期刊
MICRON
卷 42, 期 2, 页码 196-206出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.micron.2010.08.011
关键词
AFM; Surface functionalization; Silanes; DNA; DNA supercoiling; Alternative DNA structures; Protein-DNA complexes; Single molecule imaging
类别
资金
- NIH [GM 62235]
- NSF [NSF-EPSCOR EPS-0701892, PHY-061590]
- DOE [DE-FG02-08ER64579]
- NATO [CBN.NR.NRSFP 983204]
- Nebraska Research Initiative (NRI)
Sample preparation techniques allowing reliable and reproducible imaging of DNA with various structures, topologies and complexes with proteins are reviewed. The major emphasis is given to methods utilizing chemical functionalization of mica, enabling preparation of the surfaces with required characteristics. The methods are illustrated by examples of imaging of different DNA structures. Special attention is given to the possibility of AFM to image the dynamics of DNA at the nanoscale. The capabilities of time-lapse AFM in aqueous solutions are illustrated by imaging of dynamic processes as transitions of local alternative structures (transition of DNA between H and B forms). The application of AFM to studies of protein-DNA complexes is illustrated by a few examples of imaging site-specific complexes, as well as such systems as chromatin. The time-lapse AFM studies of protein-DNA complexes including very recent advances with the use of high-speed AFM are reviewed. (C) 2010 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据