期刊
MACROMOLECULAR BIOSCIENCE
卷 12, 期 5, 页码 628-636出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/mabi.201100404
关键词
linear poly(ethyleneimine); mammalian cells; molecular weight distribution; recombinant proteins; transient gene expression
资金
- Swiss Innovation Promotion Agency KTI/CTI [10563.1]
Three synthesis lots of linear poly(ethyleneimine) (PEI) are compared to a fully hydrolyzed linear PEI (commercially available as PEI Max) regarding structure, polyplex formation with plasmid DNA, and transfection of suspension-adapted HEK-293E cells. PEI Max binds DNA more efficiently than the other PEIs, but it is the least effective in terms of transient recombinant protein yield. One PEI lot is fractionated by means of SEC. The fractions of high-$\overline {M} _{{\rm n}} $ PEI are the most efficient for complex formation and transfection. Nevertheless, the highest transient recombinant protein yields are achieved with unfractionated PEI. The results demonstrate that the polydispersity and charge density of linear PEI are important parameters for gene delivery to suspension-adapted HEK-293E cells.
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