Article
Neurosciences
Natalia Merkulyeva, Aleksandr Mikhalkin, Anna Kostareva, Tatyana Vavilova
Summary: This study focused on the postnatal development of perigeniculate neurons and found different neuronal subpopulations in kittens and adult cats. These subpopulations showed differences in morphology and neurochemical characteristics, which may have implications for the development of thalamocortical processing.
JOURNAL OF COMPARATIVE NEUROLOGY
(2022)
Article
Anatomy & Morphology
Xinlu Li, Geng Lin, Tongtong Liu, Ning Zhao, He Xu, Huaqin Wang, Wei Zheng
Summary: The expression and distribution of BAG3 in the brain of C57BL/6 mice show a significant decline shortly after birth, followed by minimal differences in young and middle-aged mice, and then a significant increase in aged mice at 18 months. This suggests that BAG3 may be stable in young and middle-aged mice, but unstable in aged mice.
BRAIN STRUCTURE & FUNCTION
(2021)
Article
Pharmacology & Pharmacy
Liangqin Shi, Hongfei Li, Zheng Wang, Weiming Liu, Xiao Wu, Jiali Li, Zhong Jin, Wangming Hu, Qiru Guo, Lingling Zhang, Yang Wang, Bing Liang, Song Zhu, Haidi Zhang, Huan Xu, Lan Yang, Riyi Shi, Yong Wang
Summary: This study aimed to investigate whether and how andrographolide (ANP) regulates embryonic neuronal development. The results showed that ANP inhibits the maturation of embryonic neurons by suppressing proliferation and migration, and interfering with the interaction between PFKFB3, NeuN, and TBR1. This suggests that ANP suppresses the expression of PFKFB3 and interrupts the interaction between TRB1 and NeuN, thereby inhibiting murine embryonic neuronal development.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2023)
Article
Environmental Sciences
Yipeng Cheng, Jiaqi Zhang, Haruka Nakano, Naofumi Ueyama, Hisayuki Arakawa
Summary: Microplastics are a significant ocean pollutant and are widely spread across the globe. Hence, there is a need to enhance our understanding of microplastic levels in different environments. However, current detection methods for microplastics are labor-intensive and expensive. In this study, we developed a fluorescence staining technique using coumarin 6 and evaluated its efficacy. By using a mixture of acetone and ethanol as a solvent, we successfully stained 10 different types of plastics with coumarin 6. The fluorescence peak for coumarin 6 staining was around 500 nm for each plastic type. The optimal immersion time and coumarin 6 concentration for staining were found to be 60 min and 1 mg L-1, respectively. Using this technique, we were able to stain all microplastic samples collected from seawater in Tokyo Bay.
MARINE POLLUTION BULLETIN
(2023)
Article
Biochemistry & Molecular Biology
Hyun Seung Shin, Soo Min Choi, Seung Hyun Lee, Ha Jung Moon, Eui-Man Jung
Summary: In this study, a novel early life stress (ELS) model was established, which led to negative effects on the offspring's brain and behavioral development, including anxiety, depression, social deficits, and memory impairment. The novel model induced more severe depression-like behavior and memory impairment compared to the established ELS model. Additionally, the novel ELS model caused upregulation of arginine vasopressin expression and downregulation of GABAergic interneuron markers in the mice's brains.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Lulu Wang, Mingdong Tang, Wenwen Huang, Jinjie An, Xiaomin Liu, Hongbo Gao
Summary: Immunofluorescence staining is an important method for detecting protein localization in cells, often used in the study of chloroplast division proteins. Researchers have developed a simplified method by breaking plant leaf tissues with a serrated blade, directly staining the samples, and lysing the tissue into separable cells, allowing for clear observation of the target protein's localization.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Multidisciplinary Sciences
Hiroyuki Sasaki, Hisako Arai, Emi Kikuchi, Hideki Saito, Keiko Seki, Takeshi Matsui
Summary: The use of Mayer's hematoxylin followed by Reynold's lead citrate solution is suggested as a reliable and promising alternative to Uranyl acetate (UA) staining for ultrathin sections in biological electron microscopy.
SCIENTIFIC REPORTS
(2022)
Article
Plant Sciences
Lulu Wang, Yajuan Chen, Di Niu, Mingdong Tang, Jinjie An, Shanshan Xue, Xiaomin Liu, Hongbo Gao
Summary: Immunofluorescence staining is widely used in the subcellular localization study of proteins. A tissue-chopping-based method for immunofluorescence staining of chloroplast proteins is improved by direct observation of stained tissues using a confocal microscope without tissue lysis and by maintaining a low temperature throughout the process. This method has been successfully applied to the staining of FtsZ1 in wildtype and mutant Arabidopsis, as well as in other plants, leading to further improvement of the tissue-chopping-based immunofluorescence staining method.
Article
Andrology
Mehmet Ozbek, Feyzullah Beyaz, Emel Ergun, Levent Ergun, Harun Karaca, Ahmet Cabir, Yahy Abood Kareem Alesawi
Summary: This study investigates the expression of calcium-binding proteins and neural cell markers in the rat testis and epididymis during postnatal development. The results show that these proteins have differential expression patterns in different stages and may play important roles in reproductive physiology.
Article
Environmental Sciences
Pham Le Quoc, Maria Fokina, Daria M. Martynova, Roman O. Olekhnovich, Mayya Uspenskaya
Summary: Plastic pollution has garnered much attention, but its impact on the environment and human health requires urgent assessment through experimental studies. However, limited studies exist due to the lack of standardized methods for evaluating their effects on living organisms. This article presents a process for manufacturing and staining PVC microparticles for use in biological and ecological experiments.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Chemistry, Analytical
Nazan Altun, Martin F. Hervello, Felipe Lombo, Pelayo Gonazlez
Summary: Currently, the lack of reference methods is hindering the full potential of spectral imaging, despite its ability to provide valuable information. This study proposes a staining-based reference method with digital image treatment for spectral imaging, aiming to predict the presence of biofilms in a fast, efficient, contactless, and non-invasive way.
Article
Plant Sciences
Conor J. C. Simpson, Pallavi Singh, Deedi E. O. Sogbohossou, M. Eric Schranz, Julian M. Hibberd
Summary: C-4 photosynthesis has evolved multiple times in angiosperms and usually involves changes to the biochemistry, cell biology, and development of leaves. One common modification in C-4 plants is an increase in vein density, which results in a larger proportion of bundle sheath cells in the leaf. This study presents a high-throughput method of quantifying vein density using starch staining, which allows for rapid phenotyping in C-4 species. The method was successfully applied to detect significant variation in vein density in Gynandropsis gynandra, but not in genotypically diverse lines of Zea mays.
PLANT CELL AND ENVIRONMENT
(2023)
Review
Biochemistry & Molecular Biology
Amanda J. Price, Andrew E. Jaffe, Daniel R. Weinberger
Summary: Schizophrenia's etiology is likely related to disruptions in early development, altering brain maturation and resulting in abnormal function in early childhood and adulthood. Cellular identity and development are governed by genomic regulation, which may reflect genetic and environmental influences, making investigating omic changes in diverse cells a valuable avenue for schizophrenia research.
MOLECULAR PSYCHIATRY
(2021)
Article
Behavioral Sciences
Dongming Guo, Yang Xu, Yingge Wang, Xiaolin Zhong, Zhenghai Liu, Suyun Li, Xiaofan Xu, Jingwen Zhang, Tianqing Xiong, Wenyu Cao, Jingyan Liang
Summary: This study investigated whether a TRPV4 blocker and knockdown of TRPV4 could improve cognitive behavior affected by pyroptotic processes. The results showed that both the TRPV4 blocker and TRPV4 knockdown inhibited pyroptosis in the hippocampus of mice treated with lipopolysaccharide (LPS) and improved cognitive behavior.
BEHAVIOURAL BRAIN RESEARCH
(2023)
Review
Pharmacology & Pharmacy
Kiri T. Granger, Michael Sand, Sheryl Caswell, Lorena R. Lizarraga-Valderrama, Jennifer H. Barnett, Paula M. Moran
Summary: For patients and clinicians, the challenge of managing psychotic symptoms is finding a treatment that balances efficacy and side effects. Karuna Therapeutics' recent Phase III study shows promise for a non-dopamine-based treatment for schizophrenia with reduced side effects. This success offers new hope and highlights lessons in schizophrenia drug development methodology.
DRUG DISCOVERY TODAY
(2023)
Article
Endocrinology & Metabolism
Tristan Struja, Marina Kaeslin, Fabienne Boesiger, Rebecca Jutzi, Noemi Imahorn, Alexander Kutz, Luca Bernasconi, Esther Mundwiler, Beat Mueller, Mirjam Christ-Crain, Fabian Meienberg, Fahim Ebrahimi, Christoph Henzen, Stefan Fischli, Marius Kraenzlin, Christian Meier, Philipp Schuetz
EUROPEAN JOURNAL OF ENDOCRINOLOGY
(2017)
Article
Endocrinology & Metabolism
Sandrine Andrea Urwyler, Philipp Schuetz, Fahim Ebrahimi, Marc Y. Donath, Mirjam Christ-Crain
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2017)
Article
Endocrinology & Metabolism
Fahim Ebrahimi, Sandrine A. Urwyler, Philipp Schuetz, Beat Mueller, Luca Bernasconi, Peter Neyer, Marc Y. Donath, Mirjam Christ-Crain
ENDOCRINE CONNECTIONS
(2019)
Article
Peripheral Vascular Disease
Sandrine Andrea Urwyler, Fahim Ebrahimi, Thilo Burkard, Philipp Schuetz, Marko Poglitsch, Beat Mueller, Marc Y. Donath, Mirjam Christ-Crain
Article
Endocrinology & Metabolism
Fahim Ebrahimi, Alexander Kutz, Ulrich Wagner, Ben Illigens, Timo Siepmann, Philipp Schuetz, Mirjam Christ-Crain, Beat Mueller, Emanuel R. Christ
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2020)
Article
Endocrinology & Metabolism
Milica Popovic, Fahim Ebrahimi, Sandrine Andrea Urwyler, Marc Yves Donath, Mirjam Christ-Crain
ENDOCRINE CONNECTIONS
(2020)
Editorial Material
Cardiac & Cardiovascular Systems
Guntram Schernthaner, Naim Shehadeh, Alexander S. Ametov, Anna V. Bazarova, Fahim Ebrahimi, Peter Fasching, Andrej Janez, Peter Kempler, Ilze Konrade, Nebojsa M. Lalic, Boris Mankovsky, Emil Martinka, Dario Rahelic, Cristian Serafinceanu, Jan Skrha, Tsvetalina Tankova, Zydrune Visockiene
CARDIOVASCULAR DIABETOLOGY
(2020)
Article
Endocrinology & Metabolism
Alexander Kutz, Fahim Ebrahimi, Soheila Aghlmandi, Ulrich Wagner, Miluska Bromley, Ben Illigens, Timo Siepmann, Philipp Schuetz, Beat Mueller, Mirjam Christ-Crain
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2020)
Article
Gastroenterology & Hepatology
Fahim Ebrahimi, Tracey G. Simon, Hannes Hagstrom, Jonas Soderling, Axel Wester, Bjorn Roelstraete, Jonas F. Ludvigsson
Summary: Patients with biopsy-proven NAFLD have a significantly higher risk of developing severe infections requiring hospitalization compared to the general population and their siblings. The risk of infection increases with the severity of NAFLD.
CLINICAL GASTROENTEROLOGY AND HEPATOLOGY
(2023)
Article
Medicine, General & Internal
Jiangwei Sun, Jonas Halfvarson, David Bergman, Fahim Ebrahimi, Bjorn Roelstraete, Paul Lochhead, Mingyang Song, Ola Olen, Jonas F. Ludvigsson
Summary: This study found that the use of statins is associated with a lower risk of colorectal cancer (CRC) in patients with inflammatory bowel disease (IBD). The risk reduction is more significant in patients who use statins for a longer duration. Additionally, statin users also have a decreased risk of CRC-related mortality and all-cause mortality.
Meeting Abstract
Gastroenterology & Hepatology
Axel Wester, Ying Shang, Linnea Widman, Fahim Ebrahimi
JOURNAL OF HEPATOLOGY
(2023)
Meeting Abstract
Gastroenterology & Hepatology
Fahim Ebrahimi, Tracey Simon, Hannes Hagstrom, Jiangwei Sun, David Bergman, Bjorn Roelstraete, Jonas Ludvigsson
JOURNAL OF HEPATOLOGY
(2023)
Meeting Abstract
Medicine, General & Internal
Angel Borisov, Alexander Kutz, Emanuel Christ, Tuyana Boldanova, Christine Bernsmeier, Markus Heim, Fahim Ebrahimi
SWISS MEDICAL WEEKLY
(2022)
Meeting Abstract
Gastroenterology & Hepatology
Angel Borisov, Alexander Kutz, Emanuel Christ, Tuyana Boldanova, Christine Bernsmeier, Markus Heim, Fahim Ebrahimi
JOURNAL OF HEPATOLOGY
(2021)
Article
Medicine, General & Internal
Alexander Kutz, Lara Gut, Fahim Ebrahimi, Ulrich Wagner, Philipp Schuetz, Beat Mueller
Article
Microscopy
Shengli Zhang, Ying Wang, Bowei Wang, Yi Zeng, Jiani Li, Xingyue Wang, Cuihua Hu, Zhankun Weng, Zuobin Wang
Summary: Curcumin was found to have effects on the activity, migration, and mitochondrial membrane potential of malignant hepatocytes and their mitochondria. The findings from both optical microscopy and atomic force microscopy studies confirm these effects on cell morphology and biomechanics.
Article
Microscopy
Rutika Sansaria, Krishanu Dey Das, Alwin Poulose
Summary: This paper presents a method for quantifying Golgi dispersion images and classifying them using machine learning models. By applying automated image processing and segmentation, extracting features, and using different classifiers, accurate classification of Golgi images is achieved.
Article
Microscopy
Marjorie da Silva, Cintia Eleonora Lopes Justino, Fernando Henrique Carnimeo, Fernando Barbosa Noll, Johan Billen
Summary: This article describes the secretory glands in females of two species of Myzinum and discusses their homology with other glands in the Hymenoptera order. The study finds that the three glands, instead of the commonly believed two, are present in Myzinum species. However, the limited research on the structure, function, and evolution of female accessory glands in Hymenoptera prevents conclusive statements.
Article
Microscopy
Ping Hao, Wen Yin, Xi Chen, Shuangshuang Qin, Yue Yu, Yuan Yuan, Xiaoyu Quan, Bing Hu, Shouhai Chen, Yi Wu
Summary: This study confirmed the secretion of nano-scale exosomes-multivesicular bodies and the interaction of high endothelial cells (HECs) with lysosomes and mitochondria in the small intestine of laying birds. Immunohistochemistry and immunofluorescence results showed the strong immunoreactivity of exosome-related proteins and autophagic-related proteins in the lamina propria of the small intestine. These findings reveal an innovative communication method among HECs in the small intestine.
Article
Microscopy
Stefan Talu, Robert S. Matos, Henrique Duarte da Fonseca Filho, Daniela Predoi, Simona Liliana Iconaru, Carmen Steluta Ciobanu, Liliana Ghegoiu
Summary: This study developed composite layers based on magnesium-doped hydroxyapatite and chitosan, and demonstrated their good biocompatibility and antifungal effects against osteosarcoma cells. The attachment and growth of the cells on the composite layers were promoted, while the growth of Candida albicans was inhibited. The morphological analysis revealed complex 3D patterns on the cancer cell surfaces on the magnesium-containing composite layers.