Article
Chemistry, Inorganic & Nuclear
Diego L. Bertuzzi, Carolyne B. Braga, Gabriel Perli, Catia Ornelas
Summary: A water-soluble bifunctional dendrimer with polyamide backbone and thirty-two termini, combining advantages of ferrocenyl compounds and multifunctional dendrimer scaffold, was successfully synthesized and characterized. Cytotoxicity assays showed that the dendrimer exhibits moderate anticancer activity and selectively targets cancer cells.
EUROPEAN JOURNAL OF INORGANIC CHEMISTRY
(2022)
Article
Engineering, Biomedical
Yang Zhou, Kang Chen, Wing Kak Lin, Jinzhao Liu, Weirong Kang, Yaming Zhang, Ranyao Yang, Leigang Jin, Yiyun Cheng, Aimin Xu, Weiping Wang
Summary: This study developed a novel strategy to trigger ferroptosis by PDT and FSP1 inhibition, using a photoresponsive nanocomplex self-assembled by BODIPY-modified poly(amidoamine) (BMP) to stably encapsulate the inhibitor of FSP1 (iFSP1) and chlorin e6 (Ce6). The nanosystem demonstrated high-performance triggering of ferroptosis and immunogenic cell death (ICD) in vitro and in vivo.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Nanoscience & Nanotechnology
Michal Gorzkiewicz, Monika Marcinkowska, Maciej Studzian, Iwona Karwaciak, Lukasz Pulaski, Barbara Klajnert-Maculewicz
Summary: This study developed an innovative nanocarrier based on PAMAM dendrimer to increase the concentration of mesalazine in intestinal epithelial cells, thereby enhancing its anti-inflammatory potential. The covalent PAMAM-mesalazine conjugate was synthesized and characterized, and it was found that the drug successfully attached to the dendrimer. The use of PAMAM as a carrier improved cellular uptake and anti-inflammatory activity of mesalazine.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2023)
Article
Pharmacology & Pharmacy
Evgeny K. Apartsin, Nadezhda Knauer, Ulf Dietrich Kahlert, Anne-Marie Caminade
Summary: Dendritic molecules with metal complexes are potential therapeutic entities, and metallodendrons are particularly of interest as antitumor agents. In this study, we synthesized amphiphilic metallodendrons and tested their antitumor activity against glioblastoma. The results showed that the metallodendrons exhibited higher cytotoxic activity compared to the clinical standard of care for glioblastoma, indicating their potential as antitumor entities.
Article
Chemistry, Multidisciplinary
Zhiming Cheng, Emily J. Thompson, Lorena Mendive-Tapia, Jamie Scott, Sam Benson, Takanori Kitamura, Ana Senan-Salinas, Youhani Samarakoon, Edward W. Roberts, Maykel A. Arias, Julian Pardo, Eva M. Galvez, Marc Vendrell
Summary: Cytotoxic immune cells, including T lymphocytes (CTLs) and natural killer (NK) cells, play a crucial role in host response against tumors. This study presents the rational design of near-infrared fluorogenic substrates for human GzmA and mouse GzmA. These probes show high catalytic efficiency and selectivity in detecting GzmA in tissue lysates. Moreover, the probes enable real-time imaging of adaptive immune cell response to antigen-driven recognition of cancer cells.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Editorial Material
Biochemical Research Methods
Nina Vogt
Summary: DeepLabCut-Live! performs real-time pose estimation and enables closed-loop feedback on behavior.
Article
Pharmacology & Pharmacy
Serah Kimani, Suparna Chakraborty, Ikponmwosa Irene, Jo de la Mare, Adrienne Edkins, Andre du Toit, Ben Loos, Angelique Blanckenberg, Annick Van Niekerk, Leticia Costa-Lotufo, K. N. ArulJothi, Selwyn Mapolie, Sharon Prince
Summary: The study investigated the anti-cancer activity of a palladium complex, BTC2, in human breast cancer cells, demonstrating its selectivity, inhibition of cancer cell migration, and suppression of cancer stem cell activity. BTC2 also induced DNA double strand breaks, triggered cell cycle arrests, and activated multiple cell death pathways, showing promising anti-breast cancer activity both in vitro and in vivo.
BIOCHEMICAL PHARMACOLOGY
(2021)
Article
Biophysics
A. K. M. Arifuzzman, Norh Asmare, Tevhide Ozkaya-Ahmadov, Ozgun Civelekoglu, Ningquan Wang, A. Fatih Sarioglu
Summary: Characterization and identification of cell populations using surface markers are crucial in various applications. However, conventional techniques are limited by the need for immunolabeling, expensive instruments, and skilled operators. This study introduces an autonomous microchip that can electronically quantify the immunophenotypical composition of a cell suspension. The microchip captures different cell subtypes in separate microfluidic chambers and electrically monitors all on-chip activity, allowing real-time determination of subpopulation fractions. The capability to analyze immune cells without labels has the potential to enable remote testing and quality control in cell manufacturing.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Cell Biology
Ineke D. C. Jansen, Thijs van Velzen, Teun J. de Vries, Robert Szulcek, Jack J. W. A. van Loon
Summary: In this study, an impedimetric bioassay was introduced for real-time monitoring of osteoclast resorption. The method involves culturing osteoclasts on a calcium phosphate substrate with electrodes and applying different chemical stimuli to measure their activity continuously. The results showed that inflammatory factors and bisphosphonates significantly affected osteoclastic activity. This method is of great importance for studying osteoclast function and diseases, as well as for high-throughput drug testing.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Chemistry, Inorganic & Nuclear
Nedunchezhian Kavitha, Vijayan Thamilarasan, Nallathambi Sengottuvelan
Summary: Three newly synthesized ferrocene-appended cyclometalated iridium(III) complexes showed promising antimicrobial activities against various bacteria and fungi, with complexes 1 and 2 exhibiting strong bactericidal and fungicidal effects. In addition, complex 1 displayed the highest cytotoxicity against HepG2 cancer cells among all the synthesized complexes.
JOURNAL OF ORGANOMETALLIC CHEMISTRY
(2021)
Article
Chemistry, Inorganic & Nuclear
Alice Johnson, Linda Iffland, Kuldip Singh, Ulf-Peter Apfel, Kogularamanan Suntharalingam
Summary: A novel silver(I) polymeric complex bearing a dithiacyclam ligand was reported to have potent activity against cancer stem cells (CSCs), inducing CSC death through cytosol entry, increased intracellular reactive oxygen species, and caspase-dependent apoptosis. This complex demonstrated similar efficacy to salinomycin in both monolayer and three-dimensional culture models.
DALTON TRANSACTIONS
(2021)
Article
Biochemistry & Molecular Biology
Rashmi Kumar, Candace C. C. Liu, Sean C. Bendall, Michael Angelo
Summary: This study introduces a synthesis method of a dendrimer-based polymer and its application in MIBI-TOF tissue imaging. By comparing the staining performance of antibodies conjugated with dendrimers or linear polymers in FFPE tissue, it was found that dendrimers and linear polymers have comparable avidities. Moreover, dendrimers have advantages such as less background staining and no off-target binding in neural tissue. Overall, this research provides a versatile framework for using third-generation dendrimer-conjugated antibodies with improved staining compared to conventional linear polymers.
Article
Engineering, Biomedical
Dorsa Haji Ghaffari, Akwasi Darkwah Akwaboah, Ehsan Mirzakhalili, James D. Weiland
Summary: Retinal prostheses aim to improve visual perception in patients with photoreceptor degeneration-induced blindness, but the current limitations in shape and letter perception are due to low spatial resolution. Research has shown that better patient outcomes are correlated with spatially separate phosphenes. By using an optimization algorithm to find optimal stimulation parameters for focal RGC activation, the time required for optimizing implant operation can be reduced.
IEEE TRANSACTIONS ON NEURAL SYSTEMS AND REHABILITATION ENGINEERING
(2021)
Article
Pharmacology & Pharmacy
Uttom Nandi, Ichioma Onyesom, Dennis Douroumis
Summary: The study investigated the effect of sirolimus encapsulated liposomes on cancer therapy, finding that factors such as incubation time and lipid composition influenced their antiproliferative effect. Both conventional and Stealth liposomes demonstrated antiproliferative effects against prostate cancer cells, with the nanoparticles being internalized in the cytoplasm. Further development of sirolimus liposomes for targeted drug delivery to cancer sites using specific antibodies showed promising results.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2021)
Review
Chemistry, Medicinal
Li Li, Lin Ma, Jian Sun
Summary: Ferrocene derivatives show potential activity against drug-resistant cancers and are useful for developing novel anticancer candidates. Their preclinical trials demonstrate promising results and offer insights for further rational design.
CURRENT TOPICS IN MEDICINAL CHEMISTRY
(2021)
Article
Materials Science, Biomaterials
Mehmet Senel, Fatma Ebru Koc
INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS
(2020)
Article
Biochemistry & Molecular Biology
Mehmet Senel, Muamer Dervisevic, Lars Esser, Esma Dervisevic, JenniSfer Dyson, Christopher D. Easton, Victor J. Cadarso, Nicolas H. Voelcker
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2020)
Article
Chemistry, Analytical
Mehmet Senel, Muamer Dervisevic, Sammy Alhassen, Amal Alachkar, Nicolas H. Voelcker
ANALYTICAL CHEMISTRY
(2020)
Article
Chemistry, Physical
Emre Cevik, Huseyin Tombuloglu, Ismail Anil, Mehmet Senel, Hussein Sabit, Sayed AbdulAzeez, J. Francis Borgio, Mohammad Barghouthi
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2020)
Article
Chemistry, Analytical
Mehmet Senel, Esma Dervisevic, Sammy Alhassen, Muamer Dervisevic, Amal Alachkar, Victor J. Cadarso, Nicolas H. Voelcker
ANALYTICAL CHEMISTRY
(2020)
Article
Biotechnology & Applied Microbiology
Shaimaa Abdel-Ghany, Sara Raslan, Huseyin Tombuloglu, Aly Shamseddin, Emre Cevik, Osama A. Said, Engy F. Madyan, Mehmet Senel, Ayhan Bozkurt, Suriya Rehman, Hussein Sabit
Article
Chemistry, Multidisciplinary
Muamer Dervisevic, Maria Alba, Li Yan, Mehmet Senel, Thomas R. Gengenbach, Beatriz Prieto-Simon, Nicolas H. Voelcker
Summary: The microneedle array-based transdermal sensing system offers a pain-free alternative for continuous glucose monitoring of interstitial fluid. The system demonstrates good selectivity and sensitivity, with accurate correlation to blood glucose levels measured by a commercial glucometer.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Chemistry, Analytical
Mehmet Senel, Zehra Durmus, Amal Alachkar
Summary: The constructed clozapine sensor using reduced graphene oxide nanocomposites showed good linear range, detection limit, reproducibility, and reusability. Experimental results demonstrated a linear dynamic range of 0.05-10 μM clozapine with an actual detection limit of 50 nM under optimal experimental parameters.
Article
Biochemistry & Molecular Biology
Sammy Alhassen, Mehmet Senel, Amal Alachkar
Summary: In this study, we found that L-DOPA forms a stable complex with Scn in the presence of Fe3+ using surface plasmon resonance technique. The affinity constant (Kd) of the DOPA:Fe3+ binding with Scn was lower than L-DOPA plasma peak concentrations, suggesting a significant role for the L-DOPA-Scn complex in the decreased bioavailability of L-DOPA with the progression of PD.
ACS CHEMICAL NEUROSCIENCE
(2022)
Article
Chemistry, Multidisciplinary
Amal Alachkar, Sammy Alhassan, Mehmet Senel
Summary: In this study, a simple, robust, and stable microfluidic sensor was developed for real-time measurement of dopamine in cerebrospinal fluid from mice, with the advantages of low cost and small volume.
Article
Chemistry, Physical
T. Sagir, M. Huysal, M. Senel, S. Isik, N. Burgucu, O. Tabakoglu, M. Zaim
Summary: Novel folate-receptor-targeted polyamidoamine (PAMAM) dendrimer functional mesoporous silica-coated magnetic nanoparticles were prepared for drug delivery in photodynamic therapy. The nanoparticles showed efficient photodynamic therapy effect on MCF-7 cells, causing apoptosis.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Ender Sarigul, Merve Zaim, Mehmet Senel, Tugba Sagir, Sevim Isik
Summary: A novel drug carrier was designed with an interior for drug encapsulation and a biocompatible surface, showing inhibitory effects on the proliferation of gastric adenocarcinoma cells.
Article
Chemistry, Analytical
Mehmet Senel
Summary: With the increasing number of patients suffering from Parkinson's disease, the measurement of drug levels in patient body fluids, particularly for clozapine, has become significantly important. There is a growing demand for a cost-effective platform that can analyze biofluids in real-time and in small volumes. This study aims to measure the level of clozapine using a portable potentiostat and a practical approach. The designed sensor showed good performance in terms of linearity, detection limit, reproducibility, and reusability. It was able to detect clozapine in real samples within a wide range of concentrations.
Article
Engineering, Biomedical
Eliz Selmin Paker, Mehmet Senel
Summary: In this study, PEI-grafted chitosan was successfully prepared for the fabrication of layer-by-layer films, and methylene blue was used as a model drug to investigate the drug loading and release behavior of the films. The results showed that the loading capacity and release rate of the model drug were influenced by ionic strength and pH, and PEI-grafted chitosan was demonstrated to be a suitable material for layer-by-layer films in drug delivery applications.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2022)
Article
Biochemical Research Methods
Mehmet Senel, Amal Alachkar
Summary: A novel lab-in-a-pencil graphite microfluidic sensing electrode (mu FSE) was fabricated for real-time flow injection measurement of the antipsychotic drug clozapine (Clz), using a simple, low-cost, and reusable method. The mu FSE has tubular geometry with 800 mu m diameter, providing fast and effective Clz detection with good sensitivity and detection limit.
