Article
Microbiology
In Young Choi, Jee-Hwan Oh, Zhiying Wang, Jan-Peter van Pijkeren
Summary: Biotherapeutic strategies, such as using engineered microbes to deliver therapeutic molecules, have great potential. However, the lack of precise tools to detect these microbes and their products hinders our ability to monitor and adjust therapeutic delivery. In this study, a bioluminescent peptide tagging system was adapted for lactic acid bacteria, which are commonly used as delivery vehicles for therapeutics. This system allowed sensitive detection of recombinant therapeutic proteins and could be used in continuous-culture bioreactor systems. Furthermore, it enabled rapid detection of administered microbes in intestinal and fecal samples.
Article
Biotechnology & Applied Microbiology
Pei Kun Richie Tay, Pei Yu Lim, Dave Siak-Wei Ow
Summary: Lactic acid bacteria can display functional proteins on their cell surface using the cell anchoring domain CAD4a, such as the antioxidant enzyme SOD, which shows potential for treating gut inflammation. CAD4a binds non-covalently to peptidoglycan in the bacterial cell wall, allowing high stability and uniform anchoring of proteins.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Review
Food Science & Technology
Haritha Meruvu, Sebnem Tellioglu Harsa
Summary: This review comprehensively describes current research on lactic acid bacteria (LAB), including their sources, growth requisites, isolation and characterization strategies, and their applications in various sectors. The review also details various industrial patents related to the isolation and identification of novel LAB strains, methods and media for detection/screening, isolation, adaptation, culturing, preservation, and growth improvement, as well as the techniques and methodologies supporting LAB fermentation and the applications of the produced industrial metabolites.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Multidisciplinary Sciences
Cecilia Rodriguez, Dema Ramlaoui, Nardin Georgeos, Briea Gasca, Camila Leal, Tomas Subils, Marisel R. Tuttobene, Rodrigo Sieira, Nicholas T. Salzameda, Robert A. Bonomo, Raul Raya, Maria Soledad Ramirez
Summary: Lacticaseibacillus rhamnosus CRL 2244 demonstrates potent inhibitory and killing activity against carbapenem-resistant Acinetobacter baumannii, leading to bacterial stress and death. The findings highlight the potential of Lcb. rhamnosus CRL 2244 as an alternative or complementary strategy for treating infections. Further exploration and development of lactic acid bacteria as a treatment option could provide valuable alternatives for combating CRAB infections.
SCIENTIFIC REPORTS
(2023)
Article
Food Science & Technology
Sneh punia Bangar, Vandana P. Chaudhary, Tejinder P. Singh, Fatih Ozogul
Summary: The increasing demand for natural products has led to the search for green and potent substitutes to prolong the shelf life of food products. Lactic acid bacteria have the potential to produce antimicrobial molecules that can both inhibit pathogens and serve as potential antibiotics. They are also effective in natural preservation methods in the food industry.
Review
Biotechnology & Applied Microbiology
Wan Abd Al Qadr Imad Wan-Mohtar, Nurul Izzah Khalid, Muhamad Hafiz Abd Rahim, Abdullah Amru Indera Luthfi, Nurul Solehah Mohd Zaini, Nur Akmal Solehah Din, Nurul Aqilah Mohd Zaini
Summary: This review discusses the potential and advantages of using underutilized Malaysian agro-industrial waste as sustainable carbon sources for lactic acid production.
FERMENTATION-BASEL
(2023)
Article
Biotechnology & Applied Microbiology
Valentina Tolu, Cristina Fraumene, Angela Carboni, Antonio Loddo, Manuela Sanna, Simonetta Fois, Tonina Roggio, Pasquale Catzeddu
Summary: This study investigated the dynamics of microbial ecosystem in three liquid sourdoughs triggered with the same microbial strains under different fermentation conditions and starter inoculation methods. The results showed a high heterogeneity of fungi microbiota and a small number of yeast species in the sourdoughs. Several bacterial species were consistently present in all sourdoughs, while Fructilactobacillus sanfranciscensis became the dominant species at the end of the propagation period.
FERMENTATION-BASEL
(2022)
Review
Food Science & Technology
Weiling Guo, Bingyong Mao, Xin Tang, Qiuxiang Zhang, Jianxin Zhao, Hao Zhang, Wei Chen, Shumao Cui
Summary: This article summarizes the common metabolites (such as SCFAs, bacteriocin, and EPS) produced by lactic acid bacteria (LAB) in fermented foods and the gut, and discusses their effects on inflammation, oxidative stress, the intestinal barrier, and gut microbiota in inflammatory bowel disease (IBD) models. The development of metabolomics technologies, such as NMR, GC-MS, and LC-MS, has contributed to the discovery of LAB-derived metabolites (LABM) and the identification of IBD biomarkers. However, further research is needed to explore undiscovered metabolites and their functions.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Agriculture, Multidisciplinary
Guowei Chen, Zixin Yang, Chunting Cao, Xue Xiao, Yadong Huang, Lingmin Tian, Weibin Bai
Summary: The study found that oral administration of the recombinant Lactococcus lactis strain pGSMT/MG1363 for 6 weeks did not have adverse effects on the physiological parameters and gut microbial compositions of rats, indicating that its stability may be considered safe compared to the non-GM strain MG1363.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2021)
Review
Microbiology
Charles Obinwanne Okoye, Yongli Wang, Lu Gao, Yanfang Wu, Xia Li, Jianzhong Sun, Jianxiong Jiang
Summary: Ensiling is a microbial-driven process used to preserve fresh forage in bio-refinery and animal production. Lactic acid bacteria (LAB) species are widely recognized as additives in silage production. However, there is a lack of information on gene expression and molecular mechanisms of microbiota involved in silage production, leading to inconsistent silage quality. Modern research focuses on improving LAB inoculants through advanced biotechnological approaches. This review highlights the role of LAB inoculants in silage production and explores the use of metabolomics, proteomics, metagenomics, genomics, transcriptomics, and genetic manipulation for developing high-performance LAB strains. It also discusses the trends and future perspectives of LAB development for silage improvement in sustainable agriculture.
MICROBIOLOGICAL RESEARCH
(2023)
Review
Microbiology
Charles Obinwanne Okoye, Yongli Wang, Lu Gao, Yanfang Wu, Xia Li, Jianzhong Sun, Jianxiong Jiang
Summary: This study discusses the role of LAB inoculants in forage production and modern biotechnology approaches such as metabolomics, proteomics, metagenomics, genomics, transcriptomics, and genetic manipulation as powerful tools for identifying, improving, and developing high-performance LAB strains. Furthermore, the review highlights the trends and future perspectives of LAB development for forage improvement, essential for breakthroughs in sustainable agriculture.
MICROBIOLOGICAL RESEARCH
(2023)
Article
Biotechnology & Applied Microbiology
Augchararat Klongklaew, Kridsada Unban, Apinun Kanpiengjai, Pairote Wongputtisin, Punnita Pamueangmun, Kalidas Shetty, Chartchai Khanongnuch
Summary: The study identified a strain, WX1, with high capability to produce optically pure l-lactic acid (l-LA) from different sugars. Optimal cultivation conditions significantly improved l-LA yield, paving the way for practical applications in bioconversion of lignocellulose, particularly glucose-xylose-rich corn stover to l-LA.
FERMENTATION-BASEL
(2021)
Article
Biotechnology & Applied Microbiology
Ernesta Augustiniene, Ilona Jonuskiene, Jurgita Kailiuviene, Edita Mazoniene, Kestutis Baltakys, Naglis Malys
Summary: This study developed a transcription factor (TF)-based whole-cell biosensor strategy for the determination of L- and D-lactic acid. The utility of biosensors as an efficient analytical tool was demonstrated, and the fermentation of lactic acid using organic-rich residual fraction (ORRF) was explored. The results showed that Lacticaseibacillus paracasei subsp. paracasei, Lactobacillus delbrueckii subsp. lactis, and Lactobacillus amylovorus have the ability to biosynthesize optically pure L-lactic acid, D-lactic acid or a mixture of both from ORRF.
MICROBIAL CELL FACTORIES
(2023)
Review
Biotechnology & Applied Microbiology
Jiapeng Wu, Yongping Xin, Jian Kong, Tingting Guo
Summary: Lactic acid bacteria are a diverse group capable of converting soluble carbohydrates into lactic acid, with applications in fermented foods and potential use as biotherapeutic vehicles. They are natural inhabitants of the human intestinal tract, providing beneficial effects on health. Advancements in genome manipulation techniques can enhance probiotic characteristics and therapeutic functionalities of LAB, maximizing their value in biotechnology.
MICROBIAL CELL FACTORIES
(2021)
Article
Biotechnology & Applied Microbiology
Gaber A. Abo-Zaid, Ahmed M. Kenawy, Nehal M. El-Deeb, Lamiaa A. Al-Madboly
Summary: In this study, the Plackett-Burman design method was used to optimize the production of the target oligosaccharide LA-EPS-20079 from Lactobacillus acidophilus. Glucose, yeast extract and sodium acetate trihydrate were found to be the top three significant variables influencing LA-EPS production. After optimization, the predicted LA-EPS-20079 concentration was 794.82 μg/ml. The optimized product showed strong cytotoxic effects against colon cancer cells and downregulated the expression levels of BCL2 and Survivin genes.
MICROBIAL CELL FACTORIES
(2023)
Article
Biotechnology & Applied Microbiology
Kim I. Sorensen, Mirjana Curic-Bawden, Mette P. Junge, Thomas Janzen, Eric Johansen
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2016)
Article
Biotechnology & Applied Microbiology
Honeylet Sabas Ochangco, Amparo Gamero, Ida M. Smith, Jeffrey E. Christensen, Lene Jespersen, Nils Arneborg
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2016)
Article
Medicine, General & Internal
Lotta K. Stenman, Markus J. Lehtinen, Nils Meland, Jeffrey E. Christensen, Nicolas Yeung, Markku T. Saarinen, Michael Courtney, Remy Burcelin, Marja-Leena Lahdeaho, Juri Linros, Dan Apter, Mika Scheinin, Hilde Kloster Smerud, Aila Rissanen, Sampo Lahtinen
Article
Gastroenterology & Hepatology
Nina Kimer, Julie S. Pedersen, Juliette Tavenier, Jeffrey E. Christensen, Troels M. Busk, Lise Hobolth, Aleksander Krag, Waleed Abu Al-Soud, Martin S. Mortensen, Soren J. Sorensen, Soren Moller, Flemming Bendtsen
JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY
(2018)
Editorial Material
Biotechnology & Applied Microbiology
Eric Johansen
MICROBIAL CELL FACTORIES
(2017)
Article
Gastroenterology & Hepatology
Puneet Puri, Suthat Liangpunsakul, Jeffrey E. Christensen, Vijay H. Shah, Patrick S. Kamath, Gregory J. Gores, Susan Walker, Megan Comerford, Barry Katz, Andrew Borst, Qigui Yu, Divya P. Kumar, Faridoddin Mirshahi, Svetlana Radaeva, Naga P. Chalasani, David W. Crabb, Arun J. Sanyal
Article
Multidisciplinary Sciences
Philippe Besnard, Jeffrey E. Christensen, Helene Brignot, Arnaud Bernard, Patricia Passilly-Degrace, Sophie Nicklaus, Jean-Paul Pais de Barros, Xavier Collet, Benjamin Lelouvier, Florence Servant, Vincent Blasco, Bruno Verges, Laurent Lagrost, Gilles Feron, Remy Burcelin
SCIENTIFIC REPORTS
(2018)
Article
Medicine, Research & Experimental
Mary Ellen Sanders, Todd R. Klaenhammer, Arthur C. Ouwehand, Bruno Pot, Eric Johansen, James T. Heimbach, Maria L. Marco, Julia Tennila, R. Paul Ross, Charles Franz, Nicolas Page, R. David Pridmore, Greg Leyer, Seppo Salminen, Duane Charbonneau, Emma Call, Irene Lenoir-Wijnkoop
Article
Biotechnology & Applied Microbiology
Yvonne Agerso, Birgitte Stuer-Lauridsen, Karin Bjerre, Michelle Geervliet Jensen, Eric Johansen, Mads Bennedsen, Elke Brockmann, Bea Nielsen
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2018)
Article
Multidisciplinary Sciences
Ida M. Smith, Adam Baker, Jeffrey E. Christensen, Teun Boekhout, Hanne Frokiær, Nils Arneborg, Lene Jespersen
Article
Multidisciplinary Sciences
Yvonne Agerso, Karin Bjerre, Elke Brockmann, Eric Johansen, Bea Nielsen, Roland Siezen, Birgitte Stuer-Lauridsen, Michiel Wels, Ahmad A. Zeidan
Correction
Cell Biology
Estelle Grasset, Anthony Puel, Julie Charpentier, Xavier Collet, Jeffrey E. Christensen, Francois Terce, Remy Burcelin
Article
Cell Biology
Estelle Grasset, Anthony Puel, Julie Charpentier, Xavier Collet, Jeffrey E. Christensen, Francois Terce, Remy Burcelin