期刊
MACROMOLECULAR BIOSCIENCE
卷 10, 期 7, 页码 731-735出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/mabi.201000075
关键词
separation techniques; ultracentrifugation
资金
- Biomolecolor Interactions Technology Center (BITC) at the University of New Hampshire, Durham, NH, USA
The ability to obtain a sedimentation coefficient distribution as the run proceeds, and to get an early idea of the quality of a particular sample, has not been made available in real-time during the run in any of the existing software packages. It is desirable on many occasions to be able to see the number of components present in a sample at an early stage of the run. The ability to ascertain the extent of heterogeneity of sample would help enormously to reduce the amount of time that is necessary to obtain that information. Most software packages currently available require that the run be completed before analysis is carried out or at least some of the early scans analyzed off-line to determine if the run should continue. A software package called SEDVIEW has been developed by us to allow early analysis in real-time.
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