Article
Engineering, Environmental
Abdelmajeed Adam Lagum, Maria Elektorowicz
Summary: The effect of intermittent direct current on the nitrifying community structure and performance in a membrane electro-bioreactor (MEBR) was investigated. The MEBR achieved substantial nitrification rates and enhanced NH3+ -N removal compared to a conventional membrane bioreactor (MBR). Different nitrifying bacteria species dominance in the MEBR and MBR led to differences in nitrification performance. Developing a fast and robust nitrifying community in MEBR can lead to cost savings in WWTPs.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2022)
Article
Chemistry, Analytical
Somaye Cheraghi, Pelin Senel, Burcu Dogan Topal, Soykan Agar, Mahsa Majidian, Mine Yurtsever, Esen Bellur Atici, Aysegul Golcu, Sibel A. Ozkan
Summary: In this study, the interaction mechanism between Eltrombopag and DNA was investigated using voltammetry, spectroscopic techniques, and viscosity measurements. A DNA-based biosensor and nano-biosensor were developed to detect the binding of DNA and Eltrombopag. Experimental results showed that Eltrombopag strongly interacts with dsDNA, as evidenced by the reduction of dGuo and dAdo oxidation signals in the presence of the drug. UV absorption, fluorescence emission spectroscopy, and viscosity measurements were used to further characterize the binding mode of the drug. Molecular docking and molecular dynamics simulations revealed that Eltrombopag binds to DNA through intercalation.
Review
Biochemistry & Molecular Biology
Xin Ma
Summary: Mass spectrometry (MS) is a central technique used for analyzing molecular structures of unknown compounds in the gas phase. It converts molecules into ions and measures their molecular weights accurately. Tandem MS allows detailed structural characterization by breaking molecules into fragment ions. MS is widely used in multiomics studies to reveal the structures and functions of biomolecules. It is a highly sensitive and versatile technique that can be coupled with other analytical instruments. This review focuses on the technical advances of MS-based structural elucidation methods and their applications in complex mixture analysis.
Article
Environmental Sciences
Hua Peng, Kai Deng, Yu Shi, Saihua Liu, Zhu Jian, Changjun Li, Xionghui Ji, Shengnan Li
Summary: This study evaluated the effects of slag-based Si fertilizers on Cd-polluted paddy soils, soil microbial diversity, and functional properties. The results showed that Si fertilization significantly increased soil pH and decreased Cd accumulation in both the soil and rice plants. Powder form Si fertilizers exhibited the best alleviation effects. Si application increased soil microbial richness and Shannon diversity. Bacterial community composition variations were mainly explained by soil pH, NH4-N, available Cd, and Si contents.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Review
Chemistry, Physical
Zhixing Lu, Jueting Zheng, Jie Shi, Biao-Feng Zeng, Yang Yang, Wenjing Hong, Zhong-Qun Tian
Summary: Molecular electronics is a promising field that has made breakthroughs in the fabrication and application of single-molecule devices. The combination of micro/nanofabrication techniques with other methods is being actively explored, with challenges and prospects in the fabrication of single-molecule devices being discussed.
Article
Environmental Sciences
Lingwei Meng, Jichao Wang, Xiangkun Li, Yening Yu, Yining Zhu
Summary: The study utilized a lab-scale EGSB reactor to treat antibiotic wastewater and analyzed the microbial community structure in response to environmental factors changes. The results showed that the environmental factors mainly affected bacterial and fungal microbial community structure but not archaeal ones. Additionally, the relative abundance of Firmicutes was significantly affected by temperature and hydraulic retention time, leading to a decrease in COD removal efficiency.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Engineering, Environmental
Yueru Wu, Hui Liu, Wenhao Dang, MeiLing Li, Gaojin Lv, Yongli Chen, Xi Liu, Zhwei Wang
Summary: The study investigated the effect of calcification on anaerobic granular sludge, finding that calcification increased sludge density, decreased suspended solids and extracellular polymeric substances content, altered microbial community structure and microstructure, and reduced methane production and specific methanogenic activity.
JOURNAL OF WATER PROCESS ENGINEERING
(2021)
Article
Microbiology
Bin Huang, Yuxuan Chen, Zhouyang Pei, Lianqiang Jiang, Yu Zhang, Jing Wang, Jie Wang
Summary: This study investigated the effects of adding Junweinong and Junlisu microbial organic fertilizers (MOFs) after dazomet fumigation on the structure and function of crop rhizosphere microbial communities. The results showed that adding MOFs reduced nutrient levels and enzyme activities in the rhizosphere soil, but increased soil pH. Additionally, the addition of MOFs enhanced crop root nutrient uptake, microbial metabolism, and promoted the colonization of beneficial microbial communities in the rhizosphere.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Environmental Sciences
Francesco Armetta, Josue Cardenas, Eugenio Caponetti, Rosa Alduina, Alessandro Presentato, Luca Vecchioni, Pietro di Stefano, Alberto Spinella, Maria Luisa Saladino
Summary: This study conducted monitoring of environmental conditions in Santa Margherita's cave in Italy and found that degradation of ancient paintings in the cave was mainly influenced by exposure to sea aerosol, affecting salt composition and bacterial community diversity. Specific actions for conservation and restoration of the paintings and preservation of the site were suggested.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Review
Food Science & Technology
Walaa M. S. Gomaa, Xin Feng, Huihua Zhang, Xuewei Zhang, Weixian Zhang, Xiaogang Yan, Quanhui Peng, Peiqiang Yu
Summary: This review provides an update on the applications of advanced molecular spectroscopy in canola-related bio-processing technology, molecular structure, and nutrient utilization. The study focuses on how changes in molecular structure affect the nutritional quality of canola and its co-products. Various molecular spectroscopic techniques and their associations with nutrition evaluation methods are discussed in detail.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2021)
Review
Microbiology
Qingxin Li
Summary: Keratinases are proteases that degrade keratin into amino acids, playing essential roles in converting keratin-rich waste into valuable products. Despite progress in isolating keratinase-producing microorganisms and studying their structures and biochemistry, further efforts are needed to enhance the biotechnological applications of keratinase through molecular biology and protein engineering.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Fisheries
Raja Aadil Hussain Bhat, Ritesh Shantilal Tandel, Pragyan Dash, Mir Ishfaq Nazir, Dar Jaffer Yousuf, Irfan Ahmad Bhat, Parvaiz Ahmad Ganie, Pankaj Gargotra, C. Siva
Summary: In this study, the full-length TLR4 gene was cloned and characterized in Tor putitora, and its phylogenetic relationship, localization, and binding affinity with ligands were investigated. The results suggest that TpTLR4 plays an important role in sensing pathogen-associated molecular patterns, particularly of viral origin.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Engineering, Environmental
Zhi-Ling Li, Rui Cheng, Fan Chen, Xiao-Qiu Lin, Xiao-Jing Yao, Bin Liang, Cong Huang, Kai Sun, Ai-Jie Wang
Summary: The study demonstrated that sulfamethoxazole, chlortetracycline and amoxicillin have inhibitory effects on microbial denitrification performance, resulting in decreased nitrate removal efficiencies and decreased expression of important genes. Higher concentrations of antibiotics lead to stronger inhibitory effects, potentially causing changes in the abundance of denitrifying bacterial genera.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Food Science & Technology
Yang Yang, You Na Wu, Li Ge Er Ce, Xi Ge Bu Ren Ge, Quan Shuang, Feng Mei Zhang
Summary: We used high-throughput sequencing technology to investigate the structural changes in microbial flora during the fermentation process of Congee and its influence on flavor compounds. Bacterial communities in Congee samples belonged mainly to Lactobacillus, Kosakonia, and Enterococcus genera, while fungal communities were dominated by Yarrowia, Dipodascus, and Pichia genera. Gas chromatography-mass spectrometry analysis revealed important aroma compounds in Congee including ethyl acetate, eucalyptol, nonanal, heptyl ethyl acid, and acetic acid. Electronic nose technology showed that Congee samples elicited a strong response from W5S, W1S, and W1W sensors, with the strongest responses observed for Congee, sweetness, and saltiness.
Review
Physics, Multidisciplinary
R. Caciuffo, G. H. Lander, G. van der Laan
Summary: Research on actinide materials has been greatly advanced by the general techniques available from high-intensity photon beams from x-ray synchrotron sources, which can work with minute samples and reduce the radioactive hazards. The article discusses the form and encapsulation procedures used for different techniques and presents the basic theory for interpreting the results. It also reviews a selection of experiments to demonstrate the potential of synchrotron radiation techniques for studying actinide materials.
REVIEWS OF MODERN PHYSICS
(2023)
Article
Agronomy
Javier Vicente, Alejandro Alonso, Eva Navascues, Domingo Marquina, Antonio Santos
Article
Biotechnology & Applied Microbiology
Silvia Perez-Magarino, Estela Cano-Mozo, Clara Albors, Antonio Santos, Eva Navascues
Summary: The study demonstrates that the use of selected O. oeni strains in combination with adapted biomass production is an effective strategy to control histamine production in red wines. The practices over three consecutive years ensure the persistence of the selected O. oeni strain against other indigenous microbiota in the winery. Furthermore, analysis shows that low BA content is maintained during wine aging in barrels, resulting in healthier wines for consumers.
AMERICAN JOURNAL OF ENOLOGY AND VITICULTURE
(2021)
Review
Biochemistry & Molecular Biology
Javier Vicente, Fernando Calderon, Antonio Santos, Domingo Marquina, Santiago Benito
Summary: Non-Saccharomyces yeasts, particularly P. kluyveri, have shown promising contributions to wine quality improvement by enhancing aroma, glycerol, and ethanol yield. These yeasts increase volatile molecules such as esters and varietal thiols, enhancing the quality of specific varietal wines.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Food Science & Technology
Ivan Benito-Vazquez, Ignacio Belda, Javier Ruiz, Javier Vicente, Eva Navascues, Domingo Marquina, Antonio Santos
Summary: A conventional PCR method targeting B. bruxellensis was developed in this study, using a polyvinylpyrrolidone pre-treatment to allow for low detection limit of 10(2) UFC/mL in wine samples. The procedure was simplified by avoiding time-consuming DNA extraction steps, making it easy for application in wineries.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Microbiology
Javier Ruiz, Miguel de Celis, Maria Martin-Santamaria, Ivan Benito-Vazquez, Ana Pontes, Val F. Lanza, Jose Paulo Sampaio, Antonio Santos, Ignacio Belda
Summary: The widespread presence of the IRC7(S) allele in wine strains confers fitness and competitive advantages to the HS strains. The IRC7(S) allele directly contributes to improved responses to oxidative stress and higher tolerance to copper levels in the wine fermentation process. Additionally, other sequence variants may co-contribute to the overall fitness and competitive advantages observed in the HS strains.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Microbiology
Javier Vicente, Miguel de Celis, Alejandro Alonso, Domingo Marquina, Antonio Santos
Summary: Deception Island in Antarctica is a geothermal location with active fumaroles, creating a unique habitat for microbial communities. A multidisciplinary study on sediments deposited over 4 years revealed a bacterial community dominated by Proteobacteria and Thaumarchaeota, a fungal community mainly composed of Aspergillaceae, and the presence of diatoms. The study highlighted the significant influence of physicochemical conditions on microbial populations and their distribution, providing valuable data on the interaction between the island and water microbiota.
Article
Environmental Sciences
M. de Celis, L. Serrano-Aguirre, I Belda, R. Liebana-Garcia, M. Arroyo, D. Marquina, I de la Mata, A. Santos
Summary: The study indicates that the use of AHLs-degrading enzymes can reduce biofilm formation on WWTP membranes and ameliorate the virulence of Gram-negative bacteria.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Review
Food Science & Technology
Javier Vicente, Eva Navascues, Fernando Calderon, Antonio Santos, Domingo Marquina, Santiago Benito
Summary: The interest in Lachancea thermotolerans, a yeast species with unusual characteristics, has notably increased in all ecological, evolutionary, and industrial aspects. One key characteristic of L. thermotolerans is its production of high quantities of lactic acid compared to other yeast species. Its evolution has been influenced by the environment and domestication, allowing several metabolic traits to arise. The molecular regulation of the fermentative process in L. thermotolerans reveals interesting routes that play a complementary or protective role against fermentative stresses.
Article
Environmental Sciences
M. de Celis, I Belda, D. Marquina, A. Santos
Summary: This study investigated the effects of Ag and ZnO nanoparticles on a P. aeruginosa strain isolated from a wastewater treatment plant. The results showed that Ag nanoparticles had some impact on biofilm formation and iron homeostasis of the bacteria, while the higher concentrations of ZnO nanoparticles not only affected biofilm formation and iron homeostasis, but also caused cell death and increased eDNA release.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Biotechnology & Applied Microbiology
Miguel de Celis, Javier Duque, Domingo Marquina, Humbert Salvado, Susana Serrano, Lucia Arregui, Antonio Santos, Ignacio Belda
Summary: Network models and community phylogenetic analyses were used to examine the temporal dynamics of network properties in microbial communities in activated sludge systems. The results showed that during the initial assembly stages, the microbial communities adapted to the activated sludge environment, leading to an increase in network modularity and co-exclusion proportion, and a decrease in network clustering. This suggests niche specialization as a result of the selective pressure of deterministic factors in wastewater treatment plants.
NPJ BIOFILMS AND MICROBIOMES
(2022)
Article
Biotechnology & Applied Microbiology
Miguel de Celis, Javier Ruiz, Javier Vicente, Alberto Acedo, Domingo Marquina, Antonio Santos, Ignacio Belda
Summary: Wine fermentation process has a diverse population of non-Saccharomyces yeast genera, which play a crucial role in determining the sensory attributes of the resulting wines. Researchers are exploring the metabolic potential of these yeast species to enhance wine flavor and prevent spoilage.
FEMS YEAST RESEARCH
(2022)
Review
Food Science & Technology
Javier Vicente, Yasemin Baran, Eva Navascues, Antonio Santos, Fernando Calderon, Domingo Marquina, Doris Rauhut, Santiago Benito
Summary: Climate change poses problems in wine technology, such as acidity deficiency and difficulties in performing malolactic fermentation. Microbiological approaches, including increasing malic acid content and using non-Saccharomyces yeasts, provide reliable solutions. For red wine stability, malic acid removal is necessary, and alternatives to Oenococcus oeni, such as Lactiplantibacillus plantarum and Schizosaccharomyces genus, are being studied.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Javier Vicente, Javier Ruiz, Sandra Tomasi, Miguel de Celis, Candela Ruiz-de-Villa, Jordi Gombau, Nicolas Rozes, Fernando Zamora, Antonio Santos, Domingo Marquina, Ignacio Belda
Summary: The interaction between non-Saccharomyces yeasts and Saccharomyces cerevisiae affects the fermentation performance of S. cerevisiae. Among the non-Saccharomyces yeasts studied, K. unispora shows similar growth characteristics to S. cerevisiae and has a significant impact on wine fermentation.
Article
Food Science & Technology
Javier Vicente, Eva Navascues, Santiago Benito, Domingo Marquina, Antonio Santos
Summary: Climate change leads to a lack of acidity in winemaking, and oenologists are using various solutions to address this problem. Lachancea thermotolerans, a yeast that can tolerate the harsh conditions of wine, has emerged as a promising alternative for managing pH during winemaking. This study developed a manageable method for genotyping L. thermotolerans, which can be applied in wine-related laboratories for strain selection and monitoring during fermentation.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Javier Vicente, Niina Kelanne, Eva Navascues, Fernando Calderon, Antonio Santos, Domingo Marquina, Baoru Yang, Santiago Benito
Summary: The development of new fermentation strategies utilizing different wine-related species is important for adapting to new winemaking conditions and consumer preferences. The combined use of deacidifying and acidifying yeasts is a promising non-conventional approach to wine fermentation. In this study, the combination of Lachancea thermotolerans and Schizosaccharomyces pombe strains showed great performance in reducing alcohol, malic acid, and succinic acid concentrations while increasing lactic acid and ester concentrations in wine.
FERMENTATION-BASEL
(2023)