Article
Agriculture, Multidisciplinary
Jin-xing Song, Meng-xiang Wang, Yi-xuan Zhang, Bo Wan, Yong-kun Du, Guo-qing Zhuang, Zi-bin Li, Song-lin Qiao, Rui Geng, Ya-nan Wu, Gai-ping Zhang
Summary: This study generated an ASFV antibody phage-display library, screened a specific single-chain antibody against ASFV major capsid protein, and identified a conserved epitope peptide that can promote the maturation of immune cells and be effectively uptaken by dendritic cells.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2023)
Article
Biochemical Research Methods
Wen-Ching Lin, Hao-Cheng Tang, Han Ying Wang, Chia-Yi Kao, You-Chiun Chang, Athena Hsu Li, Shi-Bing Yang, Kurt Yun Mou
Summary: This study introduces a unique mutagenesis method called the reverse Kunkel method, which combines the advantages of user-defined mutagenesis and random mutagenesis. By coupling with phage display and yeast display selections, the method successfully generates improved antibodies with enhanced affinity and immunostaining performance.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Qianlin Li, Fuqiang Zhang, Yi Lu, Huan Hu, Jin Wang, Cheng Guo, Qiang Deng, Conghui Liao, Qin Wu, Tingsong Hu, Zeliang Chen, Jiahai Lu
Summary: In this study, we developed potent multivalent VHH antibodies targeting the E2 glycoprotein of CHIKV, with Nb-2E8 and Nb-3C5 showing high-affinity binding within the nanomolar range.
JOURNAL OF NANOBIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Keisuke Fukunaga, Yohei Yokobayashi
Summary: In this study, a novel library-vs-library in vitro selection strategy was developed to select synthetic and orthogonal RNA-RBP pairs. The selected pairs exhibited picomolar affinity and >4000-fold selectivity.
NUCLEIC ACIDS RESEARCH
(2022)
Review
Chemistry, Organic
Beatriz de Pina Mariz, Sara Carvalho, Iris L. Batalha, Ana Sofia Pina
Summary: Enzymes are protein catalysts widely used in natural and industrial processes, with the global enzymes market projected to reach $10.5 billion in 2024. The development of artificial enzymes, created through computational design and directed evolution, aims to expand catalytic power and create novel catalysts with promising activity. The combination of these technologies is opening new opportunities in pharmaceutical, chemical, biofuels, and food industries, contributing to a more sustainable development.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Linnea Charlotta Hjelm, Hanna Lindberg, Stefan Stahl, John Lofblom
Summary: Affibody molecules are small affinity proteins with excellent properties. A new type of protein scaffold based on a dimeric form of affibodies has been developed, which has a distinct secondary structure content and mode of binding. The scaffold forms a tunnel-like cavity upon binding, encapsulating the target peptide. Selections from a high-complexity phage-displayed library using this scaffold resulted in the identification of high-affinity binders that effectively inhibit amyloid aggregation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Virology
Wanzhi Huang, Victoria Soeung, David M. Boragine, Liya Hu, B. V. Venkataram Prasad, Mary K. Estes, Robert L. Atmar, Timothy Palzkill
Summary: This study utilized a high-resolution method to map multiple antibody binding sites simultaneously from complex serum samples. The results indicated that a relatively small number of sites on the virus bind a large number of independently generated antibodies, suggesting that immunodominance plays a role in the humoral immune response to NoV infections.
JOURNAL OF VIROLOGY
(2021)
Review
Immunology
Diya Hu, Aaron T. Irving
Summary: This article introduces the activation mechanism of adaptive immunity following viral infections and focuses on the importance of studying and mapping epitopes. It also discusses the principles, advantages, and disadvantages of the latest epitope mapping techniques, using viral antigen discovery as examples.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Chemistry, Analytical
Sandra Oloketuyi, Robert Bernedo, Andreas Christmann, Justyna Borkowska, Giulia Cazzaniga, Horst Wilhelm Schuchmann, Joanna Niedziolka-Joensson, Katarzyna Szot-Karpinska, Harald Kolmar, Ario de Marco
Summary: C-reactive protein (CRP) is an inflammation biomarker that should be accurately quantified during infections and healing processes. Nanobodies show promise as alternatives to conventional antibodies in immunodiagnostics, with the ability to achieve higher binder density on capture surfaces. By comparing two different selection strategies, researchers were able to isolate anti-CRP nanobodies with low nanomolar affinity, suitable for use in ELISA and immunoprecipitation assays. One of the nanobodies was fused to SpyTag and used in an electrochemical biosensor to detect CRP with high sensitivity.
Article
Biochemistry & Molecular Biology
Karl W. Barber, Ellen Shrock, Stephen J. Elledge
Summary: CRISPR-inspired systems have been developed for genome editing and nucleic acid detection. The CRISPR-based peptide display technology introduced in this study allows for customized, high-throughput in vitro protein interaction studies by self-assembling bespoke peptide libraries on a DNA microarray surface for rapid, multiplexed binding assays. This platform shows promise for viral epitope mapping and multiplexed diagnostics.
Article
Biochemistry & Molecular Biology
Jing Yi Lai, Angela Corona, Chong Lee Ng, Enzo Tramontano, Yee Siew Choong, Theam Soon Lim
Summary: Ebola virus is notorious for causing severe and deadly haemorrhagic fever. Effective diagnosis and treatment are needed due to the high fatality rate. Two monoclonal antibodies have been approved for EVD treatment. The viral surface glycoprotein is a common target, but VP35 could be a potential target as well.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Ailin Zhang, HuiJuan Zhao, Shaohan Pei, Yue Chi, Xiuhua Fan, Jianqing Liu
Summary: This study identified the key allergenic epitopes of cashew nut proteins, Ana o 2 and Ana o 3, and found that Ana o 3 may be more allergenic than Ana o 2.
Article
Virology
Yu-Ming Gong, Xue-Feng Wei, Yu-Ying Zheng, Yang Li, Qing Yu, Peng-Fei Li, Bin Zhu
Summary: This study identified an antigen epitope, Pep3, that provided protection against TiLV in fish. The study also identified the protective epitope S1(399-410) and demonstrated its effectiveness in neutralizing TiLV. This research provides a new approach for screening antigen epitopes of emerging viral diseases.
JOURNAL OF VIROLOGY
(2023)
Article
Medicine, Research & Experimental
Mahmoud Ganji, Pooria Safarzadeh Kozani, Fatemeh Rahbarizadeh
Summary: Monoclonal antibody-based immunotherapies have shown promising results in treating immunological and oncological conditions. CD19 is a qualified antigen for B-cell neoplasms treatment, and VHHs have advantages over conventional mAbs as potential therapeutics and diagnostic tools. By isolating CD19-specific VHHs from a novel immune library using phage display, it was demonstrated that selected VHHs exhibited high specificity, sensitivity, and affinity to CD19, making them potential targeting tools for CD19-based immunotherapeutics.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Yunier Serrano-Rivero, Julieta Salazar-Uribe, Marcela Rubio-Carrasquilla, Frank Camacho-Casanova, Oliberto Sanchez-Ramos, Alain Gonzalez-Pose, Ernesto Moreno
Summary: This study successfully generated anti-EGF nanobodies from a synthetic library, using a selection strategy involving four different elution steps and three rounds of selection. The results demonstrate the feasibility of selecting nanobodies against small antigens like EGF from synthetic libraries.
Article
Biochemistry & Molecular Biology
Gertrudis Rojas, Tania Carmenate, Kalet Leon
JOURNAL OF MOLECULAR RECOGNITION
(2015)
Review
Oncology
Kalet Leon, Karina Garcia-Martinez, Tania Carmenate, Gertrudis Rojas
SEMINARS IN ONCOLOGY
(2018)
Article
Medicine, Research & Experimental
Gertrudis Rojas, Yanelys Cabrera Infante, Amaury Pupo, Tania Carmenate
Article
Medicine, Research & Experimental
Yaima Tundidor, Claudia Patricia Garcia-Hernandez, Amaury Pupo, Yanelys Cabrera Infante, Gertrudis Rojas
Article
Medicine, Research & Experimental
Yanelys Cabrera Infante, Amaury Pupo, Gertrudis Rojas
Article
Multidisciplinary Sciences
Kaare Bjerregaard-Andersen, Hedda Johannesen, Noha Abdel-Rahman, Julie Elisabeth Heggelund, Helene Mykland Hoas, Fana Abraha, Paula A. Bousquet, Lene Stokken Hoydahl, Daniel Burschowsky, Gertrudis Rojas, Stefan Oscarson, Geir Age Loset, Ute Krengel
SCIENTIFIC REPORTS
(2018)
Article
Multidisciplinary Sciences
Gertrudis Rojas, Tania Carmenate, Julio Felipe Santo-Tomas, Pedro A. Valiente, Marlies Becker, Annia Perez-Riveron, Yaima Tundidor, Yaquelin Ortiz, Jorge Fernandez de Cossio-Diaz, Luis Graca, Stefan Duebl, Kalet Leon
SCIENTIFIC REPORTS
(2019)
Article
Multidisciplinary Sciences
Yaima Tundidor, Luis F. Ponce, Lisset Chao, Joaquin Solozabal, Michael Hust, Stefan Duebel, Gertrudis Rojas
SCIENTIFIC REPORTS
(2020)
Article
Multidisciplinary Sciences
Janin Korn, Dorina Schaeckermann, Toni Kirmann, Federico Bertoglio, Stephan Steinke, Janyn Heisig, Maximilian Ruschig, Gertrudis Rojas, Nora Langreder, Esther Veronika Wenzel, Kristian Daniel Ralph Roth, Marlies Becker, Doris Meier, Joop van den Heuvel, Michael Hust, Stefan Duebel, Maren Schubert
SCIENTIFIC REPORTS
(2020)
Article
Biochemistry & Molecular Biology
Yury Valdes-Balbin, Darielys Santana-Mederos, Lauren Quintero, Sonsire Fernandez, Laura Rodriguez, Belinda Sanchez Ramirez, Rocmira Perez-Nicado, Claudia Acosta, Yanira Mendez, Manuel G. Ricardo, Tays Hernandez, Gretchen Bergado, Franciscary Pi, Annet Valdes, Tania Carmenate, Ubel Ramirez, Reinaldo Oliva, Jean-Pierre Soubal, Raine Garrido, Felix Cardoso, Mario Landys, Humberto Gonzalez, Mildrey Farinas, Juliet Enriquez, Enrique Noa, Anamary Suarez, Cheng Fang, Luis A. Espinosa, Yassel Ramos, Luis Javier Gonzalez, Yanet Climent, Gertrudis Rojas, Ernesto Relova-Hernandez, Yanelys Cabrera Infante, Sum Lai Losada, Tammy Boggiano, Eduardo Ojito, Kalet Leon, Fabrizio Chiodo, Francoise Paquet, Guang-Wu Chen, Daniel G. Rivera, Dagmar Garcia-Rivera, Vicente Verez Bencomo
Summary: Research shows that macromolecular constructs with recombinant RBD conjugated to tetanus toxoid can induce a potent immune response in laboratory animals, demonstrating the potential of these conjugate COVID-19 vaccine candidates.
ACS CHEMICAL BIOLOGY
(2021)
Article
Biochemical Research Methods
Luis Ariel Espinosa, Yassel Ramos, Ivan Andujar, Enso Onill Torres, Gleysin Cabrera, Alejandro Martin, Diamile Roche, Glay Chinea, Monica Becquet, Isabel Gonzalez, Camila Canaan-Haden, Elias Nelson, Gertrudis Rojas, Beatriz Perez-Masson, Dayana Perez-Martinez, Tamy Boggiano, Julio Palacio, Sum Lai Lozada Chang, Lourdes Hernandez, Kathya Rashida de la Luz Hernandez, Saloheimo Markku, Marika Vitikainen, Yury Valdes-Balbin, Darielys Santana-Medero, Daniel G. Rivera, Vicente Verez-Bencomo, Mark Emalfarb, Ronen Tchelet, Gerardo Guillen, Miladys Limonta, Eulogio Pimentel, Marta Ayala, Vladimir Besada, Luis Javier Gonzalez
Summary: Subunit vaccines based on the receptor-binding domain (RBD) of the spike protein of SARS-CoV-2 are a promising strategy to fight the COVID-19 pandemic. Mass spectrometry analysis of the protein structure is necessary, and a non-conventional digestion protocol can improve sequence coverage and detect hydrophilic regions.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)