Article
Food Science & Technology
Adam Staniszewski, Monika Kordowska-Wiater
Summary: One approach to maintaining a healthy microbiota in the human gastrointestinal tract is through the consumption of probiotics. This study examined the probiotic potential of yeast strains isolated from Polish wines. The tested strains belonged to six different species and were evaluated for their probiotic properties, safety, enzymatic activity, and antioxidant properties. The results showed that certain strains of H. uvarum, M. pulcherrima, and S. cerevisiae exhibited promising probiotic characteristics.
Article
Biotechnology & Applied Microbiology
Pedro Pais, Jorge Oliveira, Vanda Almeida, Melike Yilmaz, Pedro T. Monteiro, Miguel C. Teixeira
Summary: The study found that S. cerevisiae var. boulardii exhibited higher survival capability and acetate biosynthesis in an intestinal-like medium compared to S. cerevisiae, possibly related to its probiotic activity. Analysis of a genomic database for S. cerevisiae var. boulardii suggested a potential link between variations in transcription factor binding sites and transcriptomic patterns.
Review
Food Science & Technology
Adam Staniszewski, Monika Kordowska-Wiater
Summary: The paper discusses the concept of probiotic yeasts and their application in functional foods, emphasizing their positive impact on health, and pointing out future research directions.
Article
Biotechnology & Applied Microbiology
Jang Eun Lee, Eunjung Lee
Summary: This study aimed to discover yeast strains with intestinal anti-inflammatory activities by exhibiting probiotic properties in a DSS-induced colitis mice model. The S. cerevisiae 28-7 (SC28-7) strain was selected as a potential probiotic yeast for treating and preventing intestinal inflammatory diseases. Results showed that SC28-7 administration attenuated colon damage and inflammation, indicating its potential therapeutic value.
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Review
Food Science & Technology
Fereshteh Ansari, Shohre Alian Samakkhah, Ali Bahadori, Seyedeh Maedeh Jafari, Mojtaba Ziaee, Mohammad Taghi Khodayari, Hadi Pourjafar
Summary: Saccharomyces cerevisiae var. boulardii (S. boulardii) has been found in dairy products and possesses various beneficial activities such as anticarcinogenic, antibacterial, antiviral, and antioxidant properties. It can be used as a probiotic microorganism or starter culture in fermented dairy foods, with potential health advantages and therapeutic functions, particularly in preventing and treating gastrointestinal infectious diseases.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Biotechnology & Applied Microbiology
Ran Li, Xing Wan, Timo M. Takala, Per E. J. Saris
Summary: By cloning the gene encoding the antilisterial peptide leucocin C from a lactic acid bacterium into Saccharomyces boulardii, a strain was created that secreted a peptide capable of inhibiting Listeria monocytogenes. This modified S. boulardii demonstrated efficient killing of L. monocytogenes without the need for antibiotic selection pressure, suggesting its potential as a carrier for therapeutics delivery.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2021)
Article
Agriculture, Dairy & Animal Science
Nathalie Le Floc'h, Caroline Stephanie Achard, Francis Amann Eugenio, Emmanuelle Apper, Sylvie Combes, Helene Quesnel
Summary: Feeding sows with live yeast Saccharomyces cerevisiae var. boulardii (SB) can influence the fecal microbiota of sows and their piglets, but does not have an effect on their health and performance.
JOURNAL OF ANIMAL SCIENCE
(2022)
Article
Biochemical Research Methods
Suryang Kwak, Bejan Mahmud, Gautam Dantas
Summary: This study presents a expandable and tunable transactivation system, validated in probiotic yeast Saccharomyces boulardii, allowing for precise control of functionality through the introduction of new target sequences. The system enhances orthogonality of transactivation through complementarity between promoter and scRNA, as well as binding specificity between scRNA and transcriptional activator. Additionally, control of transactivation is achieved via inducible promoter for activator expression, showing potential for precise therapeutic functions in engineered probiotic yeast.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Jungyeon Kim, Yu Eun Cheong, Sora Yu, Yong-Su Jin, Kyoung Heon Kim
Summary: The study constructed a yeast strain capable of metabolizing l-fucose in the gut, enhancing its metabolic activity. Through gene expression and metabolic modeling analysis, it was found that the yeast consumes l-fucose via a specific pathway. This study is significant for enhancing the metabolic activity of yeast and other probiotics in the gut.
MICROBIAL CELL FACTORIES
(2022)
Review
Microbiology
Rameesha Abid, Hassan Waseem, Jafar Ali, Shakira Ghazanfar, Ghulam Muhammad Ali, Abdelbaset Mohamed Elasbali, Salem Hussain Alharethi
Summary: Saccharomyces cerevisiae var. boulardii, known for its treatment efficacy against gastrointestinal diseases, can protect the normal gut microbiota and inhibit pathogenic infections. It has antibacterial, antiviral, anti-carcinogenic, antioxidant, anti-inflammatory, and immune-modulatory properties, making it a potential biotherapeutic agent.
Article
Agriculture, Dairy & Animal Science
Haiqing Sun, Fernando Bravo de Laguna, Shuai Wang, Fengju Liu, Liang Shi, Haidi Jiang, Xiaoxia Hu, Peng Qin, Jiajian Tan
Summary: This study found that supplementing lactating sows with Saccharomyces cerevisiae boulardii CNCM I-1079 can shorten farrowing duration, increase feed intake, and minimize backfat losses during lactation. In addition, adding this supplement to piglet diet improves post-weaning performance, possibly due to improved immune status.
JOURNAL OF ANIMAL SCIENCE AND TECHNOLOGY
(2022)
Review
Microbiology
Nicanor Austriaco
Summary: Currently, orally delivered vaccines have advantages over injected vaccines, but approved oral vaccines are limited to diseases that affect the gastrointestinal tract or crucial life cycle stages in the gut. All approved oral vaccines involve live-attenuated or inactivated pathogens. This mini-review explores the potential and challenges of yeast oral vaccine delivery systems for animal and human infectious diseases.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Jolanta Mierzejewska, Patrycja Kowalska, Klaudia Marlicka, Sara Dworakowska, Ewa Sitkiewicz, Maciej Trzaskowski, Agata Gluchowska, Grazyna Mosieniak, Malgorzata Milner-Krawczyk
Summary: This study investigated the ability of extracellular vesicles (EVs) produced by the probiotic yeast Saccharomyces boulardii to transfer and internalize bioactive molecules in human intestinal cells. The results showed that these EVs successfully delivered the anticancer drug doxorubicin to the cells without significant negative effects. Proteomic analysis also identified 541 proteins, providing important information for exploring yeast EVs as a drug delivery system.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Babak Pakbin, Shaghayegh Pishkhan Dibazar, Samaneh Allahyari, Maryam Javadi, Zahra Amani, Alireza Farasat, Sina Darzi
Summary: In this study, the effects of Saccharomyces boulardii supernatant (SBS) on breast cancer cells were investigated. It was found that SBS can induce apoptosis and suppress survivin gene expression, suggesting its potential as an anticancer drug for breast carcinoma.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2022)
Article
Water Resources
Mohaddeseh Zahmatkesh Anbarani, Afsaneh Esmaeili Nasrabadi, Ziaeddin Bonyadi
Summary: The aim of this study was to evaluate the efficiency of S. cerevisiae in removing PS from aqueous solutions. BBD was used to determine the optimal removal conditions. The maximum PS removal efficiency was 98.81% under optimized conditions. Based on the results, it can be concluded that S. cerevisiae can be used as a natural and environmentally friendly biocoagulant to remove PS.
APPLIED WATER SCIENCE
(2023)
Article
Agriculture, Dairy & Animal Science
Valentina Bongiorno, Achille Schiavone, Manuela Renna, Stefano Sartore, Dominga Soglia, Paola Sacchi, Marta Gariglio, Annelisse Castillo, Cecilia Mugnai, Claudio Forte, Chiara Bianchi, Silvia Mioletti, Laura Gasco, Ilaria Biasato, Alberto Brugiapaglia, Federico Sirri, Marco Zampiga, Francesco Gai, Margherita Marzoni, Silvia Cerolini, Sihem Dabbou
Summary: This research investigated the slaughter performance and meat quality of two native Italian chicken breeds, Bionda Piemontese and Bianca di Saluzzo. The results showed that slaughtering at 7 months of age was associated with the best slaughter performance and meat quality characteristics in both breeds.
Article
Plant Sciences
Giulia Mastellone, Arianna Marengo, Barbara Sgorbini, Federica Scaglia, Francesca Capetti, Francesco Gai, Pier Giorgio Peiretti, Patrizia Rubiolo, Cecilia Cagliero
Summary: There is a renewed interest in cannabis-related products due to the rich phytocomplex, fiber, and agricultural features of the plant. The current study chemically characterized fiber-type Cannabis sativa L. samples from Italy and found that the phytocomplex mainly consists of flavonoids and non-psychotomimetic cannabinoids. The freeze-drying method was shown to better preserve the chemical composition of the samples, while the location of the land plot and the growth stage of the plants had minor influences on the chemical pattern. In vitro assays also demonstrated the potential biological activity of these samples and the contribution of non-psychotomimetic cannabinoids.
Article
Agriculture, Dairy & Animal Science
I Biasato, G. Chemello, S. Bellezza Oddon, I Ferrocino, M. R. Corvaglia, C. Caimi, A. Resconi, A. Paul, M. van Spankeren, M. T. Capucchio, E. Colombino, L. Cocolin, F. Gai, A. Schiavone, L. Gasco
Summary: This study investigated the effects of including Hermetia illucens meal in low-fishmeal diets for rainbow trout. The results showed that Hermetia illucens meal can be used in the diets without negatively affecting the growth performance, somatic indices, and histomorphological features of the rainbow trout. Additionally, the utilization of Hermetia illucens meal led to a shift in the gut microbiota, promoting the growth of short-chain fatty acid-producing bacteria and reducing foodborne pathogens.
ANIMAL FEED SCIENCE AND TECHNOLOGY
(2022)
Article
Microbiology
Fabio Rangel, Paula Enes, Laura Gasco, Francesco Gai, Bela Hausmann, David Berry, Aires Oliva-Teles, Claudia R. Serra, Fatima C. Pereira
Summary: The aquaculture industry is rapidly growing, but reliance on wild fish-based meals for carnivorous fish farming is unsustainable. Insect-based diets show promise as fishmeal substitutes, but their impact on farmed fish gut microbiota remains inconclusive and research on microbial functions is scarce.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biology
Fabio Rangel, Rafaela A. Santos, Marta Monteiro, Ana Sofia Lavrador, Laura Gasco, Francesco Gai, Aires Oliva-Teles, Paula Enes, Claudia R. Serra
Summary: As the demand for fish increases due to the growing human population, aquaculture has become one of the fastest growing industries. However, the use of unsustainable fish meal as the main protein source in aquafeeds needs to be replaced. Recently, seven insect species have been approved as potential ingredients for animal feeds in Europe. Unfortunately, chitin, a component found in insects, is indigestible for economically valuable fish species, leading to lower fish performance. This study aimed to isolate probiotic bacteria from the gastrointestinal tract of European sea bass that can produce chitinases to improve the use of diets containing high levels of insect meal.
Article
Fisheries
Thomas Bousdras, Konstantinos Feidantsis, Nikolas Panteli, Stavros Chatzifotis, Giovanni Piccolo, Laura Gasco, Francesco Gai, Efthimia Antonopoulou
Summary: This study investigated the effects of dietary inclusion of Tenebrio molitor larvae on the cytoprotection, cell death pathways, antioxidant defense, and intermediate metabolism in the heart, muscle, and digestive tract of gilthead seabream and European sea bass. The results showed that the inclusion of Tenebrio molitor larvae had different effects on the cellular responses of the two fish species.
AQUACULTURE NUTRITION
(2022)
Article
Food Science & Technology
Alfonsina Fiore, Ida Treglia, Gianni Ciccaglioni, Marco Francesco Ortoffi, Antonietta Gattuso
Summary: Changing eating habits and rising demand of food have led to an increase in foodborne diseases, particularly in industrialized countries. Contaminated ready-to-eat food can transmit Listeria monocytogenes, a foodborne pathogen responsible for listeriosis. This study successfully applied a colorimetric Loop-mediated isothermal amplification (LAMP) assay to rapidly detect L. monocytogenes in experimentally contaminated RTE meat samples. The results showed high specificity and sensitivity compared to traditional methods, suggesting that colorimetric LAMP assays can be used as a screening tool for L. monocytogenes in ready-to-eat meat food.
Article
Fisheries
Francesco Gai, Giovanni Marco Cusimano, Giulia Maricchiolo, Letteria Caccamo, Christian Caimi, Elisabetta Macchi, Martina Meola, Anna Perdichizzi, Gennaro Tartarisco, Laura Gasco
Summary: The study investigated the effects of replacing fish meal with partially defatted Hermetia illucens larvae meal (HIM) in the diet of adult gilthead sea bream. Results showed that HIM can be used as an alternative protein source up to a 18.4% inclusion level without impacting growth performance, gill cortisol, morphometric and biometric indexes, ADCs, and gut enzymes.
AQUACULTURE RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Francesco Gai, Michal A. Janiak, Katarzyna Sulewska, Pier Giorgio Peiretti, Magdalena Karamac
Summary: The aim of this research was to identify the growth stage of flax with the highest antioxidant capacity. It was found that the aerial parts of flax, from stem extension to mature seeds, contained the highest content of phenolic compounds, particularly mono- and di-C-glycosyl flavones. These extracts showed high antiradical and antioxidant activity, making them suitable for use as nutraceuticals and components of functional foods.
Article
Agriculture, Dairy & Animal Science
Elena Colombino, Mark Karimi, Mai Anh Ton Nu, Andrea Aurora Tilatti, Sara Bellezza Oddon, Franco Calini, Cinzia Bergamino, Edoardo Fiorilla, Marta Gariglio, Francesco Gai, Maria Teresa Capucchio, Achille Schiavone, Laura Gasco, Ilaria Biasato
Summary: The study examines the effects of a thermomechanical, enzyme-facilitated, coprocessed yeast and soybean meal on the growth performance, organ weights, leg health, and gut development in broiler chickens. The results suggest that feeding high levels of this additive during the prestarter and starter phases can improve the growth performance and gut immunity of broilers.
Article
Agriculture, Dairy & Animal Science
Anna Perdichizzi, Martina Meola, Letteria Caccamo, Gabriella Caruso, Francesco Gai, Giulia Maricchiolo
Summary: In animal science, dietary supplementation with probiotics, specifically Saccharomyces cerevisiae var. boulardii (LSB), has been shown to enhance stress tolerance, disease prevention, and growth in aquatic animals. This study aimed to evaluate the effects of LSB supplementation on the welfare and health of juvenile European sea bass. The results suggest that LSB has an immunomodulatory action, potentially reducing gut inflammation and enhancing disease prevention in sea bass.
Article
Agriculture, Dairy & Animal Science
Andres L. Martinez Marin, Marta Gariglio, Ilaria Biasato, Laura Gasco, Achille Schiavone
Summary: This study conducted a meta-analysis to determine the effects of increasing dietary levels of black soldier fly larvae meal (BSFLM) on broiler performance and developed prediction models for the apparent metabolisable energy corrected to zero nitrogen balance (AMEn) of BSFLM. The results showed that including 15% BSFLM in the diet did not have negative effects on broiler growth performance. Regression analysis and artificial neural network (ANN) models were used to accurately predict the AMEn of BSFLM.
ITALIAN JOURNAL OF ANIMAL SCIENCE
(2023)
Article
Agriculture, Dairy & Animal Science
Vlastimil Stejskal, Hung Quang Tran, Marketa Prokesov, Mahyar Zare, Tatyana Gebauer, Tomas Policar, Christian Caimi, Francesco Gai, Laura Gasco
Summary: This study investigated the effects of adding defatted black soldier fly meal (HIM) at different inclusion levels on the growth performance, nutrient digestibility, fillet quality, and economic and environmental sustainability of pikeperch. The results showed that adding HIM at an inclusion level of 18% did not adversely affect the growth performance parameters of pikeperch and had environmental benefits associated with land use and marine resources required for fish farming.