Article
Polymer Science
Chia-Hsin Ko, Cristiane Henschel, Geethu P. Meledam, Martin A. Schroer, Peter Mueller-Buschbaum, Andre Laschewsky, Christine M. Papadakis
Summary: The temperature-dependent phase behavior and self-assembly behavior of a thermoresponsive amphiphilic diblock copolymer in aqueous solution were studied, revealing a lower critical solution temperature (LCST) behavior at 31.1 degrees Celsius and the formation of different structures of micelles.
Article
Polymer Science
Migle Babelyte, Laura Peciulyte, Vesta Navikaite-Snipaitiene, Joana Bendoraitiene, Volodymyr Samaryk, Ramune Rutkaite
Summary: Thermoresponsive chitosan-graft-poly(N-isopropylacrylamide) copolymers with different compositions were synthesized. The chemical structure of the copolymers was confirmed and their lower critical solution temperature (LCST) behavior was assessed. Copolymers with a certain content of poly(N-isopropylacrylamide) side chains demonstrated LCST behavior and could potentially be used in drug delivery systems or tissue engineering.
Article
Materials Science, Multidisciplinary
Evgenii A. Ksendzov, Pavel A. Nikishau, Irina M. Zurina, Viktoria S. Presniakova, Peter Timashev, Yuri A. Rochev, Svetlana Kotova, Sergei Kostjuk
Summary: A series of well-defined macromonomers and copolymers were synthesized via ROP and RAFT-mediated copolymerization, respectively. The cloud point temperature and transition temperature of the graft copolymers showed dependence on the copolymer composition. Films prepared from these copolymers displayed better cell adhesion compared to neat PNIPAM.
ACS APPLIED POLYMER MATERIALS
(2022)
Article
Polymer Science
Chia-Hsin Ko, Cristiane Henschel, Geethu P. Meledam, Martin A. Schroer, Renjun Guo, Luka Gaetani, Peter Mueller-Buschbaum, Andre Laschewsky, Christine M. Papadakis
Summary: The self-assembly of the thermoresponsive amphiphilic diblock copolymer PMMA(21)-b-PNIPAM(283) in various water/methanol mixtures was studied. It was found that the solvent composition significantly affected the internal morphology of micelles and the conformation of dissolved chains.
Article
Polymer Science
Xiangming Fu, Yanqiu Wang, Liang Xu, Atsushi Narumi, Shin-ichiro Sato, Xiaoran Yang, Xiande Shen, Toyoji Kakuchi
Summary: Poly(N,N-bis(2-ethoxyethyl)acrylamide) (PEOEAm), a new thermoresponsive polyacrylamide, was studied in terms of synthesis and thermal phase transition. PEOEAm without initiator residue at the chain end was synthesized via hydrosilylation-promoted group transfer polymerization (GTP) to exclude the effect of polymer end groups on its thermoresponsive properties. By copolymerizing PEOEAm and DMAm, various block copolymer architectures were synthesized and their thermoresponsive properties were evaluated.
Article
Biophysics
Alberto Guerron, Huu Trong Phan, Carolina Penaloza-Arias, Davide Brambilla, Valerie Gaelle Roullin, Suzanne Giasson
Summary: The attachment/detachment of cells on responsive polymer-coated surfaces is influenced by various factors including electrostatic interactions, wettability and swelling of the polymer coating. In this study, dual thermo- and pH-responsive cationic poly(N-isopropylacrylamide) microgels, surface-functionalized with PEG or PDMAEMA, were used as substrates to investigate their impact on cell behavior. The results showed that microgels with PEG chains had comparable cell adhesion and proliferation rates to controls, while exhibiting the best thermo-triggered cell detachment performance, especially for MCF7 and U138 cells. The hydrophilic nature of the microgel surface remained unchanged regardless of temperature and pH, contributing to this behavior. Swelling behavior influenced by pH resulted in significant cell detachment only for U138 cells. However, detachment of bEnd.3 cells could not be triggered by either temperature or pH, regardless of the microgel surface coating.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Polymer Science
Shin-nosuke Nishimura, Kei Nishida, Tomoya Ueda, Shohei Shiomoto, Masaru Tanaka
Summary: Poly(N-isopropylacrylamide) (PNIPAM) is a commonly used thermoresponsive polymer that has been widely applied in biological applications. However, due to the presence of a hydrogen-bond donor, PNIPAM often induces blood clot formation, making alternatives to PNIPAM with aprotic polar functional groups necessary. In this study, acrylate polymers containing the aprotic polar functional group pyrrolidone ring were synthesized, and their lower critical solution temperatures (LCSTs) could be precisely controlled. These polymers demonstrated good blood compatibility and cell affinity, and interestingly, could activate macrophages without cytotoxicity when their hydrophobic/hydrophilic balance was altered.
Article
Chemistry, Physical
Fang Yin, Barbara Lonetti, Jean-Daniel Marty, Nancy Lauth-de Viguerie
Summary: The properties of thermosensitive polymers can be easily altered using carbon dioxide (CO2) as a trigger. Copolymers of N,N-(diethylamino)ethyl acrylamide (DEAEAM) and N-isopropylacrylamide (NIPAM) were synthesized via reversible addition-fragmentation chain transfer (RAFT) polymerization, resulting in different compositions and architectures. The properties of these polymers in aqueous solution in the presence of CO2 were studied, and it was observed that both composition and architecture play a role in determining the thermoresponsive behavior.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2023)
Article
Polymer Science
Katharina Nieswandt, Prokopios Georgopanos, Martin Held, Evgeni Sperling, Volker Abetz
Summary: Thermoresponsive copolymers were synthesized and their thermoresponsive properties were characterized. The copolymers showed a lower critical solution temperature in water, which depended on the molar fraction of one of the monomers. Block copolymers were also prepared using a surfactant-free emulsion polymerization method, and their thermoresponsive behavior in a confined state was investigated. The nanospheres showed a linear change in hydrodynamic radius with temperature, and the addition of certain salt anions affected the collapse of the nanospheres' corona.
Article
Polymer Science
Alexander Plucinski, Marko Pavlovic, Mairi Clarke, David Bhella, Bernhard V. K. J. Schmidt
Summary: The study investigated the aggregation behavior of high molar mass block copolymer in an organic solvent, revealing unprecedented behavior under specific conditions. Potential future applications in photonics and templating of inorganic structures were envisioned for this new thermo-responsive block copolymer.
MACROMOLECULAR RAPID COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Elena Yu. Kozhunova, Anna V. Plutalova, Andrey V. Sybachin, Alexander V. Chertovich, Elena V. Chernikova
Summary: This study investigated the double stimuli-responsive properties of PNIPA and PVIM block copolymers in aqueous solutions for the first time. The synthesis of two different block copolymers was performed using RAFT polymerization. The conformational behavior and self-assembly ability of the polymers were found to depend on the location of hydrophobic groups derived from the RAFT agent within the chain, as well as the length and charge of the PVIM block.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Mariia Levit, Alena Vdovchenko, Apollinariia Dzhuzha, Natalia Zashikhina, Elena Katernyuk, Alexey Gostev, Eugene Sivtsov, Antonina Lavrentieva, Tatiana Tennikova, Evgenia Korzhikova-Vlakh
Summary: Novel bio-inspired amphiphilic block-copolymers were synthesized and self-assembled into nanoparticles of approximately 200 nm in aqueous media, demonstrating enhanced thermal stability, low cytotoxicity, and retained biological activity against lung adenocarcinoma epithelial cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Polymer Science
Tingyu He, Yanqiu Wang, Atsushi Narumi, Liang Xu, Shin-ichiro Sato, Xiande Shen, Toyoji Kakuchi
Summary: This paper presents a comprehensive study on the thermoresponsive properties of statistic copolymers and multiblock copolymers synthesized with different copolymerization methods. It was found that the disorder of copolymers plays a decisive role in phase-transition behavior, with some copolymers exhibiting a two-stage phase transition in water.
Article
Chemistry, Physical
Clemence Nadal, Stephane Gineste, Olivier Coutelier, Audrey Tourrette, Jean-Daniel Marty, Mathias Destarac
Summary: Block copolymers based on poly(vinylamine) and poly(N-isopropylacrylamide) were synthesized and their thermoresponsive and aggregation behavior in aqueous solution were studied. The copolymers showed self-assembling properties at room temperature and tended to form larger aggregates upon heating.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2022)
Article
Chemistry, Multidisciplinary
Yujun Cheng, Qilong Mao, Chunxiang Zhou, Xuexiang Huang, Jiabin Liu, Jiawei Deng, Zhe Sun, Seonghun Jeong, Yongjoon Cho, Youhui Zhang, Bin Huang, Feiyan Wu, Changduk Yang, Lie Chen
Summary: SCOSCs based on conjugated block copolymers show improved efficiency and stability by covalently bonding a polymer donor and polymer acceptor. The precise manipulation of sequence length and distribution regularity of CBCs optimizes film morphology, improves optoelectronic properties, and reduces energy loss. The D18(40)-b-PYIT-based device achieves a high efficiency of 13.4% with enhanced stability, and blade-coated large-area SCOSCs deliver an impressive efficiency of 11.62%/10.73%.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Biochemistry & Molecular Biology
Minami Matsuura, Mariko Ohshima, Yuki Hiruta, Tomohiro Nishimura, Kenichi Nagase, Hideko Kanazawa
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2018)
Article
Biochemical Research Methods
Kohei Okubo, Koji Ikeda, Ayaka Oaku, Yuki Hiruta, Kenichi Nagase, Hideko Kanazawa
JOURNAL OF CHROMATOGRAPHY A
(2018)
Article
Biochemistry & Molecular Biology
Kenichi Nagase, Momoko Hasegawa, Eri Ayano, Yoshie Maitani, Hideko Kanazawa
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2019)
Article
Biochemistry & Molecular Biology
Jiatu Li, Taisei Kaku, Yuki Tokura, Ko Matsukawa, Kenta Homma, Taihei Nishimoto, Yuki Hiruta, Aya Mizutani Akimoto, Kenichi Nagase, Hideko Kanazawa, Seimei Shiratori
Article
Chemistry, Multidisciplinary
Minami Maekawa-Matsuura, Kei Fujieda, Yutaro Maekawa, Tomohiro Nishimura, Kenichi Nagase, Hideko Kanazawa
Article
Chemistry, Analytical
Kenichi Nagase, Maria Watanabe, Fumihiko Zen, Hideko Kanazawa
ANALYTICA CHIMICA ACTA
(2019)
Article
Chemistry, Analytical
Kenichi Nagase, Sakiko Kitazawa, Sota Yamada, Aya Mizutani Akimoto, Hideko Kanazawa
ANALYTICA CHIMICA ACTA
(2020)
Article
Biophysics
Kenichi Nagase, Naho Uchikawa, Tadashi Hirotani, Aya Mizutani Akimoto, Hideko Kanazawa
COLLOIDS AND SURFACES B-BIOINTERFACES
(2020)
Article
Biochemistry & Molecular Biology
Kento Takaya, Ayano Sunohara, Noriko Aramaki-Hattori, Shigeki Sakai, Keisuke Okabe, Hideko Kanazawa, Toru Asou, Kazuo Kishi
Summary: This study analyzed the expression and localization of Wnt proteins in a mouse fetal-wound-healing model and found their association with scar formation. The results indicated that Wnt signaling may be involved in wound healing and scar formation. Regulation of Wnt signaling could be a possible mechanism to control scar formation.
Article
Biophysics
Kenichi Nagase, Akari Okada, Junnosuke Matsuda, Daiju Ichikawa, Yutaka Hattori, Hideko Kanazawa
Summary: In this study, a thermoresponsive cationic block copolymer brush-grafted beads were developed as packing materials for ADSC separation columns. These columns can adsorb ADSCs at 37 degrees C and elute them at 4 degrees C, while other cells are not adsorbed. This separation column can be used to isolate ADSCs without cell surface modification, while maintaining cell activity.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Engineering, Biomedical
Kenichi Nagase, Kaichi Yamazaki, Yutaro Maekawa, Hideko Kanazawa
Summary: Temperature-modulated exosome-capturing materials were developed using thermoresponsive polymers and peptides with affinity for exosomes. The copolymer-grafted silica beads successfully captured and released targeted exosomes via temperature modulation.
MATERIALS TODAY BIO
(2023)
Article
Materials Science, Biomaterials
Kenichi Nagase, Naoto Kojima, Mitsuaki Goto, Toshihiro Akaike, Hideko Kanazawa
Summary: In this study, a hepatocyte cell separation material was developed using a thermoresponsive polymer and a polymer with high affinity to hepatocytes. The temperature modulation allowed for the effective separation of hepatocytes from other cells. This material holds significance for cell therapy and liver tissue engineering.
JOURNAL OF MATERIALS CHEMISTRY B
(2022)
Article
Materials Science, Biomaterials
Kenichi Nagase, Goro Edatsune, Yuki Nagata, Junnosuke Matsuda, Daiju Ichikawa, Sota Yamada, Yutaka Hattori, Hideko Kanazawa
Summary: A temperature-modulated mesenchymal stem cell separation column was developed in this study, allowing for the adsorption and elution of MSCs through temperature changes. The column successfully purified MSCs while retaining cellular activity, demonstrating its usefulness for MSC separation without cell modification.
BIOMATERIALS SCIENCE
(2021)
Article
Materials Science, Biomaterials
Kenichi Nagase, Masaki Shimura, Ruka Shimane, Kengo Hanaya, Sota Yamada, Aya Mizutani Akimoto, Takeshi Sugai, Hideko Kanazawa
Summary: A novel cell separation method using a thermoresponsive block copolymer brush with an affinity peptide was developed in this study, allowing for selective endothelial cell adhesion and detachment without cell modification. The method showed specificity in cell separation and could be conducted using a simple procedure, making it a promising tool for cell purification in tissue engineering and cell transplantation therapy.
BIOMATERIALS SCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Taihei Nishimoto, Ko Matsukawa, Kenichi Nagase, Hideko Kanazawa, Aya Mizutani Akimoto, Ryo Yoshida
CHEMICAL COMMUNICATIONS
(2020)
