random distribution in genomes with variation mainly in array size and loci number.
FRONTIERS IN GENETICS
(2021)
Article
Cell Biology
Vanessa C. S. Oliveira, Marie Altmanova, Patrik F. Viana, Tariq Ezaz, Luiz A. C. Bertollo, Petr Rab, Thomas Liehr, Ahmed Al-Rikabi, Eliana Feldberg, Terumi Hatanaka, Sebastian Scholz, Alexander Meurer, Marcelo de Bello Cioffi
Summary: This study analyzed the karyotypes of eight species from the Alligatoridae family using various cytogenetic techniques. The research revealed significant differences in chromosome number and morphology between Caimaninae and Alligatorinae species, suggesting fusion/fission rearrangements. Sequence divergence among caiman genomes was also observed, with Paleosuchus exhibiting the highest level of divergence.
Article
Biochemistry & Molecular Biology
Gustavo A. Toma, Natalia dos Santos, Rodrigo dos Santos, Petr Rab, Rafael Kretschmer, Tariq Ezaz, Luiz A. C. Bertollo, Thomas Liehr, Fabio Porto-Foresti, Terumi Hatanaka, Alongklod Tanomtong, Ricardo Utsunomia, Marcelo B. Cioffi
Summary: The study investigated the karyotypes of different color phenotypes in Scleropages formosus using molecular cytogenetic techniques. The results revealed population genetic structure and microstructure differences in the karyotypes of the color phenotypes, but did not conclusively support the hypothesis of discrete lineages or evolutionary units among them.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Jonathan O. Nelson, Alyssa Slicko, Yukiko M. Yamashita
Summary: A specific retrotransposon called R2 is essential for maintaining the copy number of ribosomal DNA (rDNA) in Drosophila male germline. R2 induces double-stranded DNA breaks at rDNA copies, triggering their repair and preventing copy number loss. This study reveals the important role of retrotransposons in host fitness.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Genetics & Heredity
Caio Augusto Gomes Goes, Natalia dos Santos, Pedro Henrique de Mira Rodrigues, Jose Henrique Forte Stornioli, Amanda Bueno da Silva, Rodrigo Zeni dos Santos, Jhon Alex Dziechciarz Vidal, Duilio Mazzoni Zerbinato de Andrade Silva, Roberto Ferreira Artoni, Fausto Foresti, Diogo Teruo Hashimoto, Fabio Porto-Foresti, Ricardo Utsunomia
Summary: Satellite DNAs (satDNAs) are repeated sequences located on heterochromatin and evolve by concerted evolution. In this study, two satellitomes of Characiformes fish were characterized, showing conservation between species despite divergence 30 million years ago. The results suggest that long-life cycles and few genomic changes may slow down satDNA differentiation rates.
Editorial Material
Cell Biology
VladimirA. Trifonov, Marcelo de Bello Cioffi, Thomas Liehr
CYTOGENETIC AND GENOME RESEARCH
(2021)
Article
Cell Biology
Ikuo Miura, Foyez Shams, Si-Min Lin, Marcelo de Bello Cioffi, Thomas Liehr, Ahmed Al-Rikabi, Chiao Kuwana, Kornsorn Srikulnath, Yuya Higaki, Tariq Ezaz
Summary: Translocations between sex chromosomes and autosomes can generate multiple sex chromosome systems. In a Taiwanese brown frog, a unique multiple sex chromosome system was discovered, involving three different chromosome pairs with translocations occurring among potential sex-determining chromosomes, including orthologs of sex-determining genes in various vertebrates. This rare case suggests sex-specific, nonrandom translocations, shedding light on the evolutionary significance of multiple sex chromosome systems.
Article
Agriculture, Dairy & Animal Science
Rafael Kretschmer, Benilson Silva Rodrigues, Suziane Alves Barcellos, Alice Lemos Costa, Marcelo de Bello Cioffi, Analia del Valle Garnero, Ricardo Jose Gunski, Edivaldo Herculano Correa de Oliveira, Darren K. Griffin
Summary: Detailed chromosome studies of the Saffron finch revealed a high conservation of chromosomes, with SSR mainly accumulating in microchromosomes and the short arms of Z chromosome. Comparisons with other avian species provided insights into the chromosome organization and evolution of the Saffron finch genome. The contrasting rates of intrachromosomal changes and the role of SSR accumulation in karyotype reorganization were highlighted.
Review
Biology
Alexandr Sember, Petr Nguyen, Manolo F. Perez, Marie Altmanova, Petr Rab, Marcelo de Bello Cioffi
Summary: This review provides insights into the characteristics and evolutionary trends of multiple sex chromosome systems in teleost fishes, pointing out the predominance of male-heterogametic systems and the possible constraints on sex chromosome turnover in female-heterogametic systems. Furthermore, the study highlights the importance of broader inter-population sampling in understanding the evolution of multiple sex chromosomes in fishes.
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Rafael Kretschmer, Caio Augusto Gomes Goes, Luiz Antonio Carlos Bertollo, Tariq Ezaz, Fabio Porto-Foresti, Gustavo Akira Toma, Ricardo Utsunomia, Marcelo de Bello Cioffi
Summary: This study investigated the composition and role of satellite DNA (satDNA) in the evolution of sex chromosomes in Triportheus species. The results revealed differences in satDNA accumulation on the Z chromosome among different species and different homogenization rates between the Z and W chromosomes. The most abundant satDNA family was found to be localized in the centromeres and potentially played a role in centromere evolution.
Article
Developmental Biology
Rodrigo Petry Correa de Sousa, Carolina Pinheiro Vasconcelos, Nayara Furtado do Rosario, Aldemir Branco De Oliveira-Filho, Edivaldo Herculano Correa de Oliveira, Marcelo de Bello Cioffi, Marcelo Vallinoto, Glaucia Caroline Silva-Oliveira
Summary: By analyzing the karyotype and distribution of repetitive DNA in two Elopiformes species, the study found that microsatellite sequences are primarily associated with heterochromatin and may contribute to chromosome structure, while transposable elements have different distributions in the two species, indicating potential interspecific differences in their function. Comparative genome mapping provides new insights into the structure and organization of repetitive sequences in the Elopiformes genome.
Article
Genetics & Heredity
Renata Luiza Rosa de Moraes, Francisco de Menezes Cavalcante Sassi, Luiz Antonio Carlos Bertollo, Manoela Maria Ferreira Marinho, Patrik Ferreira Viana, Eliana Feldberg, Vanessa Cristina Sales Oliveira, Geize Aparecida Deon, Ahmed B. H. Al-Rikabi, Thomas Liehr, Marcelo de Bello Cioffi
Summary: The study combined classical and molecular cytogenetic analyses to investigate the karyotypes of five Pyrrhulina species. It found significant differences in chromosome numbers and positions among the species, while repetitive DNA content remained relatively consistent. The results suggest that karyotype differentiation in Pyrrhulina is primarily driven by structural rearrangements.
FRONTIERS IN GENETICS
(2021)
Article
Biology
Fernando H. S. de Souza, Francisco de M. C. Sassi, Pedro H. N. Ferreira, Luiz A. C. Bertollo, Tariq Ezaz, Thomas Liehr, Manolo F. Perez, Marcelo B. Cioffi
Summary: This study investigated the relationship between the evolution of multiple sex chromosomes together with allopatry and genetic diversity and speciation in the fish species Erythrinus erythrinus. The presence of multiple sex chromosomes was hypothesized to contribute to genetic differentiation and potentially accelerate speciation. The results showed high genetic differentiation among populations, with long-distance allopatry as the main agent of genetic divergence.
Article
Genetics & Heredity
Geize Aparecida Deon, Larissa Glugoski, Francisco de Menezes Cavalcante Sassi, Terumi Hatanaka, Viviane Nogaroto, Luiz Antonio Carlos Bertollo, Thomas Liehr, Ahmed Al-Rikabi, Orlando Moreira-Filho, Marcelo de Bello Cioffi, Marcelo Ricardo Vicari
Summary: This study investigates the differentiation processes and evolutionary history of the rare XX/XY1Y2 sex chromosome system in Harttia species. By using whole chromosome painting probes, the study reveals the existence of homeologous chromosome blocks and the impact of Robertsonian fusions and fissions on sex chromosome origins. The data also support the presence of unstable genomic sites promoting chromosomal differentiation and remodeling within the genus Harttia.
FRONTIERS IN GENETICS
(2022)
Article
Biochemistry & Molecular Biology
Geize Aparecida Deon, Larissa Glugoski, Terumi Hatanaka, Francisco de Menezes Cavalcante Sassi, Viviane Nogaroto, Luiz Antonio Carlos Bertollo, Thomas Liehr, Ahmed Al-Rikabi, Orlando Moreira Filho, Marcelo de Bello Cioffi, Marcelo Ricardo Vicari
Summary: This study investigates the chromosomal rearrangements in the Neotropical armored catfish genus Harttia using powerful tools such as comparative whole chromosome painting. The results reveal the importance of translocation events in the karyotype evolution of Harttia species, as well as the independent origin of two types of multiple sex chromosomes.
GENETICS AND MOLECULAR BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Karlla Danielle Jorge Amorim, Gideao Wagner Werneck Felix da Costa, Marcelo de Bello Cioffi, Alongklod Tanomtong, Luiz Antonio Carlos Bertollo, Wagner Franco Molina
Summary: The karyotype evolution of Epinephelidae fits well with the expansion and geographic contingencies of its clades, providing a more complex and diverse scenario than previously assumed.
GENETICS AND MOLECULAR BIOLOGY
(2021)
Article
Plant Sciences
Sukhonthip Ditcharoen, Sudarat Khensuwan, Kriengkrai Seetapan, Phongphan Soonthornvipat, Chatmongkon Suwannapoom, Krit Pinthong, Sampan Tongnunui, Marcelo de Bello Cioffi, Thomas Liehr, Alongklod Tanomtong, Weerayuth Supiwong
Summary: The first chromosomal analysis of Salween shovelhead catfish (Sperata acicularis) revealed a diploid chromosome number of 56 with a karyotype composed of different types of chromosomes and unidentified sex chromosomes. Additionally, the distribution patterns of microsatellite repeats differed on the chromosomes.
Article
Cell Biology
Jose Souza, Erika Guimaraes, Vanessa Pinheiro-Figliuolo, Marcelo B. Cioffi, Luiz A. C. Bertollo, Eliana Feldberg
Summary: The study investigated the karyotype and cytogenetic features of pike characin, Ctenolucius hujeta, revealing similarities with Boulengerella species but also unique chromosomal marker distributions. The colocalization of the 18S and 5S rDNA genes is identified as a distinctive characteristic of the C. hujeta genome. Additionally, the research supports a close relationship between the families Ctenoluciidae and Lebiasinidae.
CYTOGENETIC AND GENOME RESEARCH
(2021)
Article
Marine & Freshwater Biology
Simiao Alefe Soares da Silva, Paulo Augusto de Lima-Filho, Clovis Coutinho da Motta-Neto, Gideao Wagner Werneck Felix da Costa, Marcelo de Bello Cioffi, Luiz Antonio Carlos Bertollo, Wagner Franco Molina
Summary: The order Gobiiformes consists of over 2200 species and is considered one of the most diverse groups among teleost fishes. This study expanded cytogenetic data for four neotropical species in the Eleotridae family and used a meta-analytical approach to explore the karyotype diversification in relation to biological aspects.
MARINE LIFE SCIENCE & TECHNOLOGY
(2021)
Article
Plant Sciences
Pun Yeesin, Phichaya Buasriyot, Sukhonthip Ditcharoen, Patcharaporn Chaiyasan, Chatmongkon Suwannapoom, Sippakorn Juntaree, Sitthisak Jantarat, Sucheela Talumphai, Marcelo de Bello Cioffi, Thomas Liehr, Alongklod Tanomtong, Weerayuth Supiwong
Summary: The karyotypes of four catfish species in genus Mystus were analyzed using conventional and molecular techniques, revealing distinct chromosome complements, karyotype formulas, and NOR distribution patterns. The use of microsatellite probes provided insights into the species-specific distribution patterns of repeat sequences.
COMPARATIVE CYTOGENETICS
(2021)
Article
Microscopy
Shengli Zhang, Ying Wang, Bowei Wang, Yi Zeng, Jiani Li, Xingyue Wang, Cuihua Hu, Zhankun Weng, Zuobin Wang
Summary: Curcumin was found to have effects on the activity, migration, and mitochondrial membrane potential of malignant hepatocytes and their mitochondria. The findings from both optical microscopy and atomic force microscopy studies confirm these effects on cell morphology and biomechanics.
Article
Microscopy
Rutika Sansaria, Krishanu Dey Das, Alwin Poulose
Summary: This paper presents a method for quantifying Golgi dispersion images and classifying them using machine learning models. By applying automated image processing and segmentation, extracting features, and using different classifiers, accurate classification of Golgi images is achieved.
Article
Microscopy
Marjorie da Silva, Cintia Eleonora Lopes Justino, Fernando Henrique Carnimeo, Fernando Barbosa Noll, Johan Billen
Summary: This article describes the secretory glands in females of two species of Myzinum and discusses their homology with other glands in the Hymenoptera order. The study finds that the three glands, instead of the commonly believed two, are present in Myzinum species. However, the limited research on the structure, function, and evolution of female accessory glands in Hymenoptera prevents conclusive statements.
Article
Microscopy
Ping Hao, Wen Yin, Xi Chen, Shuangshuang Qin, Yue Yu, Yuan Yuan, Xiaoyu Quan, Bing Hu, Shouhai Chen, Yi Wu
Summary: This study confirmed the secretion of nano-scale exosomes-multivesicular bodies and the interaction of high endothelial cells (HECs) with lysosomes and mitochondria in the small intestine of laying birds. Immunohistochemistry and immunofluorescence results showed the strong immunoreactivity of exosome-related proteins and autophagic-related proteins in the lamina propria of the small intestine. These findings reveal an innovative communication method among HECs in the small intestine.
Article
Microscopy
Stefan Talu, Robert S. Matos, Henrique Duarte da Fonseca Filho, Daniela Predoi, Simona Liliana Iconaru, Carmen Steluta Ciobanu, Liliana Ghegoiu
Summary: This study developed composite layers based on magnesium-doped hydroxyapatite and chitosan, and demonstrated their good biocompatibility and antifungal effects against osteosarcoma cells. The attachment and growth of the cells on the composite layers were promoted, while the growth of Candida albicans was inhibited. The morphological analysis revealed complex 3D patterns on the cancer cell surfaces on the magnesium-containing composite layers.
