Article
Materials Science, Multidisciplinary
A. Logeshwaran, Chaitra Kabadi Srinivas, V. Venkatesh, Sunita Nayak
Summary: 3D printing technology is a convincing method for fabricating scaffolds for tissue replacement. In this study, polymer-ceramic composite 3D-printed scaffolds with silk fibroin infill were investigated for their cell adhesion. The presence of silk fibroin improved cell adhesion and viability, but the mechanical strength remained similar.
Article
Materials Science, Biomaterials
Na Sun, Jiajing Wang, Xiaoqiu Dou, Yiqi Wang, Yuan Yang, Dong Xiao, Peiquan Zhao, Jing Li, Shuting Wang, Ping Gu, Jing Ji
Summary: Retinal progenitor cell transplantation is a potential strategy for retinal degeneration, and the chiral features of the extracellular microenvironment are crucial for cell proliferation. This study used l/d-phenylalanine-derived molecules to construct a biomimetic chiral microenvironment, which effectively enhanced the proliferation of retinal progenitor cells.
BIOMATERIALS SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Sarah Grossi, Annalisa Grimaldi, Terenzio Congiu, Arianna Parnigoni, Giampiero Campanelli, Paola Campomenosi
Summary: The study compared the growth of human primary fibroblasts on different biological matrices, revealing differences in attachment and proliferation abilities, as well as activation of gene expression programs. Variations were observed in the expression of metalloproteinases and interleukin-6 among different 3D matrices.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Nanoscience & Nanotechnology
Mahdiyeh Nouri-Goushki, Livia Angeloni, Khashayar Modaresifar, Michelle Minneboo, Pouyan E. Boukany, Mohammad J. Mirzaali, Murali K. Ghatkesar, Lidy E. Fratila-Apachitei, Amir A. Zadpoor
Summary: A systematic study was conducted to investigate the effects of surface topography on cell adhesion and function, revealing that the geometry and arrangement of submicron pillars have a significant impact on cell adhesion, formation of focal adhesions, and cell morphology. Cells interacting with tall and dense pillars exhibited enhanced adhesion parameters and formation of focal adhesions, as well as specific cell settlement patterns and cytoskeletal organization. Additionally, tall and dense submicron pillars upregulated the expression of osteopontin and promoted matrix mineralization.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Chemistry, Physical
Asad Masood, Naeem Ahmed, Mf Mohd Razip Wee, Muhammad Asm Haniff, Ebrahim Mahmoudi, Anuttam Patra, Kim S. Siow
Summary: Pulsed plasma polymerisation was used to produce an antibacterial coating from carvone oil, and the coating's properties could be adjusted based on deposition parameters. The coatings with predominantly C-O and C=O showed smaller static water contact angles, stronger bactericidal effects, and smoother and more homogeneous surfaces.
SURFACE INNOVATIONS
(2022)
Article
Biochemistry & Molecular Biology
Olegs Borodins, Felix Broghammer, Michael Seifert, Nils Cordes
Summary: Cancer is a highly heterogeneous disease with (epi)genetic characteristics that determine resistance to therapy. Preclinical research has identified the adhesome as a critical resistance mechanism in multiple cancers. This study connects preclinical data with transcriptomic and patient survival data, revealing a set of overexpressed genes in different cancers that negatively affect patient survival, particularly in radiotherapy-treated cohorts. These genes, including key integrins and their interconnectors, may serve as therapeutic targets in cancer treatment.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Yanqun Wang, Jie Li, Lu Zhang, Hai-Xi Sun, Zhaoyong Zhang, Jinjin Xu, Yonghao Xu, Yu Lin, Airu Zhu, Yuxue Luo, Haibo Zhou, Yan Wu, Shanwen Lin, Yuzhe Sun, Fei Xiao, Ruiying Chen, Liyan Wen, Wei Chen, Fang Li, Rijing Ou, Yanjun Zhang, Tingyou Kuo, Yuming Li, Lingguo Li, Jing Sun, Kun Sun, Zhen Zhuang, Haorong Lu, Zhao Chen, Guoqiang Mai, Jianfen Zhuo, Puyi Qian, Jiayu Chen, Huanming Yang, Jian Wang, Xun Xu, Nanshan Zhong, Jingxian Zhao, Junhua Li, Jincun Zhao, Xin Jin
Summary: This study compared cfRNA profiles between COVID-19 patients and healthy donors, and found 380 up-regulated cfRNA molecules in all COVID-19 patients. Several potential biomarkers were identified. Antiviral and neutrophil activation-related genes showed decreased expression levels during treatment, which is consistent with enhanced inflammatory response in COVID-19 patients. Differential expression of noncoding RNAs and changes in tRNA pools related to SARS-CoV-2 replication were also observed. Additionally, pneumonia-related microorganisms were detected in the plasma of COVID-19 patients.
Article
Biotechnology & Applied Microbiology
Xue Li, Xin Guo, Yuqing Zhu, Guoqing Wei, Yanlei Zhang, Xia Li, Huijun Xu, Jiazhen Cui, Wenjun Wu, Jingsong He, Matthew E. Ritchie, Taylor M. Weiskittel, Hu Li, Hua Yu, Lijuan Ding, Mi Shao, Qian Luo, Xiaoxiao Xu, Xinyi Teng, Alex H. Chang, Jin Zhang, He Huang, Yongxian Hu
Summary: This study longitudinally profiled the transcriptomes of T cells in a PCL patient treated with BCMA CAR-T cells and identified distinct expression patterns in CAR-T and endogenous T cells at different stages, with CAR-T cells transitioning from a highly proliferative state to a highly cytotoxic state at peak phase. The findings were further confirmed through re-analysis of a CD8+ CD19 CAR-T single cell study, suggesting conserved mechanisms for CAR-T treatment across hematological malignancies.
Article
Biology
Hyun Ji Kim, Matthew J. Rames, Florian Goncalves, C. Ward Kirschbaum, Breeshey Roskams-Hieter, Elias Spiliotopoulos, Josephine Briand, Aaron Doe, Joseph Estabrook, Josiah T. Wagner, Emek Demir, Gordon Mills, Thuy T. M. Ngo
Summary: EVs play a critical role in the transport of extracellular small RNA, but little is known about the carriers of cf-mRNA in human biofluids and their association with cancer. In this study, the researchers analyzed the transcriptome of plasma from lung cancer, liver cancer, multiple myeloma, and healthy donors. They found that the majority of cf-mRNA was enriched in EVs, which showed remarkable stability in RNase-rich environments. The specific enrichment patterns of cancer-associated cf-mRNA in each plasma fraction were observed, suggesting the potential of EV subpopulations as a liquid biopsy approach.
COMMUNICATIONS BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Anna Majkowska, Karla E. Inostroza-Brito, Mariel Gonzalez, Carlos Redondo-Gomez, Alistair Rice, Jose Carlos Rodriguez-Cabello, Armando E. Del Rio Hernandez, Alvaro Mata
Summary: The study demonstrates the possibility to generate coassembling tubular membranes with distinct structures, properties, and bioactivity by minor modifications in the molecular structures of peptide amphiphiles and elastin-like recombinamers.
Article
Materials Science, Multidisciplinary
Xiaojing Zhu, Can Zhang
Summary: In this study, a micropatterned nanowire film was fabricated on titanium surface to manipulate cell behavior and achieve spatially specific alignment of osteoblasts. This research will benefit cell function adjustment on titanium surface.
MATERIALS TODAY COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Fumitoshi Irie, Yuki Tobisawa, Ayako Murao, Hayato Yamamoto, Chikara Ohyama, Yu Yamaguchi
Summary: The extracellular matrix (ECM) and its component hyaluronan (HA) play crucial roles in tumor cell invasion and migration dynamics. TMEM2 is identified as a key player in HA degradation and cell-substrate adhesion, shedding light on the mechanisms of cell migration in an HA-rich environment.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Letter
Oncology
Eileen M. Boyle, Adam Rosenthal, Hussein Ghamlouch, Yan Wang, Phillip Farmer, Michael Rutherford, Cody Ashby, Michael Bauer, Sarah K. Johnson, Christopher P. Wardell, Yubao Wang, Antje Hoering, Carolina Schinke, Sharmilan Thanendrarajan, Maurizio Zangari, Bart Barlogie, Madhav V. Dhodapkar, Faith E. Davies, Gareth J. Morgan, Frits van Rhee, Brian A. Walker
Summary: Sequencing studies have revealed differences in gene expression between smouldering multiple myeloma (SMM) and symptomatic multiple myeloma (MM), with shared dysregulated genes highlighting the importance of the PRC2.1 complex. Subgroup-specific differences exist even at the SMM stage of disease, suggesting distinct biological features drive progression within each TC molecular subgroup. The data suggest that MMSET SMM has already transformed, while other precursor diseases are distinct clinical entities from their symptomatic counterpart.
Article
Chemistry, Multidisciplinary
Yingying Zhou, Xianfeng Ping, Yusi Guo, Boon Chin Heng, Yijun Wang, Yanze Meng, Shengjie Jiang, Yan Wei, Binbin Lai, Xuehui Zhang, Xuliang Deng
Summary: A Mesenchymal stem cell Differentiation Prediction (MeD-P) framework is reported for predicting biomaterial-induced cell lineage fate. MeD-P integrates public RNA-seq data related to tri-lineage differentiation of human mesenchymal stem cells (hMSCs) and uses a predictive model based on the k-nearest neighbors (kNN) strategy. MeD-P achieves an overall accuracy of 90.63% on testing datasets, significantly higher than the model based on canonical marker genes (80.21%). Furthermore, MeD-P accurately predicts lineage fate on different types of biomaterials as early as the first week of hMSCs culture. In conclusion, MeD-P is an efficient and accurate strategy for stem cell lineage fate prediction and preliminary biomaterial functional evaluation.
ADVANCED MATERIALS
(2023)
Article
Cell Biology
Alia Hadefi, Morgane Leprovots, Max Thulliez, Orianne Bastin, Anne Lefort, Frederick Libert, Antoine Nonclercq, Alain Delchambre, Francois Reniers, Jacques Deviere, Marie-Isabelle Garcia
Summary: The study showed that CAP treatment has differential toxic effects on normal and tumor cells, with normal organoids being more sensitive to CAP treatment while tumor organoids exhibit higher resistance. This pilot study suggests that the ex vivo culture system could be a relevant alternative model to further investigate translational medical applications of CAP technology.
CELL DEATH DISCOVERY
(2022)
