期刊
MEAT SCIENCE
卷 86, 期 2, 页码 436-439出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.meatsci.2010.05.030
关键词
Longissimus; Water holding capacity; Reference genes; Real-time quantitative PCR; Calpastatin; RPL4
资金
- Irish National Development Plan
The aim of this study was to identify a set of stably expressed endogenous control genes for quantitative PCR analysis of mRNA expression in the porcine LTL muscle and to subsequently perform expression analysis of potential candidate genes associated with drip loss. Expression stability of seven commonly used reference genes was examined in n=60 pigs from three independent populations of different genetic backgrounds. The genes examined were: ACTB, ATP5G1, B2M, GPX1, RPL4, TBP and YWHAZ. GeNorm analysis of expression stability identified B2M. RPL4 and TBP as consistently stable in each breed examined. Analysis of meat samples divergent for water holding capacity identified positive and negative associations between drip loss and gene expression using B2M, RPL4 and TBP as endogenous controls. Specifically, expression of COL1A1 increased significantly with increasing drip loss while expression of CAST decreased significantly with increasing drip loss. This study therefore indicates the use of B2M, RPL4 and TBP as suitable endogenous controls for gene expression analysis of the porcine LTL muscle. Further study is recommended to identify the detailed roles of COL1A1 and CAST with respect to the development of drip loss. (C) 2010 The American Meat Science Association. Published by Elsevier Ltd. All rights reserved.
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