Article
Electrochemistry
Veronika Svitkova, Vlastimil Vyskocil
Summary: Cyclic voltammetry and differential pulse voltammetry were used to study the behavior of methylene blue at a mercury meniscus-modified silver solid amalgam electrode. Electrochemical impedance spectroscopy was used for verification and comparison. It was found that the reduction signal of methylene blue was significantly higher at the DNA-modified silver amalgam electrode compared to the unmodified electrode, which correlated with changes in charge transfer resistance. Optimized parameters were determined for further biosensor development and applications.
JOURNAL OF SOLID STATE ELECTROCHEMISTRY
(2022)
Article
Pharmacology & Pharmacy
Cassiele T. Cesco, Artur J. M. Valente, Alexandre T. Paulino
Summary: Chitosan/DNA blend hydrogel and chitosan/pectin blend hydrogel were synthesized for releasing methylene blue, and characterized by various techniques. The study revealed that the methylene blue loading had different impacts on the hydrogels, with higher release rates observed in simulated gastric fluid. The release kinetics of methylene blue in the hydrogels followed a pseudo-second-order mechanism.
Article
Chemistry, Physical
S. Sivakumar, T. Daniel Thangadurai, N. Manjubaashini, D. Nataraj
Summary: This research focuses on the photocatalytic degradation of textile dyes using graphitic carbon nitrides (g-C3N4) under sunlight. However, the charge carrier recombination of g-C3N4 limits its photocatalytic application. To overcome this limitation, thin-layered molybdenum disulfide (MoS2) was deposited on g-C3N4 using an impregnation approach. The resulting MoS2/g-C3N4 heterojunction nanocomposite showed improved photocatalytic performance due to the homogeneous distribution of MoS2 and efficient charge separation.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2022)
Article
Chemistry, Analytical
Xiaoqian Wei, Shanlin Wang, Yujuan Zhan, Tianhan Kai, Ping Ding
Summary: We report a methylene blue-modified electrochemical aptamer sensor for determining microcystin-LR. The sensor signal transduction is based on changes in conformation and position of methylene blue induced by binding with microcystin-LR. We optimized the parameters and evaluated the sensor's electrochemical performance using square wave voltammetry. The reliability of the method was verified by testing environmental samples, and the sensor was shown to be reusable and regenerable with good accuracy and reproducibility. This rapid, reagent-free, and sensitive sensing platform will facilitate routine monitoring of microcystin-LR.
Article
Chemistry, Analytical
Jianmei Wang, Hong Zhou, Jing Liu, Jin He, Jingquan Liu, Wenrong Yang
Summary: A unique label-free electrochemical DNA biosensor based on gold nanoparticles (GNPs) mediated electron transfer has been developed for sensitive and accurate detection of target DNA. Experimental results showed that the different states of DNA monolayers and the sizes of GNPs significantly affect the electron transfer, with a detection limit of 1.47 pM.
MICROCHEMICAL JOURNAL
(2021)
Article
Chemistry, Analytical
Maria A. Komkova, Alexei K. Orlov, Andrei A. Galushin, Egor A. Andreev, Arkady A. Karyakin
Summary: The catalytic current of PQQ-GDH immobilized over electropolymerized MG increased five times with the addition of a freely diffusing mediator, which is lower than other glucose electrodes; The thermodynamics of the polyMG| PQQ-GDH electrode is determined by enzyme-catalyzed reaction, showing high catalytic activity in the presence of glucose; Successful glucose detection in human sweat using the enzyme electrode confirms the attractiveness of the bioelectrocatalytic system for advanced biosensors and biofuel cells.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Physical
Gabriela Stinga, Adriana Baran, Alina Iovescu, Florina Branzoi, Dan-Florin Anghel
Summary: In this study, attractive smart and emissive nanomaterials were designed by embedding methylene blue in complexes using a supramolecular host-guest strategy. The photophysical properties of the assemblies were highlighted, and the formation of different complexes at varying surfactant concentrations was analyzed. The results suggest potential applications in chemical sensing and imaging.
JOURNAL OF MOLECULAR LIQUIDS
(2021)
Article
Chemistry, Analytical
M. S. Kumar, Ruchira Nandeshwar, Shailesh B. Lad, Kirti Megha, Maheshwar Mangat, Adrian Butterworth, Charles W. Knapp, Mara Knapp, Paul A. Hoskisson, Damion K. Corrigan, Andrew C. Ward, Kiran Kondabagil, Siddharth Tallur
Summary: A low-cost electrochemical DNA biosensor based on PCB electrodes is presented for monitoring virus amplicons in wastewater, offering advantages such as high sensitivity, reusability, and ease of cleaning. It is capable of detecting SARSCoV-2 and other pathogens, providing valuable insights for researchers in the field.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Biochemistry & Molecular Biology
Natalia A. A. Samoylova, Artem P. P. Gureev, Vasily N. N. Popov
Summary: Cisplatin, a platinum-based cytostatic drug used for cancer treatment, can cause nephrotoxicity by damaging renal mitochondria and mtDNA. This study found that exposure to cisplatin increased lipid peroxidation and induced damage to renal mtDNA. On the other hand, methylene blue protected kidney mitochondria by activating the Nrf2/ARE signaling pathway and promoting the expression of antioxidant genes and genes related to mtDNA repair. This research highlights the significance of using methylene blue to protect kidney mitochondria from cisplatin-induced damage in medicine.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemical Research Methods
Veronika Svitkova, Katarina Nemcekova, Vlastimil Vyskocil
Summary: In this study, a mercury meniscus-modified silver solid amalgam electrode was used for the detection of UV-induced DNA damage. The integrity of the double-stranded DNA layer was indirectly detected using methylene blue reduction, and different wavelengths of UV irradiation resulted in different electrochemical signals.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Inorganic & Nuclear
Junhong Liu, Jinhai Yuan, Xuanlan Luo, Haikun Hu, Ziyi Yang, Qi Sun, Xiaopeng Hou, Di Xu
Summary: A novel organic/inorganic heterostructured photocatalyst binary nanocomposite was prepared by the solvothermal method, showing excellent photocatalytic performance and stability in the degradation of organic dyes.
JOURNAL OF SOLID STATE CHEMISTRY
(2023)
Article
Chemistry, Physical
Jorge C. Herrera-Luna, Till J. B. Zaehringer, Jorge C. Herrera-Luna, M. Consuelo Jimenez, Christoph Kerzig, Raul Perez-Ruiz
Summary: The design and development of new triplet-triplet annihilation upconversion (TTA-UC) systems combining triplet sensitizers with acceptor compounds have attracted considerable interest. Sensitizers made from purely organic dyes rather than transition-metal complexes appear to be more convenient from an environmental point of view. The versatile properties of a new asymmetric BODIPY were demonstrated, showing its suitability as a sensitizer in a bimolecular TTA-UC system. The TTA-UC system showed highly efficient photocatalytic behavior, leading to successful conversion of green to blue light and subsequent activation of aryl chlorides for various chemical reactions.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2023)
Article
Chemistry, Multidisciplinary
Kaiyu Fu, Ji-Won Seo, Vladimir Kesler, Nicolo Maganzini, Brandon D. Wilson, Michael Eisenstein, Boris Murmann, H. Tom Soh
Summary: This study successfully optimized electrochemical aptamer biosensors on nanoporous electrodes to improve sensitivity and proposed a novel mechanism. By tuning pore size, probe density, and electrochemical measurement parameters, it was experimentally verified that electron transfer is accelerated within nanostructured electrodes, leading to enhanced sensitivity.
Article
Electrochemistry
Franziska Schachinger, Stefan Scheiblbrandner, Kwankao Karnpakdee, Erik Breslmayr, Su Ma, Roland Ludwig
Summary: Cytochromes are a group of proteins used in bioelectrochemistry as electron shuttles between biocatalysts and electrodes. Researchers identified 58 cytochromes out of 850 screened hemoproteins that met the requirements for electron transfer. These cytochromes have suitable structural and electrochemical properties, including a small size, exposed heme cofactor, reversible redox process, and fast electron transfer. The study confirms their electrochemical reversibility and demonstrates that c-type cytochromes have faster electron uptake compared to b-type cytochromes. The findings support the design of bioelectrocatalysts with high electron transfer rates.
ELECTROCHIMICA ACTA
(2023)
Article
Electrochemistry
Fernando Otero, Kim Shortall, Urszula Salaj-Kosla, Syed A. M. Tofail, Edmond Magner
Summary: An electrochemical biosensor was developed using a hairpin DNA probe labeled with methylene blue for detecting the TP53 gene. The biosensor could selectively detect a single nucleotide polymorphism in the TP53 gene affecting residue 175 of the p53 protein, with a more pronounced response at shorter hybridization times.
ELECTROCHIMICA ACTA
(2021)
Review
Chemistry, Analytical
Kamila Malecka, Edyta Mikula, Elena E. Ferapontova
Summary: The improvement in outcomes for various types of cancer in recent years is attributed to early tumor detection and widespread use of screening tests, with non-invasive, fast, and accurate diagnostic devices playing a crucial role. Biosensors utilizing aptamers in cancer diagnosis offer advantages such as in-vitro aptamer production, cost-effective chemical synthesis, and thermal stability, while electrochemical biosensing approaches provide sensitive, accurate, and affordable sensing methods. This review evaluates the design strategies and analytical performance of electrochemical aptamer-based biosensors for cancer diagnosis, highlighting their future prospects in cancer diagnostics.
Article
Nanoscience & Nanotechnology
Kamila Malecka, Elena E. Ferapontova
Summary: Thrombin is a central biomarker in cardiovascular diseases and Alzheimer's disease development, but its electroanalysis is complicated by low physiological concentrations and nonspecific binding. A new electrochemical method was developed to detect femtomolar levels of thrombin in serum and CSF using G4-hemin DNAzyme, which is simple, sensitive, and selective, providing a new tool for ultrasensitive electrocatalytic assays.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Engineering, Environmental
Natan Blommaerts, Nick Hoeven, Daniel Arenas Esteban, Rui Campos, Myrjam Mertens, Rituraj Borah, Antonella Glisenti, Karolien De Wael, Sara Bals, Silvia Lenaerts, Sammy W. Verbruggen, Pegie Cool
Summary: In this study, a Ti-Beta zeolite was utilized in gas phase photocatalytic CO2 reduction with Pt and Pd nanoparticles to enhance efficiency and selectivity, achieving higher turnover frequency. Selectivity towards CO or CH4 could be adjusted by adding different nanoparticles, with significantly improved performance compared to the commercial benchmark P25.
CHEMICAL ENGINEERING JOURNAL
(2021)
Article
Electrochemistry
Naghmehalsadat Mirbagheri, Rui Campos, Elena E. Ferapontova
Summary: This study investigated the effect of different capping ligands and electrode substrates on the electrocatalysis of iridium oxide nanoparticles for water oxidation reaction. The results showed that the hydrophilic properties of the electrode substrate could affect the morphology and catalytic performance of the formed iridium oxide films.
Article
Chemistry, Physical
Myrthe Van Hal, Rui Campos, Silvia Lenaerts, Karolien De Wael, Sammy W. Verbruggen
Summary: Research has shown the potential of photofuel cells for waste water treatment and energy recovery. This study introduces an autonomously operating air purification device for sustainable electricity generation. Experiment results demonstrate that tungsten oxide-based photocatalysts outperform titanium dioxide-based ones in gas-phase PFC operation, achieving high fuel-to-electricity conversion rates.
APPLIED CATALYSIS B-ENVIRONMENTAL
(2021)
Review
Chemistry, Analytical
Elise Daems, Giulia Moro, Rui Campos, Karolien De Wael
Summary: Aptamers are versatile biorecognition elements with wide applicability, requiring thorough characterization of their binding performance in the presence of the target for successful use. Various multi-analytical approaches are available to understand the aptamer-target interaction, highlighting the importance of considering the final application and limitations of analytical tools in the characterisation process.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Giulia Moro, Cedrix J. Dongmo Foumthuim, Marco Spinaci, Ettore Martini, Dafne Cimino, Eleonora Balliana, Peter Lieberzeit, Flavio Romano, Achille Giacometti, Rui Campos, Karolien De Wael, Ligia Maria Moretto
Summary: There is an urgent need for sensing strategies to screen perfluoroalkyl substances (PFAS) in aqueous matrices. Changes in fluorinated self-assembled monolayers (SAM) with different architectures were studied upon exposure to both long and short-chain PFAS. The applicability of fluorinated SAM in PFAS sensing was evaluated using electrochemical impedance spectroscopy and voltam-metric techniques, supported by molecular dynamics simulations. Pinhole/defect-free fluorinated SAM were found to be applicable to long-chain PFAS screening using a switch-on sensing strategy, while a switch-off sensing strategy was reported for screening of both short/long-chain PFAS.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Rocio Canovas, Elise Daems, Rui Campos, Sofie Schellinck, Annemieke Madder, Jose C. Martins, Frank Sobott, Karolien De Wael
Summary: This work presents a proof-of-concept assay for the detection and quantification of small molecules based on aptamer recognition and electrochemiluminescence (ECL) readout. The research demonstrates the successful use of the testosterone-binding aptamer as a novel method for detecting and quantifying testosterone. The combination of aptamer recognition and ECL readout allows for selective detection and quantification of testosterone with high sensitivity.
Review
Chemistry, Physical
Elena E. Ferapontova
Summary: This article discusses the application of electrochemical methods in the study of neurodegenerative disorders, with a focus on recent advancements in bioelectroanalysis using aptamer- and antibody-modified electrodes.
CURRENT OPINION IN ELECTROCHEMISTRY
(2022)
Article
Chemistry, Analytical
Ana Diaz-Fernandez, Elena E. Ferapontova
Summary: This study introduces a simple and fast liquid biopsy method for analyzing the overexpressed HER-2/neu protein in aggressive cancers, which shows high sensitivity and low cost.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Lucia Gomez-Arconada, Ana Diaz-Fernandez, Elena E. Ferapontova
Summary: Serine protease thrombin is a strong neurotoxin and Alzheimer's disease biomarker. In this study, the O-2-dependent aptamer assay for thrombin was adapted to gold screen-printed electrodes (Au SPE) for point-of-care testing (POCT). The assay showed enhanced electrocatalytic activity in O2 reduction upon thrombin binding to the aptamer, and 0.5 fM thrombin was detected with a logarithmic linear range extending to 100 fM. The disposable aptasensor met basic POCT requirements but special surface pre-treatment and modification protocols were needed for practical applications.
Article
Chemistry, Multidisciplinary
Maciej Sosna, Elena E. Ferapontova
Summary: The oriented coupling of redox enzymes to electrodes by reconstitution onto redox cofactors poses the requirements for a suitable self-assembled monolayer (SAM) design. The kinetics of electron transfer in binary SAMs composed of alkanethiols and hemin-conjugated linker molecules depends on the SAM composition and hemin surface density. The use of SAM-modified electrodes with loosely dispersed redox centers and rigid molecular wires is important for the effective on-surface reconstitution of proteins and enzymes.
Article
Chemistry, Analytical
Ana Diaz-Fernandez, Alexey Ferapontov, Mikkel Holm Vendelbo, Elena E. Ferapontova
Summary: Researchers have developed a cellulase-linked sandwich bioassay on magnetic beads for detecting the levels of protein HER-2/neu in blood. This method is simple, low-cost, and enables fast and accurate liquid biopsy analysis.
ACS MEASUREMENT SCIENCE AU
(2023)
Article
Biochemistry & Molecular Biology
Giulia Moro, Rui Campos, Elise Daems, Ligia Maria Moretto, Karolien De Wael
Summary: The haem group is a promising redox probe for albumin-based voltammetric sensors, with reversible redox behaviour and binding kinetics that depend on hSA's conformation and the presence of other ligands. Hemin was tested as a redox probe using PFOA as a model analyte, and its binding kinetics were investigated using UV-Vis spectroscopy and electrochemical methods. The study provides insight into the application of hemin as a redox probe in albumin-based voltammetric sensing strategies.
BIOELECTROCHEMISTRY
(2023)