Review
Biochemistry & Molecular Biology
Saravanan Sekaran, Selvaraj Vimalraj, Lakshmi Thangavelu
Summary: TNAP is an essential enzyme involved in skeletal tissue mineralization and dephosphorylation of various physiological substrates. It plays vital roles in neuronal development, detoxification, anti-inflammatory processes, bile pH regulation, and maintaining the blood brain barrier. Understanding the mechanisms mediated by TNAP is crucial for minimizing off targeted effects and improving various pathological scenarios.
Article
Orthopedics
S. Jo, J. S. Lee, B. Nam, Y. L. Lee, H. Kim, E. Y. Lee, Y-S Park, T-H Kim
Summary: This study identified the presence of granular tissue and cartilage in the subchondral bone of patients with ankylosing spondylitis (AS), and found co-expression of the transcription factor SOX9 and matrix metalloproteinase 13 (MMP13) in these tissues. SOX9 plays an important role in inflammation and promotes mineralization features in entheseal tissue. These findings emphasize the importance of SOX9 in enthesis cells.
OSTEOARTHRITIS AND CARTILAGE
(2022)
Article
Immunology
Zena Chen, Xuqi Zheng, Xinyu Wu, Jialing Wu, Xiaomin Li, Qiujing Wei, Xi Zhang, Linkai Fang, Ou Jin, Jieruo Gu
Summary: The study found that the gut microbiome was significantly restored in AS patients after adalimumab therapy, with a higher abundance of Comamonas in non-responders compared to responders, although no statistical difference was observed. These results suggest the potential of gut microbiome as biomarkers for therapeutic evaluation.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Rheumatology
Michael Nissen, Benedicte Delcoigne, Daniela Di Giuseppe, Lennart Jacobsson, Merete Lund Hetland, Adrian Ciurea, Lucie Nekvindova, Florenzo Iannone, Nurullah Akkoc, Tuulikki Sokka-Isler, Karen Minde Fagerli, Maria Jose Santos, Catalin Codreanu, Manuel Pombo-Suarez, Ziga Rotar, Bjorn Gudbjornsson, Irene Van der Horst-Bruinsma, Anne Gitte Loft, Burkhard Moeller, Herman Mann, Fabrizio Conti, Gozde Yildirim Cetin, Heikki Relas, Brigitte Michelsen, Pedro Avila Ribeiro, Ruxandra Ionescu, Carlos Sanchez-Piedra, Matija Tomsic, Arni Jon Geirsson, Johan Askling, Bente Glintborg, Ulf Lindstroem
Summary: This multicenter study of axSpA patients found similar one-year treatment outcomes between TNFi monotherapy and csDMARD co-therapy. Heterogeneity across countries limited the identification of specific subgroups, such as peripheral arthritis, that may benefit from co-therapy.
Review
Clinical Neurology
Naiwen Hu, Xi Chen, Shanjuan Wang, Gangying Yuan, Qinqin Wang, Huae Shu, Hongsheng Sun
Summary: The polymorphisms of the TNF gene are associated with ankylosing spondylitis susceptibility, with certain gene variations showing significance in different ethnic populations. However, the results may vary between subgroups and the overall population.
EUROPEAN SPINE JOURNAL
(2021)
Review
Rheumatology
Francisco Airton Castro Rocha, Ana Carolina Matias Dinelly Pinto, Jailson Rodrigues Lopes, Atul Deodhar
Summary: TNFi is effective in preventing hip damage in AS patients, delaying the need for hip arthroplasty. Prompt initiation of TNFi is crucial for suspected hip involvement in AS patients.
CLINICAL RHEUMATOLOGY
(2021)
Article
Immunology
Jiayong Ou, Min Xiao, Yefei Huang, Liudan Tu, Zena Chen, Shuangyan Cao, Qiujing Wei, Jieruo Gu
Summary: This study investigated the systemic metabolic shifts associated with AS and TNF inhibitors treatment using LC-MS technology. A diagnostic panel comprising five metabolites was developed, capable of distinguishing HCs from AS with high accuracy. The study also revealed the metabolic pathways involved in AS and the effects of TNF inhibitors therapy on metabolism.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Rheumatology
Sizheng Steven Zhao, Gareth T. Jones, Gary J. Macfarlane, David M. Hughes, Robert J. Moots, Nicola J. Goodson
Summary: The study found that patients with multiple comorbidities in axSpA had poorer treatment outcomes when receiving TNFi therapy, with increased risk of treatment discontinuation, and poorer improvements in function and quality of life.
Article
Soil Science
Jiabao Li, Ting Xie, He Zhu, Jun Zhou, Chaonan Li, Wenjun Xiong, Lin Xu, Yanhong Wu, Zhili He, Xiangzhen Li
Summary: This study investigated the effects of alkaline and acid phosphatase activities on soil P availability in subalpine forests. The results showed that alkaline phosphatase activity played a key role in regulating soil P availability, primarily modulated by soil total N and N:P ratio.
Article
Medicine, General & Internal
Polina Kagan, Noy Horesh, Howard Amital, Avishai M. M. Tsur, Abdulla Watad, Arnon D. D. Cohen, Niv Ben-Shabat
Summary: AS patients in Israel have a higher overall risk of cancer compared to controls, especially for solid malignancies, CNS, kidney, and malignancy of unknown primary. Older age, comorbidity of diabetes and IBD, and treatment with DMARDs were associated with a higher risk of solid malignancies within AS patients, while NSAIDs treatment alone had a protective effect.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Endocrinology & Metabolism
Sungsin Jo, Seung Hoon Lee, Jinsung Park, Bora Nam, Hyunsung Kim, Jeehee Youn, Seunghun Lee, Tae-Jong Kim, Il-Hoon Sung, Sung Hoon Choi, Ye-Soo Park, Robert D. Inman, Tae-Hwan Kim
Summary: PDGFB plays a crucial role in the formation of enthesophytes in ankylosing spondylitis (AS) and is associated with spinal progression in AS. Increased levels of PDGFB were found in AS patients and correlated with radiographic progression. Inhibition of PDGFRB reduced PDGFB-induced bone mineralization and attenuated arthritis severity and enthesophyte formation in a mouse model. Regulating PDGFB/PDGFRB signaling could be a novel therapeutic strategy for AS.
JOURNAL OF BONE AND MINERAL RESEARCH
(2023)
Article
Endocrinology & Metabolism
Freja Pontan, Helena Hauta-alus, Saara Valkama, Jenni Rosendahl, Maria Enlund-Cerullo, Sture Andersson, Outi Makitie, Elisa Holmlund-Suila
Summary: This study investigated the concentrations of alkaline phosphatase (ALP) and the prevalence of hyperphosphatasemia in healthy children, and their association with vitamin D, growth, infections, and bone parameters. It was found that boys had higher ALP levels than girls, and hyperphosphatasemia was more common at 12 months. Hyperphosphatasemia was associated with body size, growth rate, and bone mineralization, but not with infections.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2023)
Review
Endocrinology & Metabolism
Konstantinos Makris, Chagigia Mousa, Etienne Cavalier
Summary: Alkaline phosphatases are isoenzymes that catalyze the hydrolysis of organic phosphate esters in the extracellular space. Zinc and magnesium are important co-factors for their biological activity. They are found in various tissues, but most are derived from the liver and bone in the bloodstream. However, their exact physiological function remains largely unknown.
CALCIFIED TISSUE INTERNATIONAL
(2023)
Article
Rheumatology
Kalliopi Klavdianou, Styliani Tsiami, Xenofon Baraliakos
Summary: Axial SpA is a rheumatic disease characterized by inflammation leading to bone formation and functional impairment. While TNF-alpha and IL-17 have shown efficacy as therapeutic targets in axSpA, clinical trials of anti-IL-23 monoclonal antibodies have yielded negative results, suggesting the existence of an IL-23 independent pathway. The Janus kinase (JAK) pathway is also a potential therapeutic target for further research in improving understanding and management of SpA pathogenesis.
Review
Clinical Neurology
Naiwen Hu, Dongxia Liu, Na Zhao, Xia Wang, Yanyan Bai, Hongsheng Sun
Summary: The study found significant associations between TNF-238, -308, -857, -1031, and -863 minor alleles and AS susceptibility in the HLA-B27 positive population. However, no association was found between TNF polymorphisms and AS in the HLA-B27-positive patients group and the HLA-B27-positive healthy controls group. Other gene SNPs may play a role in AS susceptibility in the HLA-B27-positive population.
EUROPEAN SPINE JOURNAL
(2021)
Review
Cardiac & Cardiovascular Systems
Goettsch Claudia, Strzelecka-Kiliszek Agnieszka, Bessueille Laurence, Quillard Thibaut, Mechtouff Laura, Pikula Slawomir, Canet-Soulas Emmanuelle, Jose Luis Millan, Fonta Caroline, Magne David
Summary: Cardiovascular calcification (CVC) is associated with increased morbidity and mortality, but there is currently no effective treatment available. Tissue-nonspecific alkaline phosphatase (TNAP) may be a promising target for limiting CVC, but its other physiological functions beyond mineralization also need further research.
CARDIOVASCULAR RESEARCH
(2022)
Article
Chemistry, Applied
Perrine Gelebart, Stephane Cuenot, Corinne Sinquin, Boris Halgand, Sophie Sourice, Catherine Le Visage, Jerome Guicheux, Sylvia Colliec-Jouault, Agata Zykwinska
Summary: This study investigated the ability of a bacterial exopolysaccharide called Infernan to bind and release BMP-2, and designed two delivery systems using a microfluidic approach. The results show that the release kinetics and bioactivity of BMP-2 are highly influenced by the ionic strength.
CARBOHYDRATE POLYMERS
(2022)
Article
Orthopedics
B. Bodic, M. A. Boutet, C. Boyer, B. Metayer, C. Vignes, J. Lesoeur, J. Veziers, V. Daguin, F. Haspot, Y. Maugars, F. Djouad, J. Guicheux, C. Vinatier
Summary: This study demonstrated the feasibility of inducing osteoarthritis (OA) in immunologically humanized mice by destabilization of the medial meniscus (DMM). The developed OA model could serve as a useful tool for bridging the gap between preclinical and clinical evaluation of immune interacting disease-modifying osteoarthritis drugs (DMOADs) and cell-based therapies.
OSTEOARTHRITIS AND CARTILAGE
(2022)
Article
Multidisciplinary Sciences
Nora Bouhsina, Cyrille Decante, Jean-Baptiste Hardel, Dominique Rouleau, Jerome Abadie, Antoine Hamel, Catherine Le Visage, Julie Lesoeur, Jerome Guicheux, Johann Clouet, Marion Fusellier
Summary: This study aims to determine the most suitable method for assessing lumbar intervertebral disc degeneration (IDD) in sheep by comparing three quantitative MRI sequences with Pfirrmann grading and histology. The results show that T1, T2, and T2* relaxation times measurements correlate well with the grading and histological scoring, indicating their accuracy and efficiency in evaluating IDD.
SCIENTIFIC REPORTS
(2022)
Review
Chemistry, Multidisciplinary
Constance Lesage, Marianne Lafont, Pierre Guihard, Pierre Weiss, Jerome Guicheux, Vianney Delplace
Summary: The osteochondral (OC) unit is essential for joint lubrication and bone constraint transmission. Currently, there is no curative treatment for OC defects, making OC regeneration a medical challenge. Over the past two decades, tissue engineering strategies have been proposed, but successful clinical translation has been limited. This systematic review provides insights into material-assisted strategies for OC regeneration, including therapeutic potential and evaluation methods.
Article
Biochemistry & Molecular Biology
Ekeveliny Amabile Veschi, Mayte Bolean, Luiz Henrique da Silva Andrilli, Heitor Gobbi Sebinelli, Agnieszka Strzelecka-Kiliszek, Joanna Bandorowicz-Pikula, Slawomir Pikula, Thierry Granjon, Saida Mebarek, David Magne, Jose Luis Millan, Ana Paula Ramos, Rene Buchet, Massimo Bottini, Pietro Ciancaglini
Summary: The study investigated the role of annexins in matrix vesicles-mediated bone mineralization, especially in the presence of type-I collagen. The results showed that AnxA6 may interact with phosphatidylserine to form a nucleational core, or with type-I collagen, though less efficiently, to induce the nucleation process.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medical Laboratory Technology
Laurence Bessueille, Lynn Kawtharany, Thibaut Quillard, Claudia Goettsch, Anne Briolay, Nirina Taraconat, Stephane Balayssac, Veronique Gilard, Saida Mebarek, Olivier Peyruchaud, Francois Duboeuf, Caroline Bouillot, Anthony Pinkerton, Laura Mechtouff, Rene Buchet, Eva Hamade, Kazem Zibara, Caroline Fonta, Emmanuelle Canet-soulas, Jose luis Millan, David Magne
Summary: This study found that the activation of tissue-nonspecific alkaline phosphatase (TNAP) precedes and predicts calcification in atherosclerotic plaques, and TNAP inhibition prevents plaque calcification and protects arteries from atherosclerosis. The study also discovered that TNAP inhibition reduces blood levels of cholesterol and triglycerides, and ameliorates plaque calcification, thereby protecting arteries.
TRANSLATIONAL RESEARCH
(2023)
Article
Cardiac & Cardiovascular Systems
Marina A. Heuschkel, Anne Babler, Jonas Heyn, Emiel P. C. van der Vorst, Marja Steenman, Maren Gesper, Ben A. Kappel, David Magne, Yann Goueffic, Rafael Kramann, Willi Jahnen-Dechent, Nikolaus Marx, Thibaut Quillard, Claudia Goettsch
Summary: This study compares two in vitro models of vascular smooth muscle cell (SMC) calcification, revealing distinct intracellular mechanisms triggered by OM and CaP. PKC plays a differential role in OM and CaP-induced SMC calcification, providing potential targets for pharmacological intervention in vascular calcification.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Engineering, Biomedical
Nathan Lagneau, Pierre Tournier, Fabien Nativel, Yves Maugars, Jerome Guicheux, Catherine Le Visage, Vianney Delplace
Summary: Currently, there is no curative treatment for osteoarthritis (OA), but using mesenchymal stromal cells (MSCs) to alleviate the inflammatory symptoms of OA is gaining attention. MSCs can respond to the pro-inflammatory microenvironment of an OA joint and secrete factors that have anti-inflammatory, anti-apoptotic, immunomodulatory, and pro-regenerative effects. However, the microenvironment of MSCs greatly influences their survival and bioactivity, and using tailored biomaterial scaffolds is crucial for the success of intra-articular MSC-based therapies.
Article
Cell Biology
Anais Defois, Nina Bon, Alexandre Charpentier, Melina Georget, Nicolas Gaigeard, Frederic Blanchard, Antoine Hamel, Denis Waast, Jean Armengaud, Ophelie Renoult, Claire Pecqueur, Yves Maugars, Marie-Astrid Boutet, Jerome Guicheux, Claire Vinatier
Summary: This study reveals a strong association between inflammation and metabolic dysregulation in osteoarthritic chondrocytes. Inflammation affects metabolic-related genes and leads to increased glycolysis and decreased mitochondrial respiration in osteoarthritic chondrocytes. These findings provide important insights for further understanding the mechanisms of osteoarthritis.
CELL COMMUNICATION AND SIGNALING
(2023)
Article
Engineering, Biomedical
Fabien Nativel, Audrey Smith, Jeremy Boulestreau, Charles Lepine, Julie Baron, Melanie Marquis, Caroline Vignes, Yoan Le Guennec, Joelle Veziers, Julie Lesoeur, Francois Loll, Boris Halgand, Denis Renard, Jerome Abadie, Benoit Legoff, Frederic Blanchard, Olivier Gauthier, Claire Vinatier, Anne des Rieux, Jerome Guicheux, Catherine Le Visage
Summary: Osteoarthritis is an inflammatory joint disease affecting cartilage, subchondral bone, and joint tissues. In this study, human adipose stromal cells were successfully encapsulated in alginate microgels and shown to retain their metabolic activity and ability to respond to inflammatory stimuli. When injected into a rabbit model of post-traumatic OA, the encapsulated cells exhibited properties matching those of non-encapsulated cells and showed a tendency toward decreased OA severity. These findings demonstrate the feasibility, safety, and efficacy of injecting cells encapsulated in microgels for long-term treatment of OA.
MATERIALS TODAY BIO
(2023)
Article
Chemistry, Multidisciplinary
Pierre Tournier, Garance Saint-Pe, Nathan Lagneau, Francois Loll, Boris Halgand, Arnaud Tessier, Jerome Guicheux, Catherine Le Visage, Vianney Delplace
Summary: This study introduces a new biomaterial concept, clickable dynamic bioinks. These bioinks use dynamic hydrogels that can be 3D-bioprinted and chemically modified via click and bioorthogonal reactions to allow a variety of post-printing modifications. These modifications include adjusting the bioink composition and stiffness, as well as promoting cell adhesion, and can be controlled over time and space for 4D bioprinting applications.
Review
Cell & Tissue Engineering
Emilie Hascoet, Frederic Blanchard, Claudine Blin-Wakkach, Jerome Guicheux, Philippe Lesclous, Alexandra Cloitre
Summary: Rheumatoid arthritis (RA) and periodontitis are chronic inflammatory diseases that lead to increased bone resorption. They have immunopathogenic similarities and a common inflammatory environment. Periodontal dysbiosis, triggered by periodontal pathogens, can promote or sustain bone resorption in inflamed joints in RA. Understanding the pathogenic mechanisms of osteoclasts and their precursors in these diseases could lead to the identification of new therapeutic targets for inflammatory bone resorption.
Meeting Abstract
Orthopedics
M. Georget, N. Bon, C. Vignes, J. Lesoeur, C. Boyer, A. Defois, A. Fouasson-Chailloux, G. Grimandi, J. Guicheux, C. Vinatier
OSTEOARTHRITIS AND CARTILAGE
(2022)
Article
Cardiac & Cardiovascular Systems
Laura Mechtouff, Monica Sigovan, Philippe Douek, Nicolas Costes, Didier Le Bars, Adeline Mansuy, Julie Haesebaert, Alexandre Bani-Sadr, Jeremie Tordo, Patrick Feugier, Antoine Millon, Stephane Luong, Salim Si-Mohamed, Diane Collet-Benzaquen, Emmanuelle Canet-Soulas, Thomas Bochaton, Claire Crola Da Silva, Alexandre Paccalet, David Magne, Yves Berthezene, Norbert Nighoghossian
Summary: This exploratory study assessed the potential of hybrid PET/MRI using F-18-NaF to simultaneously check microcalcification marker uptake and morphological criteria of vulnerability. The study found higher F-18-NaF uptake in culprit plaques compared to nonculprit plaques, but it was not associated with morphological criteria of vulnerability on MRI.
JOURNAL OF NUCLEAR CARDIOLOGY
(2022)
Article
Medicine, Research & Experimental
Marijn A. Scheijde-Vermeulen, Lennart A. Kester, Liset Westera, Bastiaan B. J. Tops, Friederike A. G. Meyer-Wentrup
Summary: This study aimed to evaluate the feasibility of integrating state-of-the-art sequencing techniques and flow cytometry into the diagnostic workup of pediatric lymphoma. The results showed that this integration is not only feasible but also provides additional diagnostic information.
LABORATORY INVESTIGATION
(2024)
Article
Medicine, Research & Experimental
Enrico Berrino, Sara Erika Bellomo, Anita Chesta, Paolo Detillo, Alberto Bragoni, Amedeo Gagliardi, Alessio Naccarati, Matteo Cereda, Gianluca Witel, Anna Sapino, Benedetta Bussolati, Gianni Bussolati, Caterina Marchi
Summary: Formalin-fixed paraffin-embedded (FFPE) samples are crucial for tissue-based analysis in precision medicine, but the quality of these samples can affect the reliability of sequencing data. The use of acid-deprived fixatives guarantees the highest DNA preservation and sequencing performance, enabling more complex molecular profiling of tissue samples.
LABORATORY INVESTIGATION
(2024)
Article
Medicine, Research & Experimental
Roope A. Kallionpaa, Sirkku Peltonen, Kim My Le, Eija Martikkala, Mira Jaaskelainen, Elnaz Fazeli, Pilvi Riihila, Pekka Haapaniemi, Anne Rokka, Marko Salmi, Ilmo Leivo, Juha Peltonen
Summary: This study investigated the immune microenvironment of cutaneous neurofibromas (cNFs) in patients with neurofibromatosis 1 (NF1). The results showed that cNFs have substantial populations of T cells and macrophages, which may be tumor-specific. T cell populations in cNFs were found to be different from those in the skin, and cNFs exhibited lower expression of proteins related to T cell-mediated immunity compared to the skin.
LABORATORY INVESTIGATION
(2024)
