期刊
MACROMOLECULAR BIOSCIENCE
卷 11, 期 3, 页码 344-351出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/mabi.201000305
关键词
drug delivery systems; graft copolymers; radicals; reactive oxygen species; siRNA
资金
- Ministry of Education, Culture, Sports, Science, and Technology of Japan (MEXT) [21240050]
- Japan Science and Technology Agency
- Grants-in-Aid for Scientific Research [22659008, 21240050, 22800004] Funding Source: KAKEN
A novel oligonucleotide carrier which can scavenge ROS is described. The synthesized graft polymer is composed of a PEG segment and a TEMPO-containing hydrophobic segment for scavenging ROS. This graft polymer can form a PIC through electrostatic interaction with oligonucleotides such as siRNA. The amount of ROS was monitored by fluorescence measurements using H2DCFDA as a probe, and it was confirmed that the ROS level was effectively suppressed. The cellular uptake of PIC containing the fluorescence-labeled oligonucleotide was evaluated by fluorescence microscopy. Delivered siRNA suppressed the expression of the mRNA. The prepared graft copolymer is thus a promising candidate as a novel oligonucleotide carrier which also reduces ROS damage generated by cationic polymer carriers.
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