期刊
MACROMOLECULAR BIOSCIENCE
卷 12, 期 1, 页码 75-82出版社
WILEY-BLACKWELL
DOI: 10.1002/mabi.201100274
关键词
gene delivery; nanocomplexes; recombinant proteins; spider silk; tumor-homing peptides
资金
- NIH [EB002520]
- NSF (DK)
- JSPS
- National Science Foundation [ECS-0335765]
- Grants-in-Aid for Scientific Research [23750264] Funding Source: KAKEN
Nanoscale complexes of recombinant silk molecules containing THPs with DNA are designed as less cytotoxic and highly target-specific gene carriers. Genetically engineered silk proteins containing poly(L-lysine) domains to interact with pDNA and the THP to bind to specific tumorigenic cells for target-specific pDNA delivery are prepared, followed by in vitro transfection into MDA-MB-435 melanoma cells, highly metastatic human breast tumor MDA-MB-231?cells, and non-tumorigenic MCF-10A breast epithelial cells. The silk/poly(L-lysine) block copolymer containing Lyp1 (ML-Lyp1) shows significant differences from silk/poly(L-lysine) block copolymer containing F3 (ML-F3) in cytotoxicity to MCF10A cells. ML-F3 is the most promising candidate for target delivery into tumorigenic cells.
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