期刊
MACROMOLECULAR BIOSCIENCE
卷 15, 期 3, 页码 395-404出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/mabi.201400352
关键词
chitosan; DC-targeting; nanoparticles; phage display; vaccine delivery system
资金
- Animal Disease Management Technology Development, Ministry of Agriculture, Food and Rural Affairs, Republic of Korea [313014-03]
The paper presents a novel dendritic cells (DC)-targeting peptide, TPAFRYS (TP) identified by phage display technique and conjugated to chitosan in order to develop an efficient DC-targeting vaccine delivery carrier. TP-conjugated chitosan nanoparticles (TPC-NPs) were prepared with ovalbumin (OVA) as a model vaccine by ionic gelation. Flow cytometry and immunocytochemistry studies demonstrated the higher targeting ability of TPC-NPs to DCs in compared to chitosan NPs. Moreover, TPC-NPs exhibited higher targeting specificity in DCs than macrophage and myoblasts. Furthermore, immunization of mice with OVA-loaded TPC-NPs enhanced OVA-specific serum IgG and IgG isotype antibodies production. Thus, DC-targeting strategy demonstrates a potential approach to enhance the effectiveness of vaccines.
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