Article
Ophthalmology
Shereen H. Chew, Cassandra Martinez, Kathleen R. Chirco, Sangeetha Kandoi, Deepak A. Lamba
Summary: The purpose of this study was to accelerate and synchronize the differentiation of photoreceptors in retinal organoids by inhibiting the Notch signaling pathway at different developmental stages using the small molecule PF-03084014. The results showed that treating the organoids with PF resulted in changes in the expression of progenitor and photoreceptor markers, with an increase in differentiated photoreceptor markers and a decrease in progenitor markers. The findings suggest that timing the inhibition of the Notch pathway can enrich the population of early cone or rod photoreceptors in human retinal organoids.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2022)
Article
Multidisciplinary Sciences
Xuan Zheng, Max A. Betjes, Pascal Ender, Yvonne J. Goos, Guizela Huelsz-Prince, Hans Clevers, Jeroen S. van Zon, Sander J. Tans
Summary: This study developed a new tool called TypeTracker, which identifies cell fates by AI-enabled cell tracking and propagates end point fates back along the branched lineage trees. The research found that cells commit to their new type early, when still deep inside the crypt, contrary to the conventional belief that cells differentiate as they move up the crypt-villus axis. This study raises new questions about the underlying commitment and sorting mechanisms.
Review
Biochemistry & Molecular Biology
Han Leng Ng, Elizabeth Quail, Mark N. Cruickshank, Daniela Ulgiati
Summary: Notch signaling plays a critical role in cellular development, particularly in the embryogenesis and differentiation of various progenitor cell types. By regulating cell fate in hematopoiesis, Notch influences the fate of lymphoid cells. Investigating the bi-directional and lateral Notch signaling pathways may offer novel therapeutic options for Notch-mediated diseases.
Review
Genetics & Heredity
Xin Zhang, Patrick Leavey, Haley Appel, Neoklis Makrides, Seth Blackshaw
Summary: This review provides insights into the recent advances in understanding the molecular mechanisms controlling neurogenesis and specification of the developing retina, with a focus on comparative single cell multiomic analysis. It discusses the mechanisms by which extrinsic factors trigger transcriptional changes and the core evolutionarily conserved gene regulatory networks that influence retinal progenitor cells and neurogenesis.
TRENDS IN GENETICS
(2023)
Article
Neurosciences
Hriday Shanker Bindu, Hriday Shanker Pandey, Pankaj Seth
Summary: This study investigates the role of ZIKV non-structural proteins NS4A and NS4B in altering the fate of fetal neural stem cells (fNSCs). The study found that these proteins can affect cell proliferation, induce premature neurogenesis, and dysregulate notch signaling through autophagy. The study suggests that autophagy could be a potential target for therapeutic intervention against ZIKV.
MOLECULAR NEUROBIOLOGY
(2023)
Review
Cell Biology
Satish Kumar Tiwari, Sudip Mandal
Summary: Over the years, Drosophila has been a valuable genetic model system to study tissue-resident stem cells, with recent advancements in understanding the contribution of mitochondria to governing stem cell properties.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Mathematical & Computational Biology
Dasong Huang, Ruiqi Wang
Summary: The endocrine and exocrine cells in the pancreas originate from apparently identical endoderm cells in the early gut. Notch signaling is critical in determining the fate of endocrine and exocrine cells in the developing pancreas, functioning as a typical mediator of lateral inhibition. High (or low) levels of Notch signaling drive cells to select the fate of exocrine (or endocrine) progenitor cells.
MATHEMATICAL BIOSCIENCES AND ENGINEERING
(2021)
Article
Cell Biology
Jiadong Zheng, Yifan Guo, Changyi Shi, Shuai Yang, Wenyan Xu, Xianjue Ma
Summary: This study reveals the important role of Ire1 in cell competition, regulating the fate of loser cells through controlling apoptosis and autophagy, and provides molecular insights into scrib-mediated TSCC.
Article
Biochemistry & Molecular Biology
Otto Morris, Heinrich Jasper
Summary: Reactive Oxygen Species (ROS) play critical signaling roles in the intestine, maintaining the regenerative capacity of Intestinal Stem Cells (ISCs). Declines in metabolic adaptations, through aging or pathology, result in reciprocal changes in ISC metabolism and ROS signaling, ultimately leading to aberrant ISC function and loss of tissue homeostasis.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Multidisciplinary Sciences
Xiaochan Xu, Philip Allan Seymour, Kim Sneppen, Ala Trusina, Anuska la Rosa Egeskov-Madsen, Mette Christine Jorgensen, Mogens Hogh Jensen, Palle Serup
Summary: Through mathematical modeling, it is found that the Notch signaling pathway plays a crucial role in pancreatic cell fate choice. The two Notch ligands, Jag1 and Dll1, guide the differentiation of multipotent pancreatic progenitor cells into unipotent pro-acinar cells and bipotent duct/endocrine progenitors by different mechanisms. The cell-autonomous attenuation of Notch signaling mediated by Jag1 is important for cell differentiation.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Qingzhe Wu, Jingxin Guo, Yuning Liu, Qi Zheng, Xiaoling Li, Chuanqiang Wu, Dong Fang, Xin Chen, Liang Ma, Pinglong Xu, Xiaofang Xu, Cheng Liao, Ming Wu, Li Shen, Hai Song
Summary: The study reveals that YAP plays critical roles in the establishment of intratumoral heterogeneity in SCLC, affecting the development of SCLC by promoting fate conversion from NE to Non-NE tumor cells and enhancing chemoresistance.
Article
Developmental Biology
Yukino Wada, Hikaru Tsukatani, Chihiro Kuroda, Yurika Miyazaki, Miku Otoshi, Isao Kobayashi
Summary: This study identifies a previously unknown spatiotemporal regulatory mechanism of hematopoietic stem cell specification by somites in zebrafish, in which Jag2b-induced Notch signaling leads to the expression of wnt16 and HSC markers.
Article
Multidisciplinary Sciences
Yan Ting Shue, Alexandros P. Drainas, Nancy Yanzhe Li, Sarah M. Pearsall, Derrick Morgan, Nasa Sinnott-Armstrong, Susan Q. Hipkins, Garry L. Coles, Jing Shan Lim, Anthony E. Oro, Kathryn L. Simpson, Caroline Dive, Julien Sage
Summary: This study identifies REST and YAP as key components of the Notch signaling pathway in controlling neuroendocrine fate in the lungs. They demonstrate the importance of REST and YAP in regulating neuroendocrine to non-neuroendocrine cell fate switches in lung development, lung injury response, and small-cell lung cancer.
NATURE COMMUNICATIONS
(2022)
Article
Developmental Biology
Sumitra Tatapudy, Jobelle Peralta, Todd Nystul
Summary: In Drosophila ovarian epithelial follicle stem cells, the E2 ubiquitin ligase, Bendless, plays multiple roles in JNK signaling, differentiation, proliferation, and niche competition, with a relationship to hedgehog signaling pathway regulation.
Article
Cell Biology
Covadonga F. Hevia, Carolyn Engel-Pizcueta, Frederic Udina, Cristina Pujades
Summary: This study investigates the cellular and molecular mechanisms that regulate the balance between progenitor cell proliferation and neuronal differentiation in the embryonic brain construction. By using a CRISPR-based knockin zebrafish transgenic line, the researchers generate a detailed lineage of hindbrain boundary cells. They find that these boundary cells undergo a functional transition from neuroepithelial progenitors to radial glia cells, coincide with the activation of Notch3 signaling, and subsequently undergo asymmetric cell division. Loss of Notch3 function leads to symmetric cell division and neuronal differentiation. The fate of boundary cells is to become neurons, with the subtype determined by their axial position.
