Review
Cell Biology
Jan K. Hennigs, Christiane Matuszcak, Martin Trepel, Jakob Koerbelin
Summary: Endothelial cells play crucial roles in facilitating physiological processes throughout the body, demonstrating tremendous cellular diversity. Different endothelial cell populations adapt to the specific needs of various organs and tissues, influencing vascular tone, blood coagulation, and cellular adhesion.
Article
Chemistry, Multidisciplinary
Md Kabir, Ning Sun, Xiaoping Hu, Tiphaine C. Martin, Jingjie Yi, Yue Zhong, Yan Xiong, H. Umit Kaniskan, Wei Gu, Ramon Parsons, Jian Jin
Summary: With the novel Acetylation Targeting Chimera (AceTAC) strategy, the p53 tumor suppressor protein can be acetylated successfully. The first p53Y220C AceTAC, MS78, recruits histone acetyltransferase p300/CBP to acetylate the p53Y220C mutant. MS78 effectively suppresses the proliferation and clonogenicity of cancer cells with the p53Y220C mutation and induces upregulation of TRAIL apoptotic genes and downregulation of DNA damage response pathways. In conclusion, the AceTAC strategy provides a generalizable platform for targeting proteins, such as tumor suppressors, via acetylation.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Chemistry, Multidisciplinary
Ming Wu, Hao Li, Cao Zhang, Yingchao Wang, Cuilin Zhang, Yuting Zhang, Aoxue Zhong, Da Zhang, Xiaolong Liu
Summary: This study develops a genetically engineered adenovirus for delivering CRISPR/Cas9 to edit the PD-L1 gene with high efficiency in liver cancer cells. The adenovirus is loaded into a silk-gel carrier, which enables effective tumor growth inhibition and T cell-mediated antitumor immune response.
Article
Chemistry, Multidisciplinary
Silvia Arifi, Julian A. Marschner, Julius Pollinger, Laura Isigkeit, Pascal Heitel, Astrid Kaiser, Lennart Obeser, Georg Hoefner, Ewgenij Proschak, Stefan Knapp, Apirat Chaikuad, Jan Heering, Daniel Merk
Summary: The lipid-sensing transcription factor PPAR γ can bind to antidiabetic TZD drugs, oxidized vitamin E metabolites, and vitamin E mimetic garcinoic acid. While the effects of the second binding on PPAR γ activity are unclear, a selective ligand of the second site has been developed, revealing potential noncanonical regulation of PPAR γ activities. This alternative binding diminishes FOXO signaling and may have therapeutic applications.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Chemistry, Multidisciplinary
Ya-Qiong Yan, Jia-Qi Wang, Lingze Zhang, Pei-Pei Yang, Xin-Wei Ye, Cong Liu, Da-Yong Hou, Wen-Jia Lai, Jie Wang, Xiang-Zhong Zeng, Wanhai Xu, Lei Wang
Summary: This article introduces an in vivo screening method of the OBOC library based on localized instillation beads, which is used to discover targeting peptides. By constructing a cavity inside a transplanted tumor of a mouse and injecting the OBOC heptapeptide library, three MDAMB-231 tumor-targeting peptides were discovered after an efficient elution process. The best peptide was verified to have 1.9-fold higher tumor accumulation than the commonly used targeting peptide RGD in vivo. Finally, two potential targeting proteins related to our targeting peptide were discovered. The in vivo LIB screening method expands the scope of OBOC peptide screening applications to discover targeting peptides feasibly and reliably.
Review
Food Science & Technology
Mamali Das, Kasi Pandima Devi, Tarun Belwal, Hari Prasad Devkota, Devesh Tewari, Adeleh Sahebnasagh, Seyed Fazel Nabavi, Hamid Reza Khayat Kashani, Mahsa Rasekhian, Suowen Xu, Mehran Amirizadeh, Kiumarth Amini, Maciej Banach, Jianbo Xiao, Safieh Aghaabdollahian, Seyed Mohammad Nabavi
Summary: Vascular diseases are caused by abnormal endothelial response, resulting in atherosclerosis, hypercholesterolemia, etc. Polyphenols have antioxidative, anti-inflammatory, and anti-hypertensive properties, beneficial for preventing endothelial dysfunction.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Virology
Alejandro J. Nicola Candia, Matias Garcia Fallit, Jorge A. Pena Agudelo, Melanie Perez Kuper, Nazareno Gonzalez, Mariela A. Moreno Ayala, Emilio De Simone, Carla Giampaoli, Noelia Casares, Adriana Seilicovich, Juan Jose Lasarte, Flavia A. Zanetti, Marianela Candolfi
Summary: This study found that Foxp3 has protumoral intrinsic effects in breast cancer cells, and gene therapy-mediated blockade of Foxp3 could be a therapeutic strategy to improve the response of these tumors to standard treatment.
Article
Nanoscience & Nanotechnology
Xiao-yu Dai, Li-jun Ren, Lang Yan, Ji-qian-zhu Zhang, Yi-fan Dong, Tao-lin Qing, Wen-jing Shi, Jin-feng Li, Fang-yuan Gao, Xiao-fang Zhang, Yi-jun Tian, Yu-ping Zhu, Jiang-bo Zhu, Ji-kuai Chen
Summary: This study demonstrated that MWCNTs can affect endothelial cell activity, disrupt tube formation and cell migration ability, subsequently affecting angiogenesis. Furthermore, the VEGF-Akt-eNOS axis plays a crucial role in MWCNT-induced endothelial cell injury.
Article
Multidisciplinary Sciences
Zengpeng Han, Nengsong Luo, Wenyu Ma, Xiaodong Liu, Yuxiang Cai, Jiaxin Kou, Jie Wang, Lei Li, Siqi Peng, Zihong Xu, Wen Zhang, Yuxiang Qiu, Yang Wu, Chaohui Ye, Kunzhang Lin, Fuqiang Xu
Summary: Viral tracers are important for studying neural circuits and brain diseases. AAV11 is a powerful retrograde viral tracer that allows for analyzing circuit connectivity and shows superior astrocytic tropism compared to commonly used AAV vectors. AAV11 can be used for monitoring neuronal activities and studying neuron-astrocyte connections.
NATURE COMMUNICATIONS
(2023)
Review
Pharmacology & Pharmacy
Yuichi Hattori, Kohshi Hattori, Takuji Machida, Naoyuki Matsuda
Summary: Vascular endotheliitis plays a crucial role in immune responses, inflammation, and infectious pathologies. It is strongly associated with the pathogenesis of diseases such as COVID-19 and sepsis. Protecting endothelial cells and reversing vascular endotheliitis can be the main therapeutic goal for these diseases.
BIOCHEMICAL PHARMACOLOGY
(2022)
Article
Oncology
Ji Yoon Lee, Soojin Park, A-Reum Han, Hee-Sun Hwang, Hee-Je Kim
Summary: In this study, we developed 12 peptides targeting FLT4 for clinical application and found that peptide 4 (P4) restored the functionality of dysfunctional NK cells and T cells in patients with AML. P4 treatment also increased the expression of IFN-gamma and decreased the number of regulatory T cells. Overall, we demonstrated the therapeutic potential of modulating lymphocytes using FLT4-targeting peptides for advanced approaches against AML.
CANCER IMMUNOLOGY IMMUNOTHERAPY
(2023)
Article
Biochemistry & Molecular Biology
Indira Singaram, Ashutosh Sharma, Shashank Pant, Muyun Lihan, Mi-Jeong Park, Melissa Pergande, Pawanthi Buwaneka, Yusi Hu, Nadim Mahmud, You-Me Kim, Stephanie Cologna, Vladimir Gevorgyan, Irum Khan, Emad Tajkhorshid, Wonhwa Cho
Summary: Membrane lipids control the cellular activity of kinases containing the SH2 domain. In this study, new nonlipidic small molecule inhibitors of the lipid-SH2 domain interaction were developed to block the cellular activity of their host proteins. The research shows that targeting lipid-protein interaction is a powerful approach to developing new small molecule drugs.
NATURE CHEMICAL BIOLOGY
(2023)
Article
Pharmacology & Pharmacy
Mengting Chen, Xiaocui Fang, Rong Du, Jie Meng, Jingyi Liu, Mingpeng Liu, Yanlian Yang, Chen Wang
Summary: A new therapeutic strategy for chronic myeloid leukemia (CML) was discovered utilizing a peptide (WIP2W) that specifically inhibits the WT1 protein, inducing cell cycle arrest and cytotoxicity. A micellar formulation (M-WIP2W) enhances intra-tumoral accumulation and effectively inhibits tumor growth.
Review
Immunology
Abbey Lightfoot, Helen M. McGettrick, Asif J. Iqbal
Summary: Leukocyte recruitment to the injury site is crucial for regulating inflammatory responses, and research on the immunoregulatory roles of galectin family members suggests that they could be a promising target for therapeutic intervention in inflammatory diseases. This review focuses on summarizing the current understanding of galectins in leukocyte trafficking during inflammation and the clinical approaches being developed to target these interactions for treating inflammatory diseases.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Medicine, Research & Experimental
Yuyi Wang, Ombretta Salvucci, Hidetaka Ohnuki, Andy D. Tran, Taekyu Ha, Jing-Xin Feng, Michael DiPrima, Hyeongil Kwak, Dunrui Wang, Yanlin Yu, Michael Kruhlak, Giovanna Tosato
Summary: SHP2 is essential for promoting endothelial cell survival and growth in tumor vasculature remodeling. Inhibiting SHP2 activity leads to reduced tumor growth and degeneration of tumor vasculature. Combination of SHP2 inhibitors with Ang2/TIE2 inhibitors shows promise in effectively targeting the tumor endothelium.
EMBO MOLECULAR MEDICINE
(2021)
Article
Oncology
Barbara Muz, Nilantha Bandara, Cedric Mpoy, Jennifer Sun, Kinan Alhallak, Feda Azab, Buck E. Rogers, Abdel Kareem Azab
CANCER BIOLOGY & THERAPY
(2020)
Article
Chemistry, Multidisciplinary
Micah John Luderer, Barbara Muz, Kinan Alhallak, Jennifer Sun, Katherine Wasden, Nicole Guenthner, Pilar de la Puente, Cinzia Federico, Abdel Kareem Azab
PHARMACEUTICAL RESEARCH
(2019)
Letter
Oncology
Barbara Muz, Feda Azab, Mark Fiala, Justin King, Daniel Kohnen, William E. Fogler, Ted Smith, John L. Magnani, Ravi Vij, Abdel Kareem Azab
BLOOD CANCER JOURNAL
(2019)
Correction
Chemistry, Organic
Daniela Imperio, Barbara Muz, Abdel Kareem Azab, Silvia Fallarini, Grazia Lombardi, Luigi Panza
EUROPEAN JOURNAL OF ORGANIC CHEMISTRY
(2020)
Article
Oncology
Jennifer Sun, Barbara Muz, Kinan Alhallak, Matea Markovic, Shannon Gurley, Zhe Wang, Nicole Guenthner, Katherine Wasden, Mark Fiala, Justin King, Daniel Kohnen, Noha Nabil Salama, Ravi Vij, Abdel Kareem Azab
Article
Multidisciplinary Sciences
Cinzia Federico, Kinan Alhallak, Jennifer Sun, Kathleen Duncan, Feda Azab, Gail P. Sudlow, Pilar de la Puente, Barbara Muz, Vaishali Kapoor, Luna Zhang, Fangzheng Yuan, Matea Markovic, Joseph Kotsybar, Katherine Wasden, Nicole Guenthner, Shannon Gurley, Justin King, Daniel Kohnen, Noha N. Salama, Dinesh Thotala, Dennis E. Hallahan, Ravi Vij, John F. DiPersio, Samuel Achilefu, Abdel Kareem Azab
NATURE COMMUNICATIONS
(2020)
Review
Oncology
Barbara Muz, Anas Abdelghafer, Matea Markovic, Jessica Yavner, Anupama Melam, Noha Nabil Salama, Abdel Kareem Azab
Summary: This review focuses on the eradication of cancer by targeting the endothelium surface protein E-selectin with a novel drug, uproleselan, which has shown effectiveness in inhibiting cancer cell dissemination and improving patients' overall survival by disrupting the tumor microenvironment.
Article
Multidisciplinary Sciences
Tobias Hartwig Bunning, Luigi Panza, Abdel Kareem Azab, Barbara Muz, Silvia Fallarini, Daniela Imperio
Summary: Boron Neutron Capture Therapy (BNCT) shows promise in treating non-curable cancers, with a new boron compound being discovered to selectively accumulate in tumor tissue. Hypoxia poses a challenge in cancer treatment, but a new compound exhibits toxicity in multiple myeloma cells and consistent intracellular boron accumulation.
Article
Oncology
Kinan Alhallak, Pilar de la Puente, Amanda Jeske, Jennifer Sun, Barbara Muz, Michael P. Rettig, Ilyas Sahin, Ellen L. Weisberg, James D. Griffin, John L. Reagan, John F. DiPersio, Abdel Kareem Azab
Summary: 3D tissue engineered plasma cultures (3DTEPC) derived from CML, AML and CLL patients supported the growth of primary leukemia cells and induced drug resistance, providing a relevant tool for drug screening and personalized medicine.
LEUKEMIA & LYMPHOMA
(2021)
Article
Multidisciplinary Sciences
Jingzhu Zhang, Adi Cohen, Bo Shen, Liming Du, Alpaslan Tasdogan, Zhiyu Zhao, Elizabeth J. Shane, Sean J. Morrison
Summary: The study revealed that PTH promotes osteolectin expression and its interaction mediates part of the bone formation response to PTH, while SOSTi does not affect osteolectin levels or require it for bone formation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Immunology
Magdalena Massalska, Marzena Ciechomska, Ewa Kuca-Warnawin, Tomasz Burakowski, Anna Kornatka, Anna Radzikowska, Dariusz Pawlak, Barbara Muz, Adrianna Loniewska-Lwowska, Andrzej Palucha, Pawel Maldyk, Wlodzimierz Maslinski
Summary: This study sheds light on immunosuppressive aspects present or induced in the bone marrow, and confirms the potential of IDO-mediated pathways in the periphery, which could be promising candidates for therapeutic strategies in RA.
JOURNAL OF INFLAMMATION RESEARCH
(2022)
Article
Multidisciplinary Sciences
Jingzhu Zhang, Liming Du, Bethany Davis, Zhimin Gu, Junhua Lyu, Zhiyu Zhao, Jian Xu, Sean J. Morrison, Francesca V. Mariani
Summary: Osteolectin is a growth factor that activates the Wnt pathway and promotes bone elongation by binding to Integrin a11. Its deficiency leads to shorter bones in mice, and reduced growth plate chondrocyte proliferation and bone elongation. Furthermore, a single-nucleotide variant associated with reduced height and plasma Osteolectin levels was found downstream of Osteolectin. This study establishes Osteolectin/Integrin a11 as regulators of bone elongation and body length in both mice and humans.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Meeting Abstract
Oncology
Kinan Alhallak, Jennifer Sun, Barbara Muz, Christopher Egbulefu, Pilar de la Puente, Cinzia Federico, Justin King, Danny Kohnen, Ravi Vij, Samuel Achilefu, Abdel Kareem Azab
CANCER IMMUNOLOGY RESEARCH
(2020)
Meeting Abstract
Oncology
Barbara Muz, Pilar de la Puente, Shruti Shah, Cinzia Federico, Feda Azab, Justin King, Mark Fiala, Rui Tang, Partha Karmakar, Ravi Vij, Samuel Achilefu, Abdel Kareem Azab
CANCER IMMUNOLOGY RESEARCH
(2020)
Meeting Abstract
Oncology
Jennifer Sun, Barbara Muz, Kinan Alhallak, Christopher Egbulefu, Justin King, Danny Kohnen, Mark Fiala, Benjamin Cappocia, Pamela Manning, Samuel Achilefu, Ravi Vij, Abdel Kareem Azab
CANCER IMMUNOLOGY RESEARCH
(2020)
Article
Medicine, Research & Experimental
Marijn A. Scheijde-Vermeulen, Lennart A. Kester, Liset Westera, Bastiaan B. J. Tops, Friederike A. G. Meyer-Wentrup
Summary: This study aimed to evaluate the feasibility of integrating state-of-the-art sequencing techniques and flow cytometry into the diagnostic workup of pediatric lymphoma. The results showed that this integration is not only feasible but also provides additional diagnostic information.
LABORATORY INVESTIGATION
(2024)
Article
Medicine, Research & Experimental
Enrico Berrino, Sara Erika Bellomo, Anita Chesta, Paolo Detillo, Alberto Bragoni, Amedeo Gagliardi, Alessio Naccarati, Matteo Cereda, Gianluca Witel, Anna Sapino, Benedetta Bussolati, Gianni Bussolati, Caterina Marchi
Summary: Formalin-fixed paraffin-embedded (FFPE) samples are crucial for tissue-based analysis in precision medicine, but the quality of these samples can affect the reliability of sequencing data. The use of acid-deprived fixatives guarantees the highest DNA preservation and sequencing performance, enabling more complex molecular profiling of tissue samples.
LABORATORY INVESTIGATION
(2024)
Article
Medicine, Research & Experimental
Roope A. Kallionpaa, Sirkku Peltonen, Kim My Le, Eija Martikkala, Mira Jaaskelainen, Elnaz Fazeli, Pilvi Riihila, Pekka Haapaniemi, Anne Rokka, Marko Salmi, Ilmo Leivo, Juha Peltonen
Summary: This study investigated the immune microenvironment of cutaneous neurofibromas (cNFs) in patients with neurofibromatosis 1 (NF1). The results showed that cNFs have substantial populations of T cells and macrophages, which may be tumor-specific. T cell populations in cNFs were found to be different from those in the skin, and cNFs exhibited lower expression of proteins related to T cell-mediated immunity compared to the skin.
LABORATORY INVESTIGATION
(2024)