Article
Infectious Diseases
Yue Li, Haiying Yu, Hanye Guan, Jingjing Li, Jihui Zhang, Hua Xiang, Jine Li, Huarong Tan
Summary: The improper use and abuse of antibiotics are leading to increased drug resistance, prompting the need to activate cryptic biosynthetic gene clusters from Streptomyces genomes to discover new potential drug candidates with valuable bioactivity.
Article
Biochemistry & Molecular Biology
Zhilei Li, Lang He, Xia Wang, Qingwen Huo, Guosong Zheng, Dekun Kong, Yinhua Lu, Haiyang Xia, Guoqing Niu
Summary: Sideromycins are a unique subset of siderophores that combine a siderophore and an antimicrobial agent. This study deciphered the biosynthetic pathway of the ferrichrome-type siderophore in Streptomyces sp. ATCC 700974 and identified the involved genes. The findings provide important insights into the synthesis of albomycin and the role of multiple siderophores in Streptomyces sp. ATCC 700974.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Plant Sciences
Zhiyan Wang, Feng-Xian Yang, Chongxi Liu, Li Wang, Yuxin Qi, Minghang Cao, Xiaowei Guo, Jie Li, Xueshuang Huang, Jing Yang, Sheng-Xiong Huang
Summary: Nexphenazine A, a phenazine-polyketide hybrid compound, was isolated from Streptomyces sp. KIB-H483. The biosynthesis of nexphenazine involves a gene cluster, with npzI gene playing a key role.
JOURNAL OF NATURAL PRODUCTS
(2022)
Article
Biochemistry & Molecular Biology
Wei Zhou, Hattan A. Alharbi, Eshe Hummingbird, Adrian T. Keatinge-Clay, Taifo Mahmud
Summary: This study identified that NFAT-133 is not derived from TM-123, but from a programmed extension process of a nascent heptaketide in the PKS assembly line. Mutants of the ATCC 27456 strain also revealed the presence of NFAT-133/TM-123 analogues and the involvement of NftN, NftE, and NftG in the polyketide chain assembly processes.
ACS CHEMICAL BIOLOGY
(2022)
Review
Biochemical Research Methods
Hahk-Soo Kang, Eung-Soo Kim
Summary: Heterologous expression is a key platform for linking natural product chemotypes to their genotypes. Advances in sequencing and bioinformatics have enabled rapid identification of known and cryptic BGCs from microbial genomes. Synthetic biology tools have facilitated the cloning and engineering of BGCs to enhance production yield, activating silent BGCs. Heterologous expression in Streptomyces hosts will continue to play a vital role in genomics-driven natural product research.
CURRENT OPINION IN BIOTECHNOLOGY
(2021)
Article
Microbiology
Darwin Linardi, Weiyi She, Qian Zhang, Yi Yu, Pei-Yuan Qian, Henry Lam
Summary: The study developed a high throughput proteomics-based method to complement genome mining methods for the identification of biosynthetic pathways in Streptomyces species.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Rubin Thapa Magar, Van Thuy Thi Pham, Purna Bahadur Poudel, Hue Thi Nguyen, Adzemye Fovennso Bridget, Jae Kyung Sohng
Summary: In this study, a new macrolide called 25-hydroxy peucemycin was successfully identified from Streptomyces peucetius ATCC 27,952, which exhibited antibacterial effects on certain pathogens. By inactivating a polyketide synthase gene, the biosynthetic gene cluster for the compound was identified, which is considered to be cryptic. The biosynthetic pathway of peucemycin involves five type I polyketide synthase genes encoding eight modules and utilizes unusual extender units.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Nami Lee, Mira Choi, Woori Kim, Soonkyu Hwang, Yongjae Lee, Ji Hun Kim, Gahyeon Kim, Hyeseong Kim, Suhyung Cho, Sun Chang Kim, Bernhard Palsson, Kyoung-Soon Jang, Byung-Kwan Cho
Summary: Streptomyces species have been studied for their medically important secondary metabolites, with considerable diversity between strains. This study reevaluated ten strains of Streptomyces venezuelae, revealing misclassification in seven of the strains. By mining secondary metabolite biosynthetic gene clusters and screening metabolites, a total of 59 secondary metabolites were identified.
Article
Biotechnology & Applied Microbiology
Khorshed Alam, Md. Mahmudul Islam, Saiful Islam, Jinfang Hao, Muhammad Nazeer Abbasi, Muhammad Hayat, Muhammad Shoaib, Youming Zhang, Aiying Li
Summary: Genome mining in silico approaches are used to evaluate the genomic potential of secondary bioactive chemical producers and discover new bioactive compounds in bacteria. Streptomyces, a common bacterial genus, is known for its diverse and valuable natural products with significant biological activities. Comparative genomics and genetic similarity network analysis of Streptomyces genomes revealed a large number of distinct and cryptic NP biosynthetic gene clusters, suggesting the potential for the identification of novel molecules.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Peipei Zhao, Hairong Liu, Qinghua Wu, Qingzhou Meng, Kunyu Qu, Xin Yin, Mengmeng Wang, Xiangxiang Zhao, Jun Qi, Yiwei Meng, Xuekui Xia, Lixin Zhang
Summary: Chaetomium cochliodes SD-280 is a fungus with strong antibacterial activity, and its active compound chetomin exhibits potent bioactivity against MRSA. This study shows that the addition of glutathione (GSH) effectively increases chetomin production and provides insights into the biosynthetic pathway of chetomin, identifying key enzymes involved.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Dominika Csolleiova, Renata Knirschova, Bronislava Rezuchova, Dagmar Homerova, Beatrica Sevcikova, Maria Matulova, Luz Elena Nunez, Renata Novakova, Lubomira Feckova, Rachel Javorova, Jesus Cortes, Jan Kormanec
Summary: This study developed an efficient markerless genome modification system for streptomycetes, successfully applied for insertion and deletion of BGCs, leading to increased yield and stability of the produced compounds.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Plant Sciences
Dongya Wu, Yiyu Hu, Shota Akashi, Hideaki Nojiri, Longbiao Guo, Chu-Yu Ye, Qian-Hao Zhu, Kazunori Okada, Longjiang Fan
Summary: In this study, the origin and evolution of Momilactone A biosynthetic gene clusters (MABGCs) in grass species were investigated. It was found that MABGCs originated from a MABGC-like cluster in Triticeae (BOP clade) through lateral gene transfer (LGT) and subsequent gene recruitment in the PACMAD clade of the grass lineage. The study also revealed the functional innovation of MABGCs in response to pathogen infection and allelopathy in rice and barnyardgrass.
Article
Biology
Hong Li, Wenyan Gao, Yifan Cui, Yuanyuan Pan, Gang Liu
Summary: The study successfully achieved precise control of biosynthetic gene cluster amplification through a combination of different strategies, leading to a significant increase in the production of secondary metabolites.
SCIENCE CHINA-LIFE SCIENCES
(2022)
Review
Biotechnology & Applied Microbiology
Wenfang Wang, Guosong Zheng, Yinhua Lu
Summary: Recent advances in cloning strategies of microbial natural product biosynthetic gene clusters focus on efficiently capturing BGCs, selecting and optimizing vectors for carrying large-size DNA, and methods for assembling targeted DNA fragments with appropriate vectors. The future direction aims to develop novel, universal, and high-efficiency methods for cloning NP BGCs.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Matthew M. Draelos, Anyarat Thanapipatsiri, Hilda Sucipto, Kenichi Yokoyama
Summary: Kinases in nucleoside biosynthetic pathways are found to be responsible for unexpected phosphorylation modifications, indicating a potential role beyond self-resistance. Bioinformatic analysis suggests the presence of similar modifications in other pathways, highlighting the importance of functional characterization of enzymes in nucleoside biosynthesis. Additionally, a divergent biosynthetic mechanism for antifungal nucleoside natural products was revealed, expanding our understanding of nucleoside biosynthesis.
NATURE CHEMICAL BIOLOGY
(2021)
Article
Chemistry, Applied
Lina Deng, Rui Wang, Guowei Wang, Mingxu Liu, Zhihua Liao, Guojian Liao, Min Chen
NATURAL PRODUCT RESEARCH
(2019)
Article
Polymer Science
Guojian Liao, Xixi Peng, Ting Li, Zhengyuan Ye, Xiaohong Xiang, Chen Fu
Review
Polymer Science
Yulin Yang, Guojian Liao, Chen Fu
Article
Chemistry, Multidisciplinary
Yixuan Tang, Shuang Wu, Jiaqi Lin, Liting Cheng, Jing Zhou, Jing Xie, Kexin Huang, Xiaoyou Wang, Yang Yu, Zhangbao Chen, Guojian Liao, Chong Li
Article
Biology
Linxia Liu, Guang-Jun He, Lei Chen, Jiao Zheng, Yingying Chen, Lan Shen, Xiuyun Tian, Erwei Li, Ence Yang, Guojian Liao, Linqi Wang
Article
Polymer Science
Yang Yu, Li Peng, Guojian Liao, Zhangbao Chen, Chong Li
Article
Chemistry, Multidisciplinary
Jing Xie, Qing Shen, Kexin Huang, Tingyu Zheng, Liting Cheng, Zhen Zhang, Yang Yu, Guojian Liao, Xiaoyou Wang, Chong Li
Article
Chemistry, Multidisciplinary
Yixuan Tang, Xiaoyou Wang, Jie Li, Yu Nie, Guojian Liao, Yang Yu, Chong Li
Letter
Biology
Jun Tian, Leixin Ye, Yuling Yang, Yalin Zhang, Changhua Hu, Guojian Liao
SCIENCE CHINA-LIFE SCIENCES
(2020)
Article
Chemistry, Medicinal
Chen Fu, Qian Lv, Jing Fan, Shiming Wu, Ming Lei, Xing Zhang, Xiaorong Li, Wei Zhou, Yang Yu, Wenshan Ren, Chengzhi Zhao, Guojian Liao
Summary: The synthesized polypyridyl iridium(III) complex Ir-3 exhibited potent and broad-spectrum antifungal activities against drug-resistant pathogens, low hemolytic activity towards mammalian cells, and biofilm inhibition and eradication capabilities. Additionally, Ir-3 showed low tendency to induce resistance and demonstrated potent in vivo antifungal activity in a murine model of disseminated candidiasis, suggesting its potential as a novel antifungal agent.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Chemistry, Medicinal
Xing Zhang, Minghui Wang, Xiaodi Zhu, Yan Peng, Tiwei Fu, Chang-Hua Hu, Jianfeng Cai, Guojian Liao
Summary: The emergence of drug-resistant fungal pathogens is a serious threat to vulnerable populations worldwide, and there is an urgent need for new antifungal agents. In this study, a series of lipo-gamma-AA peptides were synthesized and evaluated for their biological activities. One lead compound, MW5, showed potent and broad-spectrum antifungal activity and enhanced the effectiveness of fluconazole against resistant Candida isolates. Mechanistic investigation revealed that MW5 disrupted the cell membrane, increased reactive oxygen species production, and weakened the function of the efflux pump, resensitizing drug-resistant Candida albicans to fluconazole. Co-administration of MW5 and fluconazole also demonstrated potent in vivo antifungal activity in a murine model of mucocutaneous candidiasis. Our results suggest that lipo-gamma-AA peptides have great potential for combating drug-resistant Candida infections alone or in combination with other drugs.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Microbiology
Huimin Liu, Xiaoxia Yao, Weixin Ke, Hao Ding, Guang-Jun He, Shuang Ma, Yan Peng, Xinping Xu, Guojian Liao, Xiuyun Tian, Linqi Wang
Summary: In this study, a velvet family regulator Cva1 was identified to specifically and critically elicit early mating events, including sexual cell-cell fusion, in the human fungal pathogen Cryptococcus deneoformans. This regulation provides insight into the regulatory specificity of mating in C. deneoformans.
MICROBIOLOGY SPECTRUM
(2022)
Article
Biotechnology & Applied Microbiology
Leixin Ye, Saisai Wang, Jiao Zheng, Lei Chen, Lan Shen, Ye Kuang, Yuqiong Wang, Yan Peng, Changhua Hu, Linqi Wang, Xiuyun Tian, Guojian Liao
Summary: N-Acetylglucosamine (GlcNAc) is not only the building block of chitin but also an important signaling molecule in fungi. The catabolic pathway of GlcNAc plays a vital role in fungal biological processes. In this study, a gene cluster essential for GlcNAc utilization in Cryptococcus deneoformans, a basidiomycetous pathogen, was identified and characterized, and a novel GlcNAc kinase was discovered. These findings provide important insights into basidiomycete GlcNAc catabolism.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Review
Infectious Diseases
Youbao Zhao, Leixin Ye, Fujie Zhao, Lanyue Zhang, Zhenguo Lu, Tianxin Chu, Siyu Wang, Zhanxiang Liu, Yukai Sun, Min Chen, Guojian Liao, Chen Ding, Yingchun Xu, Wanqing Liao, Linqi Wang
Summary: The study reviews progress made in the past two decades on Cryptococcus neoformans, the highest-ranked fungal pathogen in the World Health Organization's Fungal Priority Pathogens List. It examines its global burden, clinical manifestation and management, and antifungal resistance. The purpose of the study is to drive research efforts to improve future diagnoses, therapies, and interventions associated with fungal infections.
INFECTIOUS DISEASES OF POVERTY
(2023)
Article
Immunology
Zhong Li, Bin Fu, Cathleen M. Green, Binbin Liu, Jing Zhang, Yuekun Lang, Sudha Chaturvedi, Marlene Belfort, Guojian Liao, Hongmin Li
EMERGING MICROBES & INFECTIONS
(2019)